scholarly journals Overexpression of the Jojoba Aquaporin Gene, ScPIP1, Enhances Drought and Salt Tolerance in Transgenic Arabidopsis

2019 ◽  
Vol 20 (1) ◽  
pp. 153 ◽  
Author(s):  
Xing Wang ◽  
Fei Gao ◽  
Jie Bing ◽  
Weimin Sun ◽  
Xiuxiu Feng ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Plasma Membranes ◽  
Transgenic Arabidopsis ◽  
Membrane Damage ◽  
Survival Rates ◽  
Transcriptional Level ◽  
Desert Plant ◽  
Ion Leakage ◽  
Plant Tolerance ◽  
Drought And Salt Tolerance

Plasma membrane intrinsic proteins (PIPs) are a subfamily of aquaporin proteins located on plasma membranes where they facilitate the transport of water and small uncharged solutes. PIPs play an important role throughout plant development, and in response to abiotic stresses. Jojoba (Simmondsia chinensis (Link) Schneider), as a typical desert plant, tolerates drought, salinity and nutrient-poor soils. In this study, a PIP1 gene (ScPIP1) was cloned from jojoba and overexpressed in Arabidopsis thaliana. The expression of ScPIP1 at the transcriptional level was induced by polyethylene glycol (PEG) treatment. ScPIP1 overexpressed Arabidopsis plants exhibited higher germination rates, longer roots and higher survival rates compared to the wild-type plants under drought and salt stresses. The results of malonaldehyde (MDA), ion leakage (IL) and proline content measurements indicated that the improved drought and salt tolerance conferred by ScPIP1 was correlated with decreased membrane damage and improved osmotic adjustment. We assume that ScPIP1 may be applied to genetic engineering to improve plant tolerance based on the resistance effect in transgenic Arabidopsis overexpressing ScPIP1.

scholarly journals A Novel Sweetpotato WRKY Transcription Factor, IbWRKY2, Positively Regulates Drought and Salt Tolerance in Transgenic Arabidopsis

Biomolecules ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 506 ◽  
Author(s):  
Hong Zhu ◽  
Yuanyuan Zhou ◽  
Hong Zhai ◽  
Shaozhen He ◽  
Ning Zhao ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Ipomoea Batatas ◽  
Transgenic Arabidopsis ◽  
Abiotic Stress Tolerance ◽  
Wrky Transcription Factor ◽  
Oxygen Species ◽  
Aba Signaling ◽  
Ros Scavenging ◽  
Drought And Salt Tolerance ◽  
Transgenic Lines

WRKYs play important roles in plant growth, defense regulation, and stress response. However, the mechanisms through which WRKYs are involved in drought and salt tolerance have been rarely characterized in sweetpotato [Ipomoea batatas (L.) Lam.]. In this study, we cloned a WRKY gene, IbWRKY2, from sweetpotato and its expression was induced with PEG6000, NaCl, and abscisic acid (ABA). The IbWRKY2 was localized in the nucleus. The full-length protein exhibited transactivation activity, and its active domain was located in the N-terminal region. IbWRKY2-overexpressing Arabidopsis showed enhanced drought and salt tolerance. After drought and salt treatments, the contents of ABA and proline as well as the activity of superoxide dismutase (SOD) were higher in transgenic plants, while the malondialdehyde (MDA) and H2O2 contents were lower. In addition, several genes related to the ABA signaling pathway, proline biosynthesis, and the reactive oxygen species (ROS)-scavenging system, were significantly up-regulated in transgenic lines. These results demonstrate that IbWRKY2 confers drought and salt tolerance in Arabidopsis. Furthermore, IbWRKY2 was able to interact with IbVQ4, and the expression of IbVQ4 was induced by drought and salt treatments. These results provide clues regarding the mechanism by which IbWRKY2 contributes to the regulation of abiotic stress tolerance.

Cloning and characterization of a maize bZIP transcription factor, ZmbZIP72, confers drought and salt tolerance in transgenic Arabidopsis

Planta ◽  
2011 ◽  
Vol 235 (2) ◽  
pp. 253-266 ◽  
Author(s):  
Sheng Ying ◽  
Deng-Feng Zhang ◽  
Jing Fu ◽  
Yun-Su Shi ◽  
Yan-Chun Song ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Salt Tolerance ◽  
Transgenic Arabidopsis ◽  
Bzip Transcription Factor ◽  
Drought And Salt Tolerance
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