Light regulation of carotenoid biosynthesis in the prasinophycean alga Mantoniella squamata

2002 ◽  
Vol 1 (8) ◽  
pp. 619-628 ◽  
Author(s):  
Karen Böhme ◽  
Christian Wilhelm ◽  
Reimund Goss
Keyword(s):  
Light Regulation ◽  
Carotenoid Biosynthesis ◽  
Mantoniella Squamata

Photoregulation of Carotenoid Biosynthesis in Mutants of Neurospora crassa: Activities of Enzymes Involved in the Synthesis and Conversion of Phytoene

1993 ◽  
Vol 48 (7-8) ◽  
pp. 570-574 ◽  
Author(s):  
Gerhard Sandmann
Keyword(s):  
Neurospora Crassa ◽  
Enzyme Level ◽  
Light Regulation ◽  
Carotenoid Biosynthesis ◽  
Phytoene Desaturase ◽  
Farnesyl Pyrophosphate ◽  
Farnesyl Pyrophosphate Synthase ◽  
Geranylgeranyl Pyrophosphate Synthase ◽  
Lycopene Cyclase ◽  
Carotenoid Mutants

Synthesis of carotenoids is photoregulated in many fungi including Neurospora crassa. In order to investigate the regulatory mechanism at the enzyme level, several carotenoid mutants of Neurospora were used to determine the activities of enzymes involved in the carotenoid bio synthetic pathway after growth under illumination or in darkness. Light stimulation of carotenoid formation was due to enhanced activities of three subsequent enzymes, geranylgeranyl pyrophosphate synthase, phytoene synthase, and phytoene desaturase indicating a coordinated regulation at the enzyme level. Farnesyl pyrophosphate synthase and lycopene cyclase were not involved in light regulation. Immunological studies showed that in the case of phytoene desaturase higher activity in the light originated from an increased amount of this enzyme in light-grown cultures.

scholarly journals Light Regulation of Carotenoid Biosynthesis in the Peel of Mandarin and Sweet Orange Fruits

2019 ◽  
Vol 10 ◽  
Author(s):  
Joanna Lado ◽  
Enriqueta Alós ◽  
Matías Manzi ◽  
Paul J.R. Cronje ◽  
Aurelio Gómez-Cadenas ◽  
...  
Keyword(s):  
Sweet Orange ◽  
Light Regulation ◽  
Carotenoid Biosynthesis

scholarly journals Control of Development, Secondary Metabolism and Light-Dependent Carotenoid Biosynthesis by the Velvet Complex of Neurospora crassa

Genetics ◽  
2019 ◽  
Vol 212 (3) ◽  
pp. 691-710 ◽  
Author(s):  
Özlem Sarikaya Bayram ◽  
Anne Dettmann ◽  
Betim Karahoda ◽  
Nicola M. Moloney ◽  
Tereza Ormsby ◽  
...  
Keyword(s):  
Neurospora Crassa ◽  
Secondary Metabolism ◽  
Light Regulation ◽  
Carotenoid Biosynthesis ◽  
Nuclear Fraction ◽  
Iron Starvation ◽  
Life Styles ◽  
Velvet Complex ◽  
Developmental Features

Neurospora crassa is an established reference organism to investigate carotene biosynthesis and light regulation. However, there is little evidence of its capacity to produce secondary metabolites. Here, we report the role of the fungal-specific regulatory velvet complexes in development and secondary metabolism (SM) in N. crassa. Three velvet proteins VE-1, VE-2, VOS-1, and a putative methyltransferase LAE-1 show light-independent nucleocytoplasmic localization. Two distinct velvet complexes, a heterotrimeric VE-1/VE-2/LAE-1 and a heterodimeric VE-2/VOS-1 are found in vivo. The heterotrimer-complex, which positively regulates sexual development and represses asexual sporulation, suppresses siderophore coprogen production under iron starvation conditions. The VE-1/VE-2 heterodimer controls carotene production. VE-1 regulates the expression of >15% of the whole genome, comprising mainly regulatory and developmental features. We also studied intergenera functions of the velvet complex through complementation of Aspergillus nidulans veA, velB, laeA, vosA mutants with their N. crassa orthologs ve-1, ve-2, lae-1, and vos-1, respectively. Expression of VE-1 and VE-2 in A. nidulans successfully substitutes the developmental and SM functions of VeA and VelB by forming two functional chimeric velvet complexes in vivo, VelB/VE-1/LaeA and VE-2/VeA/LaeA, respectively. Reciprocally, expression of veA restores the phenotypes of the N. crassa ve-1 mutant. All N. crassa velvet proteins heterologously expressed in A. nidulans are localized to the nuclear fraction independent of light. These data highlight the conservation of the complex formation in N. crassa and A. nidulans. However, they also underline the intergenera similarities and differences of velvet roles according to different life styles, niches and ontogenetic processes.

