scholarly journals Bloom Time Effect Depends on Muscle Type and May Determine the Results of pH and Color Instrumental Evaluation

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1282
Author(s):  
Damian Knecht ◽  
Kamil Duziński ◽  
Anna Jankowska-Mąkosa
Keyword(s):  
Side Surface ◽  
Regression Equations ◽  
Strong Correlations ◽  
Muscle Type ◽  
Instrumental Evaluation ◽  
Ph Values ◽  
Research Material ◽  
Longissimus Lumborum ◽  
Muscle Types ◽  
Growth Changes

The aim of this study was to determine the effect of 30 min bloom time and the type of muscle on pH and color parameters together with the possibility of estimating these measurements. The research material consisted of 270 samples from 6 muscle types: LD—Longissimus dorsi, LL—Longissimus lumborum, IL—Iliacus, SEM—Semimembranosus, CT—Cutaneous trunci, LTD—Latissimus dorsi. Measurements included pH and color of fresh pork at 0 min, and after 30 min bloom time. Bloom time influenced all analyzed parameters, although to a varying effect, depending on the muscle type. The lowest pH values were noted for dorsal-located muscles (LD, LL), then in the ham area (IL, SEM), and the highest values of the location on the side surface of the carcass (CT, LTD). The large increase in the proportion of L* and a* was observed for CT muscle (20–30%, the highest of all observed) and LTD (20–25%); for LD and LL the largest growth changes were observed for parameters b* (15–20%) and H* (20–30%). The lowest number of strong correlations was noted for LD and CT muscles, and the largest for SEM. A very good fit (R2 > 0.90) of regression equations was achieved in 7 cases. The presented results are an important contribution to the rapid and precise instrumental evaluation of pH and color.

scholarly journals The Drosophila indirect flight muscle myosin heavy chain isoform is insufficient to transform the jump muscle into a highly stretch-activated muscle type

2017 ◽  
Vol 312 (2) ◽  
pp. C111-C118 ◽  
Author(s):  
Cuiping Zhao ◽  
Douglas M. Swank
Keyword(s):  
Power Generation ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Flight Muscle ◽  
Fold Increase ◽  
Isometric Tension ◽  
Indirect Flight Muscle ◽  
Muscle Type ◽  
Myosin Heavy Chain Isoform ◽  
Muscle Types

Stretch activation (SA) is a delayed increase in force that enables high power and efficiency from a cyclically contracting muscle. SA exists in various degrees in almost all muscle types. In Drosophila, the indirect flight muscle (IFM) displays exceptionally high SA force production ( FSA), whereas the jump muscle produces only minimal FSA. We previously found that expressing an embryonic (EMB) myosin heavy chain (MHC) isoform in the jump muscle transforms it into a moderately SA muscle type and enables positive cyclical power generation. To investigate whether variation in MHC isoforms is sufficient to produce even higher FSA, we substituted the IFM MHC isoform (IFI) into the jump muscle. Surprisingly, we found that IFI only caused a 1.7-fold increase in FSA, less than half the increase previously observed with EMB, and only at a high Pi concentration, 16 mM. This IFI-induced FSA is much less than what occurs in IFM, relative to isometric tension, and did not enable positive cyclical power generation by the jump muscle. Both isometric tension and FSA of control fibers decreased with increasing Pi concentration. However, for IFI-expressing fibers, only isometric tension decreased. The rate of FSA generation was ~1.5-fold faster for IFI fibers than control fibers, and both rates were Pi dependent. We conclude that MHC isoforms can alter FSA and hence cyclical power generation but that isoforms can only endow a muscle type with moderate FSA. Highly SA muscle types, such as IFM, likely use a different or additional mechanism.

Mechanical properties of isolated human esophageal smooth muscle

1990 ◽  
Vol 258 (3) ◽  
pp. G338-G343 ◽  
Author(s):  
A. Tottrup ◽  
A. Forman ◽  
N. Uldbjerg ◽  
P. Funch-Jensen ◽  
K. E. Andersson
Keyword(s):  
Smooth Muscle ◽  
Esophagogastric Junction ◽  
Active Tension ◽  
Muscle Type ◽  
Optimum Length ◽  
Tension Development ◽  
Resting Tension ◽  
Muscle Types ◽  
Human Esophagus

