scholarly journals The Implications of EU Regulation 2016/429 on Neglected Diseases of Small Ruminants including Contagious Agalactia with Particular Reference to Italy

Animals ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 900
Author(s):  
Guido Ruggero Loria ◽  
Luigi Ruocco ◽  
Gabriele Ciaccio ◽  
Francesco Iovino ◽  
Robin A. J. Nicholas ◽  
...  
Keyword(s):  
Animal Health ◽  
Small Ruminants ◽  
Foot And Mouth Disease ◽  
African Swine Fever ◽  
Mouth Disease ◽  
Neglected Diseases ◽  
The European Union ◽  
Animal Diseases ◽  
Foot And Mouth ◽  
The Eu

After almost 40 years, the 27 member states (MS) of the European Union (EU) will comply with the European Law 429/2016 in 2021 by completing a process of unification and harmonization of all regulations related to animal health between MS. These new provisions are based on modern scientific principles on animal health, on long-term epidemiological data, and, above all, on the most current risk assessment and analysis. The paper describes all changes and updates, which will impact the Italian current National regulation. A total of 58 animal diseases have been included in the Annex II (“Listing”) and Annex IV (“Categorization”) of the new Delegated Act (DA 2018/1629). Five diseases comprising the great viral epizooties were automatically included on the list because of their primary importance. These diseases include foot and mouth disease (FMD), African swine fever (ASF), classical swine fever (CSF), highly pathogenic avian influenza (HPAI), and African horse sickness (AHS). Another 53 diseases have been identified by the ad hoc assessment on listing and categorization of animal diseases developed by the European Food Safety Association. Seventeen communicable diseases of the Order Artiodactlya (sheep, goats, deer, etc.) have been listed including foot and mouth disease, sheep and goat pox, and pestes de petits ruminants. In addition, other endemic diseases affecting more than one species include blue tongue, tuberculosis, brucellosis, and anthrax. There are five categories (A-E) based on the degree of action to be undertaken throughout the EU for each disease. These vary from complete eradication for diseases not normally found in the EU like FMD (category A) for establishing surveillance for diseases like West Nile that present high risk but lack control tools (category E).

Assessment of socio-economic impacts of Foot-and-Mouth Disease on Cattle in Twelve Villages of Cambodia

2014 ◽  
Author(s):  
Keyword(s):  
Economic Impact ◽  
Economic Impacts ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Impact Study ◽  
Animal Diseases ◽  
Foot And Mouth ◽  
Socio Economic Impact

This socio-economic impact study on foot and mouth disease (FMD) in Cambodia is part of the regional initiative funded by AusAID called Stop Transboundary Animal Diseases and Zoonoses (STANDZ), which is coordinated by OIE SRR-SEA.

scholarly journals Investigations into Foot and Mouth Disease Outbreak among Small Ruminants in Karnataka State of India

2021 ◽  
Author(s):  
Raveendra Hegde ◽  
B.P. Shivashankar ◽  
N. Gautham ◽  
G.R. Praveenkumar ◽  
B. Rajasekar ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Small Ruminants ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Perivascular Spaces ◽  
Grazing Land ◽  
Sheep Population ◽  
Goat Population ◽  
Karnataka State ◽  
Foot And Mouth

Background: Foot-and-Mouth Disease (FMD) remains a serious threat to the Indian livestock sector due to significant economic loss associated with it. Systematic vaccination of large ruminants over the years has lead to a gradual reduction in the number of disease outbreaks in India. However exposure to FMDV infection in small ruminants has been recorded during the past few years (Rout et al. 2013). Sheep and goat population have not been vaccinated so far against FMD under the FMD-Control program (FMD-CP). The present study highlighted the outbreak of FMD in small ruminants in Karnataka, India.Methods: During the period 2018-19, seven suspected FMD outbreaks among sheep population in Bellary and Tumakuru districts of Karnataka state were investigated. Tongue epithelium (oral swabs) and foot lesions (n=23) from clinically affected sheep and tissues such as heart, lung, liver, spleen, lymph nodes and kidneys from lambs during post mortem (n=67) were collected. All the samples were processed in the laboratory for the detection of FMD virus antigen by employing Serotype differentiating antigen detection ELISA and by multiplex PCR. Heart tissue samples were also collected in buffered formalin for histopathology study and processed by routine paraffin embedding technique and stained with Hematoxylin and Eosin (H and E). Serum samples from the recovered animals were collected and screened by NSP-ELISA and LPB-ELISA to check the antibody status in the affected herd. Result: A total of seven suspected outbreaks of FMD involving 688 small ruminants was investigated. The outbreak of FMD due to FMDV serotype O was confirmed by ELISA and multiplex PCR assays. Clinically, the affected adult sheep showed typical signs of FMD, while mortality in young lambs was observed without apparent signs of disease. Histologically, heart tissues from FMD affected lambs showed myocardial necrosis with marked aggregations of lymphocytes and neutrophils in the myocardium and perivascular spaces. History of FMD outbreaks in cattle and common grazing land for the livestock, as well as sheep within the reach of these villages, may be the major contributing factors for the outbreaks in sheep populations.

