scholarly journals Processing Matters in Nutrient-Matched Laboratory Diets for Mice—Energy and Nutrient Digestibility

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 523
Author(s):  
Linda F. Böswald ◽  
Jasmin Wenderlein ◽  
Reinhard K. Straubinger ◽  
Sebastian Ulrich ◽  
Ellen Kienzle
Keyword(s):  
Laboratory Animal ◽  
Nutrient Utilization ◽  
Nutrient Digestibility ◽  
Intestinal Microbiome ◽  
Total Dietary Fiber ◽  
Starch Gelatinization ◽  
Microbiome Research ◽  
Carbohydrate Digestibility ◽  
Pelleted Diet ◽  
Matter Energy

Starch gelatinization is a major determinant of carbohydrate digestibility and varies with diet processing. Laboratory rodent diets are often marketed as identical, but are sold in different forms, regardless of the markedly higher starch gelatinization in extruded than in pelleted diets. Our hypothesis was that this would impact energy and nutrient digestibility in mice fed pellets or extrudate, respectively. Trial 1 showed that feeding C57BL/6 mice a standard maintenance diet in extruded form results in a significantly higher digestibility of organic matter, energy, and carbohydrates than the identical diet in pelleted form. The replication of the experiment, however, revealed a variation between batches of the same pelleted diet regarding starch and total dietary fiber contents. Given the significant differences in diet digestibility and the potential impacts of digestibility on nutrient utilization, the intestinal microbiome, and intermediary metabolism, trials performed with differently processed diets are not comparable. This might partly explain failures to reproduce results, especially in gastrointestinal or microbiome research. Considering this impact on experimental animals, the degree of starch gelatinization should be declared in the diet information for laboratory animal diets. The differences between batches of laboratory animal diets as observed in the pellets are not acceptable.

scholarly journals Effect of ancient grains and grain-free carbohydrate sources on extrusion parameters and nutrient utilization by dogs

2019 ◽  
Vol 97 (9) ◽  
pp. 3758-3767 ◽  
Author(s):  
Julia Guazzelli Pezzali ◽  
Charles Gregory Aldrich
Keyword(s):  
Experimental Design ◽  
Dry Matter ◽  
Mechanical Energy ◽  
Nutrient Utilization ◽  
Extrusion Process ◽  
Nutrient Digestibility ◽  
Total Dietary Fiber ◽  
Randomized Experimental Design ◽  
Gross Energy ◽  
The Impact

Abstract The aim of this study was to evaluate the impact of ancient grain and grain-free carbohydrate sources on extrusion process, nutrient utilization, and palatability by dogs. Two maintenance dog diets were formulated with same proportions of carbohydrates: 1) ancient grain diet (AG) with spelt, millet, and sorghum; and 2) grain-free diet (GF) which had potato, peas, and tapioca starch. Experimental diets were extruded over 5 replicates in a completely randomized experimental design. Digestibility was carried out with 12 dogs in a switch-back experimental design. The GF diet required 22.6 and 25.9% more (P < 0.05) specific mechanical energy and in-barrel moisture input, respectively, than AG to produce kibbles out of the extruder with similar bulk density (P > 0.05). After drying, GF kibbles were less dense and more expanded, but harder than AG kibbles (P < 0.05). Dogs preferred GF over AG in the palatability assessment of uncoated kibbles. Apparent nutrient digestibility of dry matter, organic matter, gross energy, crude protein, and crude fat were not affected by treatment (P > 0.05). However, total dietary fiber (TDF) digestibility was 31.9% greater for dogs fed GF (P < 0.05). Moreover, wet fecal output was higher, and fecal dry matter was lower for dogs under GF (P < 0.05). The results demonstrated that GF and AG diets behaved differently during extrusion, but were similarly utilized by dogs, with exception of TDF. Thus, fiber content of grain-free diets should be monitored to maximize fecal quality.

