scholarly journals Light Regulation of Carotenoid Biosynthesis in the Peel of Mandarin and Sweet Orange Fruits

2019 ◽  
Vol 10 ◽  
Author(s):  
Joanna Lado ◽  
Enriqueta Alós ◽  
Matías Manzi ◽  
Paul J.R. Cronje ◽  
Aurelio Gómez-Cadenas ◽  
...  
Keyword(s):  
Sweet Orange ◽  
Light Regulation ◽  
Carotenoid Biosynthesis

Carotenoid biosynthesis and quality characteristics of new hybrids between tangor (Citrus reticulata x C. sinensis) cv. ‘Murcott’ and sweet orange (C. sinensis) cv. ‘Pêra’

2019 ◽  
Vol 122 ◽  
pp. 461-470 ◽  
Author(s):  
Fabiane C. Petry ◽  
Fabio B. de Nadai ◽  
Mariângela Cristofani-Yaly ◽  
Rodrigo R. Latado ◽  
Adriana Z. Mercadante
Keyword(s):  
Sweet Orange ◽  
Carotenoid Biosynthesis ◽  
Quality Characteristics ◽  
Citrus Reticulata

Photoregulation of Carotenoid Biosynthesis in Mutants of Neurospora crassa: Activities of Enzymes Involved in the Synthesis and Conversion of Phytoene

1993 ◽  
Vol 48 (7-8) ◽  
pp. 570-574 ◽  
Author(s):  
Gerhard Sandmann
Keyword(s):  
Neurospora Crassa ◽  
Enzyme Level ◽  
Light Regulation ◽  
Carotenoid Biosynthesis ◽  
Phytoene Desaturase ◽  
Farnesyl Pyrophosphate ◽  
Farnesyl Pyrophosphate Synthase ◽  
Geranylgeranyl Pyrophosphate Synthase ◽  
Lycopene Cyclase ◽  
Carotenoid Mutants

Synthesis of carotenoids is photoregulated in many fungi including Neurospora crassa. In order to investigate the regulatory mechanism at the enzyme level, several carotenoid mutants of Neurospora were used to determine the activities of enzymes involved in the carotenoid bio synthetic pathway after growth under illumination or in darkness. Light stimulation of carotenoid formation was due to enhanced activities of three subsequent enzymes, geranylgeranyl pyrophosphate synthase, phytoene synthase, and phytoene desaturase indicating a coordinated regulation at the enzyme level. Farnesyl pyrophosphate synthase and lycopene cyclase were not involved in light regulation. Immunological studies showed that in the case of phytoene desaturase higher activity in the light originated from an increased amount of this enzyme in light-grown cultures.

Identification of novel members in sweet orange carotenoid biosynthesis gene families

2010 ◽  
Vol 6 (6) ◽  
pp. 905-914 ◽  
Author(s):  
Chunxian Chen ◽  
Marcio G. C. Costa ◽  
Qibin Yu ◽  
Gloria A. Moore ◽  
Fred G. Gmitter
Keyword(s):  
Sweet Orange ◽  
Carotenoid Biosynthesis ◽  
Gene Families ◽  
Biosynthesis Gene

scholarly journals Control of Development, Secondary Metabolism and Light-Dependent Carotenoid Biosynthesis by the Velvet Complex of Neurospora crassa

Genetics ◽  
2019 ◽  
Vol 212 (3) ◽  
pp. 691-710 ◽  
Author(s):  
Özlem Sarikaya Bayram ◽  
Anne Dettmann ◽  
Betim Karahoda ◽  
Nicola M. Moloney ◽  
Tereza Ormsby ◽  
...  
Keyword(s):  
Neurospora Crassa ◽  
Secondary Metabolism ◽  
Light Regulation ◽  
Carotenoid Biosynthesis ◽  
Nuclear Fraction ◽  
Iron Starvation ◽  
Life Styles ◽  
Velvet Complex ◽  
Developmental Features

Neurospora crassa is an established reference organism to investigate carotene biosynthesis and light regulation. However, there is little evidence of its capacity to produce secondary metabolites. Here, we report the role of the fungal-specific regulatory velvet complexes in development and secondary metabolism (SM) in N. crassa. Three velvet proteins VE-1, VE-2, VOS-1, and a putative methyltransferase LAE-1 show light-independent nucleocytoplasmic localization. Two distinct velvet complexes, a heterotrimeric VE-1/VE-2/LAE-1 and a heterodimeric VE-2/VOS-1 are found in vivo. The heterotrimer-complex, which positively regulates sexual development and represses asexual sporulation, suppresses siderophore coprogen production under iron starvation conditions. The VE-1/VE-2 heterodimer controls carotene production. VE-1 regulates the expression of >15% of the whole genome, comprising mainly regulatory and developmental features. We also studied intergenera functions of the velvet complex through complementation of Aspergillus nidulans veA, velB, laeA, vosA mutants with their N. crassa orthologs ve-1, ve-2, lae-1, and vos-1, respectively. Expression of VE-1 and VE-2 in A. nidulans successfully substitutes the developmental and SM functions of VeA and VelB by forming two functional chimeric velvet complexes in vivo, VelB/VE-1/LaeA and VE-2/VeA/LaeA, respectively. Reciprocally, expression of veA restores the phenotypes of the N. crassa ve-1 mutant. All N. crassa velvet proteins heterologously expressed in A. nidulans are localized to the nuclear fraction independent of light. These data highlight the conservation of the complex formation in N. crassa and A. nidulans. However, they also underline the intergenera similarities and differences of velvet roles according to different life styles, niches and ontogenetic processes.

An integrative analysis of transcriptome and proteome provides new insights into carotenoid biosynthesis and regulation in sweet orange fruits

2012 ◽  
Vol 75 (9) ◽  
pp. 2670-2684 ◽  
Author(s):  
Zhiyong Pan ◽  
Yunliu Zeng ◽  
Jianyong An ◽  
Junli Ye ◽  
Qiang Xu ◽  
...  
Keyword(s):  
Sweet Orange ◽  
Carotenoid Biosynthesis ◽  
Integrative Analysis
Sign in / Sign up

Export Citation Format

Share Document