scholarly journals Identification of a Novel Partitivirus of Trichoderma harzianum NFCF319 and Evidence for the Related Antifungal Activity

2018 ◽  
Vol 9 ◽  
Author(s):  
Jeesun Chun ◽  
Han-Eul Yang ◽  
Dae-Hyuk Kim
Keyword(s):  
Antifungal Activity ◽  
Trichoderma Harzianum

Antifungal activity of Trichoderma harzianum and T. koningiopsis against Fusarium solani in seed germination and vigor of Miahuateco chili seedlings

2021 ◽  
Vol 39 (2) ◽  
Author(s):  
Leydi Miguel-Ferrer ◽  
Omar Romero-Arenas ◽  
Petra Andrade-Hoyos ◽  
Primo Sánchez-Morales ◽  
José Antonio Rivera-Tapia ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Seed Germination ◽  
Trichoderma Harzianum ◽  
Fusarium Solani ◽  
Para Determinar ◽  
Santa Maria

El chile es la segunda hortaliza de mayor producción en México. El objetivo de la investigación fue evaluar la actividad antagónica <em>in vitro</em> e <em>in vivo</em> de <em>Trichoderma harzianum</em> (T-H4) y <em>T. koningiopsis</em> (T-K11) <em>versus Fusarium solani</em> (MX-MIC 798) en la germinación y establecimiento de plántula de chile Miahuateco. Se utilizó la técnica de cultivo dual para determinar el porcentaje de inhibición de crecimiento radial (PICR) de la cepa MX-MIC 798. Además, se analizó el porcentaje de germinación en semillas de chile Miahuateco en vivero, así como la mortalidad de plántulas y grado de severidad a los 40 días después del trasplante (ddt) en Santa María Tecomavaca, Oaxaca, a través de biocontrol y control químico (Mancozeb 80®). La cepa T-H4 presentó el nivel antagónico PICR más alto (53.3%) <em>in vitro</em> y clase II en la escala de Bell, asimismo obtuvo 82% de germinación en semillas de chile Miahuateco en vivero y 48% de mortalidad en campo; de manera que igualó al control químico y superó a <em>T. koningiopsis</em> T-K11. La actividad antifúngica de <em>Trichoderma</em> spp., ofrecen una alternativa para el biocontrol de la marchitez y necrosis en raíz del cultivo de chile Miahuateco causada por <em>F. solani</em> MX-MIC 798.

scholarly journals Antifungal activity of several isolates of Trichoderma against Cladosporium and Botrytis

2018 ◽  
Vol 8 (1) ◽  
pp. 88-91 ◽  
Author(s):  
M. Skaptsov ◽  
S. Smirnov ◽  
M. Kutsev ◽  
O. Uvarova ◽  
T. Sinitsyna ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Trichoderma Harzianum ◽  
Its Region ◽  
Botanical Garden ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Soil Samples ◽  
Morphological Observation ◽  
The South

<p><em>Trichoderma</em> isolates (SSBGT07, SSBGT08, SSBGT09, SSBGT10) were isolated from the soil samples of the South-Siberian Botanical Garden and identified using morphological observation and ITS region analysis as <em>Trichoderma harzianum</em>, <em>T. asperellum, T. ghanense</em>, and <em>T. longibranchiatum</em>. Antagonistic activity against <em>Cladosporium </em>sp. and<em> Botrytis </em>sp. was evaluated <em>in vitro</em>. All isolates showed antagonistic effect by competition against <em>Cladosporium </em>sp. <em>T. asperellum </em>and <em>T. longibranchiatum</em> showed antagonism against <em>Botrytis </em>sp. All isolates showed hyper sporulation on the sclerotia of <em>Botrytis</em> sp. (except the <em>T. ghanense</em>) and colonies of the <em>Cladosporium</em> sp. Our study provides new isolates that affect the <em>Cladosporium </em>sp. and<em> Botrytis </em>sp.</p>

scholarly journals Dithiosulfinates and Sulfoxides with Antifungal Activity from Bulbs of Allium sativum L. var. Voghiera

2018 ◽  
Vol 13 (9) ◽  
pp. 1934578X1801300
Author(s):  
Bruna de Falco ◽  
Giuliano Bonanomi ◽  
Virginia Lanzotti
Keyword(s):  
Antimicrobial Activity ◽  
Nmr Spectroscopy ◽  
Antifungal Activity ◽  
Botrytis Cinerea ◽  
Trichoderma Harzianum ◽  
Structure Elucidation ◽  
Allium Sativum ◽  
Fungal Species ◽  
Phytochemical Analysis ◽  
Spectroscopic Analyses

A bioassay guided phytochemical analysis of the bulbs of Allium sativum L. var. Voghiera, typical of Voghiera, Ferrara (Italy), allowed the isolation of six new sulfur compounds with dithiosulfinates and sulfoxides functionalities. Structure elucidation of the isolated compounds was carried out by spectroscopic analyses, including NMR spectroscopy and MS spectrometry. Compounds showed significant antimicrobial activity towards two fungal species, the air-borne pathogen Botrytis cinerea and the beneficial fungus Trichoderma harzianum.

