scholarly journals A Mini Zinc-Finger Protein (MIF) from Gerbera hybrida Activates the GASA Protein Family Gene, GEG, to Inhibit Ray Petal Elongation

2017 ◽  
Vol 8 ◽  
Author(s):  
Meixiang Han ◽  
Xuefeng Jin ◽  
Wei Yao ◽  
Lingjie Kong ◽  
Gan Huang ◽  
...  
Author(s):  
Lu Xue ◽  
Hongling Qiu ◽  
Jian Ma ◽  
Mingxiong Guo ◽  
Wenxin Li

AbstractZNF300 was recently identified as a member of the human KRAB/C2H2 zinc finger protein family. Little is known about the role of ZNF300 in human gene regulation networks. In this study, the DNA-binding property of ZNF300 was further analyzed. We found that the recombinant ZNF300 could bind to the binding site 5′-GCGGGGGCG-3′ of Egr1, another member of the KRAB/C2H2 zinc finger protein family. Similarly, recombinant Egr1 also showed a similar binding affinity to the ZNF300 binding site 5′-CTGGGGGCG-3′. Bioinformatics analysis revealed that there is an overlapping ZNF300/Egr1 binding site in the human IL-2Rβ promoter region, which was previously known to be recognized by endogenous Egr1. Electrophoretic mobility shift assays showed that endogenous ZNF300 could also bind to this site. A transient transfection assay revealed that both ZNF300 and Egr1 could transactivate the IL-2Rβ promoter, and that the activation was abrogated by a mutation of residues in the overlapping ZNF300/Egr1 binding site. Co-expression of ZNF300 and Egr1 led to enhanced IL-2Rβ promoter activity. Thus, ZNF300 is likely to be another regulator of the human IL-2Rβ promoter.


BMC Genomics ◽  
2006 ◽  
Vol 7 (1) ◽  
Author(s):  
Joseph Colasanti ◽  
Reynald Tremblay ◽  
Ada YM Wong ◽  
Viktoriya Coneva ◽  
Akiko Kozaki ◽  
...  

2012 ◽  
Vol 77 (3) ◽  
pp. 217-226 ◽  
Author(s):  
S. V. Razin ◽  
V. V. Borunova ◽  
O. G. Maksimenko ◽  
O. L. Kantidze

1998 ◽  
Vol 26 (2) ◽  
pp. 608-615 ◽  
Author(s):  
K.-i. Kubo ◽  
A. Sakamoto ◽  
A. Kobayashi ◽  
Z. Rybka ◽  
Y. Kanno ◽  
...  

2001 ◽  
Vol 15 (10) ◽  
pp. 1693-1705 ◽  
Author(s):  
Tetsuya Mizutani ◽  
Kazuya Yamada ◽  
Takashi Yazawa ◽  
Toshinori Okada ◽  
Takashi Minegishi ◽  
...  

2001 ◽  
Vol 101 (1-2) ◽  
pp. 203-207 ◽  
Author(s):  
Thomas Ott ◽  
Mark Parrish ◽  
Kristin Bond ◽  
Anja Schwaeger-Nickolenko ◽  
A.Paula Monaghan

Gene ◽  
2007 ◽  
Vol 403 (1-2) ◽  
pp. 125-131 ◽  
Author(s):  
Lina Medugno ◽  
Francesca Florio ◽  
Elena Cesaro ◽  
Michela Grosso ◽  
Angelo Lupo ◽  
...  

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