scholarly journals Porcine Circovirus Type 3 in Pig Farms Experiencing Diarrhea in Jiangxi, China: Prevalence, Genome Sequence and Pathogenicity

Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2324
Author(s):  
Fanfan Zhang ◽  
Weifeng Yuan ◽  
Zhiquan Li ◽  
Yuhan Zhang ◽  
Xiuxiu Zeng ◽  
...  
Keyword(s):  
Complete Genome ◽  
Amino Acid Level ◽  
Clinical Signs ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Weaned Piglets ◽  
Gross Lesions ◽  
Genome Level ◽  
Genome Analyses ◽  
Type 3

Porcine circovirus 3 (PCV3) infections have been reported in different clinical presentations. However, the prevalence and pathogenicity of PCV3 associated with diarrhea in piglets have been limited. Herein, we present an investigation and genome analyses of PCV3 in piglets experiencing diarrhea, and observed clinical signs, gross lesions, and histological changes in pigs negative for all known pathogens associated with diarrhea but positive for PCV3 alone. Among the feces (n = 141) tested, 16.31% (23/141) were positive for PCV3. Of which, 27.28% (15/55) and 14.29% (5/35) were present in diarrheal samples from suckling and weaned piglets, respectively. Moderate to severe atrophic villi was confined in duodenum, jejunum, and ileum, and significantly decreased average heights of villi, and the depths of crypt were observed in PCV3-infected piglets. The complete genome of a representative strain of PCV3, designated as JX/CH/2018, was determined. Multialignment analysis indicated that JX/CH/2018 had 97.7–99.7% nucleotide identity at the complete genome level, and 97.2–100% at the amino acid level of the capsid protein when compared with reference PCV3 strains. Phylogenetic analysis showed that the PCV3 strain identified in this study belonged to PCV3a lineage. The present study demonstrated that PCV3 is a common virus in diarrheal suckling and weaned piglets.

First report of wild boar susceptibility to Porcine circovirus type 3: High prevalence in the Colli Euganei Regional Park (Italy) in the absence of clinical signs

2018 ◽  
Vol 65 (4) ◽  
pp. 957-962 ◽  
Author(s):  
Giovanni Franzo ◽  
Claudia Maria Tucciarone ◽  
Michele Drigo ◽  
Mattia Cecchinato ◽  
Marco Martini ◽  
...  
Keyword(s):  
Wild Boar ◽  
Clinical Signs ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
First Report ◽  
High Prevalence ◽  
Type 3

scholarly journals First detection of porcine circovirus type 3 in Colombia and the complete genome sequence demonstrates the circulation of PCV 3a1 and PCV 3a2

2019 ◽  
Vol 5 (2) ◽  
pp. 182-188 ◽  
Author(s):  
Diana S. Vargas‐Bermudez ◽  
Fabricio S. Campos ◽  
Laura Bonil ◽  
Darío Mogollon ◽  
Jairo Jaime
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Type 3

scholarly journals Induction of Porcine Dermatitis and Nephropathy Syndrome in Piglets by Infection with Porcine Circovirus Type 3

2018 ◽  
Vol 93 (4) ◽  
Author(s):  
Haijun Jiang ◽  
Dan Wang ◽  
Jing Wang ◽  
Shanshan Zhu ◽  
Ruiping She ◽  
...  
Keyword(s):  
Specific Antigen ◽  
Clinical Signs ◽  
Keyhole Limpet Hemocyanin ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Significant Information ◽  
Pathological Lesions ◽  
Cytokines And Chemokines ◽  
Specific Pathogen ◽  
Type 3

