scholarly journals Molecular Characteristics of Enterococcus faecalis and Enterococcus faecium from Bulk Tank Milk in Korea

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 661
Author(s):  
Sunghyun Yoon ◽  
Young Ju Lee
Keyword(s):  
Antimicrobial Resistance ◽  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Virulence Genes ◽  
Dairy Herd ◽  
Molecular Characteristics ◽  
Cross Contamination ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
Bulk Tank

Enterococci are considered to be environmental mastitis-causing pathogens that can easily spread antimicrobial resistance or virulence genes via horizontal transfer. In this study, the molecular characteristics of enterococci from bulk tank milk were investigated to assess the importance of dairy herd management. A total of 338 enterococci (305 Enterococcus faecalis and 33 Enterococcus faecium) were isolated from 1584 batches of bulk tank milk samples from 396 farms affiliated with four dairy companies in Korea, and significant differences (40.6–79.7%) (p < 0.05) in the prevalence of enterococci were observed in the samples from different companies. Enterococci showed the highest resistance to tetracycline (TET) (73.4%), followed by doxycycline (DOX) (49.7%) and erythromycin (ERY) (46.2%), while two enterococci isolates showed resistance to vancomycin (VAN). Among 146 tetracycline (TET) and ERY-resistant enterococci, each 50 (19.4%) enterococci carried combination-resistance and transposon gene types erm(B) + tet(M) + IntTn and erm(B) + tet(L) + tet(M) + IntTn, respectively. The virulence genes such as ace (99.0%), efaA (97.7%), cad1 (95.7%), and gelE (85.9%) were highly conserved in E. faecalis and significantly predominated over E. faecium (p < 0.001). Our results indicate that pathogens from bulk tank milk can also become a reservoir for the dissemination of antimicrobial resistance and virulence factors through cross-contamination processes.

scholarly journals Characteristics of High-Level Aminoglycoside-Resistant Enterococcus faecalis Isolated from Bulk Tank Milk in Korea

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1724
Author(s):  
Hyo Jung Kang ◽  
Sunghyun Yoon ◽  
Koeun Kim ◽  
Young Ju Lee
Keyword(s):  
Antimicrobial Resistance ◽  
Enterococcus Faecalis ◽  
Virulence Factors ◽  
Virulence Genes ◽  
Multidrug Resistant ◽  
Management Program ◽  
Bulk Tank Milk ◽  
Antimicrobial Resistance Genes ◽  
Bulk Tank ◽  
High Level

Enterococci, which are considered environmental mastitis-causing pathogens, have easily acquired aminoglycoside-resistant genes that encode various aminoglycoside-modifying enzymes (AME). Therefore, this study was conducted to compare the distribution of high-level aminoglycoside-resistant (HLAR) and multidrug-resistant (MDR) Enterococcus faecalis (E. faecalis) bacteria isolated from bulk tank milk in four dairy companies in Korea. Moreover, it analyzed the characteristics of their antimicrobial resistance genes and virulence factors. Among the 301 E. faecalis bacteria studied, 185 (61.5%) showed HLAR with no significant differences among the dairy companies. Furthermore, 129 (69.7%) of the 185 HLAR E. faecalis showed MDR without significant differences among companies. In contrast, HLAR E. faecalis from companies A, B, and C were significantly higher in resistance to the four classes than those in company D, which had the highest MDR ability against the three antimicrobial classes (p < 0.05). In addition, in the distribution of AME genes, 72 (38.9%) and 36 (19.5%) of the isolates carried both aac(6′)Ie-aph(2″)-la and ant(6)-Ia genes, and the ant (6)-Ia gene alone, respectively, with significant differences among the companies (p < 0.05). In the distribution of virulence genes, the ace (99.5%), efa A (98.9%), and cad 1 (98.4%) genes were significantly prevalent (p < 0.05). Thus, our results support that an advanced management program by companies is required to minimize the dissemination of antimicrobial resistance and virulence factors.