Isolated smooth muscle strips from the human esophagus representing both the longitudinal and circular layers of the esophagogastric junction and the esophageal body were prepared. The strips were mounted in organ baths, and resting length was defined. By repeatedly increasing the length of the strips with 20% of resting length and recording values of resting and active tensions, length-tension relations for each muscle type were constructed. Only circular strips from the esophagogastric junction developed active, resting tension, disclosed by replacing the normal Ca2(+)-containing Krebs solution with Ca2(+)-free medium. Carbachol (10(-6) M) was used for submaximal activation of the contractile apparatus. At lengths between 180 and 260% of resting length, all strips reached optimum length (LO) where further elongation gave no further increase in active tension development. Repeated stimulations with carbachol was possible at a length of 200% of LO without affecting reproducibility. Determination of different collagen components revealed no differences between muscle types.

scholarly journals The Applicability of Total Color Difference ΔE for Determining the Blooming Time in Longissimus Lumborum and Semimembranosus Muscles from Holstein-Friesian Bulls at Different Ageing Times

2020 ◽  
Vol 10 (22) ◽  
pp. 8215
Author(s):  
Katarzyna Tkacz ◽  
Monika Modzelewska-Kapituła ◽  
Adam Więk ◽  
Zenon Nogalski
Keyword(s):  
Ageing Time ◽  
Color Difference ◽  
Muscle Type ◽  
Practical Applications ◽  
Holstein Friesian ◽  
Longissimus Lumborum ◽  
The Difference ◽  
Beef Color ◽  
Restaurant Owners ◽  
Beef Muscles

This study was conducted to determine the optimal blooming time in beef muscles based on ΔE, and to analyze the effects of muscle type and ageing time on beef color and blooming. Beef color was determined on freshly cut longissimus lumborum (LL, n = 8) and semimembranosus (SM, n = 8) muscles on days 1, 9, and 14 of ageing during 60 min blooming at 5 min intervals. It was found that ΔE0, representing the difference in color between freshly cut muscles and subsequently analyzed samples, supported the determination of the optimal blooming time, which varied across ageing times (15, 20, 25 min for the LL muscle, and 10, 15, 20 min for the SM muscle on days 1, 9, and 14 of ageing, respectively). Beef color was affected by both muscle type and ageing. The values of color parameters increased between days 1 and 9 of ageing. The results may have practical applications because beef should be presented to consumers and restaurant owners approximately 25 min after cutting, when its color has fully developed.

Quantitative Analysis of Iron and Silicon Concentrations in Iron Ore Concentrate Using Portable X-ray Fluorescence (XRF)

2019 ◽  
Vol 74 (1) ◽  
pp. 55-62 ◽  
Author(s):  
Shubin Zhou ◽  
Zhaoxian Yuan ◽  
Qiuming Cheng ◽  
David C. Weindorf ◽  
Zhenjie Zhang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Metal Concentration ◽  
Mineral Exploration ◽  
Ordinary Least Squares ◽  
Regression Equations ◽  
Strong Correlations ◽  
Environmental Evaluation ◽  
X Ray ◽  
Relative Errors ◽  
Archaeological Analysis

As a technique capable of rapid, nondestructive, and multi-elemental analysis, portable X-ray fluorescence (pXRF) has applications to mineral exploration, environmental evaluation, and archaeological analysis. However, few applications have been conducted in the smelting industry especially when analyzing the metal concentration in ore concentrate samples. This research analyzed the effectiveness of using pXRF in determining the metal concentration in Fe concentrate. For this proof of concept study, Fe ore samples dominated by Fe and Si were collected from the Northeastern University Mineral Processing Laboratory (Shenyang, China) and directly analyzed using pXRF, laboratory-based XRF, and titration methods. The compactness (density) of the ore concentrate was found to have very little effect on pXRF readings. The pXRF readings for Fe and Si were comparative to laboratory-based XRF results. Based on the strong correlations between the pXRF and XRF results (Fe: R2 > 0.99, Si: R2 > 0.96), linear calibrations were adopted to improve the accuracy of pXRF readings. Linear regression equations derived from the relations between XRF results and pXRF results of 21 Fe ore concentrate samples were used to calibrate the pXRF, and then validation was performed on five additional samples. Results from this preliminary study suggest that ordinary least squares (OLS) regression improves the accuracy dramatically, especially for Fe with relative errors (REs) decreasing to 0.03%–3.27% from 4.26%–8.32%. Consequently, pXRF shows strong promise for rapid, quantitative analysis of Fe concentration in Fe ore concentrate. Based on the results obtained in this study, a larger, more comprehensive study is warranted to confirm the results obtained.

scholarly journals Comparison of Nutritional and Meat Quality Characteristics between Two Primal Cuts from Aceh Cattle in Aceh Province, Indonesia

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Hamny Sofyan ◽  
Aryani Sismin Satyaningtijas ◽  
Cece Sumantri ◽  
Etih Sudarnika ◽  
Srihadi Agungpriyono
Keyword(s):  
Gene Expression ◽  
Muscle Fibers ◽  
Semitendinosus Muscle ◽  
Mstn Gene ◽  
Aceh Province ◽  
Longissimus Lumborum ◽  
Staining Methods ◽  
Muscle Types ◽  
Water Holding

