scholarly journals Hematological and Biochemical Reference Intervals for 5 Adult Hunting Dog Breeds Using a Blood Donor Database

Animals ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1212
Author(s):  
Arianna Miglio ◽  
Alessandra Gavazza ◽  
Donatella Siepi ◽  
Francesco Bagaglia ◽  
Ambra Misia ◽  
...  
Keyword(s):  
Blood Donor ◽  
Blood Cells ◽  
White Blood Cells ◽  
Clinical Pathology ◽  
Hemoglobin Concentration ◽  
Blood Parameters ◽  
Reference Intervals ◽  
Corpuscular Hemoglobin ◽  
Hunting Dogs ◽  
Hunting Dog

Numerous studies have shown the importance of breed-related differences between hematological and biochemical results in veterinary medicine. The aim of this study is to determine hematologic and biochemical Reference Intervals (RIs) for 5 hunting dog breeds from a blood donor database, adopting an indirect sampling method, and to compare them with laboratory established and published RIs to identify possible breed and attitude-related differences. The study analyzed the blood parameters of 445 adults (222 females and 223 male, with age ranging from 2 to 8 years, mean age 5.3 years), client-owned, clinically healthy blood donor dogs of 5 breeds: 156 Ariégeois, 52 Bleu de Gascogne, 64 Bracco italiano, 123 Segugio italiano, and 50 Briquet Griffon Vandeen. Statistical analysis was performed as recommended by the American Society of Veterinary Clinical Pathology (ASVCP) guidelines. RIs for red blood cells (RBC), hematocrit (HCT), hemoglobin (HB), main corpuscular volume (MCV), main corpuscular hemoglobin (MCH), main corpuscular hemoglobin concentration (MCHC), red distribution widht (RDW), white blood cells (WBC), and differential leukocytes count, PLT, Albumin, Total Protein, Urea, Creatinine, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) for each of the 5 breeds were performed, and significant differences with the established RIs were detected. We found significant differences in 12 hematologic and serum biochemical analytes for which a breed-specific variation appears to be the most plausible explanation. New RIs for HCT, MCH, MCHC, RDW, PLT, Monocytes, Eosinophils, Albumin, Urea, Creatinine, AST, and ALT are provided for at least 1 breed. Breed-specific RIs for adult hunting dogs will help avoid misinterpretation of laboratory results in these breeds.

scholarly journals Changes in hematological parameters and gill morphology in common carp exposed to sub-lethal concentrations of Malathion

2016 ◽  
Vol 2 (3) ◽  
pp. 370-378 ◽  
Author(s):  
Sadia Sharmin ◽  
Md Abdus Salam ◽  
Farhana Haque ◽  
Md Sadiqul Islam ◽  
Md Shahjahan
Keyword(s):  
Common Carp ◽  
Blood Cells ◽  
Morphological Changes ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Blood Parameters ◽  
Mean Corpuscular Hemoglobin ◽  
Agricultural Field ◽  
Corpuscular Hemoglobin

Malathion is an organophosphorous insecticide widely used in the agricultural field in Bangladesh and reach in the aquatic environment through rain wash. In the present study, we examined the effects of Malathion on hematological parameters and gills morphology in common carp exposed to two sub-lethal concentrations (1.5 and 3.0 mg/L) for a period of 192h (8 days). For hematological parameters fish were sacrificed at 24, 48, 72, and 96h after start of exposure. Gills were collected at 192h after start of exposure. The blood glucose level was significantly elevated with increasing the concentration of Malathion. Red blood cells (RBCs), hemoglobin (Hb) and hematocrit (Hct) values were significantly decreased after the exposure of Malathion. Significant increments were observed in case of the mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), while no distinct changes were noted for mean corpuscular hemoglobin (MCH). Interestingly, white blood cells (WBCs) count was significantly increased with toxicity of Malathion. Several morphological changes, such as telangiectasia, blood lamellar congestion, hypertrophy of filaments, lamellar fusion were observed in the gills of fish exposed to Malathion. The present study revealed that the insecticide had adverse effects on various blood parameters and gills morphology in common carp. Thus, the use of insecticide in the agriculture field may be a threat to fauna and flora of the aquatic environment.Asian J. Med. Biol. Res. September 2016, 2(3): 370-378

scholarly journals CLINICOPATHOLOGICAL EFFECTS OF HEAT STRESS ON BLOOD PICTURE OF JAPANESE QUAILS