scholarly journals Processed By-Products from Soy Beverage (Okara) as Sustainable Ingredients for Nile Tilapia (O. niloticus) Juveniles: Effects on Nutrient Utilization and Muscle Quality

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 590
Author(s):  
Glenise B. Voss ◽  
Vera Sousa ◽  
Paulo Rema ◽  
Manuela. E. Pintado ◽  
Luísa M. P. Valente
Keyword(s):  
Nile Tilapia ◽  
Lactobacillus Rhamnosus ◽  
Protein Digestibility ◽  
Nutrient Utilization ◽  
Nutrient Digestibility ◽  
Fish Growth ◽  
Muscle Quality ◽  
Whole Body ◽  
Hypertrophic Growth ◽  
Bifidobacterium Animalis

The apparent digestibility coefficients (ADCs) of differently processed okara meals were assessed in Nile tilapia diets: dried okara not autoclaved (FOK), dried okara autoclaved (AOK), okara hydrolyzed with Alcalase (ALOK) or Cynara cardunculus proteases (CYOK), and hydrolyzed okara fermented with lactic bacteria: Lactobacillus rhamnosus R11 (CYR11OK) or Bifidobacterium animalis ssp. lactis Bb12 (CYB12OK). Okara processing significantly affected nutrient digestibility: dry matter ADC was highest in CYR11OK (80%) and lowest in FOK (40%). The lowest protein digestibility was observed in CYR11OK (72%), and the highest in AOK (97%) and CYOK (91%), evidencing the effectiveness of the autoclave and the use of C. cardunculus proteases to increase okara protein bioavailability. The inclusion of up to 20% of AOK or CYOK did not affect fish growth, nutrient utilization, or whole body composition of Nile tilapia. The flesh quality (color, pH, water activity, cohesiveness, elasticity and resilience) was not affected by the dietary incorporation of AOK or CYOK. Fish fed with AOK diets stand out for their high density of muscle fibers, particularly in AOK20, which can explain their high muscle firmness and may result in further hypertrophic growth. Altogether, results suggest that hydrolyzed or autoclaved okara are valuable ingredients for Nile tilapia diets.

scholarly journals Ancient grains as novel dietary carbohydrate sources in canine diets

2021 ◽  
Author(s):  
Zachary T Traughber ◽  
Fei He ◽  
Jolene M Hoke ◽  
Gary M Davenport ◽  
Maria R C de Godoy
Keyword(s):  
Latin Square ◽  
Short Chain Fatty Acids ◽  
Fecal Microbiota ◽  
Nutrient Digestibility ◽  
Total Dietary Fiber ◽  
Carbohydrate Source ◽  
Beneficial Effects ◽  
Food Market ◽  
Dietary Treatments

Abstract Ancient grains are becoming an increasingly abundant carbohydrate source in the pet food market as a result of their popularity and novelty in the human market. Thus, it is imperative to evaluate the characteristics of these ingredients in vivo. Ten adult intact female beagles were used in a replicated 5x5 Latin square design. Five dietary treatments were evaluated containing either: rice (CON), amaranth (AM), white proso millet (WPM), quinoa (QU), or oat groats (OG). All diets were formulated to include 40% of the test grain and to be isonitrogenous, isocaloric, and nutritionally complete and balanced for adult dogs at maintenance. The objectives were (1) to evaluate the effects of the novel carbohydrate sources on total apparent total tract digestibility (ATTD), fecal microbiota, and fermentative end-product concentrations and (2) to evaluate the effects of novel carbohydrate sources on the post-prandial glycemic and insulinemic responses in healthy adult dogs. All diets were well-accepted by the dogs and fecal scores remained within ideal range for all treatments. In terms of ATTD, all diets were well-digested by the dogs; WPM had the highest digestibility of dry and organic matter in contrast with dogs fed the other treatments (P &lt; 0.05). Additionally, ATTD of total dietary fiber was highest for WPM (72.6%) in contrast with QU (63.5%) and CON (50.8%) but did not differ from AM (65.7%) and OG (66.6%). Dogs fed AM or OG had greater (P &lt; 0.05) fecal concentrations of total short-chain fatty acids, as well as propionate and butyrate concentrations, than CON. Ancient grain inclusion appears to beneficially shift fecal microbial populations, with increases in relative abundances of butyrogenic bacteria (i.e., members of the Lachnospiraceae family) observed for OG and reductions in Fusobacteriaceae for both AM and OG when compared with CON. Post-prandial glycemic and insulinemic responses did not differ among treatments. Together, these data suggest that ancient grains can be included up to 40% of the diet while eliciting beneficial effects on overall host health without detrimentally affecting nutrient digestibility.

