scholarly journals Silage of Prickly Pears (Opuntia spp.) Juice By-Products

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1716
Author(s):  
Alessandro Vastolo ◽  
Serena Calabrò ◽  
Monica Isabella Cutrignelli ◽  
Girolamo Raso ◽  
Massimo Todaro
Keyword(s):  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Sugar Content ◽  
Nitrogen Concentration ◽  
Ammonia Nitrogen ◽  
Prickly Pear ◽  
Human Consumption ◽  
Nutritional Characteristics ◽  
Prickly Pears ◽  
By Products

Cactus pear cladodes are used as forage in the most arid regions. In Italy, the human consumption of prickly pear fruits and juice is gradually increasing for their numerous health benefits. In manufacturing plants that produce prickly pear juice, several by-products (prickly pear by-products PPB) are obtained. Despite their interesting nutritional characteristics, PPB are not very usable because of their poor shelf-life which is related to their high moisture and sugar content. The aim of this study was to verify the efficacy of ensilage to preserve PPB and to compare different inclusion levels (0, 5, and 10% as fed) of wheat straw. For each treatment, four under vacuum micro-silos were prepared and, after 35 days of storage, the state of preservation was evaluated. Subsequently, the aliquots were analyzed for chemical composition and incubated with bovine rumen fluid to evaluate the fermentation kinetics. The PPB 5% of straw showed significant lower pH and ammonia nitrogen concentration, indicating a better preservation process. Moreover, PPB 5% of straw showed better nutritional parameters (higher crude protein and lower Neutral Detergent Fibre) and fermentation characteristics (higher degradability and VFA volatile fatty acids production) when compared with the other PPB silages. Ensilage with straw represents a suitable storage technique to preserve the nutritional characteristics of PPB.

Optimizing volatile fatty acid production in partial acidogenesis of swine wastewater

2004 ◽  
Vol 50 (8) ◽  
pp. 169-176 ◽  
Author(s):  
K. Yang ◽  
C. Oh ◽  
S. Hwang
Keyword(s):  
Propionic Acid ◽  
Acid Production ◽  
Volatile Fatty Acids ◽  
Nitrogen Concentration ◽  
Ammonia Nitrogen ◽  
Swine Wastewater ◽  
Fatty Acid Production ◽  
Stirred Tank Reactors ◽  
Physiological Conditions ◽  
Propionic Acid Production

This research has been conducted based on the fact that acetic and butyric acids are favorable substrates for methanogens, and that a low level of propionic acid production during acidogenesis minimizes the inhibition effect on methanogenic growth. Raw swine wastewater was pretreated with ammonia stripping to enhance acidogenesis. The ammonia nitrogen concentration of less than 1.2 g/L did not significantly affect the biochemical acidogenic potential of swine wastewater. For acidogenesis of swine wastewater, a et of experiments were carried out to produce short chain volatile fatty acids (VFA) in laboratory-scale continuously stirred tank reactors. The production of acetic, propionic, and butyric acids associated with simultaneous changes in hydraulic retention time (HRT) and temperature was investigated. Response surface methodology was successfully applied to approximate the responses of the VFA productions. The optimum physiological conditions where the maximum acetic and butyric acids production occurred were 2.4 days HRT at 34°C and 2.1 days HRT at 35°C, respectively. The propionic acid production linearly increased as both HRT and temperature increased.

The effect of lipid sources on intake, rumen fermentation parameters and microbial protein synthesis in Nellore steers supplemented with glycerol

2014 ◽  
Vol 54 (10) ◽  
pp. 1871 ◽  
Author(s):  
Arturo Samuel Gomez Insuasti ◽  
Yury Tatiana Granja Salcedo ◽  
Pablo de Souza Castagnino ◽  
Bruno Ramalho Vieira ◽  
Euclides Braga Malheiros ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Ether Extract ◽  
Microbial Protein ◽  
Molar Proportion ◽  
Fermentation Parameters

