scholarly journals Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity

2020 ◽  
Vol 11 ◽  
Author(s):  
Mikhail Oliveira Leastro ◽  
Deibis Yorlenis Ortega Castro ◽  
Juliana Freitas-Astúa ◽  
Elliot Watanabe Kitajima ◽  
Vicente Pallás ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Citrus Leprosis ◽  
Silencing Suppression ◽  
Virus C

scholarly journals Investigation of the effects of P1 on HC-pro-mediated gene silencing suppression through genetics and omics approaches

2020 ◽  
Vol 61 (1) ◽  
Author(s):  
Sin-Fen Hu ◽  
Wei-Lun Wei ◽  
Syuan-Fei Hong ◽  
Ru-Ying Fang ◽  
Hsin-Yi Wu ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Silencing Suppression

scholarly journals First Report of Brevipalpus papayensis as Vector of Coffee ringspot virus and Citrus leprosis virus C

Plant Disease ◽  
2018 ◽  
Vol 102 (5) ◽  
pp. 1046-1046 ◽  
Author(s):  
M. A. Nunes ◽  
J. L. de Carvalho Mineiro ◽  
L. A. Rogerio ◽  
L. M. Ferreira ◽  
A. Tassi ◽  
...  
Keyword(s):  
Ringspot Virus ◽  
First Report ◽  
Citrus Leprosis ◽  
Virus C

Tropical spiderwort (Commelina benghalensis L.) as source of inoculum for Citrus leprosis virus C

2012 ◽  
Vol 33 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Maria Andréia Nunes ◽  
Paulo Lameiro ◽  
Renata Faier Calegario ◽  
Marcela P. Bergamini ◽  
Luciane Fender Coerini ◽  
...  
Keyword(s):  
Commelina Benghalensis ◽  
Citrus Leprosis ◽  
Virus C

scholarly journals Spontaneous Mutation in the Movement Protein of Citrus Leprosis Virus C2, in a Heterologous Virus Infection Context, Increases Cell-to-Cell Transport and Generates Fitness Advantage

Viruses ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2498
Author(s):  
Mikhail Oliveira Leastro ◽  
David Villar-Álvarez ◽  
Juliana Freitas-Astúa ◽  
Elliot Watanabe Kitajima ◽  
Vicente Pallás ◽  
...  
Keyword(s):  
Movement Protein ◽  
Spontaneous Mutation ◽  
Alfalfa Mosaic Virus ◽  
Viral Fitness ◽  
Viral Population ◽  
Long Distance ◽  
Cell Transport ◽  
Fitness Advantage ◽  
Citrus Leprosis ◽  
Virus C

Previous results using a movement defective alfalfa mosaic virus (AMV) vector revealed that citrus leprosis virus C (CiLV-C) movement protein (MP) generates a more efficient local movement, but not more systemic transport, than citrus leprosis virus C2 (CiLV-C2) MP, MPs belonging to two important viruses for the citrus industry. Here, competition experiment assays in transgenic tobacco plants (P12) between transcripts of AMV constructs expressing the cilevirus MPs, followed by several biological passages, showed the prevalence of the AMV construct carrying the CiLV-C2 MP. The analysis of AMV RNA 3 progeny recovered from P12 plant at the second viral passage revealed the presence of a mix of progeny encompassing the CiLV-C2 MP wild type (MPWT) and two variants carrying serines instead phenylalanines at positions 72 (MPS72F) or 259 (MPS259F), respectively. We evaluated the effects of each modified residue in virus replication, and cell-to-cell and long-distance movements. Results indicated that phenylalanine at position 259 favors viral cell-to-cell transport with an improvement in viral fitness, but has no effect on viral replication, whereas mutation at position 72 (MPS72F) has a penalty in the viral fitness. Our findings indicate that the prevalence of a viral population may be correlated with its greater efficiency in cell-to-cell and systemic movements.

scholarly journals Investigation of the effects of P1 on HC-Pro-mediated gene silencing suppression through genetics and omics approaches

2020 ◽  
Author(s):  
Sin-Fen Hu ◽  
Wei-Lun Wei ◽  
Syuan-Fei Hong ◽  
Ru-Ying Fang ◽  
Hsin-Yi Wu ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Rna Degradation ◽  
Novel Genes ◽  
Posttranscriptional Gene Silencing ◽  
Comparative Network Analysis ◽  
New Findings ◽  
A Subunit ◽  
Silencing Suppression ◽  
Viral Suppressors ◽  
Interfering Rna