scholarly journals Nutrient digestibility of multi-enzyme supplemented low-energy and AA diets for grower pigs1

2019 ◽  
Vol 97 (7) ◽  
pp. 2979-2988
Author(s):  
Jung Wook Lee ◽  
Robert Patterson ◽  
Anna Rogiewicz ◽  
Tofuko A Woyengo
Keyword(s):  
Latin Square ◽  
Nutrient Utilization ◽  
Positive Control ◽  
Negative Control ◽  
Linear Increase ◽  
Nutrient Digestibility ◽  
Low Energy ◽  
Partial Replacement ◽  
Complete Replacement ◽  
Corn Distillers

Abstract A study was conducted to determine effects of supplementing multi-enzyme on apparent ileal digestibility (AID) of energy and AA; and apparent total tract digestibility (ATTD) of energy for pigs fed low-energy and AA diets. Eight ileal-cannulated barrows (initial BW: 38.7 ± 2.75 kg) were fed four diets in a replicated 4 × 4 Latin square design to give 8 replicates per diet. Diets were positive control (PC) diet, negative control (NC) diet without or with multi-enzyme at 0.5 or 1.0 g/kg. The PC diet was formulated to meet or exceed NRC (2012) nutrient recommendations for grower pigs (25 to 50 kg), except for Ca and digestible P, which were lower than NRC (2012) recommendations by 0.13 and 0.17 percentage points, respectively, due to phytase supplementation at 1,000 FTU/kg. The NC diet was formulated to be lower in NE by 75 kcal/kg and standardized ileal digestible AA content by a mean of 3%. These reductions were achieved by partial replacement of corn and soybean meal (SBM) and complete replacement of soybean oil and monocalcium phosphate in PC diet with 25% corn distillers dried grains with solubles (DDGS) and 3.6% soybean hulls. Multi-enzyme at 1.0 g/kg supplied 1,900 U of xylanase, 300 U of β-glucanase, 1,300 U of cellulase, 11,500 U of amylase, 120 U of mannanase, 850 U of pectinase, 6,000 U of protease, and 700 U of invertase per kilogram of diet. The AID of GE, N, most AA, most component sugars of nonstarch polysaccharides (NSP) and P; ATTD of GE for PC diet was greater (P < 0.05) than those for NC diets. An increase in dietary level of multi-enzyme from 0 to 1.0 g/kg resulted in a linear increase (P < 0.05) in AID of Ile by 4.3%, and tended to linearly increase (P < 0.10) AID of Leu, Met, Phe, and Val by a mean of 3.4%. Increasing dietary multi-enzyme from 0 to 1.0 g/kg linearly increased (P < 0.05) AID of total NSP and P by 53.7% and 19.2%, respectively; ATTD of GE by 8.4% and DE and NE values by 8.8% and 8.2%, respectively; tended to linearly increase (P < 0.10) AID of GE by 8.1%. The NE values for NC diet with multi-enzyme at 1.0 g/kg tended to be greater (P < 0.10) than that for PC diet (2,337 vs. 2,222 kcal/kg of DM). In conclusion, multi-enzyme supplementation improved energy and nutrient digestibilities of a corn–SBM–corn DDGS-based diet, implying that the multi-enzyme fed in the current study can be used to enhance energy and nutrient utilization of low-energy AA diets for grower pigs.

