scholarly journals Dissecting the Subcellular Localization, Intracellular Trafficking, Interactions, Membrane Association, and Topology of Citrus Leprosis Virus C Proteins

2018 ◽  
Vol 9 ◽  
Author(s):  
Mikhail Oliveira Leastro ◽  
Elliot Watanabe Kitajima ◽  
Marilia Santos Silva ◽  
Renato Oliveira Resende ◽  
Juliana Freitas-Astúa
Keyword(s):  
Subcellular Localization ◽  
Intracellular Trafficking ◽  
Membrane Association ◽  
And Topology ◽  
Citrus Leprosis ◽  
C Proteins ◽  
Virus C

scholarly journals Membrane Association and Topology of Citrus Leprosis Virus C2 Movement and Capsid Proteins

2021 ◽  
Vol 9 (2) ◽  
pp. 418
Author(s):  
Mikhail Oliveira Leastro ◽  
Juliana Freitas-Astúa ◽  
Elliot Watanabe Kitajima ◽  
Vicente Pallás ◽  
Jesús Á. Sánchez-Navarro
Keyword(s):  
Capsid Protein ◽  
Movement Protein ◽  
Capsid Proteins ◽  
Membrane Association ◽  
Membrane Fractionation ◽  
Chemical Treatments ◽  
And Topology ◽  
Intracellular Movement ◽  
Citrus Leprosis ◽  
Biomolecular Fluorescence Complementation

Although citrus leprosis disease has been known for more than a hundred years, one of its causal agents, citrus leprosis virus C2 (CiLV-C2), is poorly characterized. This study described the association of CiLV-C2 movement protein (MP) and capsid protein (p29) with biological membranes. Our findings obtained by computer predictions, chemical treatments after membrane fractionation, and biomolecular fluorescence complementation assays revealed that p29 is peripherally associated, while the MP is integrally bound to the cell membranes. Topological analyses revealed that both the p29 and MP expose their N- and C-termini to the cell cytoplasmic compartment. The implications of these results in the intracellular movement of the virus were discussed.

scholarly journals Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity

2020 ◽  
Vol 11 ◽  
Author(s):  
Mikhail Oliveira Leastro ◽  
Deibis Yorlenis Ortega Castro ◽  
Juliana Freitas-Astúa ◽  
Elliot Watanabe Kitajima ◽  
Vicente Pallás ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Citrus Leprosis ◽  
Silencing Suppression ◽  
Virus C

scholarly journals First Report of Brevipalpus papayensis as Vector of Coffee ringspot virus and Citrus leprosis virus C

Plant Disease ◽  
2018 ◽  
Vol 102 (5) ◽  
pp. 1046-1046 ◽  
Author(s):  
M. A. Nunes ◽  
J. L. de Carvalho Mineiro ◽  
L. A. Rogerio ◽  
L. M. Ferreira ◽  
A. Tassi ◽  
...  
Keyword(s):  
Ringspot Virus ◽  
First Report ◽  
Citrus Leprosis ◽  
Virus C

Tropical spiderwort (Commelina benghalensis L.) as source of inoculum for Citrus leprosis virus C

2012 ◽  
Vol 33 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Maria Andréia Nunes ◽  
Paulo Lameiro ◽  
Renata Faier Calegario ◽  
Marcela P. Bergamini ◽  
Luciane Fender Coerini ◽  
...  
Keyword(s):  
Commelina Benghalensis ◽  
Citrus Leprosis ◽  
Virus C

scholarly journals The subcellular localization of the Niemann-Pick Type C proteins depends on the adaptor complex AP-3

2007 ◽  
Vol 120 (20) ◽  
pp. 3640-3652 ◽  
Author(s):  
A. C. Berger ◽  
G. Salazar ◽  
M. L. Styers ◽  
K. A. Newell-Litwa ◽  
E. Werner ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Type C ◽  
C Proteins ◽  
Niemann Pick

Sendai virus C proteins are categorically nonessential gene products but silencing their expression severely impairs viral replication and pathogenesis

1998 ◽  
Vol 3 (2) ◽  
pp. 111-124 ◽  
Author(s):  
Atsushi Kurotani ◽  
Katsuhiro Kiyotani ◽  
Atsushi Kato ◽  
Tatsuo Shioda ◽  
Yuko Sakai ◽  
...  
Keyword(s):  
Viral Replication ◽  
Sendai Virus ◽  
Gene Products ◽  
Nonessential Gene ◽  
C Proteins ◽  
Virus C

scholarly journals Spontaneous Mutation in the Movement Protein of Citrus Leprosis Virus C2, in a Heterologous Virus Infection Context, Increases Cell-to-Cell Transport and Generates Fitness Advantage

Viruses ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2498
Author(s):  
Mikhail Oliveira Leastro ◽  
David Villar-Álvarez ◽  
Juliana Freitas-Astúa ◽  
Elliot Watanabe Kitajima ◽  
Vicente Pallás ◽  
...  
Keyword(s):  
Movement Protein ◽  
Spontaneous Mutation ◽  
Alfalfa Mosaic Virus ◽  
Viral Fitness ◽  
Viral Population ◽  
Long Distance ◽  
Cell Transport ◽  
Fitness Advantage ◽  
Citrus Leprosis ◽  
Virus C

Previous results using a movement defective alfalfa mosaic virus (AMV) vector revealed that citrus leprosis virus C (CiLV-C) movement protein (MP) generates a more efficient local movement, but not more systemic transport, than citrus leprosis virus C2 (CiLV-C2) MP, MPs belonging to two important viruses for the citrus industry. Here, competition experiment assays in transgenic tobacco plants (P12) between transcripts of AMV constructs expressing the cilevirus MPs, followed by several biological passages, showed the prevalence of the AMV construct carrying the CiLV-C2 MP. The analysis of AMV RNA 3 progeny recovered from P12 plant at the second viral passage revealed the presence of a mix of progeny encompassing the CiLV-C2 MP wild type (MPWT) and two variants carrying serines instead phenylalanines at positions 72 (MPS72F) or 259 (MPS259F), respectively. We evaluated the effects of each modified residue in virus replication, and cell-to-cell and long-distance movements. Results indicated that phenylalanine at position 259 favors viral cell-to-cell transport with an improvement in viral fitness, but has no effect on viral replication, whereas mutation at position 72 (MPS72F) has a penalty in the viral fitness. Our findings indicate that the prevalence of a viral population may be correlated with its greater efficiency in cell-to-cell and systemic movements.

Citrus leprosis virus C. [Distribution map].

2013 ◽  
Author(s):  
Keyword(s):  
Central America ◽  
Citrus Sinensis ◽  
Rio Grande Do Sul ◽  
Distribution Map ◽  
Mato Grosso ◽  
C Distribution ◽  
Citrus Leprosis ◽  
Virus C ◽  
Mato Grosso Do Sul ◽  
New Distribution

Abstract A new distribution map is provided for Citrus leprosis virus C. Cilevirus. Hosts: orange (Citrus sinensis) and other Citrus spp. Information is given on the geographical distribution in North America (Mexico), Central America & Caribbean (Belize, Costa Rica, El Salvador, Guatemala, Honduras, Nicaragua, Panama), South America (Argentina, Bolivia, Brazil, Acre, Amazonas, Bahia, Ceara, Espirito Santo, Goias, Mato Grosso, Mato Grosso do Sul, Minas Gerais, Para, Parana, Piaui, Rio de Janeiro, Rio Grande do Sul, Rondonia, Roraima, Santa Catarina, Sao Paulo, Sergipe, Tocantins, Colombia, Paraguay, Uruguay, Venezuela).

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