The effects of glycerol with fat sources as a feed alternative were investigated in the ration for Nellore steers (Bos indicus). Eight cannulated steers at 30 months of age with initial bodyweight of 554 ± 36.0 kg were used in a double 4 by 4 Latin square design with four consecutive 16-day periods. Treatments were three different diets with lipid sources (soybean, soybean oil and calcium salts of fatty acids) and one diet control without lipid sources. All diets formulated contained 10% crude glycerol and 5% ether extract with a forage:concentrate ratio of 35:65; corn silage was used as forage. The ruminal liquid was sampled for 24 h and ruminal fermentations were monitored by measuring pH, concentrations of ammonia nitrogenand volatile fatty acids in rumen fluid. Urine samples were obtained from the total collection for 24 h for estimation of rumen microbial protein supply using urinary purine derivatives. Our results showed that the use of lipid sources combined with glycerol did not induce significant changes in rumen pH, acetate molar proportion, ruminal microbial protein or dry matter intake. Although the acetate molar proportion was kept constant within normal parameters, the propionate molar proportion was increased by the diet containing lipid sources. Moreover, we found that there was a negative effect of lipid sources on crude protein and neutral detergent fibre corrected for ash and protein intake, and one positive effect on ether extract intake. We believe that association between fat and glycerol may affect rumen fermentation parameters through reducing fibre intake and increasing propionate production and ammonia nitrogen.

Use of Near Infrared Spectroscopy (MRS) to Monitor Changes in the Composition of Rumen Fluid

1994 ◽  
Vol 1994 ◽  
pp. 109-109
Author(s):  
R. Sanderson ◽  
S.J. Lister ◽  
M.S. Dhanoa
Keyword(s):  
Volatile Fatty Acids ◽  
Near Infrared ◽  
Rapid Determination ◽  
Rumen Fluid ◽  
Packed Column ◽  
Rapeseed Meal ◽  
Ammonia Nitrogen ◽  
Fluid Composition ◽  
Lactating Cows ◽  
Glutamic Dehydrogenase

To obtain more information about rumen fluid composition, levels of different components are determined by a range of techniques which are often slow and cumbersome. This study was established to develop calibration equations for the rapid determination of the composition of rumen fluid with a view to monitoring changes in composition with time.Forty-three strained rumen fluid samples, taken throughout a 7 h period from three lactating cows offered silage either alone or with fish meal or rapeseed meal/urea twice daily, were used. Immediately upon withdrawal from the rumen each sample was analysed for pH, then acidified using concentrated sulphuric acid before storing at -18°C. Following centrifugation at 11600 G concentrations of volatile fatty acids and ammonia-nitrogen were determined in the supernatant by packed column gas chromatography and enzymatically using glutamic dehydrogenase respectively.

scholarly journals Multiple-factor associative effects of peanut shell combined with alfalfa and concentrate determined by in vitro gas production method

2019 ◽  
Vol 64 (No. 8) ◽  
pp. 352-360
Author(s):  
Jiu Yuan ◽  
Xinjie Wan
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Production Method ◽  
Ammonia Nitrogen ◽  
Gas Production ◽  
Peanut Shell ◽  
Dry Matter Digestibility ◽  
In Vitro Gas Production

The associative effects (AE) between concentrate (C), peanut shell (P) and alfalfa (A) were investigated by means of an automated gas production (GP) system. The C, P and A were incubated alone or as 40 : 60 : 0, 40 : 45 : 15, 40 : 30 : 30, 40 : 15 : 45, 40 : 0 : 60 and 30 : 70 : 0, 30 : 55 : 15, 30 : 40 : 30, 30 : 25 : 45, 30 : 10 : 60, 30 : 0 : 70 mixtures where the C : roughage (R) ratios were 40 : 60 and 30 : 70. Samples (0.2000 ± 0.0010 g) of single feeds or mixtures were incubated for 96 h in individual bottles (100 ml) with 30 ml of buffered rumen fluid. GP parameters were analysed using a single exponential equation. After incubation, the residues were used to determine pH, dry matter digestibility (DMD), organic matter digestibility (OMD), volatile fatty acids (VFA) and ammonia nitrogen (NH<sub>3</sub>-N) of the incubation fluid, and their single factor AE indices (SFAEI) and multiple-factors AE indices (MFAEI) were determined. The results showed that group of 30 peanut shell had higher SFAEI of GP<sub>48 h</sub>, DMD, OMD and total volatile fatty acids (p &lt; 0.05) and MFAEI (p &lt; 0.05) than groups 60, 45 and 0 when C : R was 40 : 60. The group of 10 peanut shell showed higher SFAEI of GP<sub>48 h</sub>, DMD and OMD (p &lt; 0.05) than groups 70, 55 and 40 and MFAEI (p &lt; 0.01) when C : R was 30 : 70. It is concluded that optimal SFAEI and MFAEI were obtained when the C : P : A ratios were 40 : 30 : 30 and 30 : 10 : 60.