ABSTRACT Porcine circovirus type 3 (PCV3) is an emerging porcine circovirus that has been associated with porcine dermatitis and nephropathy syndrome (PDNS)-like clinical signs, reproductive failure, cardiac pathologies, and multisystemic inflammation in piglets and sows. Many aspects of PCV3 infection biology and pathogenesis, however, remain unknown. Here, we used a PCV3 virus stock from the rescue of an infectious PCV3 DNA clone to intranasally inoculate 4- and 8-week-old specific-pathogen-free piglets for evaluation of PCV3 pathogenesis. For 4-week-old piglets, typical clinical signs resembling those of PDNS-like disease were observed when piglets were inoculated with PCV3 alone or PCV3 combined with immunostimulation by keyhole limpet hemocyanin, with a mortality of 40% (2/5) for both types of inoculated piglets during a 28-day observation period postinoculation. Both types of inoculated piglets showed similar progressive increases in viral loads in the sera and had seroconverted to PCV3 capsid antibody after inoculation. Pathological lesions and PCV3-specific antigen were detected in various tissues and organs, including the lung, heart, kidney, lymph nodes, spleen, liver, and small intestine, in both types of inoculated piglets. The levels of proinflammatory cytokines and chemokines, including interleukin 1 beta (IL-1β), IL-6, IL-23α, gamma interferon (IFN-γ), tumor necrosis factor alpha (TNF-α), and chemokine ligand 5 (CCL5), were significantly upregulated in both groups of inoculated piglets. Eight-week-old piglets also exhibited a similar PDNS-like disease but without death after PCV3 inoculation, as evidenced by pathological lesions and PCV3 antigen in various tissues and organs. These results show for the first time successful reproduction of PDNS-like disease by PCV3 infection and further provide significant information regarding the pathogenesis of PCV3 in piglets. IMPORTANCE Porcine circovirus type 3 (PCV3), an emerging porcine circovirus, is considered the cause of porcine dermatitis and nephropathy syndrome (PDNS)-like clinical signs and other systemic diseases in piglets and sows. To evaluate the pathogenesis of PCV3 infection in vivo, we used a PCV3 virus stock from the rescue of an infectious PCV3 DNA clone to intranasally inoculate 4- and 8-week-old specific-pathogen-free piglets and demonstrated successful reproduction of PDNS-like disease in animals that were inoculated with PCV3 alone or PCV3 combined with immunostimulation by keyhole limpet hemocyanin. Both 4- and 8-week-old PCV3-inoculated piglets showed similar increases in viral loads in the sera and had seroconverted to PCV3 capsid antibody. Pathological lesions and PCV3-specific antigen were detected in various tissues and organs, while numerous proinflammatory cytokines and chemokines in the sera were significantly upregulated after PCV3 inoculation. These results will provide significant information regarding the pathogenesis of PCV3 in piglets.

scholarly journals Complete Genome Sequence of a Novel Porcine Circovirus Type 3 Strain, PCV3/CN/Hubei-618/2016, Isolated from China

2017 ◽  
Vol 5 (15) ◽  
Author(s):  
Shengxian Fan ◽  
Xugang Ku ◽  
Fangzhou Chen ◽  
Ying Wang ◽  
Xuexiang Yu ◽  
...  
Keyword(s):  
United States ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
The United States ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Genetic Characteristics ◽  
Type 3

ABSTRACT In October 2016, porcine circovirus type 3 (PCV3) was identified as a pathogen agent for pigs in the United States. Here, we report the genome sequence of a Chinese PCV3 strain, PCV3/CN/Hubei-618/2016. This will help us better understand the epidemiology and genetic characteristics of PCV3.

scholarly journals High repetitive arginine in the anterior of PCV3 capsid protein is a severe obstacle for its expression in E. coli

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Bing Yan Liu ◽  
Bin Gao ◽  
Meng Zhi Liu ◽  
Ting Ting Zhang ◽  
Bao Shan Liu ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Vaccine Candidate ◽  
Clinical Signs ◽  
Porcine Circovirus Type ◽  
Reproductive Failure ◽  
Porcine Circovirus ◽  
Effective Vaccine ◽  
E Coli ◽  
Efficient Expression ◽  
Type 3

AbstractPorcine circovirus type 3 (PCV3) is a novel circovirus identified in sows with PDNS-like clinical signs and reproductive failure. The capsid protein (CAP) of PCV3 is expected to be an effective vaccine candidate. Here, we expressed the original capsid protein, truncated capsid protein without anterior highly repetitive arginine (ΔCAP) and their codon-optimized counterparts in E. coli. These results showed that lots of repeated arginine could severely lower the expression of PCV3 capsid protein in E. coli. At the same time, the recombined truncated PCV3 capsid protein forms typic virions. The efficient expression of capsid protein is expected to serve the development of PCV3 vaccines and other studies of PCV3 capsid protein.

Effect of neutraceutical supplementation on the growth performance and antioxidant activity in liver and plasma of weaned piglets

2004 ◽  
Vol 31 ◽  
pp. 185-192
Author(s):  
J.H. Guy ◽  
S. Arkle ◽  
D. Mantle ◽  
C.J. Seal ◽  
P.J. Blanchard ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Immune System ◽  
Growth Performance ◽  
Clinical Signs ◽  
Natural Antioxidants ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Weaned Piglets ◽  
Immune Stress ◽  
Wasting Syndrome