2021 ◽  
Vol 9 (5) ◽  
pp. 913-916
Author(s):  
Khurshaid Anwar ◽  
◽  
Nakash Smark ◽  
Sartaj Khan ◽  
Kalim Ullah ◽  
...  
Keyword(s):  
Heat Stress ◽  
Blood Cells ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Coturnix Japonica ◽  
Mean Corpuscular Hemoglobin ◽  
Coturnix Coturnix Japonica ◽  
Corpuscular Hemoglobin ◽  
Coturnix Coturnix ◽  
Japanese Quails

This study aimed to evaluate the effects of heat stress on quail hematological parameters two published papers on quails in 2013 were studied, analyzed and compared. Broiler Japanese quails (Coturnix coturnix japonica) were exposed to high ambient temperature (340C, 8hr/d, 0900-1700hr) in both papers. An increase in values of red blood cells count, white blood cells count, lymphocytes, heterophils and lymphocytes (H/L) ratio, Monocytes, packed cell volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration were recorded while a lower values of heterophils, basophils, eosinophils, mean corpuscular volume and hemoglobin concentration than normal were recorded in groups exposed to heat stress (340C) on day 42 in Japanese quails (Coturnix coturnix japonica). Hemoglobin concentration was least affected. The obtained results indicated that, rearing quail birds under high environmental conditions during the fattening period has a great adverse effect on blood parameters. To help maintain Japanese quail as an essential animal model further heat stress related research on modern ways is needed.

scholarly journals Hematological parameters of Iranian cichlid Iranocichla hormuzensis: Coad, 1982 (Perciformes) in Mehran River

2012 ◽  
Vol 84 (4) ◽  
pp. 943-949 ◽  
Author(s):  
Ehsan Daneshvar ◽  
Mahsa Y. Ardestani ◽  
Salar Dorafshan ◽  
Mauricio L. Martins
Keyword(s):  
Blood Cells ◽  
Mean Corpuscular Volume ◽  
High Efficiency ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Food Fish

This study describes the hematological parameters in Iranocichla hormuzensis, an Iranian freshwater cichlid important as ornamental and food fish. Forty fish were captured with seine net at Mehran river Hormozgan province, Iran. Blood was used to determine the total counts of red blood cells (RBC) and white blood cells (WBC), hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and morphometric data of erythrocytes. The Iranian fish showed lower RBC and WBC values than the other cichlids (Oreochromis niloticus, O. aureus, O. mossambicus, O. hybrid, Cichlasoma dimerus and Cichla monoculus). Hematocrit did not vary among the species, but MCV, MCH and MCHC in I. hormuzensis were higher than those for O. niloticus, O. aureus, O. hybrid, C. dimerus and C. monoculus. These differences may be related to different life habit of fish. This study suggests that I. hormuzensis is well acclimated to the environment being the first report for its hematology. It is also suggested high efficiency in oxygen transportation, and an efficient inflow of oxygen by the gills, indicating the welfare of fish on this environment.

scholarly journals Effect of ketorolac administration post bone cavitation surgery on some hematological parameters

2017 ◽  
Vol 41 (1) ◽  
pp. 169-177
Author(s):  
Raffal A. Omar
Keyword(s):  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Treated Group ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Post Operation