scholarly journals Effect of Dietary Fat on the Metabolism of Energy and Nitrogen, Serum Parameters, Rumen Fermentation, and Microbiota in twin Hu Male Lambs

2018 ◽  
Author(s):  
Wenjuan Li ◽  
Hui Tao ◽  
Naifeng Zhang ◽  
Tao Ma ◽  
Kaidong Deng ◽  
...  
Keyword(s):  
Dietary Fat ◽  
High Fat Diet ◽  
Volatile Fatty Acids ◽  
High Throughput Sequencing ◽  
Rumen Fluid ◽  
Ammonia Nitrogen ◽  
Energy Utilization ◽  
Sequencing Analysis ◽  
Ruminal Fermentation ◽  
High Fat

AbstractBackgroundFat is the main substance that provides energy to animals. However, the use of fat in twin Hu lambs has not been investigated. Thirty pairs of male twin lambs were examined to investigate the effects of dietary fat on the metabolism of energy and nitrogen, ruminal fermentation, and microbial communities. The twins are randomly allotted to two groups (high fat: HF, normal fat: NF). Two diets of equal protein and different fat levels. The metabolism test was made at 50-60 days of age. Nine pairs of twin lambs are slaughtered randomly, and the rumen fluid is collected at 60 days of age.ResultsThe initial body weight (BW) in the HF group did not differ from that of NF group (P > 0.05), but the final BW was tended to higher than that of NF group (0.05 < P < 0.1). The digestive energy (DE), metabolism energy (ME), DE/ME in the HF group tend to be higher than those in the NF group (0.05 < P < 0.1). Ruminal ammonia nitrogen (NH3-N) and the proportion of total volatile fatty acids (TVFA) are higher than that in the NF group (P < 0.05). A high throughput sequencing analysis reveals that there were no differences between the two groups in terms of the richness estimates and diversity indices (P > 0.05). The Proteobacteria and Fibrobacteres phyla were higher than that in NF group (P<0.05).ConclusionsThis study demonstrated that high fat diet before weaning can affect the abundance of several groups of rumen bacteria in rumen, such as significantly increasing phyla Proteobacteria and Fibrobacteres, and genera of Succinivibrio, Alloprevotella, and Saccharofermentans, but significantly decreasing genera of Clostridium IV, Dialister, Roseburia, and Butyrivibrio. And high fat diet improved the performance of lambs at weight gain, energy utilization, and had effect on VFA composition but no effects on serum enzymes and serum hormone.

A comparison of various methods of feeding supplements of urea, sulfur and molasses to weaner heifers during the dry season in northern Queensland

1991 ◽  
Vol 31 (2) ◽  
pp. 153 ◽  
Author(s):  
SR McLennan ◽  
DJ Hirst ◽  
RK Shepherd ◽  
KR McGuigan
Keyword(s):  
Rumen Fluid ◽  
Nitrogen Concentration ◽  
Ammonia Nitrogen ◽  
Control Group ◽  
Dry Period ◽  
The Us ◽  
Native Pasture ◽  
Nitrogen Concentrations ◽  
Dry Seasons ◽  
Group 2

Over 3 dry seasons (1984-86), delivery systems of urea supplements were evaluated on weaner heifers grazing native pasture. Daily treatments per heifer were urea-ammonium sulfate (US) (28g + 6g), via the drinking water; urea-molasses (UM) (30g + 250g), via roller drum lick feeders; UM2 (60g + 1000g), via open troughs. Rainfall recordings were well above average in all dry seasons. The only liveweight response to supplementation occurred in 1986, during a dry period from June to October. During this period the liveweight performance of the US group was improved relative to the control group (+7 v. -57 g/heifer.day; P<0.05). The response of the UM2 group (+82 g/heifer.day) exceeded (P<0.05) that of all other groups. There was no response to the UM supplement (-51 g/heifer.day). Over the combined dry and following wet seasons the UM2 group grew faster (P<0.05) than all other groups. Ammonia-nitrogen concentrations in the rumen fluid of heifers, measured in the 1984 and 1986 dry seasons, were very low for the control group (7-54 mg/L rumen fluid) but were much higher in the US group 2 h after drinking (108-152 mg/L). At 18 h after drinking, the ammonia-nitrogen concentration had declined markedly in the US group (30-54 mg/L) but, in 2 of 3 observations, was still higher than that of the controls (significantly in 1 case; P<0.05).

