scholarly journals Characterization of Prodiginine Pathway in Marine Sponge-Associated Pseudoalteromonas sp. PPB1 in Hilo, Hawai‘i

2020 ◽  
Vol 4 ◽  
Author(s):  
Francis E. Sakai-Kawada ◽  
Courtney G. Ip ◽  
Kehau A. Hagiwara ◽  
Hoang-Yen X. Nguyen ◽  
Christopher-James A. V. Yakym ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Marine Sponge ◽  
Draft Genome ◽  
Marine Sponges ◽  
Consumer Products ◽  
Sponge Tissue ◽  
Tissue Samples ◽  
Biological Assays ◽  
Surface Sterilization ◽  
Host Sponge

Interest in bioactive pigments stems from their ecological role in adaptation, as well as their applications in various consumer products. The production of these bioactive pigments can be from a variety of biological sources, including simple microorganisms that may or may not be associated with a host. This study is particularly interested in the marine sponges, which have been known to harbor microorganisms that produce secondary metabolites like bioactive pigments. In this study, marine sponge tissue samples were collected from Puhi Bay off the Eastern shore of Hilo, Hawai‘i and subsequently were identified as Petrosia sp. with red pigmentation. Using surface sterilization and aseptic plating of sponge tissue samples, sponge-associated microorganisms were isolated. One isolate (PPB1) produced a colony with red pigmentation like that of Petrosia sp., suggesting an integral relationship between this particular isolate and the sponge of interest. 16S characterization and sequencing of PPB1 revealed that it belonged to the Pseudoalteromonas genus. Using various biological assays, both antimicrobial and antioxidant bioactivity was shown in Pseudoalteromonas sp. PPB1 crude extract. To further investigate the genetics of pigment production, a draft genome of PPB1 was sequenced, assembled, and annotated. This revealed a prodiginine biosynthetic pathway and the first cited-incidence of a prodiginine-producing Pseudoalteromonas species isolated from a marine sponge host. Further understanding into the bioactivity and biosynthesis of secondary metabolites like pigmented prodiginine may uncover the complex ecological interactions between host sponge and microorganism.

scholarly journals Microorganisms Associated with the Marine Sponge Scopalina hapalia: A Reservoir of Bioactive Molecules to Slow Down the Aging Process

2020 ◽  
Vol 8 (9) ◽  
pp. 1262
Author(s):  
Charifat Said Hassane ◽  
Mireille Fouillaud ◽  
Géraldine Le Goff ◽  
Aimilia D. Sklirou ◽  
Jean Bernard Boyer ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Marine Sponge ◽  
Marine Sponges ◽  
Sirtuin 1 ◽  
Bioactive Molecules ◽  
Aging Research ◽  
Fyn Kinase ◽  
The Indian Ocean ◽  
Metabolites Production ◽  
Future Work

Aging research aims at developing interventions that delay normal aging processes and some related pathologies. Recently, many compounds and extracts from natural products have been shown to delay aging and/or extend lifespan. Marine sponges and their associated microorganisms have been found to produce a wide variety of bioactive secondary metabolites; however, those from the Southwest of the Indian Ocean are much less studied, especially regarding anti-aging activities. In this study, the microbial diversity of the marine sponge Scopalina hapalia was investigated by metagenomic analysis. Twenty-six bacterial and two archaeal phyla were recovered from the sponge, of which the Proteobacteria phylum was the most abundant. In addition, thirty isolates from S. hapalia were selected and cultivated for identification and secondary metabolites production. The selected isolates were affiliated to the genera Bacillus, Micromonospora, Rhodoccocus, Salinispora, Aspergillus, Chaetomium, Nigrospora and unidentified genera related to the family Thermoactinomycetaceae. Crude extracts from selected microbial cultures were found to be active against seven targets i.e., elastase, tyrosinase, catalase, sirtuin 1, Cyclin-dependent kinase 7 (CDK7), Fyn kinase and proteasome. These results highlight the potential of microorganisms associated with a marine sponge from Mayotte to produce anti-aging compounds. Future work will focus on the isolation and the characterization of bioactive molecules.

scholarly journals Biological and Chemical Diversity of Marine Sponge-Derived Microorganisms over the Last Two Decades from 1998 to 2017

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 853 ◽  
Author(s):  
Mei-Mei Cheng ◽  
Xu-Li Tang ◽  
Yan-Ting Sun ◽  
Dong-Yang Song ◽  
Yu-Jing Cheng ◽  
...  
Keyword(s):  
Natural Products ◽  
Secondary Metabolites ◽  
Natural Product ◽  
Marine Sponge ◽  
Marine Sponges ◽  
Chemical Diversity ◽  
Comprehensive Overview ◽  
Bioactive Secondary Metabolites ◽  
The Relationship ◽  
Product Chemistry

