scholarly journals Effects of Urtica cannabina to Leymus chinensis Ratios on Ruminal Microorganisms and Fiber Degradation In Vitro

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 335
Author(s):  
Zhenbin Zhang ◽  
Shan Wang ◽  
Mengzhi Wang ◽  
Khuram Shahzad ◽  
Xiaoqing Zhang ◽  
...  
Keyword(s):  
Crude Protein ◽  
Culture Medium ◽  
Neutral Detergent Fiber ◽  
Leymus Chinensis ◽  
Ruminal Microorganisms ◽  
Fiber Degradation ◽  
Vessel Structure ◽  
Group A ◽  
C 50

The study was conducted in vitro to investigate the effects of different ratios of Urtica cannabina and Leymus chinensis on fiber microstructure and digestibility in ruminal fluid. The experiment was divided into five groups based on the U. cannabina/L. chinensis ratios: A (0:100), B (30:70), C (50:50), D (70:30), and E (100:0). The culture medium was collected at 0, 1, 3, 6, 12, and 24 h. The results showed that: (1) in vitro crude protein degradability (IVCPD) was higher in group A, whereas in vitro neutral detergent fiber degradability (IVNDFD) was higher in group C (p < 0.05); (2) protozoa count was increased from 1 h to 3 h and decreased afterwards, with significant differences observed in several genera (p < 0.05); (3) microbial crude protein (MCP) contents at 1, 3, 6, and 24 h were higher in groups A and C (p < 0.05); (4) the basic tissue of U. cannabina was gradually degraded. At 24h, the secondary xylem vessel structure was observed in groups B and C, but not in groups D and E. In summary, there was higher neutral detergent fiber (NDF) digestibility, higher rumen MCP contents, and lower protozoa count, showing the significance of the 50:50 ratio for microbial growth and fiber digestibility.

scholarly journals Evaluation of the Nutrient Composition, In Vitro Fermentation Characteristics, and In Situ Degradability of Amaranthus caudatus, Amaranthus cruentus, and Amaranthus hypochondriacus in Cattle

Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 18
Author(s):  
Kim Margarette C. Nogoy ◽  
Jia Yu ◽  
Young Gyu Song ◽  
Shida Li ◽  
Jong-Wook Chung ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Gas Production ◽  
Nutrient Composition ◽  
Neutral Detergent Fiber ◽  
Amaranthus Cruentus ◽  
Amaranthus Caudatus ◽  
Amaranthus Hypochondriacus

The amaranth plants showed high potential feed value as forage for ruminants. An in-depth study of this plant, particularly in cattle, will help extend its utilization as an alternative protein and fiber feed source in cattle feeding. In this study, the nutrient compositions of three different species of amaranth, Amaranthus caudatus L., Amaranthus cruentus L., and Amaranthus hypochondriacus L.—two varieties for each species, A.ca 74, A.ca 91, A.cu 62, A.cu 66, A. hy 30, and A. hy 48—were evaluated. The in vitro technique was used to evaluate the fermentation characteristics such as total gas production, total volatile fatty acids (VFA) concentration, pH, and ammonia concentration of the rumen fluid. Moreover, the effective degradabilities of dry matter (EDDM) and crude protein (EDCP) of the amaranth forages were determined through in situ bag technique. The amaranth forages: A. caudatus, A. cruentus, and A. hypochondriacus showed better nutritive value than the locally produced forages in Chungcheong province of Korea. The CP of the amaranth ranged from 11.95% to 14.19%, and the neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents ranged from 45.53% to 70.88% and 34.17% to 49.83%, respectively. Among the amaranth varieties, A. hypochondriacus 48 showed the most excellent ruminant feed nutrient quality (CP, 14.19%; NDF, 45.53%; and ADF, 34.17%). The effective degradabilities of dry matter (EDDM; 33–56%) and crude protein EDCP (27–59%) of the amaranth were lower compared to other studies, which could be due to the maturity stage at which the forages were harvested. Nonetheless, A. hypochondriacus 48 showed the highest EDDM (56.73%) and EDCP (59.09%). The different amaranth species did not differ greatly in terms of total VFA concentration or molar proportions, total gas production, or ammonia-N concentration. The high nutrient composition, and highly effective degradability of dry matter and crude protein, coupled with the favorable fermentation characteristics, suggest that the amaranth forages showed good to excellent feed quality for cattle.