AbstractPost–Weaning Multisystemic Wasting Syndrome (PMWS) can result in mortality levels of up to 80% in weaned piglets, and Porcine Circovirus type 2 (PCV2) has been identified as one of the primary causative agents. Neutraceutical products, based on plant and herbal extracts and containing high levels of natural antioxidants may be beneficial in reducing stress on the piglets’ immune system by elevating blood antioxidant levels, and assisting with the piglets defence against PMWS. The aim of this experiment was to assess the effect of supplementation with neutraceutical products on the growth performance and antioxidant activity in liver and plasma of piglets. It was undertaken on a farm positive for PCV2, but where pigs were not showing clinical signs of PMWS. Groups of newly weaned pigs were offered one of three diets ad libitum for a period of 26 days, standard creep and starter/link control rations, or one of two experimental rations (including Neutraceutical–mix, N, or Lycopene, L), chosen for their potential to boost antioxidant activity. Growth performance and feed intake were monitored, and samples of blood and liver collected at slaughter (54 days of age) and analysed for antioxidant activity. Although L pigs ate significantly less feed during the creep period (P<0.05), there were relatively few performance differences between treatments. However total antioxidant activity in liver samples was significantly higher for the N treatment (P<0.001) and activity of plasma superoxide dismutase was significantly higher in the L group. In conclusion, growth performance of weaned piglets following the addition of Neutraceutical supplements was similar to control groups, and there were significant increases in antioxidant activity of these animals. On the basis that increased levels of circulating blood antioxidants reduce stress on the piglets’ immune system, the results of the current study suggest that these supplements may be of benefit to piglets under immune stress, such as in clinical cases of PMWS.

scholarly journals Complete Genome Sequences of Two Type 3 Porcine Circoviruses, WB17KW and WB20GG, Isolated from Korean Wild Boar

2021 ◽  
Vol 10 (6) ◽  
Author(s):  
Sok Song ◽  
Kyu-Nam Park ◽  
SeEun Choe ◽  
Ra Mi Cha ◽  
Jihye Shin ◽  
...  
Keyword(s):  
South Korea ◽  
Wild Boar ◽  
Complete Genome ◽  
Genetic Relatedness ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus ◽  
Genome Sequences ◽  
Domestic Pigs ◽  
Porcine Circoviruses ◽  
Type 3

ABSTRACT Porcine circovirus type 3 (PCV3) in domestic pigs was first reported in South Korea in 2017. Here, we report the first complete genome sequences of two PCV3 strains isolated from Korean wild boar, which enhance our understanding about the genetic relatedness of PCV3 in domestic pigs and wild boar.

scholarly journals Full-Length Genome Sequences of Two Chinese Porcine Circovirus Type 3 Strains, NWHEB21 and NWHUN2

2018 ◽  
Vol 6 (7) ◽  
Author(s):  
Can Liu ◽  
Shasha Chen ◽  
Fanwei Meng ◽  
Rui Chen ◽  
Zhigang Zhang ◽  
...  
Keyword(s):  
Complete Genome ◽  
Porcine Circovirus Type ◽  
Full Length ◽  
Porcine Circovirus ◽  
Genome Sequences ◽  
Porcine Circovirus 3 ◽  
Type 3 ◽  
Full Length Genome ◽  
Brain Tissues

ABSTRACT Two porcine circovirus 3 (PCV3) strains, named NWHEB21 and NWHUN2, were identified in heart and brain tissues of aborted piglets. Their complete genome sequences were sequenced and analyzed to further characterize PCV3 in China and worldwide.

scholarly journals Diagnosis of post-weaning multisystemic wasting syndrome in pigs in Brazil caused by porcine circovirus type 2

2003 ◽  
Vol 55 (5) ◽  
pp. 522-527 ◽  
Author(s):  
J.R. Ciacci-Zanella ◽  
N. Morés
Keyword(s):  
Clinical Signs ◽  
Rflp Analysis ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Nested Polymerase Chain Reaction ◽  
Wasting Syndrome ◽  
Thymus Atrophy ◽  
Granulomatous Lymphadenitis

This report describes the first preliminary characterization of porcine circovirus type 2 (PCV2) isolates from pigs affected with post-weaning multisystemic wasting syndrome (PMWS) in Brazil. Diseased pigs were examined at necropsy and by histopathology. Macroscopic and microscopic analyses revealed lesions reported to be typical of PMWS, which included, respectively, emaciation, enlargement of lymph nodes, thymus atrophy and interstitial pneumonia, and granulomatous lymphadenitis with syncytial cells, among others. Using nested polymerase chain reaction (PCR) or imunoperoxidase it was possible to detected DNA or antigen of PCV2, respectively. The PCR' s amplified fragment could be differentiated from PCV1 and PCV2 from one another by restriction fragment length polymorphism (RFLP) analysis. PCV2 DNA was detected in 70% (14/20) of samples of pigs with clinical signs and lesions associated with PMWS. This study shows that PCV2 is associated with lesions and symptoms indicative of PMWS in pigs. It is also shown that the Brazilian PCV2 isolates may have variation in their genome.

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