scholarly journals Effect of Lipopolysaccharide and Muramyl Dipeptide on Apoptosis of Bovine Mammary Gland Lymphocytes

Animals ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 990
Author(s):  
Petr Slama ◽  
Eliska Kabourkova ◽  
Zbysek Sladek ◽  
Terezie Zavadilova ◽  
Lucie Kratochvilova ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Mammary Gland ◽  
Inflammatory Response ◽  
Initial Phase ◽  
Muramyl Dipeptide ◽  
Mammary Glands ◽  
Cell Populations ◽  
Bovine Mammary Gland ◽  
Lymphocyte Apoptosis ◽  
Cd44 Expression

The aim of this study was to evaluate whether apoptosis of lymphocytes is modulated by stimulation by lipopolysaccharide (LPS) of Escherichia coli or muramyl dipeptide (MDP). Cell populations were obtained by lavaging of the mammary glands 24, 48, 72, and 168 h following intramammary induced inflammation. The portion of apoptotic lymphocytes peaked at 48 h after treatment with LPS or MDP. The analysis of CD44 expression of the same cell populations showed a higher percentage of CD44-positive lymphocytes 24- and 48-h following induction of inflammation by LPS or MDP. The results demonstrate that during both experimental infection of bovine mammary glands with LPS or MDP, apoptosis of lymphocytes was induced in the initial phase of the inflammatory response and CD44 was also overexpressed at the beginning of inflammation. These data suggest a connection of lymphocyte apoptosis with the expression of CD44 receptors.

scholarly journals The dynamics of morphological changes during in vitro aging of bovine virgin mammary gland neutrophils

2012 ◽  
Vol 47 (No. 12) ◽  
pp. 325-332 ◽  
Author(s):  
Z. Sládek ◽  
D. Vašíčková ◽  
D. Ryšánek
Keyword(s):  
Mammary Gland ◽  
Trypan Blue ◽  
Morphological Changes ◽  
Membrane Integrity ◽  
Mammary Glands ◽  
Progressive Increase ◽  
Apoptotic Bodies ◽  
Bovine Mammary Gland ◽  
Cell Counts

The present study was an in vitro analysis of the dynamics of bovine mammary gland neutrophil apop­tosis based on the detection of morphological changes. The neutrophils were isolated from mammary glands of five virgin heifers. The mammary glands were lavaged, the suspensions were then bacteriologically examined, and total and differential cell counts were made. The cells were cultivated in vitro for 4 hours. After 2, 3 and 4 hours of cultivation, they were panoptically stained, and the proportions of apoptotic neutrophils and trypan blue positive neutrophils were determined. Neutrophil apoptosis and impaired cytoplasmic membrane integrity of neutrophils were already observed in the mammary gland lavages (11.9% and 0.8%, respectively). During the cultivation, a progressive increase in the number of apoptotic neutrophils in various stages of apoptosis – karyopyknosis, zeiosis and apoptotic bodies – was observed. Karyopyknotic neutrophils represented a dominant part of the apoptotic neutrophil population in the course of the whole cultivation. The most intensive increase was observed in zeiosis, whereas the levels of apoptotic bodies remained the same. After 4 hours of cultivation, 31.7% apoptotic neutrophils and 9.8% trypan blue positive neutrophils (i.e. Secondary necrotic cells) were found. The results of this work show that spontaneous apoptosis and secondary neutrophil necrosis must be taken into account during in vitro cultivations of bovine mammary gland neutrophils.

scholarly journals MtuA, a Lipoprotein Receptor Antigen from Streptococcus uberis, Is Responsible for Acquisition of Manganese during Growth in Milk and Is Essential for Infection of the Lactating Bovine Mammary Gland

2003 ◽  
Vol 71 (9) ◽  
pp. 4842-4849 ◽  
Author(s):  
Amanda J. Smith ◽  
Philip N. Ward ◽  
Terence R. Field ◽  
Catherine L. Jones ◽  
Ruth A. Lincoln ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Inflammatory Response ◽  
Metal Binding ◽  
Parental Strain ◽  
Insertional Mutagenesis ◽  
Bovine Milk ◽  
Bovine Mammary Gland ◽  
Streptococcus Uberis ◽  
Efficient Uptake ◽  
Single Insertion

ABSTRACT A mutant strain of Streptococcus uberis (AJS001) that was unable to grow in bovine milk was isolated following random insertional mutagenesis. The level of growth in milk was restored to that of the parental strain (strain 0140J) following addition of MnSO4 but not following addition of other metal ions. The mutant contained a single insertion within mtuA, a homologue of mtsA and psaA, which encode metal-binding proteins in Streptococcus pyogenes and Streptococcus pneumoniae, respectively. Strain AJS001 was unable to infect any of eight quarters on four dairy cows following intramammary challenge with 105 CFU. Bacteria were never recovered directly from milk of these animals but were detected following enrichment in Todd-Hewitt broth in three of eight milk samples obtained within 24 h of challenge. The animals showed no inflammatory response and no signs of mastitis. Three mammary quarters on two different animals simultaneously challenged with 600 CFU of the parental strain, strain 0140J, became colonized, shed high numbers of S. uberis organisms in milk, displayed a marked inflammatory response to infection, and showed overt signs of mastitis. These data indicate that mtuA was required for efficient uptake of Mn2+ during growth in bovine milk and infection of the lactating bovine mammary gland.

Role of several Staphylococcus aureus virulence factors on the inflammatory response in bovine mammary gland

2006 ◽  
Vol 40 (4) ◽  
pp. 177-183 ◽  
Author(s):  
Alfonso Zecconi ◽  
Lorenza Cesaris ◽  
Emmanouil Liandris ◽  
Valentina Daprà ◽  
Renata Piccinini
Keyword(s):  
Staphylococcus Aureus ◽  
Mammary Gland ◽  
Inflammatory Response ◽  
Virulence Factors ◽  
Bovine Mammary Gland

Bacterial Adherence in Mastitis Caused by Escherichia coli

1977 ◽  
Vol 14 (6) ◽  
pp. 618-628 ◽  
Author(s):  
J. C. Anderson ◽  
M. R. Burrows ◽  
A. J. Bramley
Keyword(s):  
Escherichia Coli ◽  
Mammary Gland ◽  
Resistant Strain ◽  
Clinical Mastitis ◽  
Bacterial Adherence ◽  
Bovine Mammary Gland ◽  
E Coli ◽  
Mild Disease ◽  
Highly Virulent

The possible role of bacterial adherence in the pathogenesis of experimental mastitis in the mouse was examined with four strains of Escherichia coli. Two of these strains had a known adhesion antigen (K88) and two did not. The K88 antigen did not play a significant role in the virulence or infectivity of E. coli either in the murine or bovine mammary gland. Two E. coli strains, W1 (K88+) and J2 (K88−) were virulent in the mouse but did not adhere to epithelial cells. Both these strains produced clinical mastitis in the cow. A third strain, D282 (K88−), produced mild disease in the mouse but was avirulent in the cow. The fourth strain, 233/1D (K88+), was avirulent in both the mouse and the cow. Strains D282 and 233/1D were killed rapidly by bovine serum whilst J2 and W1 were more resistant. All strains were more sensitive than the control resistant strain E. coli P4, which is known to be highly virulent for the lactating udder.

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