Seasonal changes in forage nutritive value of common weeds encountered in Missouri pastures

2019 ◽  
Vol 34 (2) ◽  
pp. 164-171
Author(s):  
Gatlin Bunton ◽  
Zach Trower ◽  
Craig Roberts ◽  
Kevin W. Bradley
Keyword(s):  
Tall Fescue ◽  
Crude Protein ◽  
Nutritive Value ◽  
Late Summer ◽  
Growing Season ◽  
Quality Parameter ◽  
Neutral Detergent Fiber ◽  
Weed Species ◽  
Growing Seasons

AbstractDuring the 2015, 2016, and 2017 growing seasons, weed and weed-free mixed tall fescue and legume forage samples were harvested from 29 pastures throughout Missouri for investigation of the nutritive value of 20 common pasture weed species throughout the season. At certain times during the growing season, many broadleaf weed species had greater nutritive values for a given quality parameter as compared with the available weed-free, mixed tall fescue and legume forage harvested from the same location. There were no significant differences in crude protein concentration between the weed-free forage and many weeds throughout the growing season. However, crude protein content of common burdock, common cocklebur, common ragweed, dandelion, horsenettle, and lanceleaf ragweed was greater than that of the corresponding forage sample at multiple collection periods. The digestible neutral detergent fiber (dNDF) content of all broadleaf weeds except lanceleaf ragweed was significantly lower than that of the weed-free forage at all collection periods. Conversely, large crabgrass had significantly greater digestible neutral detergent fiber levels than did the mixed tall fescue forage at all sampling dates. Dandelion and spiny amaranth had greater in vitro true digestibility (IVTD) content than did the forage for the entire growing season. Three perennial weeds—horsenettle, vervains, and late boneset—did not differ in IVTD levels as compared with the mixed tall fescue and legume forage at any collection date. For most summer annual weeds, the trend was toward greater digestibility earlier in the season, with a gradual decline and often lower IVTD by the late summer or early fall. The results of this study will enable producers to make more informed management decisions about the potential benefit or detriment a weed may provide to the overall nutritive value of the pasture system.

scholarly journals Chemical characteristics, aerobic stability, and microbiological counts in corn silage re-ensiled with bacterial inoculant

2018 ◽  
Vol 53 (9) ◽  
pp. 1045-1052
Author(s):  
Mateus Merlo Coelho ◽  
Lúcio Carlos Gonçalves ◽  
José Avelino Santos Rodrigues ◽  
Kelly Moura Keller ◽  
Gustavo Vinícius de Souza dos Anjos ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Nutritive Value ◽  
Corn Silage ◽  
Neutral Detergent Fiber ◽  
Dry Matter Digestibility ◽  
Aerobic Stability ◽  
Whole Plant

Abstract: The objective of this work was to evaluate the effects of re-ensiling and bacterial inoculation on the quality of corn silage. The experiment was carried out in a 2x2 factorial design with or without inoculant (association of Lactobacillus plantarum and Propionibacterium acidipropionici), and with re-ensiling after 36 hours of aerobic exposure or only ensiling of the whole plant of 'BRS 1055' corn. The fermentative quality, nutritional parameters, dry matter losses, aerobic stability, and microbiological counts of silages were evaluated. Re-ensiling caused an increase of pH and in acetic acid and propionic acid concentrations, as well as in the dry matter (DM), crude protein, neutral detergent fiber, and neutral detergent fiber crude protein contents. Conversely, there was a reduction in the nonfiber carbohydrates concentration and in in vitro dry matter digestibility for the re-ensiled material. All changes were explained by the higher-effluent production and DM loss of re-ensiled material that was subjected to two compactions. Microbiology was not altered by the treatments. The use of inoculant altered ash content, but it did not influence other parameters. In contrast, re-ensiling after 36 hours of aerobic exposure caused a reduction in the nutritive value of corn silage and accentuated the DM losses.

