scholarly journals Transcriptome Analysis of Genes Associated with the Artemisinin Biosynthesis by Jasmonic Acid Treatment under the Light in Artemisia annua

2017 ◽  
Vol 8 ◽  
Author(s):  
Xiaolong Hao ◽  
Yijun Zhong ◽  
Xueqing Fu ◽  
Zongyou Lv ◽  
Qian Shen ◽  
...  
Keyword(s):  
Jasmonic Acid ◽  
Transcriptome Analysis ◽  
Acid Treatment ◽  
Artemisia Annua ◽  
Artemisinin Biosynthesis

Jasmonic acid‐responsive AabHLH1 positively regulates artemisinin biosynthesis in Artemisia annua

2019 ◽  
Vol 66 (3) ◽  
pp. 369-375 ◽  
Author(s):  
Ling Li ◽  
Xiaolong Hao ◽  
Hang Liu ◽  
Wei Wang ◽  
Xueqing Fu ◽  
...  
Keyword(s):  
Jasmonic Acid ◽  
Artemisia Annua ◽  
Artemisinin Biosynthesis

The molecular cloning of dihydroartemisinic aldehyde reductase and its implication in artemisinin biosynthesis in Artemisia annua

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
O Kayser ◽  
A Ryden ◽  
H Bouwmeester ◽  
C Ruyter Spira ◽  
H Osada ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Artemisia Annua ◽  
Aldehyde Reductase ◽  
Artemisinin Biosynthesis

Agrobacteium Transformation of Tobacco with a Genetic Module of Antimalarial Agent Artemisinin Biosynthesis

2020 ◽  
Vol 36 (3) ◽  
pp. 34-45
Author(s):  
T.Yu. Mitiuchkina ◽  
A.S. Pushin ◽  
A.K. Tzareva ◽  
A.M. Vainstein ◽  
S.V. Dolgov
Keyword(s):  
Target Genes ◽  
Artemisia Annua ◽  
Biosynthesis Pathway ◽  
Expression Systems ◽  
Heterologous Host ◽  
Russian Science ◽  
Tobacco Plants ◽  
Genetic Module ◽  
Heterologous Expression Systems ◽  
Artemisinin Biosynthesis

Artemisinin-based medicines are the most effective treatment for malaria. To date, the wormwood plants (Artemisia annua L.) are the main source of artemisinin. Due to the limited nature of this source, considerable efforts are directed towards the development of methods for artemisinin production via heterologous expression systems. We used in this study agrobacterial transformation to transfer the genetic module of the artemisinin biosynthesis pathway into plants and then analyzed its transcription in a heterologous host. Tobacco plants were transformed with the artemisinin biosynthesis genes encoding amorpha-4,11-diene synthase, artemisin-aldehyde All(13) reductase, amorpha-4,11-diene monooxygenase, cytochrome P450 reductase from A. annua and yeast 3-hydroxy-3-methylglutaryl-coenzyme A reductase cloned in the pArtemC vector; farnesyl diphosphate synthase and aldehyde dehydrogenase were used to transform the plants as parts of vector p2356. As a result of transformation with the pArtemC and p2356 vectors, in twos transgenic lines with all target genes were obtained. Five genes of artemisinin biosynthesis and two genes of biosynthesis of its precursors were successfully transferred into the genome of transgenic tobacco lines as a result of the co-transformation with abovementioned vectors. Thus, the entire artemisinin biosynthesis pathway was first reconstructed in heterologous plants: the transcription of the artemisinin biosynthesis genes in the tobacco plants was shown via RT-PCR. The obtained results will be used in further research on expression systems for the production of artemisinin and other non-protein substances in heterologous host plants. artemisinin, malaria, metabolic engineering, tobacco, transgenic plants This work was supported by a Grant from the Russian Science Foundation no. 19-14-00190.

Arbuscular mycorrhiza increase artemisinin accumulation in Artemisia annua by higher expression of key biosynthesis genes via enhanced jasmonic acid levels

Mycorrhiza ◽  
2014 ◽  
Vol 25 (5) ◽  
pp. 345-357 ◽  
Author(s):  
Shantanu Mandal ◽  
Shivangi Upadhyay ◽  
Saima Wajid ◽  
Mauji Ram ◽  
Dharam Chand Jain ◽  
...  
Keyword(s):  
Jasmonic Acid ◽  
Arbuscular Mycorrhiza ◽  
Artemisia Annua

scholarly journals Postharvest jasmonic acid treatment of sugarbeet roots reduces rot due to Botrytis cinerea, Penicillium claviforme, and Phoma betae

2012 ◽  
Vol 65 ◽  
pp. 1-4 ◽  
Author(s):  
Karen Klotz Fugate ◽  
Jocleita Peruzzo Ferrareze ◽  
Melvin D. Bolton ◽  
Edward L. Deckard ◽  
Larry G. Campbell
Keyword(s):  
Jasmonic Acid ◽  
Botrytis Cinerea ◽  
Acid Treatment ◽  
Penicillium Claviforme ◽  
Phoma Betae

scholarly journals The physiological response of Artemisia annua L. to salt stress and salicylic acid treatment

2014 ◽  
Vol 20 (2) ◽  
pp. 161-169 ◽  
Author(s):  
Lin Li ◽  
Haihui Zhang ◽  
Li Zhang ◽  
Yonghong Zhou ◽  
Ruiwu Yang ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Salt Stress ◽  
Physiological Response ◽  
Acid Treatment ◽  
Artemisia Annua ◽  
Salicylic Acid Treatment ◽  
Artemisia Annua L
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