Mikrobiom und gastrointestinale Erkrankungen

2019 ◽  
Vol 144 (14) ◽  
pp. 949-956
Author(s):  
Julian Siegel ◽  
Viola Andresen ◽  
Peter Layer
Keyword(s):  
Gastrointestinal Diseases ◽  
Intestinal Microbiome ◽  
Therapeutic Approaches ◽  
Fecal Microbiome ◽  
Gastrointestinale Erkrankungen ◽  
Microbiome Research ◽  
Long Time ◽  
Inflammatory Bowel ◽  
Complex Relationships ◽  
Pathogenic Agents

AbstractFor some years, microbiome research has become a thriving topic. The most diverse and hitherto poorly understood connections of interactions of the microbiome with the host body (human) seem to play an important role in the genesis of a wide variety of diseases. This article focuses on the importance of the microbiome in gastrointestinal diseases and presents therapeutic approaches.The intestinal microbiome will not be understood completely for a long time due to the highly complex relationships and variety of different microorganisms. The intestinal microbiome is critically involved in the development of diseases, especially in the development of inflammatory bowel disease. Helicobacter pylori infection is a major risk factor for gastric carcinoma. Esophageal microbiome alterations can cause inflammation and decrease the tone of the lower esophageal sphincter. Inflammation is a crucial pathway for tumorigenesis, it can be caused by the “normal” flora and by pathogenic agents. In the microbiome of the colon, interactions of bacteria, viruses and fungi have a very special status. Good oral/dental status protects against pancreatic carcinoma. Fecal microbiome transfer has become more important in the American guideline. Understanding the processes in the intestinal microbiome provides approaches to new therapies that are likely to exceed our current imagination.

Review: Anti-nutritional effects of phytic acid in diets for pigs and poultry – current knowledge and directions for future research

2013 ◽  
Vol 93 (1) ◽  
pp. 9-21 ◽  
Author(s):  
T. A. Woyengo ◽  
C. M. Nyachoti
Keyword(s):  
Gastrointestinal Tract ◽  
Phytic Acid ◽  
Current Knowledge ◽  
Nutrient Utilization ◽  
Nutrient Digestibility ◽  
Future Research ◽  
Nutrient Losses ◽  
Dietary Nutrients ◽  
Nutritional Effects ◽  
Insight Into

Woyengo, T. A. and Nyachoti, C. M. 2013. Review: Anti-nutritional effects of phytic acid in diets for pigs and poultry – current knowledge and directions for future research. Can. J. Anim. Sci. 93: 9–21. Plant feedstuffs contain phytic acid (PA), which is a storage form of phosphorus. Phytic acid is, however, poorly hydrolyzed by pigs and poultry, and it has a capacity to complex dietary nutrients, thereby reducing nutrient digestibility. Reduced nutrient digestibility by PA implies reduced efficiency of utilization of the nutrients and increased discharge of the unabsorbed nutrients to the environment. Phytic acid has also recently been shown to increase the endogenous nutrient losses (ENL) in pigs and poultry. Because the increased ENL in the gastrointestinal tract are associated with increased maintenance requirement for the lost nutrients and of energy spent on their secretion, an increase in ENL due to PA implies that there are other adverse effects of PA on nutrient utilization in addition to reducing nutrient digestibility. In this review, the effects of PA on performance of pigs and poultry, and on the digestibility and ENL in these animals are discussed in detail. Also, the mechanisms by which PA reduces nutrient digestibility and increases gastrointestinal ENL in pigs and poultry are discussed, and areas that need further research to gain more insight into these mechanisms are suggested.

