scholarly journals Overexpression of a NF-YC Gene Results in Enhanced Drought and Salt Tolerance in Transgenic Seashore Paspalum

2018 ◽  
Vol 9 ◽  
Author(s):  
Xueli Wu ◽  
Haifan Shi ◽  
Zhenfei Guo
Keyword(s):  
Salt Tolerance ◽  
Seashore Paspalum ◽  
Drought And Salt Tolerance

Co-overexpression of AVP1 and AtNHX1 in Cotton Further Improves Drought and Salt Tolerance in Transgenic Cotton Plants

2014 ◽  
Vol 33 (2) ◽  
pp. 167-177 ◽  
Author(s):  
Guoxin Shen ◽  
Jia Wei ◽  
Xiaoyun Qiu ◽  
Rongbin Hu ◽  
Sundaram Kuppu ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Transgenic Cotton ◽  
Drought And Salt Tolerance ◽  
Cotton Plants

Heterologous Overexpression of ZmHDZIV13 Enhanced Drought and Salt Tolerance in Arabidopsis and Tobacco

2021 ◽  
Author(s):  
Fang Wang ◽  
Peng Fang ◽  
Huiping Yan ◽  
Xiangzhuo Ji ◽  
Yunling Peng
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Salt Tolerance ◽  
Leucine Zipper ◽  
Abiotic Stress Tolerance ◽  
Heterologous Overexpression ◽  
Malondialdehyde Content ◽  
Drought And Salt Tolerance ◽  
Relative Water ◽  
Dependent Signaling Pathway

Abstract The homeodomain leucine zipper (HD-Zip) IV transcription factor is indispensable in the response of plants to abiotic stress. Systematic studies have been carried out in Arabidopsis, rice and other species from which a series of stress resistance-related genes have been isolated. However, the function of the HD-Zip-IV protein in maize is not clear. In this study, we cloned the HD-Zip-IV gene ZmHDZIV13 and identified its function in the stress response. Our phylogenetic analysis showed that ZmHDZIV13 and AtHDG11 had high homology and might have similar functions. The heterologous overexpression of ZmHDZIV13 in Arabidopsis resulted in sensitivity to abscisic acid (ABA), salt tolerance during germination and drought tolerance in seedlings. Under drought stress, the transgenic Arabidopsis showed stronger drought resistance than the wild-type showed (control). The malondialdehyde content of ZmHDZIV13 transgenic plants was lower than that of the control, and the relative water content and proline content were significantly higher than those of the control. After the drought was relieved, the expression of P5CS1, RD22, RD29B, RD29A, NCED3 and ERD1 were upregulated in transgenic Arabidopsis. Also, modified tobacco plants (35S::ZmHDZIV13) exhibited proper stomatal changes in response to drought conditions. These results show that ZmHDZIV13, as a stress-responsive transcription factor, plays a role in the positive regulation of abiotic stress tolerance and can regulate an ABA-dependent signaling pathway to regulate drought response in plants.

scholarly journals Overexpression of the Jojoba Aquaporin Gene, ScPIP1, Enhances Drought and Salt Tolerance in Transgenic Arabidopsis

2019 ◽  
Vol 20 (1) ◽  
pp. 153 ◽  
Author(s):  
Xing Wang ◽  
Fei Gao ◽  
Jie Bing ◽  
Weimin Sun ◽  
Xiuxiu Feng ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Plasma Membranes ◽  
Transgenic Arabidopsis ◽  
Membrane Damage ◽  
Survival Rates ◽  
Transcriptional Level ◽  
Desert Plant ◽  
Ion Leakage ◽  
Plant Tolerance ◽  
Drought And Salt Tolerance

Plasma membrane intrinsic proteins (PIPs) are a subfamily of aquaporin proteins located on plasma membranes where they facilitate the transport of water and small uncharged solutes. PIPs play an important role throughout plant development, and in response to abiotic stresses. Jojoba (Simmondsia chinensis (Link) Schneider), as a typical desert plant, tolerates drought, salinity and nutrient-poor soils. In this study, a PIP1 gene (ScPIP1) was cloned from jojoba and overexpressed in Arabidopsis thaliana. The expression of ScPIP1 at the transcriptional level was induced by polyethylene glycol (PEG) treatment. ScPIP1 overexpressed Arabidopsis plants exhibited higher germination rates, longer roots and higher survival rates compared to the wild-type plants under drought and salt stresses. The results of malonaldehyde (MDA), ion leakage (IL) and proline content measurements indicated that the improved drought and salt tolerance conferred by ScPIP1 was correlated with decreased membrane damage and improved osmotic adjustment. We assume that ScPIP1 may be applied to genetic engineering to improve plant tolerance based on the resistance effect in transgenic Arabidopsis overexpressing ScPIP1.

OsABAR1, a novel GRAM domain-containing protein, confers drought and salt tolerance via an ABA-dependent pathway in rice

2020 ◽  
Vol 152 ◽  
pp. 138-146
Author(s):  
Chongke Zheng ◽  
Jinjun Zhou ◽  
Fang Zhang ◽  
Jingjing Yin ◽  
Guanhua Zhou ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Drought And Salt Tolerance ◽  
Dependent Pathway

scholarly journals Overexpression of a microRNA-targeted NAC transcription factor improves drought and salt tolerance in Rice via ABA-mediated pathways

Rice ◽  
2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Dagang Jiang ◽  
Lingyan Zhou ◽  
Weiting Chen ◽  
Nenghui Ye ◽  
Jixing Xia ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Plant Architecture ◽  
Mutant Gene ◽  
Wild Type ◽  
Drought Treatment ◽  
Stress Regulation ◽  
Qpcr Analysis ◽  
Reverse Transcription Quantitative Pcr ◽  
Drought And Salt Tolerance ◽  
Aba Biosynthesis

Abstract Background The NAC (NAM, AFAT, and CUC) transcription factors play critical roles in rice (Oryza sativa) development and stress regulation. Overexpressing a microRNA (miR164b)-resistant OsNAC2 mutant gene, which generates transcripts that cannot be targeted by miR164b, improves rice plant architecture and yield; however, the performance of these mOsNAC2-overexpressing lines, named ZUOErN3 and ZUOErN4, under abiotic stress conditions such as drought have not yet been fully characterized. Results In this study, we showed that the germination of ZUOErN3 and ZUOErN4 seeds was delayed in comparison with the wild-type (WT) seeds, although the final germination rates of all lines were over 95%. The quantification of the endogenous ABA levels revealed that the germinating mOsNAC2-overexpressing seeds had elevated ABA levels, which resulted in their slower germination. The mOsNAC2-overexpressing plants were significantly more drought tolerance than the WT plants, with the survival rate increasing from 11.2% in the WT to nearly 70% in ZUOErN3 and ZUOErN4 plants after a drought treatment. Salt (NaCl) tolerance was also increased in the ZUOErN3 and ZUOErN4 plants due to significantly increased ABA levels. A reverse transcription quantitative PCR (RT-qPCR) analysis showed a significant increase in the expression of the ABA biosynthesis genes OsNCED1 and OsNCED3 in the mOsNAC2-overexpressing lines, and the expression levels of the stress-responsive genes OsP5CS1, OsLEA3, and OsRab16 were significantly increased in these plants. Moreover, OsNAC2 directly interacted with the promoters of OsLEA3 and OsNCED3 in yeast one-hybrid assays. Conclusions Taken together, our results show that OsNAC2 plays a positive regulatory role in drought and salt tolerance in rice through ABA-mediated pathways.

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