Socio-Economic Impacts of Foot and Mouth Disease Among Cattle Farmers in Sagaing and Mandalay Areas, Myanmar

2014 ◽  
Author(s):  
Ai Thanda Kyaw
Keyword(s):  
Smallholder Farmers ◽  
Economic Impacts ◽  
Animal Health ◽  
Lao Pdr ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Economic Costs ◽  
Foot And Mouth ◽  
World Organisation ◽  
Control And Eradication

The World Organisation for Animal Health (OIE) Sub-Regional Representation for South East Asia (OIE SRR-SEA) implemented the Stop Transboundary Animal Diseases and Zoonoses (STANDZ) Programme funded by AusAID to strengthen the veterinary services and effectively manage the control and eradication of foot and mouth disease (FMD) in Cambodia, Lao PDR and Myanmar. The purpose of the study is to understand how FMD outbreaks impact smallholder farmers, both men and women, at the household and village level and how control and eradication of FMD would benefit them. Specific aims are to estimate the direct and indirect socio-economic costs associated with the outbreaks of FMD as well as of the measures taken by farmers to deal with such outbreaks and to identify issues that contributed to the socio-economic impacts of FMD outbreaks and opportunities to reduce them.

scholarly journals Assessing the value of PCR assays in oral fluid samples for detecting African swine fever, classical swine fever, and foot-and-mouth disease in U.S. swine

PLoS ONE ◽  
2019 ◽  
Vol 14 (7) ◽  
pp. e0219532 ◽  
Author(s):  
Oriana Beemer ◽  
Marta Remmenga ◽  
Lori Gustafson ◽  
Kamina Johnson ◽  
David Hsi ◽  
...  
Keyword(s):  
Oral Fluid ◽  
Foot And Mouth Disease ◽  
African Swine Fever ◽  
Classical Swine Fever ◽  
Mouth Disease ◽  
Foot And Mouth ◽  
Pcr Assays

scholarly journals Epidemiological study on foot-and-mouth disease in small ruminants: sero-prevalence and risk factor assessment in Kenya

2020 ◽  
Author(s):  
Eunice C. Chepkwony ◽  
George C. Gitao ◽  
Gerald M. Muchemi ◽  
Abraham K. Sangula ◽  
Salome W. Kairu-Wanyoike
Keyword(s):  
Risk Factors ◽  
Virus Transmission ◽  
Small Ruminants ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Cluster Sampling ◽  
Serum Samples ◽  
Cross Sectional ◽  
Production Type ◽  
Foot And Mouth

AbstractFoot-and-mouth disease (FMD) is endemic in Kenya affecting cloven-hoofed ruminants. The epidemiology of the disease in small ruminants (SR) is not documented. We carried out a cross-sectional study, the first in Kenya, to estimate the sero-prevalence of FMD in SR and the associated risk factors nationally. Selection of animals to be sampled used a multistage cluster sampling approach. Serum samples totaling 7564 were screened for FMD antibodies of Non-Structural-Proteins using ID Screen® NSP Competition ELISA kit. Data were analysed using Statistical Package for Social Studies Version 20. To identify the risk factors, chi-square and logistic regression analyses were used. The country animal level sero-prevalence was 23.3% (95% CI: 22.3-24.3%) while herd level sero-prevalence was 77.6% (95% CI: 73.9-80.9%). Sero-positivity was significantly higher in the pastoral zone (31.5%) than in the sedentary zone at 14.5% (χ2 =303.2, p<0.05). In the most parsimonious backward fitting logistic multivariable regression, the only risk factors that were significantly positively associated with FMD sero-positivity in SR were multipurpose (OR=1.150; p=0.034) and dairy production types (OR=2.029; p=0.003). Those that were significantly negatively associated with FMD sero-positivity were male sex (OR=0.856; p=0.026), young age (OR=0.601; p=0.037), sedentary production zone (OR=0.471; p<0.001), bringing in of SR (OR=0.838; p=0.004), purchase of SR from market/middlemen (OR=0.877; p=0.049), no interaction with wildlife (OR=0.657; p<0.001), mixed production type (OR=0.701; p=0.016), enclosure of SR day and night (OR=0.515; p=0.001), migratory grazing system (OR=0.807; p=0.047), on-farm watering system (OR=0.724; p=0.002), male-from-another-farm (OR=0.723; p=0.030) and artificial insemination (OR=0.357; p=0.008) breeding methods.This study showed that there is widespread undetected virus circulation in SR indicated by ubiquitous spatial distribution of significant FMD sero-positivity in the country. The risk factors were mainly husbandry related. Strengthening of risk-based FMD surveillance in carrier SR which pose potential risk of virus transmission to other susceptible species is recommended. Adjustment of husbandry practices to control FMD in SR and in-contact species is suggested. Cross-transmission and more risk factors need to be researched.