scholarly journals Comparative transcriptomic and metabolomic analyses of carotenoid biosynthesis reveal the basis of white petal color in Brassica napus

Planta ◽  
2021 ◽  
Vol 253 (1) ◽  
Author(s):  
Ledong Jia ◽  
Junsheng Wang ◽  
Rui Wang ◽  
Mouzheng Duan ◽  
Cailin Qiao ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Molecular Mechanisms ◽  
Flower Color ◽  
Carotenoid Biosynthesis ◽  
Differentially Expressed ◽  
Transcriptome Data ◽  
Oilseed Crops ◽  
Qrt Pcr ◽  
Carotenoid Metabolism ◽  
Rapeseed Cultivar

Abstract Main conclusion The molecular mechanism underlying white petal color in Brassica napus was revealed by transcriptomic and metabolomic analyses. Abstract Rapeseed (Brassica napus L.) is one of the most important oilseed crops worldwide, but the mechanisms underlying flower color in this crop are known less. Here, we performed metabolomic and transcriptomic analyses of the yellow-flowered rapeseed cultivar ‘Zhongshuang 11’ (ZS11) and the white-flowered inbred line ‘White Petal’ (WP). The total carotenoid contents were 1.778-fold and 1.969-fold higher in ZS11 vs. WP petals at stages S2 and S4, respectively. Our findings suggest that white petal color in WP flowers is primarily due to decreased lutein and zeaxanthin contents. Transcriptome analysis revealed 10,116 differentially expressed genes with a fourfold or greater change in expression (P-value less than 0.001) in WP vs. ZS11 petals, including 1,209 genes that were differentially expressed at four different stages and 20 genes in the carotenoid metabolism pathway. BnNCED4b, encoding a protein involved in carotenoid degradation, was expressed at abnormally high levels in WP petals, suggesting it might play a key role in white petal formation. The results of qRT-PCR were consistent with the transcriptome data. The results of this study provide important insights into the molecular mechanisms of the carotenoid metabolic pathway in rapeseed petals, and the candidate genes identified in this study provide a resource for the creation of new B. napus germplasms with different petal colors.

scholarly journals Carotenoid Biosynthesis in Photosynthetic Bacteria

1989 ◽  
Vol 264 (22) ◽  
pp. 13109-13113
Author(s):  
G E Bartley ◽  
P A Scolnik
Keyword(s):  
Photosynthetic Bacteria ◽  
Carotenoid Biosynthesis

scholarly journals Controlled Transcription of Regulator Gene carS by Tet-on or by a Strong Promoter Confirms Its Role as a Repressor of Carotenoid Biosynthesis in Fusarium fujikuroi

2020 ◽  
Vol 9 (1) ◽  
pp. 71
Author(s):  
Julia Marente ◽  
Javier Avalos ◽  
M. Carmen Limón
Keyword(s):  
Wild Strain ◽  
Ring Finger ◽  
Carotenoid Biosynthesis ◽  
Negative Regulator ◽  
Fusarium Fujikuroi ◽  
Structural Genes ◽  
Gene Encoding ◽  
In The Wild ◽  
Set Up

Carotenoid biosynthesis is a frequent trait in fungi. In the ascomycete Fusarium fujikuroi, the synthesis of the carboxylic xanthophyll neurosporaxanthin (NX) is stimulated by light. However, the mutants of the carS gene, encoding a protein of the RING finger family, accumulate large NX amounts regardless of illumination, indicating the role of CarS as a negative regulator. To confirm CarS function, we used the Tet-on system to control carS expression in this fungus. The system was first set up with a reporter mluc gene, which showed a positive correlation between the inducer doxycycline and luminescence. Once the system was improved, the carS gene was expressed using Tet-on in the wild strain and in a carS mutant. In both cases, increased carS transcription provoked a downregulation of the structural genes of the pathway and albino phenotypes even under light. Similarly, when the carS gene was constitutively overexpressed under the control of a gpdA promoter, total downregulation of the NX pathway was observed. The results confirmed the role of CarS as a repressor of carotenogenesis in F. fujikuroi and revealed that its expression must be regulated in the wild strain to allow appropriate NX biosynthesis in response to illumination.

A phosphor converted RED light for automotive LED rear lamp and compliance with ECE light regulation

Optik ◽  
2021 ◽  
Vol 234 ◽  
pp. 166590
Author(s):  
Seok-Ho Jeong ◽  
Jung-Young Kim ◽  
Xiao Xiao ◽  
Young-Suk Kim
Keyword(s):  
Red Light ◽  
Light Regulation ◽  
Rear Lamp
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