scholarly journals Can Enterocin M in Combination with Sage Extract Have Beneficial Effect on Microbiota, Blood Biochemistry, Phagocytic Activity and Jejunal Morphometry in Broiler Rabbits?

Animals ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 115 ◽  
Author(s):  
Monika Pogány Simonová ◽  
Ľubica Chrastinová ◽  
Anna Kandričáková ◽  
Soňa Gancarčíková ◽  
Eva Bino ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Phagocytic Activity ◽  
Ldl Cholesterol ◽  
Dietary Supplementation ◽  
Blood Biochemistry ◽  
Control Group ◽  
Gut Microflora ◽  
Enterocin M ◽  
And Control ◽  
Coagulase Positive Staphylococci

The present study investigated the effects of enterocin (Ent) M and sage extract alone and also in combination on the gut microflora, phagocytic activity, blood biochemistry, and morphometry of rabbits. Sixty-four rabbits (aged five weeks, M91 meatline, both sexes) were divided into three experimental groups: E (EntM; 50 µL/animal/day), S (sage; 10 µL/animal/day), and E + S (EntM + sage) groups and control group (C). The additives were administered in drinking water for a period of 21 days. Dietary supplementation of EntM and sage significantly reduced the coliforms (E: p < 0.001; S: p < 0.001; E + S: p < 0.001) in feces, while simultaneous addition of EntM and sage decreased enterococci (E + S: p < 0.0001), lactic acid bacteria (E + S: p < 0.01), and coagulase-positive staphylococci (E + S: p < 0.0001) in the appendix. Sage addition reduced HDL (S: p < 0.001) and LDL cholesterol (S: p < 0.001; E + S: p < 0.001), LDL/HDL ratio (S: p < 0.001; E + S: p < 0.01), and increased urea (S: p < 0.01; E + S: p < 0.001) and creatinine (S: p < 0.001; E + S: p < 0.001) in serum. EntM and sage application, alone or in combination, improve the jejunal morphometry (p < 0.0001) in rabbits.

scholarly journals Comparative Study of Vitamin C on Serum Lipid Profile in Healthy Male and Female Human Subjects

2012 ◽  
Vol 4 (3) ◽  
pp. 775-781 ◽  
Author(s):  
G. S. Gaur ◽  
A. K. Dixit
Keyword(s):  
Vitamin C ◽  
Lipid Profile ◽  
Ldl Cholesterol ◽  
Human Subjects ◽  
Test Group ◽  
Control Group ◽  
Blood Samples ◽  
Male And Female ◽  
Vitamin C Supplementation ◽  
And Control

This study aims to assess the comparative effects of vitamin C supplementation on lipid profiles in male and female human subjects. A total of 60 healthy individuals (male and female) were selected randomly, instructed and given the understanding of the purpose of study. The test group comprising  30 individuals  were given 500mg vitamin C tablets one daily for 30 days and control group of 30 individuals were given placebo capsules(glucose 500mg)  one daily for 30 days. Fasting blood samples were collected in the morning for estimation of cholesterol, triglycerides, HDL-C, LDL-C and VLDL-C on first day of the commencement of the study and second blood samples were taken after thirty days of supplementation and same estimations were carried out. Vitamin C caused reduction in serum total cholesterol and LDL cholesterol significantly but it did not have any statistically significant effect on HDL-C, VLDL-C and triglycerides. As far as gender is concerned the effect of vitamin C on lipid profile in males was not significantly different from those in females.© 2012 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi: http://dx.doi.org/10.3329/jsr.v4i3.8894 J. Sci. Res. 4 (3), 775-781 (2012)

Production Performance, Hematology and Serum Biochemistry of Commercial Broilers Supplemented with Nano Selenium and other Anti-Stressors during Summer

2020 ◽  
Author(s):  
J. Jamima ◽  
P. Veeramani ◽  
K. Kumanan ◽  
P. Kanagaraju
Keyword(s):  
Dietary Supplementation ◽  
Basal Diet ◽  
Serum Biochemistry ◽  
Production Performance ◽  
Feed Consumption ◽  
Control Group ◽  
Inorganic Selenium ◽  
Summer Stress ◽  
Diet Control ◽  
And Control

