scholarly journals Ethylene-Induced Hydrogen Sulfide Negatively Regulates Ethylene Biosynthesis by Persulfidation of ACO in Tomato Under Osmotic Stress

2018 ◽  
Vol 9 ◽  
Author(s):  
Honglei Jia ◽  
Sisi Chen ◽  
Dan Liu ◽  
Johannes Liesche ◽  
Cong Shi ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Osmotic Stress ◽  
Ethylene Biosynthesis

scholarly journals Hydrogen sulfide and potassium synergistically induce osmotic stress tolerance through regulation of H+-ATPase activity and sugar metabolism in tomato seedlings

2021 ◽  
Author(s):  
Manzer H. Siddiqui ◽  
Soumya Mukherjee ◽  
Saud Alamri ◽  
Yanbo Hu ◽  
Abdullah Alamri ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Osmotic Stress ◽  
Atpase Activity ◽  
Channel Blocker ◽  
Sugar Metabolism ◽  
Decisive Role ◽  
K Channel ◽  
Tomato Seedlings ◽  
Osmotic Stress Tolerance

Abstract Potassium (K) is an essential macronutrient which is known to regulate key metabolic processes, modulate enzyme activity and plays a decisive role in osmotic adjustment in plants. Present work evaluates the role of K in the regulation of endogenous hydrogen sulfide (H2S) signaling in modulating the tolerance of tomato (Solanum lycopersicum L. Mill.) seedlings to osmotic stress. The findings reveal that exposure of seedlings to 15% (w/v) polyethylene glycol 8000 (PEG) led to a substantial decrease in leaf K content which was associated with reduced H+-ATPase activity. Exogenous application of K to the stressed seedlings significantly improved endogenous K content. Treatment with sodium orthovanadate (SOV, PM H+-ATPase inhibitor) and tetraethylammonium chloride (TEA, K channel blocker) suggests that exogenous K stimulated H+-ATPase activity that further regulated endogenous K content in tomato seedlings subjected to osmotic stress. Moreover, reduction in H+-ATPase activity by hypotaurine (H2S scavenger) substantiates the role of endogenous H2S in the regulation of H+-ATPase activity. Elevation in endogenous K content enhanced the biosynthesis of hydrogen sulfide (H2S) through enhancing the synthesis of cysteine, the H2S precursor. Synergistic action of H2S and K effectively neutralized osmotic stress by regulating sugar metabolism that resulted in osmotic adjustment, as witnessed by reduced water loss and improved hydration level of the stressed seedlings. Cross talk of H2S and K also assisted the seedlings in the activation of antioxidant enzymes that controlled the generation of reactive oxygen species and led to the protection against oxidative stress. The integrative role of H2S and K signaling was validated using hypotaurine (H2S scavenger) and TEA (K channel blocker) which weakened the protection against osmotic stress induced impairments. In conclusion, exogenous K and endogenous H2S regulate H+-ATPase activity which plays a decisive role in the maintenance of endogenous K homeostasis. Thus, present work reveals that K and H2S crosstalk is essential for modulation of osmotic stress tolerance in tomato seedlings.

Hydrogen sulfide counteracts chlorophyll loss in sweetpotato seedling leaves and alleviates oxidative damage against osmotic stress

2009 ◽  
Vol 58 (3) ◽  
pp. 243-250 ◽  
Author(s):  
Hua Zhang ◽  
Yong-Kang Ye ◽  
Song-Hua Wang ◽  
Jian-Ping Luo ◽  
Jun Tang ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Osmotic Stress ◽  
Oxidative Damage

scholarly journals Delaying Broccoli Floret Yellowing by Phytosulfokine α Application During Cold Storage

2021 ◽  
Vol 8 ◽  
Author(s):  
Morteza Soleimani Aghdam ◽  
Majid Alikhani-Koupaei ◽  
Raheleh Khademian
Keyword(s):  
Gene Expression ◽  
Hydrogen Sulfide ◽  
Nutritional Value ◽  
Acc Oxidase ◽  
Acc Synthase ◽  
Ethylene Biosynthesis ◽  
Pheophorbide A ◽  
Postharvest Life ◽  
Cysteine Desulfhydrase ◽  
Pheophorbide A Oxygenase