scholarly journals Detection of the Phenicol–Oxazolidinone Resistance Gene poxtA in Enterococcus faecium and Enterococcus faecalis from Food-Producing Animals during 2008–2018 in Korea

2020 ◽  
Vol 8 (11) ◽  
pp. 1839
Author(s):  
Seok-Hyeon Na ◽  
Dong-Chan Moon ◽  
Mi-Hyun Kim ◽  
Hee-Young Kang ◽  
Su-Jeong Kim ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Ribosomal Protein ◽  
Resistance Gene ◽  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Molecular Characteristics ◽  
Pfge Analysis ◽  
Linezolid Resistance

We aimed to investigate the presence of the phenicol–oxazolidinone resistance gene poxtA in linezolid-resistant enterococci from food-producing animals and analyze its molecular characteristics. We collected 3941 Enterococcus faecium and 5088 E. faecalis isolates from all provinces of South Korea from 2008 to 2018. We found linezolid resistance in 0.79% (94/3941) of E. faecium and 1.22% (62/5088) of E. faecalis isolates. Overall, 23.1% (36/156) of the linezolid-resistant isolates had the poxtA gene, including 31 E. faecium and five E. faecalis isolates. The poxtA-positive enterococci were mainly isolated from chicken (86.1%; 26/36). Fifteen poxtA-harboring isolates co-carried another linezolid-resistance gene, optrA. Eight E. faecium isolates had an N130K mutation in the ribosomal protein L4, while no mutations were observed in E. faecalis isolates. The poxtA gene was transferred into 10 enterococci by conjugation. Multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) analysis indicated that poxtA-carrying isolates were heterogeneous. Three E. faecium isolates belonged to CC17 (ST32, ST121, and ST491). To our knowledge, this is the first report on the poxtA gene in Korea. Prudent use of antimicrobials and active surveillance on antimicrobial resistance are urgently needed to reduce the risk of dissemination of the linezolid-resistant isolates in humans and animals.

scholarly journals BULK TANK SOMATIC CELL COUNT IN MILK SAMPLES FROM STATE OF PARANÁ

2014 ◽  
Vol 19 (1) ◽  
Author(s):  
Newton Pohl Ribas ◽  
Paulo Rossi Junior ◽  
Humberto Gonzalo Monardes ◽  
Uriel Vinicius Cotarelli Andrade ◽  
Altair Antonio Valotto ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Fixed Effects ◽  
Somatic Cell Count ◽  
Dairy Herd ◽  
The State ◽  
Scientific Cooperation ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
Bulk Tank

This research studied somatic cell count in bulk tank milk samples (BTSCC) from the state of Paraná, Brazil, at the Milk Quality Laboratory of the Dairy Herd Analysis Service of the Holstein Association of Paraná, the result of technical and scientific cooperation between UFPR and McGill University of Canada. A total of 1,950,034 bulk tank milk samples from ten regions of the state of Paraná were analyzed between January 2005 and April 2012 and were studied using PROC GLM (SAS, version 9.3). Fixed effects were the month and year of analysis, region and age of the sample. Means and standard deviations of BTSCC were 553,519 ± 545,532 cells/ml, respectively. All fixed effects were statistically significant (P<0.01). Highest values for BTSCC are observed in the month of February (554,000 cells/ml ± 1.45) and lowest values in September (450,000 cells/ml ± 1.47). Similarly, the highest values were found in the year 2010 (567,000 cells/ml ± 1.16), the lowest BTSCC was found in 2012 (444,000 cells/ml ± 1.57). The region effect was also significant with the highest values found in the South Center/ Guarapuava (668,000 cells/ml ± 0.87) and the lowest in the Southwest/ Francisco Beltrão (359,000 cells/ml ± 2.00). Both variables showed a reduction of their values with increasing age of the sample, from 518,000 ± 1.08 to 472,000 cells/ml ± 2.14 between the first and the seventh day, for the BTSCC. Coefficient of variation for BTSCC was 96.10%. The R² was 0.39 for BTSCC.