The aim of this study is to determine the effect of dietary supplementation of nano selenium on production performance in commercial broilers during summer stress. Dietary nano selenium was compared with other anti-stressors such as inorganic selenium, Vitamin E, Vitamin C, Betaine and 1% Glutamine. A total of 420 commercial broilers were divided into seven treatments with three replicates of 20 birds each and were supplemented with basal diet (control), inorganic Se (0.30 mg/kg), nano Se (0.15 mg/kg), Vit E (250 mg/kg), Betaine (2g/kg), Glutamine (1%) and Vit C (250 mg/kg). The highest body weight (1945.17 ± 25.28) and the lowest feed consumption (2973.16 ± 41.25) with better FCR (1.56 ± 0.02) was observed in birds supplemented with nano Se than other anti-stressors and control group. It was concluded that supplementation of nano Se in broiler diet improved the productivity of commercial broilers during summer stress.

scholarly journals The effect of Leiber Beta-S (1,3-1,6-β-D-glucan) on the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes in calves

2014 ◽  
Vol 83 (4) ◽  
pp. 347-354 ◽  
Author(s):  
Roman Wójcik
Keyword(s):  
Escherichia Coli ◽  
Oxidative Metabolism ◽  
Peripheral Blood ◽  
Phagocytic Activity ◽  
Mean Fluorescence Intensity ◽  
Jugular Vein ◽  
Dietary Supplementation ◽  
Control Group ◽  
E Coli ◽  
Experimental Group

The effect of the Leiber Beta-S (1,3-1,6-β-D-glucan) dietary supplement on the phagocytic activity (Phagotest) and oxidative metabolism (Phagoburst) of peripheral blood granulocytes and monocytes in calves was determined by flow cytometry. Fourteen animals were divided into two groups: a control group without dietary supplementation and an experimental group administered 50 mg/kg body weight/day of 1,3-1,6-β-D-glucan for 60 days. At the beginning of the experiment (day 0) and on days 15, 30 and 60, blood was sampled from the jugular vein to determine and compare immunological indicators. Leiber Beta-S significantly influenced (P ≤ 0.05, P ≤ 0.01, P ≤ 0.001) the percentages of phagocytic granulocytes (days 15 and 30) and monocytes (days 15, 30 and 60), and the percentage of bacteria engulfed by granulocytes (days 15 and 30) and monocytes (day 30). Leiber Beta-S increased the percentage of cells generating a respiratory burst in the population of granulocytes stimulated with fMLP (N-formyl-met-leu-phe) (day 15), PMA (4-phorbol-12-β-myristate-13-acetate) and Escherichia coli bacteria (days 15 and 30), and in the population of monocytes stimulated with fMLP (day 30), PMA (days 30 and 60) and E. coli (days 15, 30 and 60). The analyzed supplement increased mean fluorescence intensity in granulocytes stimulated with E. coli and fMLP (days 15 and 30) and PMA (days 30 and 60) as well as in monocytes stimulated with PMA, E. coli and fMLP (day 60). The findings of this study contribute to a better understanding of the effects of Leiber Beta-S on phagocytosis in calves, which have not been investigated to date.

scholarly journals Study of effectiveness of lactic acid at varroatosis in the apiaries of Tyumen Region, Russia

2020 ◽  
Vol 10 (5) ◽  
pp. 155-159
Author(s):  
T.F. Domatskaya ◽  
A.N. Domatsky ◽  
A.N. Domatsky
Keyword(s):  
Aqueous Solution ◽  
Lactic Acid ◽  
Negative Impact ◽  
Daily Basis ◽  
Control Group ◽  
Control Groups ◽  
Study Objective ◽  
And Control ◽  
The Impact ◽  
Bee Colonies

The study objective is to study the effectiveness of lactic acid in varroatosis and its impact on the development of bee colonies under conditions of the South of Tyumen region. The acaricidal effectiveness of lactic acid was studied in October 2019 using one method in two experiments in 30 broodless bee colonies kept in two apiaries, where bee colonies were divided into 2 groups (experimental - 10 colonies and control groups of 5 colonies each). For treatment of bee colonies from the experimental group, frames with bees were removed from the hives in turn, which were sprayed with a 15.0% aqueous solution of lactic acid in the volume of 5 ml on each side of the honeycomb. Bee colonies were treated twice with an interval of 5 days. The bees in the control group were not treated. Dead mites were registered on a daily basis in all groups within 5 days after each treatment, for these reasons laminated cardboard sheets were placed on the bottom of the hives, which then were taken out and the number of fallen ectoparasites was recorded. Studies on the impact of lactic acid on the development of bee colonies were conducted in 20 brood bee colonies in the period from May to July 2020. It has been found that the effectiveness of treatment of experimental bee colonies with the drug was 90.0 ± 2.1% (apiary No 1) and 7.8 ± 1.9% (apiary No 2). At the same time, the maximum death of mites in colonies was observed during the first day after treatment. In the control groups, the number of mites decreased by 10.9 ± 0.8% and 11.6 ± 1.0%, respectively. It has been established that the treatment of bees with an aqueous solution of lactic acid in the above concentration, dose and multiplicity did not have a negative impact on the development of bee colonies, their strength and food supply, and no deaths of bees and queens were detected during the observation period.

