scholarly journals A Phospholipase C-Like Protein From Ricinus communis Increases Hydroxy Fatty Acids Accumulation in Transgenic Seeds of Camelina sativa

2018 ◽  
Vol 9 ◽  
Author(s):  
Niranjan Aryal ◽  
Chaofu Lu
Keyword(s):  
Fatty Acids ◽  
Phospholipase C ◽  
Ricinus Communis ◽  
Camelina Sativa ◽  
Hydroxy Fatty Acids ◽  
Transgenic Seeds

scholarly journals Extracellular lipids of Camelina sativa: Characterization of cutin and suberin reveals typical polyester monomers and novel functionalized dicarboxylic fatty acids

2020 ◽  
Author(s):  
Fakhria M. Razeq ◽  
Dylan K. Kosma ◽  
Débora França ◽  
Owen Rowland ◽  
Isabel Molina
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Caffeic Acid ◽  
Seed Coat ◽  
Abiotic Stresses ◽  
Camelina Sativa ◽  
Hydroxy Fatty Acids ◽  
Oxidation Products ◽  
List Type ◽  
Biotic And Abiotic Stresses

AbstractCamelina sativa is relatively drought tolerant and requires less fertilizer than other oilseed crops. Various lipid- and phenolic-based extracellular barriers of plants help to protect them against biotic and abiotic stresses. These barriers, which consist of solvent-insoluble polymeric frameworks and solvent-extractable waxes, include the cuticle of aerial plant surfaces and suberized cell walls found, for example, in periderms and seed coat. Cutin, the polymeric matrix of the cuticle, and the aliphatic domain of suberin are fatty acid- and glycerol-based polyesters. These polyesters were investigated by base-catalyzed transesterification of C. sativa aerial and underground delipidated tissues followed by gas chromatographic analysis of the released monomer mixtures. Seed coat and root suberin had similar compositions, with 18-hydroxyoctadecenoic and 1,18-octadecenedioic fatty acids being the dominant species. Root suberin presented a typical lamellar ultrastructure, but seed coats showed almost imperceptible, faint dark bands. Leaf and stem lipid polyesters were composed of fatty acids (FA), dicarboxylic acids (DCA), ω-hydroxy fatty acids (OHFA) and hydroxycinnamic acid derivatives (HCA). Dihydroxypalmitate (DHP) and caffeic acid were the major constituents of leaf cutin, whereas stem cutin presented similar molar proportions in several monomers across the four classes. Unlike the leaf cuticle, the C. sativa stem cuticle presented lamellar structure by transmission electron microscopy. Flower cutin was dominated by DHP and did not contain aromatics. We found striking differences between the lipid polyester monomer compositions of aerial tissues of C. sativa and that of its close relatives Arabidopsis thaliana and Brassica napus.Graphical AbstractCamelina sativa leaf and stem cutin is dominated by 16:0 dihydroxy fatty acid and aromatics, with dicarboxylic fatty acids representing 20-30 % of the monomers. Suberin of root and seed coat is largely composed of 18:1 dicarboxylic and ω-hydroxy fatty acids.Highlight bullet pointsCamelina sativa is an oil crop tolerant to biotic and abiotic stressesExtracellular lipid polyesters may in part confer these attributesDihydroxypalmitate and caffeic acid were major components of C. sativa leaf cutinFlower cutin lacked aromatics and contained monomers not previously reportedRoot and seed coat suberin was dominated by 18:1 ω-hydroxy and dicarboxylic fatty acidsC18 monounsaturated photo-oxidation products were found in leaf cutin and suberin

The involvement of phospholipid:diacylglycerol acyltransferases in triacylglycerol production