scholarly journals Effects of Thymol Supplementation on Goat Rumen Fermentation and Rumen Microbiota In Vitro

2020 ◽  
Vol 8 (8) ◽  
pp. 1160 ◽  
Author(s):  
Jiangkun Yu ◽  
Liyuan Cai ◽  
Jiacai Zhang ◽  
Ao Yang ◽  
Yanan Wang ◽  
...  
Keyword(s):  
Methane Production ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Optimal Dose ◽  
Illumina Miseq ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Rumen Microbiota

This study was performed to explore the predominant responses of rumen microbiota with thymol supplementation as well as effective dose of thymol on rumen fermentation. Thymol at different concentrations, i.e., 0, 100 mg/L, 200 mg/L, and 400 mg/L (four groups × five replications) was applied for 24 h of fermentation in a rumen fluid incubation system. Illumina MiSeq sequencing was applied to investigate the ruminal microbes in addition to the examination of rumen fermentation. Thymol doses reached 200 mg/L and significantly decreased (p < 0.05) total gas production (TGP) and methane production; the production of total volatile fatty acids (VFA), propionate, and ammonia nitrogen, and the digestibility of dry matter and organic matter were apparently decreased (p < 0.05) when the thymol dose reached 400 mg/L. A thymol dose of 200 mg/L significantly affected (p < 0.05) the relative abundance of 14 genera of bacteria, three species of archaea, and two genera of protozoa. Network analysis showed that bacteria, archaea, and protozoa significantly correlated with methane production and VFA production. This study indicates an optimal dose of thymol at 200 mg/L to facilitate rumen fermentation, the critical roles of bacteria in rumen fermentation, and their interactions with the archaea and protozoa.

scholarly journals Effects of dietary protein level on intake, digestibility, and energy expenditure in dairy heifers

2020 ◽  
Vol 41 (6supl2) ◽  
pp. 3211-3224
Author(s):  
Laylles Costa Araújo ◽  
◽  
Eriton Egidio Lisboa Valente ◽  
Valdir da Trindade Filipini ◽  
Silvana Teixeira Carvalho ◽  
...  
Keyword(s):  
Energy Expenditure ◽  
Dietary Protein ◽  
Protein Level ◽  
Rumen Fluid ◽  
Nitrogen Concentration ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Neutral Detergent Fiber ◽  
Nitrogen Excretion ◽  
Dietary Protein Level

Balancing diets for protein affects both protein and energy use efficiency in cattle. This study aimed to evaluate the effects of dietary protein level on intake, digestibility and energy expenditure in Holstein heifers. Four Holstein heifers with a mean BW of 266.5 ± 10.7 kg were distributed in a 4 x 4 balanced Latin square design. Each period was comprised of 14 d for adaptation and 6 d for samplings. The heifers were fed diets with 9, 12, 15 and 18% of crude protein (CP). Total feces and urine were collected. Rumen fluid was collected at 2, 4, 6 and 8 h after feeding. The energy expenditure was evaluated at 6 a.m., 12 a.m., 6 p.m. and 12 p.m. by a closed-circuit mask technique. The intake of dry matter (DM), organic matter (OM) and neutral detergent fiber (NDF) were similar between treatments (P > 0.05). Although the NDF digestibility was not changed (P > 0.05), the digestibilities of DM, OM, CP, non-fibrous carbohydrates, and total digestible nutrients increased linearly (P < 0.05) with increasing CP levels in the diet. Urinary urea nitrogen excretion and nitrogen retained increased linearly (P < 0.01) with increasing CP levels. Dietary CP levels did not affect (P =0.53) energy expenditure in heifers. Ruminal ammonia-nitrogen concentration peaked at 2-4 h after feeding. Increasing dietary CP levels from 9 to 18% does not affect feed intake and energy expenditure in heifers. However, it improves the digestibility of OM and nitrogen retention.

scholarly journals Impact of Supplementation of Hemp Seeds (Cannavis sativa L.) on In Vitro Gas Fermentation Parameters and Digestibility using Foregut Fluid from Dromdary Camel