Effect of supplementation of concentrate to sweet sorghum bagasse with leaf residue silage on nutrient utilization and nitrogen balance in native sheep

2016 ◽  
Author(s):  
B. Vidya ◽  
Y. Raman Reddy ◽  
D. Srinivasa Rao ◽  
V. Ravinder Reddy ◽  
N. Nalini Kumari ◽  
...  
Keyword(s):  
Nitrogen Balance ◽  
Sweet Sorghum ◽  
Crude Protein ◽  
Nutrient Utilization ◽  
Nutrient Digestibility ◽  
Sweet Sorghum Bagasse ◽  
Significant Difference ◽  
Sorghum Bagasse ◽  
Leaf Residue ◽  
Concentrate Mixture

The present study was carried out to evaluate the effect of supplementation of concentrate at different levels to sweet sorghum bagasse leaf residue (SSBLR) silage on nutrient utilization in growing lambs in a metabolism trial conducted after 120days of feeding. Sixteen growing Nellore ram lambs (18.3±0.8) were randomly allotted to 4 dietary groups (4 in each group) and were inducted into metabolic cages five days prior to collection for acclimatization followed by a seven day collection period. The four dietary groups were SSBLR silage fed ad libitum and supplemented with concentrate mixture (CP 17% and ME 2.53 Mcal/kg DM) at 0.0 (R-I), 0.75 (R-II), 1.0 (R-III) and 1.25 (R-IV) per cent of their live Weight. Significantly (P<0.01) higher total DMI (g/kg w0.75) was noticed in concentrate supplemented groups. The organic matter, crude protein and nitrogen free extract digestibility was significantly (P<0.05) higher in lambs fed R-IV ration than those fed R-I ration. No significant difference in the digestibility of dry matter, ether extract and cell wall constituents was observed among the rations. The digestible crude protein (%) (P<0.01) and metabolizable energy (Mcal/kg) values increased (P>0.0) with increase in level of concentrate in the diet. Negative and lower (P<0.01) nitrogen balance (g/d) was noticed in R-I ration. The N balance linearly increased with increase in level of concentrate supplementation. The study indicated feeding of sole SSBLR silage couldn't meet the nutrient requirements and Supplementation of concentrate mixture (0.75-1.25%) to SSBLR silage improved the nutrient digestibility and nitrogen balance in growing lambs.

Effect of sex on growth performance, nutrient utilizationand carcass characteristics in cross bred pigs

2017 ◽  
Author(s):  
Gowher Gull Sheikh ◽  
R.P.S. Baghel ◽  
Sunil Nayak ◽  
Aijaz Ahmad Ganie
Keyword(s):  
Growth Performance ◽  
Nutrient Utilization ◽  
Nutrient Digestibility ◽  
Daily Weight Gain ◽  
Feed Conversion ◽  
Large White ◽  
Growing Period ◽  
Significant Difference ◽  
Nonsignificant Difference ◽  
Gender Groups

A study was carried on 24 piglets (Large White York Shire × Desi) of an average initial weight of 20 kg of three gender groups, barrows, boars and gilts consisted of eight pigs each, of comparable body weight fattened from 20-48 kilograms (growing) to 49-96 kilograms (finishing) to see the effect of gender on growth performance, nutrient utilization and carcass traits. When comparing feed intake at the growing stage, gilts consumed significantly more (P less than 0.05) feed than boars and barrows. There was no significant difference in average daily weight gain among barrows, boars and gilts in growing and finishing stage. Gilts had significantly higher (P less than 0.05) feed conversion ratio than boars in growing period and barrows in finishing period. Nutrient digestibility was similar except crude protein among groups. Fasting weight and weight after bleeding was significantly lower (P less than 0.05) in gilts with nonsignificant difference between barrows and boars. Sex has no significant effect on evisceration weight, dressing percentage and carcass length in pigs. Carcass backfat of barrows was significantly thicker (P less than 0.05) than that of boars and gilts, however, the loin eye area was significantly larger (P less than 0.05) in gilts than boars and barrows. There were no significant differences among groups concerning the proportions of ham, under cut, bacon and rib chop however, shoulder and loin percentages were higher (P less than 0.05) in gilts than boars and barrows.