Blood plasma substrates and muscle lactic-acid response after exhaustive exercise in common carp and trout: indications for a limited lactate-shuttle

2004 ◽  
Vol 54 (2) ◽  
pp. 119-130 ◽  
Author(s):  
Tinka Murk ◽  
Paul Balm ◽  
Ron Boot ◽  
Guido van den Thillart ◽  
Vincent van Ginneken
Keyword(s):  
Lactic Acid ◽  
Blood Plasma ◽  
Fish Species ◽  
Strenuous Exercise ◽  
Control Group ◽  
Plasma Sodium ◽  
Lactate Shuttle ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Muscle Compartment ◽  
And Control

AbstractIn a Blazka swim tunnel swim trials with individual carp (Cyprinus carpio , N = 6, approximately 176 g) and rainbow trout (Oncorhynchus mykiss , N = 6 approximately 123 g) were performed until exhaustion at six body lengths per second (BL/s). Control carp (N = 6) and control trout (N = 6) were sampled after a moderate swim exercise at 1.5 BL/s. Significant differences were demonstrated in the exhausted carp group in comparison with the control group for plasma sodium (108.8%), lactic acid in red muscle (RM) (375.5%), lactic acid in white muscle (WM) (484.5%), triglycerids (133.9%), total protein (126.7%) and phospholipids (116.8%). In trout only, potassium was significantly elevated in the exhausted group (129.2%). T3- and T4-plasma values, as well as the T3/T4 ratio, were unaffected by the exercise protocol in both fish species. Despite the high lactic acid values in muscle tissue (RM: range 5-7 mM, WM: range 4-9 mM) in the exhausted groups of both fish species, the lactate in blood plasma in both fish species was not elevated (range 1.5-1.6 mM). This indicates that lactate is not released from the muscle compartment towards the blood and led to the concept of a 'non-release' lactic acid mechanism in cyprinid and a salmonid fish species after strenuous exercise.

scholarly journals Effect of Enterococcus faecium AL41 (CCCM8558) and Its Enterocin M on the Physicochemical Properties and Mineral Content of Rabbit Meat

Agriculture ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1045
Author(s):  
Monika Pogány Simonová ◽  
Ľubica Chrastinová ◽  
Andrea Lauková
Keyword(s):  
Enterococcus Faecium ◽  
Mineral Content ◽  
Negative Influence ◽  
Control Group ◽  
Research Activity ◽  
Natural Substances ◽  
Enterocin M ◽  
Rabbit Meat ◽  
Zinc Levels ◽  
And Control

Improving rabbit meat quality using natural substances has become an area of research activity in rabbit nutrition due to stabilization of husbandry health and economy. The present study evaluates the effect of bacteriocin-producing, beneficial strain Enterococcus faecium AL41/CCM8558 and its enterocin M (EntM) on the quality and mineral content of rabbit meat. Seventy-two Hycole rabbits (aged 35 days) were divided into EG1 (CCM8558 strain; 1.0 × 109 CFU/mL; 500µL/animal/d), EG2 (EntM; 50µL/animal/d), and control group (CG). The additives were administrated in drinking water for 21 days. Significant increase in meat phosphorus (EG1: p < 0.05; EG2: p < 0.0001) and iron (EG1, EG2: p < 0.001) contents was noted; sodium and zinc levels were only slightly higher in experimental groups compared with control data. The calcium (EG1, EG2: p < 0.001), potassium, and copper (EG1: p < 0.01) concentrations were reduced. The treatment did not have a negative influence on physicochemical traits of rabbit meat. Based on these results, we conclude that diet supplementation with beneficial strain E. faecium CCM8558 and its EntM could enhance the quality and mineral content of rabbit meat, with the focus on its iron and phosphorus contents.