Marine sponges are well known as rich sources of biologically natural products. Growing evidence indicates that sponges harbor a wealth of microorganisms in their bodies, which are likely to be the true producers of bioactive secondary metabolites. In order to promote the study of natural product chemistry and explore the relationship between microorganisms and their sponge hosts, in this review, we give a comprehensive overview of the structures, sources, and activities of the 774 new marine natural products from sponge-derived microorganisms described over the last two decades from 1998 to 2017.

scholarly journals Comparative Genomics Reveals Metabolic Specificity of Endozoicomonas Isolated from a Marine Sponge and the Genomic Repertoire for Host-Bacteria Symbioses

2019 ◽  
Vol 7 (12) ◽  
pp. 635
Author(s):  
Anoop Alex ◽  
Agostinho Antunes
Keyword(s):  
Comparative Genomics ◽  
Marine Sponge ◽  
Draft Genome ◽  
Carbon Sources ◽  
Gene Clusters ◽  
Marine Sponges ◽  
Symbiotic Association ◽  
Limited Information ◽  
Secretion Systems ◽  
Genomic Signatures

The most recently described bacterial members of the genus Endozoicomonas have been found in association with a wide variety of marine invertebrates. Despite their ubiquity in the host holobiont, limited information is available on the molecular genomic signatures of the symbiotic association of Endozoicomonas with marine sponges. Here, we generated a draft genome of Endozoicomonas sp. OPT23 isolated from the intertidal marine sponge Ophlitaspongia papilla and performed comprehensive comparative genomics analyses. Genome-specific analysis and metabolic pathway comparison of the members of the genus Endozoicomonas revealed the presence of gene clusters encoding for unique metabolic features, such as the utilization of carbon sources through lactate, L-rhamnose metabolism, and a phenylacetic acid degradation pathway in Endozoicomonas sp. OPT23. Moreover, the genome harbors genes encoding for eukaryotic-like proteins, such as ankyrin repeats, tetratricopeptide repeats, and Sel1 repeats, which likely facilitate sponge-bacterium attachment. The genome also encodes major secretion systems and homologs of effector molecules that seem to enable the sponge-associated bacterium to interact with the sponge and deliver the virulence factors for successful colonization. In conclusion, the genome analysis of Endozoicomonas sp. OPT23 revealed the presence of adaptive genomic signatures that might favor their symbiotic lifestyle within the sponge host.

scholarly journals Assessing the Diversity and Biomedical Potential of Microbes Associated With the Neptune’s Cup Sponge, Cliona patera

2021 ◽  
Vol 12 ◽  
Author(s):  
Xin Yi Ho ◽  
Nursheena Parveen Katermeran ◽  
Lindsey Kane Deignan ◽  
Ma Yadanar Phyo ◽  
Ji Fa Marshall Ong ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Temporal Stability ◽  
Marine Sponges ◽  
Molecular Networks ◽  
Sponge Tissue ◽  
Prokaryotic Community ◽  
Bacterial Strains ◽  
Tissue Samples ◽  
Bacterial Phyla ◽  
Organic Extracts

Marine sponges are known to host a complex microbial consortium that is essential to the health and resilience of these benthic invertebrates. These sponge-associated microbes are also an important source of therapeutic agents. The Neptune’s Cup sponge, Cliona patera, once believed to be extinct, was rediscovered off the southern coast of Singapore in 2011. The chance discovery of this sponge presented an opportunity to characterize the prokaryotic community of C. patera. Sponge tissue samples were collected from the inner cup, outer cup and stem of C. patera for 16S rRNA amplicon sequencing. C. patera hosted 5,222 distinct OTUs, spanning 26 bacterial phyla, and 74 bacterial classes. The bacterial phylum Proteobacteria, particularly classes Gammaproteobacteria and Alphaproteobacteria, dominated the sponge microbiome. Interestingly, the prokaryotic community structure differed significantly between the cup and stem of C. patera, suggesting that within C. patera there are distinct microenvironments. Moreover, the cup of C. patera had lower diversity and evenness as compared to the stem. Quorum sensing inhibitory (QSI) activities of selected sponge-associated marine bacteria were evaluated and their organic extracts profiled using the MS-based molecular networking platform. Of the 110 distinct marine bacterial strains isolated from sponge samples using culture-dependent methods, about 30% showed quorum sensing inhibitory activity. Preliminary identification of selected QSI active bacterial strains revealed that they belong mostly to classes Alphaproteobacteria and Bacilli. Annotation of the MS/MS molecular networkings of these QSI active organic extracts revealed diverse classes of natural products, including aromatic polyketides, siderophores, pyrrolidine derivatives, indole alkaloids, diketopiperazines, and pyrone derivatives. Moreover, potential novel compounds were detected in several strains as revealed by unique molecular families present in the molecular networks. Further research is required to determine the temporal stability of the microbiome of the host sponge, as well as mining of associated bacteria for novel QS inhibitors.

