scholarly journals A Mapped Locus on LG A6 of Brassica juncea Line Tumida Conferring Resistance to White Rust Contains a CNL Type R Gene

2020 ◽  
Vol 10 ◽  
Author(s):  
Latika Bhayana ◽  
Kumar Paritosh ◽  
Heena Arora ◽  
Satish Kumar Yadava ◽  
Priyansha Singh ◽  
...  
Keyword(s):  
Brassica Juncea ◽  
R Gene ◽  
White Rust

scholarly journals Deciphering the Molecular Architecture of a Candidate R-gene (BjuWRR1) Product Mediating White Rust Resistance in Brassica juncea

2021 ◽  
Vol 12 (5) ◽  
pp. 393-401
Author(s):  
Chinmaya Kumar Das ◽  
◽  
Umasankar Nayak ◽  
Preetinanda Pati ◽  
Mihir Ranjan Mohanty ◽  
...  
Keyword(s):  
Brassica Juncea ◽  
Molecular Interaction ◽  
Rust Resistance ◽  
Hydrophobic Interactions ◽  
Coiled Coil ◽  
R Gene ◽  
Molecular Architecture ◽  
Three Dimensional Model ◽  
White Rust ◽  
Rust Pathogen

In this investigation, a three-dimensional model of a R-gene encoded product BjuWRR1 which is known to play a role in white rust resistance in Brassica juncea was developed to synthesize innovative ways for evolving white rust resistant cultivars. The model was built from the amino acid sequence of BjuWRR1 using structural template information of a disease resistance protein (RPP13-like protein 4 of Arabidopsis thaliana) with the help of homology-based modelling approach. Built models were validated for their stereochemical parameters and structural descriptors using Ramachandran plot analysis, protein structure analysis and ERRAT analysis. Structural analysis of BjuWRR1 model revealed that it is composed of three distinct domains namely a coiled-coil domain, a central NB-ARC nucleotide binding domain and a hypervariable leucine-rich repeat domain. Further, canonical conserved motifs such as P-loop, Kinase2-motif and HD-motif were found in the NB-ARC domain. The built model would help in understanding the molecular basis of plant-immunity against white rust pathogen by understanding the significance of inter-domain interactions in BuWRR1 in triggering the activation of downstream defense response against the white rust pathogen by promoting oligomerization of coiled-coil domains through stabilized hydrophobic interactions and interaction with NB-ARC domain. Presence of patches of charged residues in each domain of BjuWRR1 indicated their possible role in intra-molecular interaction with other domains. Therefore, this model can help in designing functional genomic studies to understand the role of intra-molecular interaction in BjuWRR1 to mediate resistance against white rust pathogen.

scholarly journals BjuWRR1, a CC-NB-LRR gene identified in Brassica juncea, confers resistance to white rust caused by Albugo Candida

2019 ◽  
Author(s):  
Heena Arora ◽  
K. Lakshmi Padmaja ◽  
Kumar Paritosh ◽  
Nitika Mukhi ◽  
A. K. Tewari ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Brassica Juncea ◽  
Gene Pool ◽  
Evolutionary Dynamics ◽  
Genomic Sequence ◽  
Oilseed Crop ◽  
R Gene ◽  
East European ◽  
Albugo Candida ◽  
White Rust

AbstractWhite rust caused by oomycete pathogen Albugo candida is a significant disease of crucifer crops including Brassica juncea (mustard), a major oilseed crop of the Indian subcontinent. Earlier a resistance-conferring locus named AcB1-A5.1 was mapped in an east European gene pool line of B. juncea – Donskaja-IV. This line was tested along with some other lines of B. juncea (AABB), B. rapa (AA) and B. nigra (BB) for resistance to six isolates of A. candida collected from different mustard growing regions of India. Donskaja-IV was found to be completely resistant to all the tested isolates. Sequencing of a BAC spanning the locus AcB1-A5.1 showed the presence of a single CC-NB-LRR protein encoding R gene. The genomic sequence of the putative R gene with its native promoter and terminator was used for the genetic transformation of a susceptible Indian gene pool line Varuna and was found to confer complete resistance to all the isolates. This is the first white rust resistance-conferring gene described from Brassica species and has been named BjuWRR1. Allelic variants of the gene in B. juncea germplasm and orthologues in the Brassicaceae genomes were studied to understand the evolutionary dynamics of the BjuWRR1 gene.HighlightBjuWRR1, a CNL type R gene, was identified from an east European gene pool line of Brassica juncea and validated for conferring resistance to white rust by genetic transformation.