scholarly journals An Assessment of Microbiological Methods to Test Sterility of Foot-and-mouth Disease Vaccines Produced in Brazil

2018 ◽  
Vol 44 (1) ◽  
pp. 5
Author(s):  
Carla Rosane Rodenbusch ◽  
Luiz Roberto da Silveira ◽  
Álvaro Ricardo Bavaresco ◽  
Marcus Vinícius Burgel Sfoggia
Keyword(s):  
Membrane Filtration ◽  
Culture Medium ◽  
Animal Health ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Pump System ◽  
Microbiological Methods ◽  
Foot And Mouth ◽  
Direct Inoculation ◽  
Filtration Technique

Background: Foot-and-mouth vaccines are an important tool in the control and eradication of the disease. In order to be commercialized, vaccines produced in Brazil undergo an evaluation process by health authorities, which includes sterility testing, residual active virus, potency, thermal stability, volume and non-structural protein activity. Sterility tests described in the Brazilian Pharmacopeia and by the World Organization for Animal Health (OIE) include direct inoculation and membrane filtration methods. The objective of the present study was to evaluate these two methods used to analyze sterility of vaccines against foot-and-mouth disease produced in Brazil.Materials, Methods & Results: Vaccines produced by the six main laboratories in Brazil were initially tested for filtration capacity. The sensitivity of the two techniques was determined artificially contaminating vaccines using known bacterial concentrations. Vaccines (9 bottles) from the same manufacturer were inoculated with 5 mL of steady-state growths of Pseudomonas aeruginosa, Candida albicans, and Clostridium sporogenes to final concentrations of 0.1, 1 and 10 CFU/ mL and a final volume of 55 mL. Bottles were manually shaken for 1 min to complete homogenization of contents. Then, 10 mL of each flask were used in assessment of the direct inoculation method, and 10 mL were used to evaluate the membrane filtration technique. Direct inoculation was carried out inoculating 1 mL of the experimentally contaminated vaccine in five tryptic soy broth (TSB) and fluid thioglycollate medium (FTM) bottles. The membrane filtration technique was carried out filtrating 10 mL of the challenged vaccines in a peristaltic pump system (SteritestTM Pump System), where vaccines were initially solubilized in Triton X-100 to promote filtration. Next, membranes are incubated in TSB and FTM. These use two types of culture medium, tryptic soy broth (TSB) and fluid thioglycollate medium (FTM), with incubation times of 20-25ºC and 30-35ºC, respectively, to detect fungi, yeasts, and aerobic and anaerobic bacteria. The medium is incubated for 14 days, to enable the detection of slow-growth microorganisms that may be in a latent stage or weakened due to the extreme conditions of the production process (like the use of cleaning and disinfection agents, ultraviolet light, and preservers, for instance). All vaccines were effectively filtered in the SteritestTM Pump System. Membrane filtration and direct inoculation presented the same sensitivity to detect yeasts (0.1 CFU/mL) and anaerobic organisms (1 CFU/mL). For the detection of aerobic organisms, membrane filtration was 100 times more sensitive, compared to direct inoculation.Discussion: The specialized literature also reports that, apart from the higher sensitivity, membrane filtration affords to reduce contamination during the procedures, since it is carried out in a closed system. In addition, it is indicated in the analysis of large sample volumes. Moreover, membrane filtration reduces the occurrence of false positive results, since it removes the excess vaccine volume from the culture medium, which may be mistaken for turbidity caused by bacterial growth. In this sense, the membrane filtration technique is more appropriate in the control of vaccine sterility in foot-andmouth disease prevention strategies, and is an interesting tool to improve quality control of the product.

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