Abstract Background: Posttranscriptional gene silencing (PTGS) is one of the most important mechanisms for plants during viral infection. However, viruses have also developed viral suppressors to negatively control PTGS by inhibiting microRNA (miRNA) and short-interfering RNA (siRNA) regulation in plants. The first identified viral suppressor, P1/HC-Pro, is a fusion protein. Upon infecting plants, the P1 protein itself is released from HC-Pro by the self-cleaving activity of P1. P1 has an unknown function in enhancing HC-Pro-mediated PTGS suppression. We performed proteomics to identify P1-interacting proteins. We also performed transcriptomics that were generated from Col-0 and various P1/HC-Pro-related transgenic plants to identify novel genes. The results showed several novel genes were identified through the comparative network analysis that might be involved in P1/HC-Pro-mediated PTGS suppression. Results: First, we demonstrated that P1 enhances HC-Pro function and that the mechanism might work through P1 binding to VERNALIZATION INDEPENDENCE 3/SUPERKILLER 8 (VIP3/SKI8), a subunit of the exosome, to interfere with the 5'-fragment of the PTGS-cleaved RNA degradation product. Second, specifically the AGO1 was specifically posttranslationally degraded in transgenic Arabidopsis expressing P1/HC-Pro of turnip mosaic virus (TuMV) (P1/HCTu plant). Third, the comparative network highlighted potentially critical genes in PTGS, including miRNA targets, calcium signaling, hormone (JA, ET, and ABA) signaling, and defense response. Conclusion: Through these genetic and omics approaches, we revealed an overall perspective to identify many critical genes involved in PTGS. These new findings significantly impact in our understanding of P1/HC-Pro-mediated PTGS suppression.

Citrus leprosis virus C. [Distribution map].

2013 ◽  
Author(s):  
Keyword(s):  
Central America ◽  
Citrus Sinensis ◽  
Rio Grande Do Sul ◽  
Distribution Map ◽  
Mato Grosso ◽  
C Distribution ◽  
Citrus Leprosis ◽  
Virus C ◽  
Mato Grosso Do Sul ◽  
New Distribution

Abstract A new distribution map is provided for Citrus leprosis virus C. Cilevirus. Hosts: orange (Citrus sinensis) and other Citrus spp. Information is given on the geographical distribution in North America (Mexico), Central America & Caribbean (Belize, Costa Rica, El Salvador, Guatemala, Honduras, Nicaragua, Panama), South America (Argentina, Bolivia, Brazil, Acre, Amazonas, Bahia, Ceara, Espirito Santo, Goias, Mato Grosso, Mato Grosso do Sul, Minas Gerais, Para, Parana, Piaui, Rio de Janeiro, Rio Grande do Sul, Rondonia, Roraima, Santa Catarina, Sao Paulo, Sergipe, Tocantins, Colombia, Paraguay, Uruguay, Venezuela).

Citrus leprosis virus C (leprosis of citrus).

2021 ◽  
Author(s):  
John S. Hartung ◽  
M. Guillermo León
Keyword(s):  
Systemic Infection ◽  
Economic Losses ◽  
American Continent ◽  
Major Pathway ◽  
The Past ◽  
Planting Material ◽  
Latently Infected ◽  
Citrus Leprosis ◽  
Virus C ◽  
Important Disease

Abstract CiLV-C is a quarantine pest which causes an economically important disease, reported only on the American continent. During the past 15 years, it has caused economic losses in Brazil, Argentina, Paraguay, Uruguay, Venezuela, Costa Rica, Panamá and Honduras. The disease was recently reported in Guatemala, Bolivia, México, Colombia and Belize. It is a threat to citrus-producing countries where the disease has not been reported. The disease can cause 100% yield loss (Rodrigues et al., 2000). CiLV does not appear to move systemically in the host plant but can move short distances from a grafted shoot tip to the adjacent scion tissue. Accordingly, movement in latently infected planting material is not likely to be a major pathway for CiLV-C because of its non-systemic infection, CiLV-C can only be important where attacks by its vector mites are significant. The main means of movement and dispersal of the virus is via the vector mites of the genus Brevipalpus, which colonize most species of Citrus and many other plant species.

scholarly journals Transmission of Citrus leprosis virus C by Brevipalpus phoenicis (Geijskes) to Alternative Host Plants Found in Citrus Orchards

Plant Disease ◽  
2012 ◽  
Vol 96 (7) ◽  
pp. 968-972 ◽  
Author(s):  
M. A. Nunes ◽  
C. A. L. de Oliveira ◽  
M. L. de Oliveira ◽  
E. W. Kitajima ◽  
M. E. Hilf ◽  
...  
Keyword(s):  
Host Plants ◽  
Sweet Orange ◽  
Hibiscus Rosa Sinensis ◽  
Brevipalpus Phoenicis ◽  
Orange Fruit ◽  
Necrotic Spots ◽  
Citrus Orchards ◽  
Citrus Leprosis ◽  
Polymerase Chain ◽  
Virus C

The equivalent of US$75 million is spent each year in Brazil to control Brevipalpus phoenicis, a mite vector of Citrus leprosis virus C (CiLV-C). In this study, we investigated the possibility that hedgerows and windbreaks normally found in citrus orchards could host CiLV-C. Mites confined by an adhesive barrier were reared on sweet orange fruit with leprosis symptoms then were transferred to leaves of Hibiscus rosa-sinensis, Malvaviscus arboreus, Grevilea robusta, Bixa orellana, and Citrus sinensis. Ninety days post infestation, the descendant mites were transferred to Pera sweet orange plants to verify the transmissibility of the virus back to citrus. Nonviruliferous mites which had no feeding access to diseased tissue were used as controls. Local chlorotic or necrotic spots and ringspots, symptoms of leprosis disease, appeared in most plants tested. Results generated by reversetranscription polymerase chain reaction with primers specific for CiLV-C and by electron microscope analyses confirmed the susceptibility of these plants to CiLV-C.

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