2000 ◽  
Vol 28 (6) ◽  
pp. 703-705 ◽  
Author(s):  
A. Banaś ◽  
A. Dahlqvist ◽  
U. Ståhl ◽  
M. Lenman ◽  
S. Stymne
Keyword(s):  
Fatty Acids ◽  
Arabidopsis Thaliana ◽  
Ricinoleic Acid ◽  
Ricinus Communis ◽  
Vernolic Acid ◽  
Hydroxy Fatty Acids ◽  
Oil Seeds ◽  
Medium Chain ◽  
Vegetative Tissues ◽  
Unusual Fatty Acids

We have characterized three CoA-independent types of enzyme, phospholipases, phospholipid: diacylglycerol acyltransferases (PDATs) and cholinephosphotransferases, responsible for the removal of unusual fatty acids from phosphatidylcholine (PC) in microsomal preparations from developing oil seeds. The metabolism of sn-2-[14C]acyl-PC was monitored in microsomal preparations from various oilseeds having either medium-chain, acetylenic, epoxy or hydroxy fatty acids as their major fatty acids in the oil. The results indicate that PDAT plays a major role in removing ricinoleic acid and vernolic acid from phospholipids in Ricinus communis and Crepis palaestina seeds, respectively. However, vernolic, crepenynic and capric acids are primarily removed from phospholipids by phospholipases in Euphorbia lagascae, Crepis rubra and elm seeds, respectively. Further, we show that significant PDAT activity is also present in vegetative tissues of Arabidopsis thaliana.

scholarly journals Occurrence of 2-hydroxy fatty acids in animal tissues

1963 ◽  
Vol 4 (2) ◽  
pp. 139-143 ◽  
Author(s):  
Yasuo Kishimoto ◽  
Norman S. Radin
Keyword(s):  
Fatty Acids ◽  
Hydroxy Fatty Acids ◽  
Animal Tissues

scholarly journals Optimization and Engineering of a Self-Sufficient CYP102 Enzyme from Bacillus amyloliquefaciens towards Synthesis of In-Chain Hydroxy Fatty Acids

Catalysts ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 665
Author(s):  
Li Zong ◽  
Yan Zhang ◽  
Zhengkang Shao ◽  
Yingwu Wang ◽  
Zheng Guo ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Palmitic Acid ◽  
Bacillus Amyloliquefaciens ◽  
Catalytic Domain ◽  
Hydroxy Fatty Acids ◽  
Saturation Mutagenesis ◽  
Long Chain Fatty Acids ◽  
Medical Field ◽  
Biotransformation Process ◽  
Total Product

Cytochrome P450 (CYP) mediated enzymatic hydroxylation of fatty acids present a green alternative to chemical synthesis of hydroxy fatty acids (HFAs), which are high-value oleochemicals with various uses in materials industry and medical field. Although many CYPs require the presence of additional reductase proteins for catalytic activity, self-sufficient CYPs have their reductase partner naturally fused into their catalytic domain, leading to a greatly simplified biotransformation process. A recently discovered self-sufficient CYP, BAMF2522 from Bacillus amyloliquefaciens DSM 7, exhibits novel regioselectivity by hydroxylating in-chain positions of palmitic acid generating ω-1 to ω-7 HFAs, a rare regiodiversity profile among CYPs. Besides, F89I mutant of BAMF2522 expanded hydroxylation up to ω-9 position of palmitic acid. Here, we further characterize this enzyme by determining optimum temperature and pH as well as thermal stability. Moreover, using extensive site-directed and site-saturation mutagenesis, we obtained BAMF2522 variants that demonstrate greatly increased regioselectivity for in-chain positions (ω-4 to ω-9) of various medium to long chain fatty acids. Remarkably, when a six-residue mutant was reacted with palmitic acid, 84% of total product content was the sum of ω-7, ω-8 and ω-9 HFA products, the highest in-chain selectivity observed to date with a self-sufficient CYP. In short, our study demonstrates the potential of a recently identified CYP and its mutants for green and sustainable production of a variety of in-chain hydroxy enriched HFAs.

Sign in / Sign up

Export Citation Format

Share Document