Dissemination and Characteristics of High-Level Erythromycin-Resistant Enterococcus Faecalis From Bulk Tank Milk of Dairy Companies in Korea

2021 ◽  
Author(s):  
Yu Jin Lee ◽  
Koeun Kim ◽  
Young Ju Lee
Keyword(s):  
Enterococcus Faecalis ◽  
Resistance Genes ◽  
Virulence Genes ◽  
Bovine Mastitis ◽  
Bulk Tank Milk ◽  
Significant Difference ◽  
Bulk Tank ◽  
Environmental Pathogens ◽  
High Level ◽  
Genotypic Characteristics

Abstract Background: Enterococci are environmental pathogens that can cause bovine mastitis and macrolides are widely used for the treatment of bovine mastitis caused by staphylococci and streptococci/enterococci. The aim of this study was performed to compare the phenotypic and genotypic characteristics of high-level erythromycin-resistant (HLER) Enterococcus faecalis (E. faecalis) collected from bulk tank milk of four dairy companies (A, B, C, and D) in Korea. Results: Although isolates from company D showed the highest prevalence of E. faecalis, the prevalence of HLER E. faecalis in company A (73.1%) and C (57.0%) was significantly higher than company D (33.9%) (P < 0.05). A total of 149 HLER E. faecalis isolates showed high rates of resistance to tetracycline (93.3%), followed by doxycycline (70.0%), and chloramphenicol (48.3%). In the distribution of macrolides resistance genes, 147 (98.7%) isolates carried ermB gene alone, and two isolates carried both ermA and ermB genes. No isolates carried ermC, msrA, msrC, or mef genes. In the distribution of other resistance genes, 72 (48.3%) and 60 (40.3%) isolates carried both tetM and tetL genes, and tetM gene alone, respectively, and 38 (25.5%) isolates carried optrA gene. For aminoglycosides resistance genes, the prevalence of both aac(6′)Ie-aph(2″)-la and ant(6′)-Ia genes (43.0%) was the highest. Moreover, 104 (70.0%) isolates harbored Int-Tn gene carrying the Tn916/1545-like transposon. Although the distribution of ermB gene showed no significant difference between the dairy companies, the prevalence of other resistance genes and transposons showed a significant difference between the dairy companies (P < 0.05). Virulence genes, such as ace (99.3%), cad1 and efaA (each 98.7%), and gelE (83.9%), were also highly conserved in the 149 HLER E. faecalis isolates. Conclusions: Our results indicated that HLER E. faecalis isolates from bulk tank milk showed significant differences in phenotypic and genotypic characteristics between the dairy companies. In addition, the prevalence of resistance genes and virulence factors was also high in HLER E. faecalis isolates.

scholarly journals Prevalence, antibiotic resistance, virulence and genetic diversity of Staphylococcus aureus isolated from bulk tank milk samples of U.S. dairy herds

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Kruthika Patel ◽  
Sandra M. Godden ◽  
Erin E. Royster ◽  
Brian A. Crooker ◽  
Timothy J. Johnson ◽  
...  
Keyword(s):  
Public Health ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Dairy Cows ◽  
Virulence Genes ◽  
Dairy Herds ◽  
Drug Resistant ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
Bulk Tank