Expression and relationships of resistance to white rust (Albugo candida) at cotyledonary, seedling, and flowering stages in Brassica juncea germplasm from Australia, China, and India

2007 ◽  
Vol 58 (3) ◽  
pp. 259 ◽  
Author(s):  
C. X. Li ◽  
K. Sivasithamparam ◽  
G. Walton ◽  
P. Salisbury ◽  
W. Burton ◽  
...  
Keyword(s):  
Plant Growth ◽  
Brassica Juncea ◽  
Environmental Conditions ◽  
Host Resistance ◽  
Growth Stages ◽  
Sources Of Resistance ◽  
Albugo Candida ◽  
White Rust ◽  
China And India ◽  
Canola Quality

White rust (Albugo candida) is a highly destructive disease of oilseed Brassicas such as Brassica juncea and B. rapa. Most commercial B. juncea or B. rapa varieties are highly susceptible and yield losses from combined infection of leaves and inflorescences can be up to 20% or 60% in Australia and India, respectively. In Australia, canola-quality B. juncea has been developed to extend oilseed Brassica production into lower rainfall areas, with the first commercial B. juncea canola-quality variety planned for release in 2006. It is essential to identify useful sources of host resistance in B. juncea as breeding and/or selection of material for resistance is the most cost-effective method of delivering control for farmers. Three experiments were undertaken under controlled-environmental conditions to identify the best methods of characterising host resistance and to identify sources of resistance in B. juncea germplasm from Australia, China, and India. Forty-four B. juncea genotypes, viz. 22 from India, 12 from Australia, and 10 from China, were tested. Four Chinese genotypes (CBJ-001, CBJ-002, CBJ-003, CBJ-004) and one Australian genotype (JR049) consistently showed high resistance to A. candida across the different plant growth stages against a pathotype prevailing in Australia. Similarly, the most susceptible genotypes (viz. Indian genotypes RH781, RL1359, RH819) were extremely susceptible irrespective of the plant growth stage. Overall, although disease severity on cotyledons and leaves at the different growth stages was significantly and positively correlated, there was, however, no significant correlation between the number of stagheads and any of the other disease parameters measured. Our study demonstrates that controlled-environmental conditions are suitable for rapid identification of resistant genotypes and that genotypes with high levels of resistance can be reliably identified at the cotyledonary, seedling, or flowering stages.

Centennial Brown brown condiment mustard

2009 ◽  
Vol 89 (2) ◽  
pp. 337-340 ◽  
Author(s):  
G. Rakow ◽  
J. P. Raney ◽  
D. Rode ◽  
J. Relf-Eckstein
Keyword(s):  
Grain Yield ◽  
Brassica Juncea ◽  
Seed Quality ◽  
Leptosphaeria Maculans ◽  
Research Centre ◽  
Glucosinolate Content ◽  
Market Place ◽  
Improvement Program ◽  
Canadian Prairies ◽  
White Rust