An Overview of Synthesis of Indole Alkaloids and Biological Activities of Secondary Metabolites Isolated from Hyrtios Species

2019 ◽  
Vol 19 (3) ◽  
pp. 194-205 ◽  
Author(s):  
M. Novanna ◽  
K.R. Ethiraj ◽  
S. Kannadasan
Keyword(s):  
Secondary Metabolites ◽  
Marine Sponge ◽  
Biological Activities ◽  
Indole Alkaloids ◽  
Marine Sponges ◽  
Review Article ◽  
Marine Organisms ◽  
Biologically Active ◽  
Synthetic Routes ◽  
Marine Natural Compounds

Marine sponges are a rich source of more than 50% of marine natural compounds that have been isolated from marine organisms. This review article is focused on the importance of biologically active and pharmaceutically important secondary metabolites extracted from one of the important classes of marine sponge Hyrtios sp. This review also deals with reported synthetic routes of some indole alkaloids extracted from the marine sponge Hyrtios sp. A range of bioactivities displayed by indole-based alkaloids is described.

scholarly journals Uji Daya Hambat Ekstrak Spons Laut Callyspongia aerizusa terhadap Pertumbuhan Bakteri Shigella dan Staphylococcus epidermidis

2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Tifany T. Korompis ◽  
Christi D. Mambo ◽  
Edward Nangoy
Keyword(s):  
Secondary Metabolites ◽  
Laboratory Study ◽  
Marine Sponge ◽  
Staphylococcus Epidermidis ◽  
Antibacterial Agents ◽  
Marine Sponges ◽  
Inhibition Activity ◽  
Experimental Laboratory ◽  
Inhibition Zones ◽  
Sponge Extract

Abstract: Callyspongia sp. is a kind of marine sponges that produces secondary metabolites, such as steroids, alkaloids, flavonoids, and terpenoids which can be used as antibacterial agents. This study was aimed to determine the inhibition activity of Callyspongia aerizusa marine sponge extract against the growth of Shigella and Staphylococcus epidermidis bacteria. This was an experimental laboratory study. Inhibition activity was tested by using the modified Kirby-Bauer method. The inhibition zones formed by the marine sponge extract were measured. The results showed that the inhibition zones of marine sponge extract against Shigella bacteria was 6.1 mm and against Staphylococcus epidermidis bacteria was 6.6 mm. Conclusion: Callyspongia aerizusa extract had moderate inhibition activity against the growth of Shigella and Staphylococcus epidermidis bacteria.Keywords: inhibition test, Callyspongia aerizusa, Shigella, Staphylococcus epidermidis Abstrak: Callyspongia sp. merupakan salah satu jenis spons laut yang menghasilkan metabolit sekunder berupa steroid, alkaloid, flavonoid, dan terpenoid yang bisa dimanfaatkan sebagai bahan antibakteri. Penelitian ini bertujuan untuk mengetahui ada tidaknya daya hambat dari ekstrak spons laut Callyspongia aerizusa terhadap pertumbuhan bakteri Shigella dan Staphylococcus epidermidis. Jenis penelitian ialah eksperimental laboratorik. Uji daya hambat dengan metode Kirby-Bauer yang dimodifikasi menggunakan sumuran untuk mengukur zona hambat yang terbentuk oleh ekstrak spons laut Callyspongia aerizusa. Hasil penelitian mendapatkan zona hambat yang terbentuk pada bakteri Shigella sebesar 6,1 mm dan pada bakteri Staphylococcus epidermidis sebesar 6,6 mm. Simpulan: Ekstrak spons laut Callyspongia aerizusa memiliki daya hambat sedang terhadap pertumbuhan bakteri Shigella dan Staphylococcus epidermidis.Kata kunci: uji daya hambat, Callyspongia aerizusa, Shigella, Staphylococcus epidermidis

scholarly journals Draft Genome Sequence of Marine Sponge Symbiont Pseudoalteromonas luteoviolacea IPB1, Isolated from Hilo, Hawaii