     This study was designed to determine the effect of ketorolac on some hematological parameters post cavitation surgery in domestic rabbits. Ninety-six adult domestic local breed male rabbits were used in this study, weighing (1.23 ± 0.33) kg. They were divided randomly into two groups in the first one (n= 48/group) which represented control group, while the second one (n=48/group) represented the treated group (Ketorolac group). All rabbits in both groups undergoes surgical operation (bone cavitation in femur bone) under general anesthesia. In the Ketorolac group, animals were treated by using 30 mg/kg of Ketorolac directly post-operation and continued daily for 5 days, while in Control group no treatment was given postoperatively. The hematological parameters were recorded which include white blood cells count; red blood cells count; hemoglobin; packed cell volume; mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration; mean corpuscular volume and platelets at the period of (3, 5, 7, 14, 21, 28, 35, and 42) days post operation were estimated. Results concerning hematological values showed no significant differences in packed cell volume values between control and treated group and within each group, while haemoglobin values showed a significant decrease at period 7 and 14 days between control and treated group. Within control group the significant decrease were noticed clearly at period of 7 days. Data concerning mean corpuscular hemoglobin values within control group showed significant decrease were noticed at 5 day, but within treated group, there were no significant differences. mean corpuscular hemoglobin concentration values showed significant decrease ate period 3; 5 and 21 days between control group and treated group respectively. Within control group the significant decrease were noticed obviously at 5 days while within treated group the significant decrease were noticed at 35 days. mean corpuscular volume values showed significant increase at 3 and 5 days period between control and treated groups. Platelets counts within control group showed significant increase at period of 14 days, within treated group the significant increase were recorded at 42 days. Red blood cells showed significant decrease between control and treated groups, while within control group, the significant decrease were at 7 days. White blood cells count within treated group showed significant increase recorded at 21 days. Differential White blood cells count concerning Lymphocytes cells within control group the significant increase were recorded at 42 days  while the significant decrease were noticed at 21 days, but within treated group the significant increase were recorded at 35 days  and significant decrease were noticed at 14 days. Neutrophils counts showed significant decrease at periods 3, 14, 28 and 42 days between control and treated groups respectively; while within control group there were significant decrease at 3 days, but within treated group the significant decrease were noticed at 42 days. Monocytes cells showed significant increase at 35 days between control and treated groups, within control group there were significant increase at 35 days, while within treated group the significant increase recorded at 7 days.

Effect of gender on hematological parameters in growing Ouled Djellal sheep of semi arid zones of Northeastern Algeria

2021 ◽  
Vol 11 (4) ◽  
pp. 407-417
Author(s):  
Zerari Aicha ◽  
Mahdi Djahida ◽  
Allaoua Sofia Amel ◽  
Rouar Salim
Keyword(s):  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Arid Zones ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Mean Values ◽  
Ewe Lambs

Ouled Djellal sheep is the most dominant breed in Algeria due to its adapta-tion to harsh steppe environment. Hematological parameters are good indi-cators of animal health, but their values are breed-specific, and they are affected by age and gender. Therefore, this study aimed to determine the hematological profile of healthy Ouled Djellal lambs and ewe lambs reared traditionally under semi-arid zones of Algeria during the growing peri-od. Blood samples were taken from twenty-four Ouled Djellal lambs (12 males, 12 females) at 42, 63, 84 and 105 days of age to evaluate the count of White Blood Cells, Lymphocyte, Monocyte, Granulocyte, Red Blood Cell and total Platelet, the percentage of Lymphocyte, Monocyte and Granulocyte, Hemoglobin concentration, Hematocrit, Mean Corpuscular Volume, Mean Corpuscular Hemoglobin and Mean Corpuscular Hemoglobin Concentration by usual known methods. Both male and female lambs did not show signifi-cant variations in mean values of White Blood Cells, Lymphocyte count, Red Blood Cells, Hemoglobin, Mean Corpuscular Hemoglobin concentration, per-centage of Monocyte, Monocyte count, Gran percentage and Granulocyte count from 42ͭ ͪ day to 105ͭ ͪ days. Mean values of percentage of Lymphocyte, Hematocrite in lambs, Mean Corpuscular Hemoglobin in ewe lambs, and Mean Corpuscular Volume, Platelets levels in both lambs and ewe lambs indicated also variations during this period of growth. Significant Pearson's correlations were found between some hematological parameters in Ouled Djellal lambs and/or ewe lambs. Therefore, these findings provide usual val-ues, which can be considered for assessing the physiological, nutritional and health status of these animals.

scholarly journals The toxic effects of ammonia on growth rate and some blood parameters in common carp (Cyprinus carpio L.)

2018 ◽  
Vol 11 (1) ◽  
pp. 109-113
Author(s):  
Q Jumaa ◽  
K Al-Saeedy ◽  
W Obid
Keyword(s):  
Treatment Group ◽  
Common Carp ◽  
Blood Cells ◽  
Negative Impact ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Blood Parameters ◽  
Control Group ◽  
Common Carp Cyprinus Carpio ◽  
Fish Breeding