The Aceh cattle are local Indonesian beef cattle that are farmed in Aceh Province. This type of cattle is one of the sources of meat for the Aceh people. This study aims to analyze the quality of two primal cuts (longissimus lumborum and semitendinosus muscle) from Aceh cattle based on the muscle microstructure characteristics and MSTN gene expression. This study used a sample of longissimus lumborum and semitendinosus muscles from 18 adult male Aceh cattle with the age of 2–2.5 years and a BCS of 3.24. Muscle samples were obtained shortly after the cattle were slaughtered in slaughterhouses in Banda Aceh and Aceh Besar districts. Muscle microstructure analysis was performed using the HE, Masson’s trichrome, and immunohistochemistry staining methods, while the MSTN gene expression analysis was performed using the qPCR method. The analysis of the physical quality of meat includes pH, meat color, fat color, cooking loss, water holding capacity, and WBSF value. The results showed that the area of LL muscle fibers was smaller than that of ST with relatively the same diameter. Both muscles were dominated by fast fibers with a percentage of 82.37% (LL muscle) and 91.80% (ST muscle). The area and composition of the type of muscle fibers are the main factors that influence the tenderness of Aceh beef. A higher distribution of collagen was found in ST muscles than in LL muscles. MSTN gene expression in both muscle types was relatively the same. Aceh cattle have large muscle fibers and are dominated by fast fibers with a high percentage, resulting in a low level of the tenderness of Aceh beef. However, the level of tenderness of Aceh beef is still in accordance with the cooking preparation of original and favorite cuisine of Aceh people.

CONVERSION OF ORGANIC SOIL pH VALUES MEASURED IN WATER, 0.01M CaCl2 or 1N KCl

1981 ◽  
Vol 61 (4) ◽  
pp. 577-579 ◽  
Author(s):  
W. VAN LIEROP
Keyword(s):  
Soil Ph ◽  
Dilution Effect ◽  
Organic Soil ◽  
Organic Soils ◽  
Regression Equations ◽  
Average Difference ◽  
Ph Values ◽  
Water Ph

Regression equations were derived for converting pH values of organic soils determined by five procedures. Data were obtained by measuring the pH of 30 soils using the following volumetric ratios and solutions: 1:1, soil to water; 1:2 and 1:4, soil to 0.01M CaCl2; and 1:2 and 1:4 soil to 1N KCl. Average pH values measured in 0.01M CaCl2 and 1N KCl were 0.44 and 0.70 pH units lower than those measured in water (pH 5.21). Converting data by merely adding or subtracting the average difference between methods was not as accurate as using appropriate regression equations. These equations are provided in the text and indicated that differences between soil pH values measured by different procedures increased as soil pH increased. Similar pH values were found with the 1:2 and 1:4 soil to 0.1M CaCl2 solution ratios, though a small dilution effect was observed when 1N KCl was used at these ratios.

Effect of cold acclimation on troponin I isoform expression in striated muscle of rainbow trout

2012 ◽  
Vol 303 (2) ◽  
pp. R168-R176 ◽  
Author(s):  
Sarah L. Alderman ◽  
Jordan M. Klaiman ◽  
Courtney A. Deck ◽  
Todd E. Gillis
Keyword(s):  
Rainbow Trout ◽  
Cold Acclimation ◽  
Troponin I ◽  
Striated Muscle ◽  
Protein Isoforms ◽  
Specific Gene ◽  
Muscle Type ◽  
Gene Transcripts ◽  
Muscle Types ◽  
Isoform Expression

In vertebrates each of the three striated muscle types (fast skeletal, slow skeletal, and cardiac) contain distinct isoforms of a number of different contractile proteins including troponin I (TnI). The functional characteristics of these proteins have a significant influence on muscle function and contractility. The purpose of this study was to characterize which TnI gene and protein isoforms are expressed in the different muscle types of rainbow trout ( Oncorhynchus mykiss) and to determine whether isoform expression changes in response to cold acclimation (4°C). Semiquantitative real-time PCR was used to characterize the expression of seven different TnI genes. The sequence of these genes, cloned from Atlantic salmon ( Salmo salar) and rainbow trout, were obtained from the National Center for Biotechnology Information databases. One-dimensional gel electrophoresis and tandem mass spectrometry were used to identify the TnI protein isoforms expressed in each muscle type. Interestingly, the results indicate that each muscle type expresses the gene transcripts of up to seven TnI isoforms. There are significant differences, however, in the expression pattern of these genes between muscle types. In addition, cold acclimation was found to increase the expression of specific gene transcripts in each muscle type. The proteomics analysis demonstrates that fast skeletal and cardiac muscle contain three TnI isoforms, whereas slow skeletal muscle contains four. No other vertebrate muscle to date has been found to express as many TnI protein isoforms. Overall this study underscores the complex molecular composition of teleost striated muscle and suggests there is an adaptive value to the unique TnI profiles of each muscle type.