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Francis E. Sakai-Kawada ◽  
Christopher J. Yakym ◽  
Martin Helmkampf ◽  
Kehau Hagiwara ◽  
Courtney G. Ip ◽  
...  
Keyword(s):  
Secondary Metabolite ◽  
Genome Sequence ◽  
Marine Sponge ◽  
Genome Assembly ◽  
Draft Genome ◽  
Genome Mining ◽  
Biosynthetic Pathways ◽  
Ecological Interaction ◽  
Draft Genome Assembly ◽  
Host Sponge

We report here the 6.0-Mb draft genome assembly of Pseudoalteromonas luteoviolacea strain IPB1 that was isolated from the Hawaiian marine sponge Iotrochota protea . Genome mining complemented with bioassay studies will elucidate secondary metabolite biosynthetic pathways and will help explain the ecological interaction between host sponge and microorganism.

scholarly journals Isolation and genome sequencing of 14 Spongia sp. bacterial associates expands the taxonomic and functional breadth of the cultivatable marine sponge microbiome

2020 ◽  
Author(s):  
Elham Karimi ◽  
Rodrigo Costa
Keyword(s):  
Marine Sponge ◽  
Draft Genome ◽  
Gene Clusters ◽  
Marine Sponges ◽  
Microbial Consortia ◽  
Bacterial Strains ◽  
Signalling Molecules ◽  
Host Microbe Interactions ◽  
Taxonomic Groups ◽  
Coding Potential

AbstractMarine sponges live with complex microbial consortia, which have been considered as potential sources of novel natural products. However, the usual recalcitrance of host-associated microorganisms to cultivation makes studying sponge symbionts challenging. To tackle this complexity, exploration of cultivated sponge-associated bacteria and their coding potential is unavoidable. In this study, we isolate and report the draft genome sequences of 14 bacterial strains from the marine sponge Spongia sp. using R2A and VXA media. The strains belong to the classes Actinobacteria, Gammaproteobacteria, Alphaproteobacteria, and Cytophagia spanning 11 formally described genera plus two potentially novel genera in the Rhodobacteraceae family and one potentially novel family in the Cytophagales order. Functional genomics revealed presumed symbiosis factors typical of specific taxonomic groups (i.e. taurine metabolism genes among the Alphaproteobacteria, chitinase encoding genes and eukaryotic-like proteins in the Cytophagia genome) while multidrug efflux pumps, also important in host-microbe interactions, were common across all genomes. Moreover, we detected 76 secondary-metabolite biosynthetic gene clusters putatively involved in the production of drug-like compounds or signalling molecules across all genomes, warranting future biotechnologically driven research into their coding potential.

scholarly journals Elicited ROS Scavenging Activity, Photoprotective, and Wound-Healing Properties of Collagen-Derived Peptides from the Marine Sponge Chondrosia reniformis

Marine Drugs ◽  
2018 ◽  
Vol 16 (12) ◽  
pp. 465 ◽  
Author(s):  
Marina Pozzolini ◽  
Enrico Millo ◽  
Caterina Oliveri ◽  
Serena Mirata ◽  
Annalisa Salis ◽  
...  
Keyword(s):  
Wound Healing ◽  
Marine Sponge ◽  
Biological Effects ◽  
Marine Sponges ◽  
In Vitro Toxicity ◽  
Scavenging Activity ◽  
Ros Scavenging ◽  
General Chemical ◽  
Collagen Hydrolysates

Recently, the bioactive properties of marine collagen and marine collagen hydrolysates have been demonstrated. Although there is some literature assessing the general chemical features and biocompatibility of collagen extracts from marine sponges, no data are available on the biological effects of sponge collagen hydrolysates for biomedical and/or cosmetic purposes. Here, we studied the in vitro toxicity, antioxidant, wound-healing, and photoprotective properties of four HPLC-purified fractions of trypsin-digested collagen extracts—marine collagen hydrolysates (MCHs)—from the marine sponge C. reniformis. The results showed that the four MCHs have no degree of toxicity on the cell lines analyzed; conversely, they were able to stimulate cell growth. They showed a significant antioxidant activity both in cell-free assays as well as in H2O2 or quartz-stimulated macrophages, going from 23% to 60% of reactive oxygen species (ROS) scavenging activity for the four MCHs. Finally, an in vitro wound-healing test was performed with fibroblasts and keratinocytes, and the survival of both cells was evaluated after UV radiation. In both experiments, MCHs showed significant results, increasing the proliferation speed and protecting from UV-induced cell death. Overall, these data open the way to the use of C. reniformis MCHs in drug and cosmetic formulations for damaged or photoaged skin repair.

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