scholarly journals Comparative Evaluation of Various Total Antioxidant Capacity Assays Applied to Phenolic Compounds with the CUPRAC Assay

Molecules ◽  
2007 ◽  
Vol 12 (7) ◽  
pp. 1496-1547 ◽  
Author(s):  
Reşat Apak ◽  
Kubilay Güçlü ◽  
Birsen Demirata ◽  
Mustafa Özyürek ◽  
Saliha Çelik ◽  
...  
10.5219/1025 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 94-99
Author(s):  
Soňa Škrovánková ◽  
Lukáš Snopek ◽  
Jiří Mlček ◽  
Eva Volaříková

Honey contains important bioactive compounds (enzymes, phenolic compounds, vitamins, and minerals) with several positive health effects for humans. In the study six types of honey (acacia, rape, floral, multi flower, forest, and honeydew honeys), of Czech and Slovak origin, were evaluated for bioactive compounds by means of color, polyphenols and antioxidant capacity analyses. The brightest color of honeys, the lowest values measured spectometrically, had acacia and rape honeys, followed by floral, and darker multi flower and forest honeys, and honeydew honeys. Polyphenols (PP) amount, determined by spectrophotometric method with Folin-Ciocalteu reagent, was highest for the darkest honeydew honeys, followed by multi flower and forest honey, brighter floral honeys, and rape and acacia honey. Honeys polyphenols were in the range from 54.0 to 254.2 mg GAE.100g-1. The total antioxidant capacity (TAC) was analyzed by spectrometric methods with ABTS and DPPH reagents. Antioxidant capacity values are in agreement with the PP contents order. They were highest also for honeydew honeys (59.2 - 89.6 and 73.1 - 118.7 mg TE.100g-1), followed by multi flower (66.0 and 56.7 mg TE.100g-1) and forest honey (56.0 and 49.1 mg TE.100g-1), then floral honeys (33.0 - 49.2 and 27.8 - 38.7 mg TE.100g-1) and the lowest values for rape (19.0 and 28.1 mg TE.100g-1) and acacia (15.5 and 11.3 mg TE.100g-1) honey. A positive correlation between color, PP amount and TAC was evaluated for analyzed honeys. Darker honey samples showed higher values of phenolic compounds and antioxidant potential, therefore they belong to the honey types with higher amount of bioactive compounds such as antioxidants. 


2020 ◽  
Vol 323 ◽  
pp. 126829 ◽  
Author(s):  
Daniel Hinojosa-Nogueira ◽  
Sergio Pérez-Burillo ◽  
José Ángel Rufián-Henares ◽  
Silvia Pastoriza de la Cueva

2015 ◽  
Vol 16 (3) ◽  
pp. 234-237 ◽  
Author(s):  
Meenakshi Nankar ◽  
Hrishikesh Walimbe ◽  
Mohammed Nadeem Ahmed Bijle ◽  
Sneha Muchandi ◽  
Srishti Chaturvedi ◽  
...  

ABSTRACT Background Dental caries is a major problem in preschool children. The contribution of saliva in providing defense during caries process is of primary importance. pH buffer capacity through bicarbonate, phosphate and protein buffer systems have universal acceptance as a caries defense mechanism. Antioxidant capacity of saliva can constitute a first line of defense against chronic degenerative diseases including dental caries. Till date, no study is presented with salivary antioxidant capacity of younger children affected with severe early childhood caries with its salivary pH correlation. Hence, this study was carried out to compare, evaluate and correlate the salivary total antioxidant capacity (TAC) and salivary pH of children with caries-free and severe early childhood caries. Materials and methods Fifty children from ages 3 to 5 years divided into two study groups had undergone screening. Group I (n = 25) with severe early childhood caries (S-ECC) and group II (n = 25) who were caries free. Unstimulated whole saliva of subjects were in the collection during the study by draining method. Salivary pH determination of saliva samples was done using pH indicator paper strips. The TAC was done using an antioxidant assay with the help of a spectrophotometer at wavelength 532 nm. The means of salivary pH and TAC were subjected to analysis using unpaired student ‘t’ test and correlation was determined using Pearsons correlation coefficient analysis. Results Mean salivary pH was higher in group II (7.46 ± 0.37). Mean TAC was greater in group I (1.82 ± 0.19). A statistically significant negative correlation as seen between TAC and salivary pH in S-ECC patients. Conclusion The study concludes that salivary TAC increases in patients with S-ECC are by that showing a high indirect relationship with salivary pH. How to cite this article Muchandi S, Walimbe H, Bijle MNA, Nankar M, Chaturvedi S, Karekar P. Comparative Evaluation and Correlation of Salivary Total Antioxidant Capacity and Salivary pH in Caries-free and Severe Early Childhood Caries Children. J Contemp Dent Pract 2015;16(3):234-237.


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