Abstract Background Colonization of dairy cows by Staphylococcus aureus (S. aureus), especially those which are multi-drug resistant and toxin producing, is a concern for animal health and well-being as well as public health. The objective of this study was to investigate the prevalence, antibiotic resistance, gene content and virulence determinants of S. aureus in bulk tank milk samples (BTM) from U.S. dairy herds. Results BTM samples were collected, once in winter and once in summer, from 189 U.S. dairy herds. Of 365 BTM samples cultured, the sample and herd prevalence of S. aureus in BTM was 46.6% (170 of 365 samples) and 62.4% (118 of 189 herds), respectively. Among a subset of 138 S. aureus isolates that were stored for further analysis, 124 were genome sequenced after being confirmed as S. aureus using phenotypic tests. The most commonly identified antimicrobial resistance-associated gene was norA (99.2%) and mecA gene responsible for methicillin resistance (MRSA) was identified in one isolate (0.8%). The most frequently detected putative virulence genes were aur (100%), hlgB (100%), hlgA, hlgC, hlb (99.2%), lukE (95.9%) and lukD (94.3%). In the 53 staphylococcal enterotoxin positive isolates, sen (37.9%), sem (35.5%), sei (35.5%) and seg (33.1%) were the most frequently detected enterotoxin genes. Among the 14 sequence types (ST) and 18 spa types identified, the most common was ST2187 (20.9%) and t529 (28.2%), respectively. The most predominant clone was CC97 (47.6%) followed by CC unknown (36.3%). The single MRSA isolate belonged to ST72-CC8, spa type t126 and was negative for the tst gene but harbored all the other virulence genes investigated. Conclusion Our findings indicated a high prevalence of S. aureus in BTM of U.S. dairy herds, with isolates showing little evidence of resistance to antibiotics commonly used to treat mastitis. However, isolates often carried genes for the various enterotoxins. This study identified predominant genetic clones. Despite lower prevalence, the presence of MRSA and multi-drug resistant strains in BTM poses a significant risk to animal and public health if their number were to increase in dairy environment. Therefore, it is necessary to continuously monitor the use of antibiotics in dairy cows.

scholarly journals Arcobacter spp. in raw milk from vending machines in Piedmont and occurrence of virulence genes in isolates

2019 ◽  
Vol 8 (4) ◽  
Author(s):  
Amaranta Traversa ◽  
Silvia Gallina ◽  
Francesca Martucci ◽  
Cvetelina Boteva ◽  
Elisa Baioni ◽  
...  
Keyword(s):  
Virulence Genes ◽  
Foodborne Pathogen ◽  
Dairy Farms ◽  
Raw Milk ◽  
Human Consumption ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
Vending Machines ◽  
Bulk Tank ◽  
P Type

Arcobacter spp. has been recognized as an emerging foodborne pathogen and a hazard to human health. In the dairy chain, it has been isolated from different sources, nevertheless data on Arcobacter occurrence in raw milk provided by vending machines are few. This study aimed to identify potentially pathogenic Arcobacter spp. in raw milk intended for human consumption sold through vending machines located in Piedmont. In an 8-month period, 37 raw milk samples were collected from 24 dairy farms: 12 (32,4%) were collected directly in farm from bulk tank milk and 25 (67,6%) from vending machines. Eight (21,6%) out of the 37 milk samples and 7 (29,2%) out of the 24 dairy farms were positive for Arcobacter spp. by culture examination. Four (16%) out of the 25 samples from vending machines and 4 (33,3%) out of the 12 samples from bulk tank milk were positive. All 8 isolates were identified as A. butzleri both by MALDI-TOF MS and multiplex end-point PCR. According to the detection of virulence genes, a total of four Patho-types were highlighted: 5 isolates in P-type 1 and only one isolate for each of the P-types 2-3-4. A. butzleri isolates carrying encoding virulence factors genes were isolated from raw milk intended for human consumption: these findings strengthen the compulsory consumption after boiling as required by current legislation and suggest the need of enlarging the analytical investigations to other microorganisms not yet included in the food safety criteria.

scholarly journals Determination of the occurrence of plasmid virulence genes (spv) and antibiotic resistance of Salmonella strains isolated from raw milk

