scholarly journals Hydrogen sulfide is involved in the regulation of ascorbate-glutathione cycle by exogenous ABA in wheat seedling leaves under osmotic stress

2017 ◽  
Vol 45 (3) ◽  
pp. 411-420 ◽  
Author(s):  
C. Shan ◽  
S. Zhang ◽  
Y. Zhou
Keyword(s):  
Hydrogen Sulfide ◽  
Osmotic Stress ◽  
Wheat Seedling ◽  
Exogenous Aba ◽  
Glutathione Cycle

scholarly journals Hydrogen sulfide and potassium synergistically induce osmotic stress tolerance through regulation of H+-ATPase activity and sugar metabolism in tomato seedlings

2021 ◽  
Author(s):  
Manzer H. Siddiqui ◽  
Soumya Mukherjee ◽  
Saud Alamri ◽  
Yanbo Hu ◽  
Abdullah Alamri ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Osmotic Stress ◽  
Atpase Activity ◽  
Channel Blocker ◽  
Sugar Metabolism ◽  
Decisive Role ◽  
K Channel ◽  
Tomato Seedlings ◽  
Osmotic Stress Tolerance

Abstract Potassium (K) is an essential macronutrient which is known to regulate key metabolic processes, modulate enzyme activity and plays a decisive role in osmotic adjustment in plants. Present work evaluates the role of K in the regulation of endogenous hydrogen sulfide (H2S) signaling in modulating the tolerance of tomato (Solanum lycopersicum L. Mill.) seedlings to osmotic stress. The findings reveal that exposure of seedlings to 15% (w/v) polyethylene glycol 8000 (PEG) led to a substantial decrease in leaf K content which was associated with reduced H+-ATPase activity. Exogenous application of K to the stressed seedlings significantly improved endogenous K content. Treatment with sodium orthovanadate (SOV, PM H+-ATPase inhibitor) and tetraethylammonium chloride (TEA, K channel blocker) suggests that exogenous K stimulated H+-ATPase activity that further regulated endogenous K content in tomato seedlings subjected to osmotic stress. Moreover, reduction in H+-ATPase activity by hypotaurine (H2S scavenger) substantiates the role of endogenous H2S in the regulation of H+-ATPase activity. Elevation in endogenous K content enhanced the biosynthesis of hydrogen sulfide (H2S) through enhancing the synthesis of cysteine, the H2S precursor. Synergistic action of H2S and K effectively neutralized osmotic stress by regulating sugar metabolism that resulted in osmotic adjustment, as witnessed by reduced water loss and improved hydration level of the stressed seedlings. Cross talk of H2S and K also assisted the seedlings in the activation of antioxidant enzymes that controlled the generation of reactive oxygen species and led to the protection against oxidative stress. The integrative role of H2S and K signaling was validated using hypotaurine (H2S scavenger) and TEA (K channel blocker) which weakened the protection against osmotic stress induced impairments. In conclusion, exogenous K and endogenous H2S regulate H+-ATPase activity which plays a decisive role in the maintenance of endogenous K homeostasis. Thus, present work reveals that K and H2S crosstalk is essential for modulation of osmotic stress tolerance in tomato seedlings.

Accumulation of dehydrin transcripts and proteins in response to abiotic stresses in Deschampsia antarctica

2004 ◽  
Vol 16 (2) ◽  
pp. 175-184 ◽  
Author(s):  
NÉLIDA OLAVE-CONCHA ◽  
SIMÓN RUIZ-LARA ◽  
XIMENA MUÑOZ ◽  
LEÓN A. BRAVO ◽  
LUIS J. CORCUERA
Keyword(s):  
Low Temperature ◽  
Osmotic Stress ◽  
Vascular Plants ◽  
Ice Nucleation ◽  
Northern Analysis ◽  
Deschampsia Antarctica ◽  
Protein Accumulation ◽  
Exogenous Aba ◽  
Epidermal Tissue ◽  
Dehydrin Gene

Deschampsia antarctica Desv. is one of two vascular plants from the Maritime Antarctic. It is usually exposed to cold, salt, and desiccating winds. We hypothesize that D. antarctica has genes that encode dehydrin proteins and their expression is regulated by low temperature, salt or osmotic stress. To test this hypothesis a fragment of a dehydrin gene from D. antarctica was identified and used as a probe to study dehydrin expression under low temperature, salt, and osmotic stress, and exogenous ABA (abscisic acid) treatments. An anti-dehydrin antibody was also used to study dehydrin protein accumulation under the same treatments. Southern analysis of genomic DNA treated with different endonucleases showed more than four bands recognized by the probe, suggesting that D. antarctica has several dehydrin genes. Northern analysis showed two putative dehydrin transcripts of 1.0 kb accumulated only under exogenous ABA and 1.6 kb under osmotic and salt treatments, suggesting that D. antarctica would have ABA-dependent and - independent pathways for regulation of dehydrin expression. Western analysis showed seven dehydrin proteins (58, 57, 55, 53, 48, 30 and 27 kDa) under the different stress treatments. Cold-accumulated dehydrin proteins were immunolocalized, showing that they are associated with vascular and epidermal tissue, which are preferential ice nucleation zones.

Hydrogen sulfide counteracts chlorophyll loss in sweetpotato seedling leaves and alleviates oxidative damage against osmotic stress

2009 ◽  
Vol 58 (3) ◽  
pp. 243-250 ◽  
Author(s):  
Hua Zhang ◽  
Yong-Kang Ye ◽  
Song-Hua Wang ◽  
Jian-Ping Luo ◽  
Jun Tang ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Osmotic Stress ◽  
Oxidative Damage

scholarly journals Hydrogen peroxide acts as a signal molecule in CO2 laser pretreatment-induced osmotic tolerance in wheat seedling

2011 ◽  
Vol 57 (No. 9) ◽  
pp. 403-408 ◽  
Author(s):  
Z.B. Qiu ◽  
Q. Li ◽  
Z.Z. Bi ◽  
M. Yue
Keyword(s):  
Hydrogen Peroxide ◽  
Osmotic Stress ◽  
Protective Effect ◽  
Stress Responses ◽  
Wheat Seedling ◽  
Physiological Effect ◽  
Signal Molecule ◽  
Wheat Seedlings ◽  
Water Tolerance ◽  
Promotive Effect

The objective of this study was to test whether hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) is involved in laser pretreatment-induced water tolerance in wheat seedlings due to its nature as a second messenger in stress responses. The results showed that 3 min laser pretreatment could enhance water tolerance in wheat seedlings by decreasing the concentration of malondialdehyde (MDA), the production rate of superoxide radical (O<sub>2</sub><sup>&ndash;</sup>), and increasing the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) and the concentration of glutathione (GSH), and having a positive physiological effect on the growth of osmotic stress seedlings. But the promotive effect of laser pretreatment-induced water tolerance in wheat seedling was effectively reversed by addition of 2 mmol AsA (ascorbic acid) or 5 &micro;mol DPI (diphenyle iodonium), but exogenous 100 U/mL CAT could not reversed laser pretreatment-induced protective effect on wheat seedlings under osmotic stress. The results suggest that H<sub>2</sub>O<sub>2</sub> metabolism was involved as signal in the processes of laser-induced water acclimation and laser-induced protective effect was shown to be likely related to NADPH oxidase-dependent H<sub>2</sub>O<sub>2</sub> production.

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