This study was performed to know the effect of ammonia on the blood picture and the weight of common carp fish. 50 fish were used at the age of 6 months and were divided into two groups. Each group included 25 fish. The first group represented the treatment group with ammonia at a concentration of 1.0 mg/ L. and the second group represented control group. It is grown in plastic basins with dimensions of 1.5 × 2 meters and continued treatment for four weeks. It was observed that the weight in the ammonium treatment group had the lowest significant value (p≤0.05) compared to the control group. It was also observed in this study that the ammonia group had the lowest significant values in (white blood cells, red blood cells, hemoglobin concentration and Packed cell volume) compared with control group. As for the biochemical parameters, it was observed that the Glucose concentration and each of the enzymes Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST) had a record the highest value in the group treated with ammonia compared with the control group while the total protein recorded the lowest significant value in the group treated with ammonia Compared to control group. We conclude from this study that increasing the concentration of ammonia in the aquatic environment has a negative impact on fish breeding and health.

scholarly journals HEMATOLOGICAL AND SERUM BIOCHEMICAL PROFILE IN EAST BALKAN PIGS AT DIFFERENT AGE AND SEASONS

AGROFOR ◽  
2019 ◽  
Vol 4 (3) ◽  
Author(s):  
Nadezhda PALOVA ◽  
Yordan MARCHEV ◽  
Radka NEDEVA ◽  
Jivko NAKEV ◽  
Dimitrinka KRUSHEVA ◽  
...  
Keyword(s):  
Blood Cells ◽  
Biochemical Parameters ◽  
White Blood Cells ◽  
Age Groups ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Biochemical Profile ◽  
Corpuscular Hemoglobin ◽  
Serum Biochemical ◽  
Serum Biochemical Profile

Twelvehematological and fifteen serum biochemical parameters were determined in indigenous East Balkan pigs at different ages and two seasons. The experiment was carried out in the Scientific Centre of Agriculture- Sredets aiming to characterize the health status of the animals when reared organically. Blood samples were taken from pre-weaned piglets (n=10), growers (n=10) and sows (n=10) in spring and summer. The results were analysed through two way ANOVA to assess the influence of the age, season and their interaction on the hematological and serum biochemical profile of the animals. Both age and season interacted significantly in regard to the red blood cells count (RBC) (P0.001), hemoglobin (HGB) (P0.001) and hematocrit (HCT) (P0.01), as well as in the most biochemical parameters including creatinine(P0.001), total protein (TP) (P0.001), albumin (ALB) (P0.001), alanine aminotransferase (ALT) (P0.001), aspartate aminotransferase (AST) (P0.01), uric acid (UA) (P0.01), Mg (P0.001), triglycerides (TG) (P0.05) and cholesterol (P0.01). Regardless of the season, the white blood cells (WBC) including lymphocytes and granulocytes, as well as platelets (PLT)decreased with age, while mean corpuscular volume (MCV) increased.The content of glucose, urea, gamma-glutamil transferase (GGT), alkaline phosphatase (ALP), Ca and P which displayed maximal values in preweaned pigs (P0.05) compared to the other age groups (P0.05). The season affected the number of lymphocytes (P0.01), their percent (P0.001), mean corpuscular hemoglobin (MCH) andmean corpuscular hemoglobin concentration (MCHC) (P0.001), as well as the concentration of GGT (P0.001) which increased during summer,and also granulocytes (P0.01), their percentage (P0.001), PLT (P0.05), glucose, urea, ALP, Ca (P0.001) and P (P0.01) whichwere higher in spring.

Copper and Copper Nanoparticles Induced Hematological Changes in a Freshwater Fish Labeo rohita – A Comparative Study

2016 ◽  
pp. 352-375
Author(s):  
Kaliappan Krishnapriya ◽  
Mathan Ramesh
Keyword(s):  
Blood Cells ◽  
Mean Corpuscular Volume ◽  
Labeo Rohita ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Treated Fish ◽  
Hematological Changes

In the present study, fish Labeo rohita were exposed to 20, 50 and 100 µg/L of both Cu NPs and copper sulphate (CuSO4, bulk copper) for 24 h and hematological profiles were estimated. A significant (P< 0.01) change in the hemoglobin (Hb), hematocrit (Hct), white blood cells (WBC) and Mean Corpuscular Volume (MCV) levels were observed in all the three concentrations of both bulk and Cu NPs treated fish when compared to control groups. However a non significant change in red blood cells (RBC) (20 and 50 µg/L Cu NPs) and mean corpuscular hemoglobin (MCH) (20 and 50 µg/L bulk Cu) were observed. The alteration in Mean Corpuscular Hemoglobin Concentration (MCHC) value was found to be non significant both in bulk and Cu NPs treated fish. The alterations of these parameters can be used as a potential indicator to examine the health of fish in aquatic ecosystem contaminated with metal and metal based nanoparticles.