Brown condiment mustard (Common Brown) has about 10% lower grain yield than oriental condiment mustard (yellow seeded), which both belong to the same species [Brassica juncea (L.) Czern.]. Yield improvements in brown condiment mustard are therefore of great importance. The Saskatoon Research Centre of AAFC initiated a condiment brown mustard improvement program in 1996 applying pedigree selection of single plants from the condiment brown mustard cultivar Blaze, which resulted in the selection and registration of the cultivar Centennial Brown. Centennial Brown yielded 3.2% more grain than the landrace Common Brown, on average over 81 location years in 9 yr of condiment mustard Co-op tests (1999–2007) and was well adapted to the mustard-growing areas of the Canadian prairies. Support for registration was based on 6 yr of Co-op tests. Centennial Brown had the same maturity (91 d) and was 5 cm taller (116 cm) than Common Brown. It had 1.5% lower fixed oil (36.6%) and 1.2% greater protein content (30.0%) compared with Common Brown. It had 0.4 g heavier seed (2.96 g 1000 seed-1) than Common Brown. Centennial Brown had 0.9 mg g seed-1 greater allyl glucosinolate content than Common Brown (9.15 mg g seed-1). Green seed counts were low in Centennial Brown (0.64%) compared with Common Brown (0.79%). This was confirmed in chlorophyll content measurements, 4.76 mg kg-1 for Centennial Brown and 5.24 mg kg-1 for Common Brown. Centennial Brown was resistant to blackleg disease [Leptosphaeria maculans (Desm.) Ces. et de Not.] and highly susceptible to the B. juncea races of white rust [Albugo candida (Pers.) Kuntze], equal to Common Brown. Centennial Brown will quickly replace Common Brown in the market place because of its increased grain yield and much superior seed quality. Key words: Brassica juncea (L.) Czern., cultivar description, grain yield, seed quality

INHERITANCE OF RESISTANCE TO Albugo Candida Race 2 IN MUSTARD (Brassica Juncea (L.) Czern.)

1988 ◽  
Vol 68 (2) ◽  
pp. 297-300 ◽  
Author(s):  
A. S. TIWARI ◽  
G. A. PETRIE ◽  
R. K. DOWNEY
Keyword(s):  
Brassica Juncea ◽  
Nuclear Genes ◽  
Growth Chamber ◽  
Inheritance Of Resistance ◽  
Resistant Parent ◽  
Albugo Candida ◽  
White Rust ◽  
Race 2 ◽  
Made In

The inheritance of resistance to white rust (Albugo Candida) race 2 in mustard (Brassica juncea) was studied in crosses involving one resistant and two susceptible cultivars. Inoculations were made in a growth chamber followed by growth of the plants under greenhouse conditions. The reaction of the F1 was like the resistant parent, indicating that resistance is dominant and controlled by nuclear genes. Backcrosses of F1 plants to the resistant parent showed the same reactions as that of the resistant parent. Backcrosses of F1 to the susceptible parents segregated in a 1:1 ratio of resistant to susceptible. The F2 segregation of resistant and susceptible plants gave a good fit to a 3:1 ratio. The study revealed that resistance is monogenic and could be easily transferred to adapted susceptible genotypes via backcrossing.Key words: Brassica juncea, Albugo Candida, mustard, white rust

AAC Brown 120 brown mustard

2018 ◽  
Vol 98 (6) ◽  
pp. 1406-1408
Author(s):  
Bifang Cheng ◽  
David J. Williams
Keyword(s):  
Brassica Juncea ◽  
Double Haploid ◽  
Microspore Culture ◽  
Western Canada ◽  
White Rust ◽  
Hybrid Plants ◽  
Check Variety ◽  
Oriental Mustard

AAC Brown 120 is a double-haploid (DH) brown mustard (Brassica juncea) variety. It was produced using a microspore culture from F1 hybrid plants resulting from a cross between the elite brown mustard DH line B474DH100 and the elite oriental mustard DH line O60DH151. AAC Brown 120 has significantly higher (12%) yield than the check variety Centennial Brown. It is resistant to white rust races 2a and 2v, whereas Centennial Brown is susceptible to races 2a and 2v. AAC Brown 120 is well adapted to all mustard-growing areas of western Canada.

scholarly journals BjuWRR1, a CC-NB-LRR gene identified in Brassica juncea, confers resistance to white rust caused by Albugo candida

2019 ◽  
Vol 132 (8) ◽  
pp. 2223-2236 ◽  
Author(s):  
Heena Arora ◽  
K. Lakshmi Padmaja ◽  
Kumar Paritosh ◽  
Nitika Mukhi ◽  
A. K. Tewari ◽  
...  
Keyword(s):  
Brassica Juncea ◽  
Albugo Candida ◽  
White Rust
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