Performance and nutrient utilization of growing pigs given an expanded and pelleted diet

1998 ◽  
Vol 66 (1) ◽  
pp. 225-230 ◽  
Author(s):  
J. Vande Ginste ◽  
R. De Schrijver
Keyword(s):  
Control Diet ◽  
Protein Solubility ◽  
Live Weight ◽  
Experimental Period ◽  
Nutrient Utilization ◽  
Growing Pigs ◽  
Adaptation Period ◽  
Crude Fibre ◽  
Pelleted Diet

AbstractA grower diet containing barley, wheat and soya-bean meal was expanded at 110°C and subsequently pelleted at 80°C. This processing was evaluated in laboratory tests as well as in digestibility experiments involving 12 barrows with an average initial live weight of 40 kg. The unprocessed control diet was offered as a meal. Each diet was offered ad libitum to six pigs during a 5-week period. The 1st week was an adaptation period and measurements were not carried out. Each pig was used in two 5-day digestibility trials which were performed in weeks 2 and 4. Neither food intake, weight gain nor food: gain ratio during the whole 4-week experimental period, nor apparent faecal digestibility and apparent retention of protein were significantly affected (P > 0·05) by expanding and pelleting the diet. Processing caused an increase in the in vitro protein solubility (P < 0·05) and reduced the dietary contents of free lysine and methionine (P < 0·05) while the contents of available lysine and free threonine and tryptophan were not significantly changed (P > 0·05). Apparent faecal digestibility of crude fibre increased substantially (P < 0·05) when the diet was processed, resulting in significantly lower production of faecal mass (P < 0·05) as well as lowerfaecal moisture content (P < 0·05). These phenomena were parallelled by a smaller water consumption (P < 0·05). Apparent digestibility and retention of phosphorus and calcium were diminished (P < 0·05) when the diet was expanded and pelleted.

scholarly journals Growth performance and nutrient digestibility of broiler chickens fed diets substituted for maize with two varieties of cassava tuber

2020 ◽  
Vol 46 (2) ◽  
pp. 128-136
Author(s):  
A. O. Obadimu ◽  
O. O. Oluwatosin ◽  
I. O. Sanni ◽  
A. O. Lala
Keyword(s):  
Weight Gain ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Control Diet ◽  
Nutrient Utilization ◽  
Nutrient Digestibility ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Feed Conversion ◽  
Final Weight

A 56 –day study was conducted to determine the growth performance and nutrient utilization of broiler chickens fed with two varieties (white and yellow) of cassava grit based diets. A total of 240 one day- old unsexed Arbor acre broiler chicks were assigned to eighty dietary treatments in a 2 x 4 factorial arrangement using completely randomized design. Diets were formulated to include two varieties of cassava, TME 419 (white) and TMS 01 1368 (yellow) at four inclusion levels (0, 25, 50 and 75%). Each treatment consisted of 30 birds and three replicates of 10 birds each. Data was collected on growth performance and nutrient digestibility at the end of the starter (4th week) and finisher (8th week) phases. At the starter phase, birds fed 50% white cassava grits diet had the highest (P < 0.05) final weight and weight gain (730.00 and 693.67g/bird). At the finisher phase, birds fed 50% yellow cassava grits diet had the highest (P < 0.05) final weight and weight gain (2235.60 and 1454.00g/bird), respectively while the least final weight and weight gain values (2008.80 and 1267.80g/birds) were obtained in birds fed the control diet. At the starter phase, birds fed 75% white cassava grits diet had the best (P < 0.05) feed conversion ratio of 2.18, while birds fed 25% yellow cassava grits diet as well as those fed the control diet had the least (P < 0.05) feed conversion ratio of 2.62 and 2.51 respectively. Birds fed 50% yellow as well as those fed 75% yellow cassava grits diets had the highest dry matter and crude fibre digestibility at the starter and finisher phases, respectively. In conclusion the substitution of maize with white cassava grits up to 50% level improved growth performance at the starter phase. However, either white or yellow cassava can be substituted for maize up to 75 % at the finishing phase.

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