scholarly journals The contribution of gut microflora to paracetamol metabolism

2014 ◽  
Vol 66 (1) ◽  
pp. 75-78
Author(s):  
Gordana Bojic ◽  
Vesna Milosevic ◽  
Vera Gusman ◽  
Anika Povazan ◽  
Maja Stojancevic
Keyword(s):  
Saline Solution ◽  
White Male ◽  
Great Influence ◽  
Control Group ◽  
Gut Microflora ◽  
Laboratory Mice ◽  
Enzymatic Systems ◽  
And Control ◽  
Paracetamol Metabolism

During the last decade, the important role of gut microflora as a special organ of the gastrointestinal system in the metabolism of drugs is well known. The aim of this study was to evaluate the role of the gut microecological population with enzymatic systems, especially beta-lyase, in the metabolism of paracetamol in mice. Two groups of 20 white male laboratory mice BALB/c, body weight 32+/-1.5 kg, were treated orally with neomycin sulphate (500 mg/kg in saline solution) and saline solution (10 ml/kg) twice daily for three days. After the treatment, the animals were given paracetamol dissolved in saline solution (200 mg/kg) intraperitoneally. The total amount of excreted paracetamol in 8 hours? collected urine was unchanged. A difference between treated and control mice was observed regarding a highly significant reduction in the excretion of 3-methylthiometabolites. A decrease in the excretion of thiomethyl metabolites was found in the control group compared to the experimental mice. Gut microflora had a great influence on the formation of metabolic precursors, thiomethyl-conjugates, and their oxidabile products. It is obvious that the ecosystem of gut microflora has an important role in the metabolism of paracetamol resulting in a significant reduction in the excretion of 3-methylthioparacetamol by urine, the glucuronide and sulphate.

scholarly journals The effect of propylthiouracil on function of phagocytic peripheral blood cells in persons with thyroid hyperfunction

2006 ◽  
Vol 134 (7-8) ◽  
pp. 290-294
Author(s):  
Aleksandar Djukic ◽  
Snezana Zivancevic-Simonovic ◽  
Predrag Djurdjevic ◽  
Nebojsa Arsenijevic
Keyword(s):  
Phagocytic Activity ◽  
Neutral Red ◽  
Mononuclear Phagocytes ◽  
Control Group ◽  
Polymorphonuclear Leucocytes ◽  
Mononuclear Leucocytes ◽  
And Control ◽  
Immunological System ◽  
System Objective ◽  
Healthy Persons

Introduction. It is known that hyperthyroidism as well as thyrosuppressive therapy can influence the cells of immunological system. Objective. To examine the function of phagocyte cells in persons with hyperthyroidism and to examine if propylthiouracil (PTU) influences this function. Method. The study included 15 patients with hyperthyroidism and 10 healthy persons. The parameters of phagocytic activity of mononuclear and polymorphonuclear leucocytes were tested by method of ingestion of particles of inactivated yeast labeled with neutral-red. Results. It was demonstrated that patients with hyperthyroidism, before the onset of therapy as well as 14 days after introduction of PTU, had decreased number of leucocytes (before PTU: 6.7?3.2?109/l, after PTU: 6.1?2.0?109/l and control: 8.0?1.6?109/l; p=0.039), PMN leucocytes (before PTU: 3.9?2,4 ?109/l, after PTU: 3.5?1.6?109/l and control: 4.8?0.9?109/l; p=0.037) and number of phagocyte PMN cells (before PTU: 0.9?0.9?109/l, after PTU: 0.9?0.7?109/l and control: 1.3?0.6 ?109/l; p<0,05), but they had increased index of phagocytosis (before PTU: 2.0?0.2, after PTU: 1.9?0.2 and control: 1.7?0.2; p=0.029), while capacity of phagocytosis remained unchanged (before PTU: 1.9?1.7?109/l, after PTU: 1.6?1.9?109/l and control: 2.4?1.4?109/l; p>0.05). The number of mononuclear leucocytes and parameters of phagocytic activity of mononuclear phagocytes in persons with hyperthyroidism did not change significantly in comparison with the control group. Conclusion. Patients with hyperthyroidism had decreased number of leucocytes, PMN leucocytes and number of phagocyte PMN cells, and increased index of phagocytosis, while capacity of phagocytosis remained unchanged. The number and parameters of phagocytic activity of mononuclear leucocytes did not change. PTU therapy had no effect on the examined parameters.