Partial silencing of a hydroxy-methylglutaryl-CoA reductase-encoding gene in Trichoderma harzianum CECT 2413 results in a lower level of resistance to lovastatin and lower antifungal activity

2007 ◽  
Vol 44 (4) ◽  
pp. 269-283 ◽  
Author(s):  
Rosa Elena Cardoza ◽  
María Rosa Hermosa ◽  
Juan Antonio Vizcaíno ◽  
Fran González ◽  
Antonio Llobell ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Trichoderma Harzianum ◽  
Encoding Gene ◽  
Coa Reductase

scholarly journals Disruption of the ech42 (Endochitinase-Encoding) Gene Affects Biocontrol Activity in Trichoderma harzianum P1

1999 ◽  
Vol 12 (5) ◽  
pp. 419-429 ◽  
Author(s):  
S. L. Woo ◽  
B. Donzelli ◽  
F. Scala ◽  
R. Mach ◽  
G. E. Harman ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Trichoderma Harzianum ◽  
Pythium Ultimum ◽  
Single Copy ◽  
Wild Type ◽  
Gene Encoding ◽  
Culture Filtrates ◽  
In Vitro Antifungal Activity ◽  
Better Than

The biocontrol strain P1 of Trichoderma harzianum was genetically modified by targeted disruption of the single-copy ech42 gene encoding for the secreted 42-kDa endochitinase (CHIT42). Stable mutants in which ech42 was interrupted, and unable to produce CHIT42, were obtained and characterized. These mutants lacked the ech42 transcript, the protein, and endochitinase activity in culture filtrates, and they were unable to clear a medium containing colloidal chitin. Other chitinolytic and glucanolytic enzymes expressed during mycoparasitism were not affected by the disruption of ech42. The disrupted mutant D11 grew and sporulated similarly to the wild type. In vitro antifungal activity of the ech42 disruptant culture filtrates against Botrytis cinerea and Rhizoctonia solani was reduced about 40%, compared with wild type; antifungal activity was fully restored by adding an equivalent amount of CHIT42 as secreted by P1. The mutant exhibited the same biocontrol effect against Pythium ultimum as strain P1, but the antagonism against B. cinerea on bean leaves by the mutant was significantly reduced (33% less biocontrol), compared with strain P1. Conversely, the endochitinase-deficient mutant performed better than the wild type (16% improvement of survival) in biocontrol experiments in soil infested with the soilborne fungus R. solani. These results indicate that the antagonistic interaction between the T. harzianum strain and various fungal hosts is based on different mechanisms.

scholarly journals Increased Antifungal Activity of Trichoderma harzianum Transformants That Overexpress a 33-kDa Chitinase

1999 ◽  
Vol 89 (3) ◽  
pp. 254-261 ◽  
Author(s):  
M. Carmen Limón ◽  
José A. Pintor-Toro ◽  
Tahía Benítez
Keyword(s):  
Antifungal Activity ◽  
Trichoderma Harzianum ◽  
Southern Blotting ◽  
Biocontrol Agent ◽  
Chitinase Activity ◽  
Restriction Pattern ◽  
Constitutive Promoter ◽  
Wild Type ◽  
Amds Gene ◽  
Culture Supernatants

Transformants of the biocontrol agent Trichoderma harzianum strain CECT 2413 that overexpressed a 33-kDa chitinase (Chit33) were obtained and characterized. Strain CECT 2413 was cotransformed with the amdS gene and its own chit33 gene under the control of the pki constitutive promoter from T. reesei. Southern blotting indicated that the chit33 gene was integrated ectopically, mostly in tandem. Some transformants showed the same restriction pattern, indicating preferable sites of integration. There was no correlation between the number of integrated copies and the level of expression of the chit33 gene in the transformants. When grown in glucose, the extracellular chitinase activity of the transformants was up to 200-fold greater than that of the wild type, whereas in chitin, the activity of both the transformants and the wild type was similar. Under both conditions, the transformants were more effective in inhibiting the growth of Rhizoctonia solani as compared with the wild type. Similar results were obtained when culture supernatants from the transformants and the wild type were tested against R. solani.

pH and Pac1 control development and antifungal activity in Trichoderma harzianum

2007 ◽  
Vol 44 (12) ◽  
pp. 1355-1367 ◽  
Author(s):  
Miguel A. Moreno-Mateos ◽  
Jesús Delgado-Jarana ◽  
Antonio C. Codón ◽  
Tahía Benítez
Keyword(s):  
Antifungal Activity ◽  
Trichoderma Harzianum

scholarly journals Cytotoxicity and Antifungal Activities of Root Bark of Calotropis gigantea

2010 ◽  
Vol 2 (2) ◽  
pp. 38-41 ◽  
Author(s):  
M Rowshanul Habib ◽  
M Ashraful Alam ◽  
MA Haque ◽  
Farjana Nikkon ◽  
M Rezaul Karim
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Trichoderma Harzianum ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Root Bark ◽  
Calotropis Gigantea ◽  
Penicillium Sp

In this study, methanol extract from the root bark of Calotropis gigantea L. and its petroleum ether (40°C-60°C), chloroform and ethyl acetate soluble fractions were tested for their cytotoxic activity against brine shrimp nauplii (Artemia salina, Leach) and for antifungal activity against Aspergillus flavus, Aspergillus niger, Penicillium sp and Trichoderma harzianum. Thin layer chromatography (TLC) screening showed that methanol extract and its different fractions contained different type compounds such as steroid, terpene, glycoside, heterocyclic and flavonoid. In brine shrimp lethality bioassay, it was found that chloroform fraction was highly cytotoxic (LD50 14.72 μg/ml) among the tested samples. Though methanol extract and ethyl acetate fraction have no activity against all the tested fungi but petroleum ether and chloroform fractions showed potent activity against Aspergillus niger, Penicillium sp, Trichoderma harzianum and Aspergillus niger, Trichoderma harzianum, respectively, in antifungal activity test.  Key words: Calotropis gigantea; Methanol extract; Antifungal activity; Cytotoxicty.DOI: 10.3329/sjps.v2i2.2187Stamford Journal of Pharmaceutical Sciences Vol.2(2) 2009: 38-41

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