YIELD, QUALITY AND PRESERVATION OF MOIST HAY SUBJECTED TO RAIN-FREE AND WEATHERED CONDITIONS

1990 ◽  
Vol 70 (2) ◽  
pp. 611-622
Author(s):  
V. S. BARON ◽  
G. W. MATHISON
Keyword(s):  
Propionic Acid ◽  
Crude Protein ◽  
Neutral Detergent Fiber ◽  
Moisture Level ◽  
Digestible Organic Matter ◽  
Medium Level ◽  
Large Round ◽  
Yield Quality ◽  
Medicago Sativa L

The effects of moisture level and a neutralized propionate preservative on yield, quality and preservation of rain-free and weathered alfalfa (Medicago sativa L.) hay were studied in separate trials. Moisture levels of 252, 185 and 138 g kg−1 in rain-free hay and 297, 211 and 180 g kg−1 in weathered hay were designated as High, Medium and Low, respectively. The weathered hay received 66 mm of rain at about 600 g kg−1 moisture and another 15 mm prior to baling at the Low moisture level. The preservative (680 g kg−1 propionic acid equivalent) was applied at 1.25 and 1.50% (wt wt−1) for rain-free and weathered hay, respectively. The High and Medium moisture level treatments had similar harvested dry matter (DM) yields in both rain-free and weathered hay. Rain-free hay DM yield at harvest was 6–9% higher for the High and Medium treatments than the Low moisture level, while for the weathered hay the Medium level was 3.0% higher (P < 0.05) than the Low moisture level. Poststorage forage qualities of the Low and Medium moisture level treatments were similar and higher than the High moisture treatment (P < 0.05) in rain-free hays, while the Medium level treatment was highest (P < 0.05) for forage quality in weathered hay. The Medium moisture level compared with Low moisture level resulted in 10.4, 10.6 and 15.8% higher yields of DM, in vitro digestible organic matter (IVDOM) and crude protein, respectively, in rain-free hay and 3.1, 6.3 and 5.7% higher, respectively, in weathered hay. The propionate was inconsistent in preventing DM loss during storage, but reduced temperature and mould count in both hay types. Application of 1.25 and 1.50% (wt wt−1) of propionate improved poststorage IVDOM by 2.0 and 3.6%; increased crude protein yield by 3.0 and 5.7% and IVDOM yield by 3.3 and 5.7%; reduced neutral detergent fiber by 5.4 and 7.6%; reduced acid detergent fiber by 5.4 and 7.1%; and reduced acid detergent insoluble nitrogen by 9 and 18% in rain-free and weathered hay, respectively. It is recommended that large round bales be harvested at moisture levels somewhat above 170 g kg−1 for both rain-free and weathered hay with or without a neutralized propionic acid preservative. Serious losses may occur above 250 g kg−1 moisture with or without the use of a preservative. Key words: Alfalfa, moist hay, neutralized propionate, large round bales, weathering, preservation

scholarly journals Effect of extruding on ruminal disappearance and lower gastrointestinal tract digestion of white lupin seeds

1993 ◽  
Vol 73 (3) ◽  
pp. 571-579 ◽  
Author(s):  
A. R. Kibelolaud ◽  
M. Vernay ◽  
C. Bayourthe ◽  
R. Moncoulon
Keyword(s):  
Gastrointestinal Tract ◽  
Crude Protein ◽  
Lupinus Albus ◽  
Neutral Detergent Fiber ◽  
White Lupin ◽  
Lower Gastrointestinal Tract ◽  
Rumen Degradation ◽  
Outflow Rate ◽  
Lupin Seeds

The effect of extruding white lupin (Lupinus albus 'Lublanc') seeds (WLS) at 110, 130, 150 or 180 °C on the in vitro solubility of crude protein (CP) and in sacco rumen degradation and intestinal digestion of rumen escape CP and fiber was determined. Rumen degradation was estimated by incubating nylon bags in the rumen of cows for 2, 4, 8, 16, 24 and 48 h. Extruding WLS at 110, 130, 150 and 180 °C reduced the CP-solubility by 32.8, 47.7, 58.4 and 67.5%, respectively. The effective ruminal degradabilities of CP, acid detergent fiber (ADF) and neutral detergent fiber (NDF) were evaluated assuming a ruminal outflow rate of 0.06 h−1. Heating WLS at 110, 130, 150 and 180 °C decreased the ruminal degradability of CP value: 89.8, 79.9, 65.1, 61.8 vs. 93.4% (raw), respectively; the corresponding values for ADF and NDF were: 38.4, 35.3, 34.2, 27.6 vs. 43.6% (raw) and 37.7, 33.1, 32.2, 26.5 vs. 39.4% (raw). Postruminal digestion was estimated using a sequence of ruminal in situ incubation for 4, 8 and 16 h, in vitro incubation in an acid-pepsin bath for 3 h and a mobile nylon bag technique distal to the abomasum. Extrusion of WLS decreased the degradability of CP, ADF and NDF in the rumen with a corresponding increase in the amounts digested in the postruminal sections; the whole-tract digestibility was generally unchanged. Therefore, the processing shifted the digestion of these components from rumen to the lower gastrointestinal tract. Key words: White lupin seed, in vitro solubility, rumen degradability, postruminal digestion, crude protein, detergent fiber

