scholarly journals Cys2/His2 zinc-finger protein family of petunia: Evolution and general mechanism of target-sequence recognition

1998 ◽  
Vol 26 (2) ◽  
pp. 608-615 ◽  
Author(s):  
K.-i. Kubo ◽  
A. Sakamoto ◽  
A. Kobayashi ◽  
Z. Rybka ◽  
Y. Kanno ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Protein Family ◽  
General Mechanism ◽  
Target Sequence ◽  
Sequence Recognition ◽  
Finger Protein

scholarly journals ZNF300, a recently identified human transcription factor, activates the human IL-2Rβ promoter through the overlapping ZNF300/EGR1 binding site

2010 ◽  
Vol 15 (4) ◽  
Author(s):  
Lu Xue ◽  
Hongling Qiu ◽  
Jian Ma ◽  
Mingxiong Guo ◽  
Wenxin Li
Keyword(s):  
Binding Site ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Protein Family ◽  
Binding Property ◽  
Mobility Shift ◽  
Transient Transfection Assay ◽  
C2h2 Zinc Finger ◽  
Finger Protein ◽  
C2h2 Zinc Finger Protein

AbstractZNF300 was recently identified as a member of the human KRAB/C2H2 zinc finger protein family. Little is known about the role of ZNF300 in human gene regulation networks. In this study, the DNA-binding property of ZNF300 was further analyzed. We found that the recombinant ZNF300 could bind to the binding site 5′-GCGGGGGCG-3′ of Egr1, another member of the KRAB/C2H2 zinc finger protein family. Similarly, recombinant Egr1 also showed a similar binding affinity to the ZNF300 binding site 5′-CTGGGGGCG-3′. Bioinformatics analysis revealed that there is an overlapping ZNF300/Egr1 binding site in the human IL-2Rβ promoter region, which was previously known to be recognized by endogenous Egr1. Electrophoretic mobility shift assays showed that endogenous ZNF300 could also bind to this site. A transient transfection assay revealed that both ZNF300 and Egr1 could transactivate the IL-2Rβ promoter, and that the activation was abrogated by a mutation of residues in the overlapping ZNF300/Egr1 binding site. Co-expression of ZNF300 and Egr1 led to enhanced IL-2Rβ promoter activity. Thus, ZNF300 is likely to be another regulator of the human IL-2Rβ promoter.

scholarly journals The maize INDETERMINATE1 flowering time regulator defines a highly conserved zinc finger protein family in higher plants

BMC Genomics ◽  
2006 ◽  
Vol 7 (1) ◽  
Author(s):  
Joseph Colasanti ◽  
Reynald Tremblay ◽  
Ada YM Wong ◽  
Viktoriya Coneva ◽  
Akiko Kozaki ◽  
...  
Keyword(s):  
Flowering Time ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Higher Plants ◽  
Protein Family ◽  
Finger Protein

Cys2His2 zinc finger protein family: Classification, functions, and major members

2012 ◽  
Vol 77 (3) ◽  
pp. 217-226 ◽  
Author(s):  
S. V. Razin ◽  
V. V. Borunova ◽  
O. G. Maksimenko ◽  
O. L. Kantidze
Keyword(s):  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Protein Family ◽  
Finger Protein ◽  
Family Classification

The artificial 4-zinc-finger protein Bagly binds human utrophin promoter A at the endogenous chromosomal site and activates transcription

2007 ◽  
Vol 85 (3) ◽  
pp. 358-365 ◽  
Author(s):  
Annalisa Onori ◽  
Agata Desantis ◽  
Serena Buontempo ◽  
Maria Grazia Di Certo ◽  
Maurizio Fanciulli ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Transcriptional Activity ◽  
Zinc Finger Protein ◽  
Target Sequence ◽  
Zinc Finger Domain ◽  
Active Chromatin ◽  
Finger Protein ◽  
Chromosomal Site ◽  
Transcription Factor Yy1 ◽  
Test Gene

Our aim is to upregulate the expression of the dystrophin-related gene utrophin in Duchenne muscular dystrophy, in this way complementing the lack of dystrophin function. To achieve utrophin upregulation, we designed and engineered synthetic zinc-inger based transcription factors. We have previously shown that the artificial 3-zinc-finger protein Jazz, fused with the appropriate effector domain, is able to drive the transcription of a test gene from utrophin promoter A. Here we report a novel artificial 4-zinc-finger protein, Bagly, which binds with optimized affinity–specificity to a 12 bp DNA target sequence that is internal to human utrophin promoter A. Bagly was generated adding to Jazz protein an extra-fourth zinc finger, derived from transcription factor YY1. Importantly, the Bagly DNA target sequence is statistically present in the human genome only 210 times, about 60 fewer times than the 9 bp Jazz DNA target sequence. Thanks to its additional zinc-finger domain, Bagly protein shows enhanced transcriptional activity. Moreover, we demonstrated Bagly's effective access and binding to active chromatin in the chromosomal context and its ability to upregulate endogenous utrophin.

Cloning and Characterization of Gonadotropin-Inducible Ovarian Transcription Factors (GIOT1 and -2) That Are Novel Members of the (Cys)2-(His)2-Type Zinc Finger Protein Family

2001 ◽  
Vol 15 (10) ◽  
pp. 1693-1705 ◽  
Author(s):  
Tetsuya Mizutani ◽  
Kazuya Yamada ◽  
Takashi Yazawa ◽  
Toshinori Okada ◽  
Takashi Minegishi ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Protein Family ◽  
Finger Protein

scholarly journals A new member of the spalt like zinc finger protein family, Msal-3, is expressed in the CNS and sites of epithelial/mesenchymal interaction

2001 ◽  
Vol 101 (1-2) ◽  
pp. 203-207 ◽  
Author(s):  
Thomas Ott ◽  
Mark Parrish ◽  
Kristin Bond ◽  
Anja Schwaeger-Nickolenko ◽  
A.Paula Monaghan
Keyword(s):  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Protein Family ◽  
Finger Protein

Differential expression and cellular localization of ZNF224 and ZNF255, two isoforms of the Krüppel-like zinc-finger protein family

Gene ◽  
2007 ◽  
Vol 403 (1-2) ◽  
pp. 125-131 ◽  
Author(s):  
Lina Medugno ◽  
Francesca Florio ◽  
Elena Cesaro ◽  
Michela Grosso ◽  
Angelo Lupo ◽  
...  
Keyword(s):  
Differential Expression ◽  
Zinc Finger ◽  
Cellular Localization ◽  
Zinc Finger Protein ◽  
Protein Family ◽  
Finger Protein
Sign in / Sign up

Export Citation Format

Share Document