scholarly journals AMELIORATIVE ROLE OF BEE HONEY AND ROYAL JELLY AGAINST CISPLATIN INDUCED ALTERATION IN HEMATOLOGICAL PARAMETERS IN MALE WISTER ALBINO RAT

2018 ◽  
Vol 10 (4) ◽  
pp. 110 ◽  
Author(s):  
Waykar Bhalchandra ◽  
Yahya Ali Alqadhi ◽  
A. S. Ninawe
Keyword(s):  
Blood Cells ◽  
Royal Jelly ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Control Group ◽  
Corpuscular Volume ◽  
Corpuscular Hemoglobin ◽  
Mean Values

Objective: This study aims to investigate the ameliorative role of dietary bee honey and royal jelly against cisplatin-induced alterations in hematological parameters in male wistar albino rat.Methods: Male wistar albino rats of same age and weight were randomly divided into four groups; G, I: control group which was given 0.9% saline, G: II: cisplatin (7 mg/kg/d) was injected intraperitoneally for 15 d, G, III bee honey with royal jelly (500 mg/kg/d of honey and 100 mg/kg/d of royal jelly) fed orally daily for 15 d, G, IV: cisplatin (7 mg/kg/d) was injected intraperitoneally and honey (500 mg/kg/d) and royal Jelly (100 mg/kg/d) fed orally daily for 15 d. The hematological parameters like total number of white blood cells (WBCs), red blood cells (RBCs), platelets, % of hemoglobin (Hb), and mean values of packed cell volume (PCV), mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC) were measured by using automated hematology system.Results: Cisplatin treated rats revealed a significant decrease in total number of white blood cells (WBCs), red blood cells (R. B. Cs), platelets, percentage of hemoglobin (Hb), and mean values of packed cell volume (PCV), corpuscular volume (MCV) and corpuscular hemoglobin concentration (MCHC) as compared to control group. Royal jelly and honey treated group of rats revealed a significant increase in all blood parameters compared to control group. Dietary bee honey with royal jelly along with cisplatin-treated rats revealed significant increas as compared to animals treated with cisplatin (G, II) and the computed significant valus for the above parameters are 10.00, 2.30, 8,54, 12.00, 35.00, 47.40 and 32,30 respectively.Conclusion: Bee honey and royal jelly could be used as dietary preventive natural products against cisplatin-induced hematological alterations during the treatment of cancer.

Hematological values in bradypus variegatus (Schinz, 1825) from creatories of the Belém metropolitan region

2019 ◽  
Vol 10 (5) ◽  
pp. 82-88 ◽  
Author(s):  
Gilmar Rodrigues Galvão ◽  
Elane Guerreiro Giese ◽  
Gilvando Rodrigues Galvão ◽  
Luiz Fernando Moraes Moreira ◽  
Maridelzira Betânia Moraes David ◽  
...  
Keyword(s):  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Cell Types ◽  
Metropolitan Region ◽  
Corpuscular Hemoglobin ◽  
Differential Leukocyte Count ◽  
Bradypus Variegatus ◽  
Hematological Values

The blood tissue is made up of three main cell types: erythrocytes (red blood cells), leukocytes (white blood cells) and platelets (thrombocytes), suspended in a liquid phase named plasma. In addition to the cells, blood is composed of proteins, inorganic and organic substances, hormones and various components. The captive condition can also predispose the animal to a number of conditions, making it necessary to carry out laboratory tests for the biomonitoring of the animals. Among these tests, the complete hemogram stands out. This is done to assess the general health, guiding and deepening the nature of pathophysiological situations besides its utility as an aid to diagnosis and prognosis of certain diseases. The objective of this study is to describe the hematological values of brown-throated sloth (Bradypus variegatus). Hence, eleven adult animals of both sexes (seven males and four females) at appropriate health conditions were used in the experiment. Blood was collected from the cephalic vein and analyzed through veterinary hematology counter. The evaluate hematological parameters included total count of erythrocytes and leukocytes, hematocrit, hemoglobin concentration, average corpuscular volume, average corpuscular hemoglobin, average corpuscular hemoglobin concentration differential leukocyte count and platelet count. In general, the values did not differ from few studies in the literature. However, the score of platelets was greater in males than in females. The results achieved in the study can serve as a basis in determination and interpretation as hematologic references to brown-throated sloths in Para Amazon.

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