2020 ◽  
Vol 33 (1) ◽  
pp. 98-106
Author(s):  
Tahereh Mohammadabadi
Keyword(s):  
Biogas Production ◽  
Rumen Fluid ◽  
Control Diet ◽  
Nitrogen Concentration ◽  
Ammonia Nitrogen ◽  
Gas Production ◽  
Control Treatment ◽  
Cellulolytic Bacteria ◽  
Gas Fermentation

This experiment was conducted to investigate the effect of hemp seeds on in vitro biogas production, and digestibility of one-humped camel. Two female fistulated dromedary camels (300 kg) were fed with a forage diet (60 wheat straw and 40 alfalfa) for 1 month. Then rumen fluid was supplied from camels prior to the morning meal. Experimental samples were control diet and diet containing 10 % hemp seeds. Glass vials 100 ml containing 30 ml buffered rumen fluid was used. Gas production parameters were measured by exponential models and digestibility of foregut bacteria of camels were determined. The results showed that addition hemp seeds decreased potential of gas production (P<0.05), but gas production rate was not influenced (P>0.05). Actually digested organic matter and microbial biomass of diet containing hemp seeds were more than a control diet (P<0.05).While, hemp seeds supplementation had the greatest digestibility by cellulolytic bacteria of camel, after 24, 48 and 72 h incubation. The results also showed hemp seeds decreased ammonia nitrogen concentration as compared to the control treatment (P<0.05). Therefore, adding hemp seeds in the camel diet caused to reduce gas emission and ammonia nitrogen production in foregut and increased cellulolytic bacteria digestibility; so hemp seeds may be used in camel diets and will influence camel growth performance and decrease gas emissions in the environment.

scholarly journals EFFECT OF ADDITION OF DIFFERENT LEVELS OF MOLASSES AND LIQUID WHEY ON FERMENTATION CHARACTERISTICS OF DATE PALM PHOENIX DACTYLIFERA LEAVES SILAGE AND ITS NUTRITIVE VALUE

2021 ◽  
Vol 52 (4) ◽  
pp. 977-988
Author(s):  
Saeed & et al.
Keyword(s):  
Volatile Fatty Acids ◽  
Nutritive Value ◽  
Date Palm ◽  
Nitrogen Concentration ◽  
Phoenix Dactylifera ◽  
Ammonia Nitrogen ◽  
Aerobic Stability ◽  
Two Factors ◽  
Ground Wheat ◽  
Light Green

Factorial experiment was carried out to investigate the effect of two factors, molasses (M) and liquid whey (LW) on fermentation characteristics and nutritive value of date palm leaves (DPL). Fresh DPL was chopped into 1-2 cm and treated with treatment solutions prepared by addition of 5, 7.5 or 10% of M together with 3 levels of LW on basis of nitrogen content equivalent to 0, 1 or 1.5% of urea. Ground wheat straw was added to each sample at rate of 50 g. Materials were packed into double nylon sacs and ensiled for 60 days. Samples of DPL were appeared firmly connected and most of them acquired yellowish to light green color with emission of an apple-date vinegar smell. Results revealed that there was a significant (P˂0.01) decrease in dry matter and crude fiber contents with increasing M levels. In DPL silages prepared with 10% of M, these contents were 30.04 and 34.70% respectively. Crude protein content was significantly increased (P˂0.01) from 6.39 to 7.32 and 7.32% for 0, 1 and 1.5% levels of LW respectively. This was associated with a significant (P˂0.05) decrease in fat content. All samples of DPL silages were characterized with good quality as evidenced by fermentation characteristics. There was a significant (P˂0.01) decrease in pH accompanied with an increase (P˂0.01) in ammonia nitrogen concentration. Values ranged between 3.85 to 3.78 and 3.84 to 4.32% of total nitrogen for the 1st and 2nd parameters due to the addition of low and high levels of M respectively. Addition of LW at 1 and 1.5% levels decreased (P˂0.05) pH from 3.85 to 3.81 and 3.81, but concentrations of total volatile fatty acids were increased (P˂0.01) from 2.29 to 2.97 and 3.11% of DM. Results showed that increasing level of M to 7.5 and 10% increased (P˂0.01) DM loss from 12 to 17.78%, but it improved (P˂0.01) aerobic stability. Samples of DPL silages prepared with addition of 1.5% of LW resisted for longer (P˂0.01) period (55.58 hours) before signs of aerobic deterioration were appeared.

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