2018 ◽  
Vol 74 (1) ◽  
pp. 59-64 ◽  
Author(s):  
AGNIESZKA WISZNIEWSKA-ŁASZCZYCH ◽  
BARBARA ZDRODOWSKA ◽  
BEATA WYSOK ◽  
JOANNA SZTEYN ◽  
KATARZYNA LIEDTKE ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Virulence Genes ◽  
Raw Milk ◽  
Molecular Methods ◽  
Frequency Of Occurrence ◽  
Salmonella Spp ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
Bulk Tank

The aim of the study was to measure the frequency of occurrence of Salmonella spp. in raw milk, to identify their serotype, as well as to determine their antibiotic resistance and the presence of Salmonella plasmid virulence (spv) genes. Out of 300 bulk tank milk samples, 5.3% were contaminated with Salmonella spp. All strains isolated belonged to the serovar S enteritidis, as confirmed by serotyping and molecular methods. The presence of spv genes was determined by PCR. Spv genes were present, in different patterns, in all strains tested. SpvA gene was present in all isolates (100%), spvB in 56.25%, spvC in 62.5%, spvD in 75%, and spvR in 56.25%. Antibiotic resistance was evaluated according to the NCCLS recommendations. All isolates were sensitive to ciprofloxacin (CIP), gentamicin (GE), and chloramphenicol (CH). Thirteen strains were resistant to ampicillin (AMP), 8 to erythromycin (E), 1 to doxycycline (DO), and 1 to tetracycline (TE). Different frequency of occurrence of the spv genes in Salmonella strains isolated from raw milk demonstrates their high adaptability. As many as 87.5% of isolates showed resistance to at least one of the antibiotics tested....

The Use of Bulk Tank Milk Samples to Monitor Trends in Antimicrobial Resistance on Dairy Farms

2007 ◽  
Vol 4 (4) ◽  
pp. 397-407 ◽  
Author(s):  
Anna Catharina B. Berge ◽  
Staci C. Champagne ◽  
Regina M. Finger ◽  
William M. Sischo
Keyword(s):  
Antimicrobial Resistance ◽  
Dairy Farms ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
Bulk Tank

Use of Bulk Tank and Milk Filter Cultures in Screening for Streptococcus agalactiae and Coagulase-positive Staphylococci

1991 ◽  
Vol 54 (11) ◽  
pp. 848-851 ◽  
Author(s):  
PAUL C. BARTLETT ◽  
GAY Y. MILLER ◽  
SUE E. LANCE ◽  
LAWRENCE E. HEIDER
Keyword(s):  
Streptococcus Agalactiae ◽  
Dairy Herd ◽  
Sampling Scheme ◽  
Screening Tests ◽  
Dairy Herds ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
Bulk Tank ◽  
Low Sensitivity ◽  
Coagulase Positive Staphylococci

The use of a single bulk tank milk culture and a single milk filter culture was studied for their suitability as screening tests for coagulase-positive staphylococci and Streptococcus agalactiae. Bulk tank and bulk tank milk filter cultures were compared to quarter milk cultures taken from individual cows at 49 Ohio dairy herds selected from all Ohio dairy herds by a stratified random sampling scheme. Individual cow quarter milk samples were collected from a sample of all milking cows using a sampling scheme designed to detect an organism present in 2% of quarters, with 95% confidence intervals between 1 and 3%. Seventeen (35%) herds had one or more cows positive for S. agalactiae and 34 (69%) had one or more cows positive for coagulase-positive staphylococci. Using the results of individual cow sampling as the standard, the sensitivity for S. agalactiae was estimated as 23.5% for a single milk filter sample and 35.3% for a single bulk tank milk sample. The sensitivity for coagulase-positive staphylococci was estimated as 52.9% for a single milk filter culture and 41.2% for a single bulk tank milk culture. Based on these results and those of others, it appears that a single bulk tank or milk filter sample has a relatively low sensitivity for both coagulase-positive staphylococci and S. agalactiae, making these poor screening tests for the presence of these pathogens within a dairy herd.

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