scholarly journals In Vitro Stimulation of Forage Fiber Degradation by Ruminal Microorganisms with Aspergillus oryzae Fermentation Extract

1993 ◽  
Vol 59 (10) ◽  
pp. 3171-3176 ◽  
Author(s):  
Vincent H. Varel ◽  
Kelly K. Kreikemeier ◽  
Hans-Joachim G. Jung ◽  
Ronald D. Hatfield
Keyword(s):  
Aspergillus Oryzae ◽  
Ruminal Microorganisms ◽  
Fiber Degradation ◽  
Stimulation Of

scholarly journals Animal performance and nutritional characteristics of Piatã-grass in integrated systems

2020 ◽  
Vol 72 (3) ◽  
pp. 1027-1033
Author(s):  
D.C. Martins ◽  
S.D.J. Villela ◽  
R.G. Almeida ◽  
S.A.C. Araújo ◽  
L.D. Silva ◽  
...  
Keyword(s):  
Organic Matter ◽  
Crude Protein ◽  
Nutritive Value ◽  
Soil Cover ◽  
Neutral Detergent Fiber ◽  
Integrated Systems ◽  
Animal Performance ◽  
Brachiaria Brizantha ◽  
Nutritional Characteristics

ABSTRACT The objective was to evaluate animal performance and nutritional characteristics of Brachiaria brizantha cv. BRS Piatã in two integrated systems, during the summer and winter, five years after the initial establishment of the area. The randomized block experimental design was adopted with treatments consisting of a control (five native trees per hectare) and an integrated crop-livestock-forestry (ICLF) system with 227 eucalyptus trees per hectare. Animal performance, canopy height, soil cover, forage mass (leaf, stem and senescent material), and forage nutritive value were evaluated. Differences between systems were observed in forage mass (total, leaf, stem and senescent material), soil cover and forage nutritive value in both seasons. Shading conditions provided by eucalyptus in the ICLF system lead to a reduction in forage mass and neutral detergent fiber content, and to an increase in crude protein and in vitro organic matter digestibility. However, no effect on animal performance was obtained. The presence of trees improves the forage nutritive value without impacting animal performance in integrated systems.

scholarly journals Effect of propionic and lactic acids on in vitro ruminal bacteria growth

2011 ◽  
Vol 40 (5) ◽  
pp. 1121-1127 ◽  
Author(s):  
Juliana Silva de Oliveira ◽  
Augusto César de Queiroz ◽  
Hilário Cuquetto Mantovani ◽  
Marcelo Rodrigues de Melo ◽  
Edenio Detmann ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Propionic Acid ◽  
Culture Medium ◽  
Lag Phase ◽  
Ruminal Bacteria ◽  
Bacteria Growth ◽  
Ruminal Microorganisms ◽  
The Media ◽  
Lactic Acids

The objective of this work was to evaluate the effect of the levels of lactic and propionic acids on in vitro fermentation of ruminal microorganisms. In experiment 1, the levels, in a total of 12 were the following: addition of 0 (control 1), 50, 100, 150, 200 and 250 mM of lactic acid and 0 (control 2), 50, 100, 150, 200 and 250 mM of propionic acid, respectively, in incubation flasks, which contained ruminal inoculum, glucose and synthetic culture medium, with two repetitions for each combination. In experiment 2, the combinations, in a total of 4, were the following: presence of 12 and 24 mM of propionic acid and 0 mg of glucose, respectively; presence of 12 and 24 mM of propionic acid and 40 mg of glucose, respectively, to the incubation flasks which contained ruminal inoculum, with or without glucose and in synthetic culture medium with two repetitions each. There was no effect on the specific growth velocity of ruminal microorganisms in the presence of lactic acid or propionic acid. However, when there were greater concentrations of these acids in the media, there was a longer lag phase in the microorganism phase. Acid propionic at the concentration of 24 mM inhibited the production of acid acetic and butyric acid in a media with glucose. Despite of not being used as a source of energy by the ruminal microorganisms, propionic acid affects their metabolism. Lactic and propionic acids inhibit growth of some ruminal microorganisms at elevated concentrations.