During postharvest life, broccoli suffers from floret yellowing confining its economic and nutritional value. The objective of the present study was to explore the mechanisms employed by phytosulfokine α (PSKα) at 150 nM for delaying floret yellowing in broccoli during storage at 4°C for 28 days. Our results showed that the higher endogenous accumulation of hydrogen sulfide (H2S) resulting from the higher gene expression and activities of l-cysteine desulfhydrase (LCD) and d-cysteine desulfhydrase (DCD) in broccoli floret treated with 150 nM PSKα may serve as an endogenous signaling molecule for delaying senescence. Moreover, the suppressed ethylene biosynthesis in broccoli floret treated with 150 nM PSKα might be ascribed to lower gene expression and activities of ACC synthase (ACS) and ACC oxidase (ACO). Furthermore, lower gene expression and activities of Mg2+ dechelatase (MDC), pheophytinase (PPH), and pheophorbide a oxygenase (PaO) might be the reasons for the higher accumulation of chlorophyll in broccoli floret treated with 150 nM PSKα. Based on our findings, exogenous PSKα application could be employed as signaling bioactive hormone for retarding floret yellowing of broccoli during storage at 4°C for 28 days.

scholarly journals HYDROGEN SULFIDE DONOR INFLUENCE ON STATE OF ANTIOXIDANT SYSTEM OF WHEAT SEEDLINGS UNDER OSMOTIC STRESS

2018 ◽  
Vol 50 (1) ◽  
pp. 29-38 ◽  
Author(s):  
Yu.E. Kolupaev ◽  
◽  
K.M. Firsova ◽  
M.V. Shvidenko ◽  
T.O. Yastreb ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Osmotic Stress ◽  
Antioxidant System ◽  
Wheat Seedlings ◽  
Donor Influence

Hydrogen sulfide mediates DNA methylation to enhance osmotic stress tolerance in Setaria italica L.

2020 ◽  
Vol 453 (1-2) ◽  
pp. 355-370
Author(s):  
Xuefeng Hao ◽  
Zhuping Jin ◽  
Zhiqing Wang ◽  
Wensheng Qin ◽  
Yanxi Pei
Keyword(s):  
Dna Methylation ◽  
Hydrogen Sulfide ◽  
Osmotic Stress ◽  
Stress Tolerance ◽  
Setaria Italica ◽  
Osmotic Stress Tolerance

scholarly journals Hydrogen Sulfide Inhibits Fruit Softening by Regulating Ethylene Synthesis and Signaling Pathway in Tomato (Solanum lycopersicum)

HortScience ◽  
2019 ◽  
Vol 54 (10) ◽  
pp. 1824-1830 ◽  
Author(s):  
Kang-Di Hu ◽  
Xiao-Yue Zhang ◽  
Sha-Sha Wang ◽  
Jun Tang ◽  
Feng Yang ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Signaling Pathway ◽  
Tomato Fruit ◽  
Ethylene Biosynthesis ◽  
Fruit Softening ◽  
Fruit Firmness ◽  
Cell Wall Degrading Enzymes ◽  
Postharvest Storage ◽  
Transcript Accumulation ◽  
Gene Expressions

Hydrogen sulfide (H2S) has been proven to be a multifunctional signaling molecule in plants. In this study, we attempted to explore the effects of H2S on the climacteric fruit tomato during postharvest storage. H2S fumigation for 1 d was found to delay the peel color transition from green to red and decreased fruit firmness induced by ethylene. Further investigation showed that H2S fumigation downregulated the activities and gene expressions of cell wall–degrading enzymes pectin lyase (PL), polygalacturonase (PG), and cellulase. Furthermore, H2S fumigation downregulated the expression of ethylene biosynthesis genes SlACS2 and SlACS3. Ethylene treatment for 1 d was found to induce the expression of SlACO1, SlACO3, and SlACO4 genes, whereas the increase was significantly inhibited by H2S combined with ethylene. Furthermore, H2S decreased the transcript accumulation of ethylene receptor genes SlETR5 and SlETR6 and ethylene transcription factors SlCRF2 and SlERF2. The correlation analysis suggested that the fruit firmness was negatively correlated with ethylene biosynthesis and signaling pathway. The current study showed that exogenous H2S could inhibit the synthesis of endogenous ethylene and regulate ethylene signal transduction, thereby delaying fruit softening and the ripening process of tomato fruit during postharvest storage.

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