scholarly journals Relationship between Air Temperature Change and Southern Baltic Coastal Lagoons Ice Conditions

Atmosphere ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 931
Author(s):  
Józef Piotr Girjatowicz ◽  
Małgorzata Świątek
Keyword(s):  
Air Temperature ◽  
Coastal Lagoons ◽  
Statistical Significance ◽  
Prognostic Significance ◽  
Correlation Coefficients ◽  
Regression Equations ◽  
Strong Correlations ◽  
Baltic Coast ◽  
Ice Conditions ◽  
Southern Baltic

The relationship between air temperature (mainly winter, December-March) in Świnoujście, Gdynia, and Elbląg and ice parameters (dates of the first ice and disappearance of the last ice, the length of the ice season, number of days with ice, maximum ice height) of southern Baltic coastal lagoons (Szczecin, Puck, and Vistula) was investigated. Trends in these parameters were determined, too. The observation material comes from the archives of the Institute of Meteorology and Water Management and spanned the winters from 1950/51 through to 2019/20. Relationships between the selected ice parameters for the study basins and the values of air temperature were examined using correlation and regression methods. The regression equations and trends, as well as their correlation and determination coefficients, were determined. The statistical significance of these relationships was examined using the Fisher-Snedecor test. Strong correlations between ice parameters values and air temperature were obtained, characterized by high values of both correlation coefficients and statistical significance. All trends of ice parameters indicate mitigation of ice conditions. An acceleration in both temperature and ice condition mildening occurred in the late 1980s, and especially in the last years of the study period. These trends, except the first ice date, are statistically significant, some even at α < 0.001. The length of the ice season becomes significantly shorter, the number of days with ice and the maximum thickness is smaller, and the last ice is disappearing early. An increase in the correlation and determination coefficients and a characterized trend of ice parameters values towards the East was found. It shows the increased impact of a warming climate in this direction on the southern Baltic coast. Strong correlations and trends may be of prognostic significance.

scholarly journals The effect of genotype and muscle type on the physico-chemical characteristics and taurine, carnosine and L-carnitine concentration in lamb meat

2020 ◽  
Vol 63 (2) ◽  
pp. 423-430
Author(s):  
Aurelia Radzik-Rant ◽  
Witold Rant ◽  
Gabriela Sosnowiec ◽  
Marcin Świątek ◽  
Roman Niżnikowski ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Gluteus Medius ◽  
Chemical Characteristics ◽  
Bioactive Components ◽  
Muscle Type ◽  
Lamb Meat ◽  
Physico Chemical ◽  
Longissimus Lumborum ◽  
Chemical Traits

Abstract. An experiment was conducted to determine the chosen bioactive components and physico-chemical characteristics of lamb meat of different animal genotypes and the muscle types. The 22 ram lambs of Polish Merino (PM) and 22 crossbreeds of Polish Merino × Berrichone du Cher (PMB) were fattened to achieve their slaughter weight of 40 kg. After slaughter, the carcasses were kept at 4 ∘C for 24 h. Then, the samples of longissimus lumborum (LL) and gluteus medius (GM) muscle were collected to analyse the physico-chemical traits; fatty acid profile; and concentrations of taurine, carnosine, L-carnitine. The GM muscle compared to LL had the higher value (P < 0.05) of L* and a lower value (P < 0.05) of b* and H* both in PM and PMB lambs. The value of expressed juice was lower (P < 0.05) in both LL and GM muscles of PM lambs. A higher amount (P < 0.05) of collagen was found in LL muscle compared to GM both in PM and PMB lambs. The GM muscle of PM lambs showed higher (P < 0.05) conjugated linoleic acid (CLA) content, as well as higher total polyunsaturated acids (PUFAs), PUFA n-6, and PUFA n-3 (P < 0.05). The GM muscle was characterized by a higher (P < 0.05) content of taurine, while in the LL muscle there was a higher amount (P < 0.05) of carnosine. A larger amount (P < 0.05) of L-carnitine was found in GM muscle but only within PMB lambs. The obtained results showed a greater impact of the lamb's genotype on the physical characteristics of meat than on its chemical composition and the content of bioactive components. The muscle type had an effect on meat colour; collagen content; fatty acid profile; and amount of taurine, carnosine, and L-carnitine present.

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