scholarly journals In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4507
Author(s):  
Luiz Juliano Valério Geron ◽  
Fabiana Gomes da Costa ◽  
Silvia Cristina de Aguiar ◽  
Jocilaine Garcia ◽  
Matheus Gonçalves Ribeiro ◽  
...  
Keyword(s):  
Crude Protein ◽  
Ammonia Nitrogen ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
Ruminal Fluid ◽  
Ph Values

<p>This study evaluated the digestibility of nutrients by, and parameters associated with, <em>in vitro </em>fermentation using different inocula (sheep ruminal fluid and feces) as well as the <em>in vivo </em>digestibility in sheep that were fed rations with 50% concentrate containing either no (0%) residue from the extraction of tamarind pulp (RETP) or 15% RETP. To determine the <em>in vitro </em>digestibility (<em>IV</em>D) of nutrients, two sheep, weighing 40.38 ± 2.10 kg, were used as inoculum donors. To determine the <em>in vivo </em>digestibility of nutrients, we used four sheep and a 3×2 factorial experimental design, with three methods of digestion of nutrients and two experimental rations (0% and 15% RETP). The variables were subjected to analysis of variance and the variables that showed differences at 5% probability were further analyzed using the Tukey test at 5% significance. The <em>IV</em>D using different inocula did not significantly differ (p&gt;0.05) from the <em>in vivo </em>digestibility in sheep for dry matter (DM), organic matter (OM), and neutral detergent fiber (NDF). The different methods for determining nutrient digestibility did not affect (p&gt;0.05) the digestibility of DM, OM, crude protein (CP), NDF, and acid detergent fiber (ADF) in rations with 0% and 15% RETP. However, the <em>IV</em>D of CP for rations containing 0% and 15% RETP incubated with both inocula was lower (p&lt;0.05) than the CP digestibility <em>in vivo</em>. The <em>in vivo </em>digestibility of ADF for rations containing 0% and 15% RETP was higher (P&lt;0.05) than the <em>IV</em>D using sheep ruminal fluid and feces as inocula. The pH values and concentration of ammonia nitrogen (NH3-N) after <em>in vitro </em>incubation for 24 h and the <em>in vivo </em>assay were not different (p&gt;0.05) for the rations containing 0% and 15% RETP, but the pH and NH3-N of both fermented and rumen contents differed (p&lt;0.05) depending on the inocula used and the <em>in vivo </em>assay. In summary, the digestibility of DM, OM, and NDF can be determined by the <em>in vitro </em>fermentation method using the ruminal fluid or feces of sheep as inocula in rations containing 0% or 15% RETP. However, <em>in vitro </em>fermentation is not a suitable method for the determination of pH and NH3-N concentration.</p>

scholarly journals High Fiber Cakes from Mediterranean Multipurpose Oilseeds as Protein Sources for Ruminants

Animals ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 918 ◽  
Author(s):  
Francesco Serrapica ◽  
Felicia Masucci ◽  
Emiliano Raffrenato ◽  
Maura Sannino ◽  
Alessandro Vastolo ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Protein Digestibility ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Ether Extract ◽  
Protein Supplements ◽  
High Fiber ◽  
Alternative Protein

Fifteen oilseed cakes from sunflower, pomegranate, cardoon, tobacco and hemp were characterized with regard to chemical composition, Cornell Net Carbohydrate and Protein System (CNCPS) fractionation, in vitro digestibility of dry matter, neutral detergent fiber, and crude protein. All the cakes presented low moisture, rather variable ether extract contents and medium to high levels of crude protein and neutral detergent fiber. The cakes significantly differed in terms of CNCPS partitioning and in vitro digestibility. Tobacco and hemp cakes presented high contents of slow degradable fractions of crude protein and carbohydrate joined to good post-ruminal protein digestibility. Cardoon cakes presented the highest rumen protein degradability. Based on crude protein content and intestinal digestibility of rumen undegraded protein, cakes of tobacco and hemp showed the better potential as alternative protein supplements for ruminants, while pomegranate appears to be the least suitable for ruminant feeding.

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