scholarly journals Persistence of orally administered Megasphaera elsdenii and Ruminococcus bromii in the rumen of beef cattle fed a high grain (barley) diet

2012 ◽  
Vol 52 (5) ◽  
pp. 297 ◽  
Author(s):  
Athol V. Klieve ◽  
Stuart R. McLennan ◽  
Diane Ouwerkerk
Keyword(s):  
Lactic Acid ◽  
Dry Matter ◽  
Block Design ◽  
Bos Indicus ◽  
High Growth ◽  
Control Group ◽  
Control Groups ◽  
Rapid Production ◽  
Megasphaera Elsdenii ◽  
And Control

When cattle are fed grain, acidotic ruminal conditions and decreased efficiency in starch utilisation can result from the rapid production and accumulation of lactic acid in the rumen. The efficacy of drenching cattle with Megasphaera elsdenii and Ruminococcus bromii to improve animal performance was investigated. A feedlot trial was undertaken with 80 Bos indicus crossbred steers (initial liveweight 347.1 (s.d. 31.7) kg) in 10 pens in a randomised complete block design. An empty-pen-buffer was maintained between treated (inoculated) and untreated (control) groups to avoid transfer of inoculant bacteria to the control steers. Inoculated steers were orally drenched with M. elsdenii YE34 and R. bromii YE282, and populations increased rapidly over 3–14 days. The steers were fed for a total of 70 days with commercial, barley-based, feedlot rations. High growth rates (1.91 kg per day) were achieved throughout the experiment in both the inoculated and control steers. Intakes averaged 21.3 g dry matter (DM) per kg liveweight per day. There was probably no acidosis achieved in this trial following challenge (i.e. no change in pH occurred). There were no differences in any production or carcass measurements between the control and inoculated steers overall. However, the control group acquired dense ruminal populations of M. elsdenii by Day 14, while R. bromii populations established at high densities within the first 2 weeks but then declined and were undetectable by Day 50. R. bromii appears to be only transiently dominant, and once its dominance waned, it appeared that Ruminobacter spp. established in the rumen. Ruminobacter spp. became dominant between 14 and 28 days in all the steers examined and persisted through to the end of the study. These Ruminobacter spp. may be of future interest in the development of probiotics for grain-fed cattle.

scholarly journals Benefficial effect of plant extracts in rabbit husbandry

2012 ◽  
Vol 81 (3) ◽  
pp. 245-250 ◽  
Author(s):  
Renáta Szabóová ◽  
Andrea Lauková ◽  
Ľubica Chrastinová ◽  
Viola Strompfová ◽  
Monika Pogány Simonová ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Control Group ◽  
Feed Conversion ◽  
Prophylactic Measure ◽  
Natural Substances ◽  
Group 2 ◽  
And Control ◽  
Coagulase Positive Staphylococci ◽  
Experimental Group ◽  
Group 1

The present study evaluates the effect of plant extracts of oregano and commercial XtraktTM administrations on microbial, biochemical, immunological and nutritional indicators and on Eimeria sp. oocyst occurrence in rabbits. Rabbits (5 weeks old, Hy-plus hybrid, n = 66) were divided into experimental group 1 (E1) with oregano extract application, experimental group 2 (E2) with XtractTM application and control group. Natural substances were administered for the first 21 days. The experiment lasted for 42 days. The antibacterial effect was determined by the decrease of coagulase-positive staphylococci in E1 compared to control at day 42. Staphylococcus aureus cells were detected in lower counts in E2 compared to control at day 21. The counts of Clostridium-like bacteria were lower in both experimental groups at day 21 compared to day 7 (difference 1.2 and 1.3 log cycles, respectively) and to control (difference 0.5 and 0.3 log cycles, respectively). At day 7, the counts of coliforms in E1 were significantly lower than in E2 (P < 0.01). In rabbits fed with oregano, reduction of Eimeria sp. oocysts and higher value of phagocytic activity (21.6 ± 0.51 %) were found compared to XtractTM, and prolonged immuno-stimulatory effect was noted. In the animals of both experimental groups higher final weight, feed conversion ratio and lower mortality were achieved compared to control. The administration of oregano showed antibacterial, anticoccidial, and immunomodulatory effects. The results showed that oregano administration may be used as an alternative prophylactic measure in rabbits.

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