Jasmonic acid‐responsive AabHLH1 positively regulates artemisinin biosynthesis in Artemisia annua

2019 ◽  
Vol 66 (3) ◽  
pp. 369-375 ◽  
Author(s):  
Ling Li ◽  
Xiaolong Hao ◽  
Hang Liu ◽  
Wei Wang ◽  
Xueqing Fu ◽  
...  
Keyword(s):  
Jasmonic Acid ◽  
Artemisia Annua ◽  
Artemisinin Biosynthesis

The molecular cloning of dihydroartemisinic aldehyde reductase and its implication in artemisinin biosynthesis in Artemisia annua

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
O Kayser ◽  
A Ryden ◽  
H Bouwmeester ◽  
C Ruyter Spira ◽  
H Osada ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Artemisia Annua ◽  
Aldehyde Reductase ◽  
Artemisinin Biosynthesis

Agrobacteium Transformation of Tobacco with a Genetic Module of Antimalarial Agent Artemisinin Biosynthesis

2020 ◽  
Vol 36 (3) ◽  
pp. 34-45
Author(s):  
T.Yu. Mitiuchkina ◽  
A.S. Pushin ◽  
A.K. Tzareva ◽  
A.M. Vainstein ◽  
S.V. Dolgov
Keyword(s):  
Target Genes ◽  
Artemisia Annua ◽  
Biosynthesis Pathway ◽  
Expression Systems ◽  
Heterologous Host ◽  
Russian Science ◽  
Tobacco Plants ◽  
Genetic Module ◽  
Heterologous Expression Systems ◽  
Artemisinin Biosynthesis

Artemisinin-based medicines are the most effective treatment for malaria. To date, the wormwood plants (Artemisia annua L.) are the main source of artemisinin. Due to the limited nature of this source, considerable efforts are directed towards the development of methods for artemisinin production via heterologous expression systems. We used in this study agrobacterial transformation to transfer the genetic module of the artemisinin biosynthesis pathway into plants and then analyzed its transcription in a heterologous host. Tobacco plants were transformed with the artemisinin biosynthesis genes encoding amorpha-4,11-diene synthase, artemisin-aldehyde All(13) reductase, amorpha-4,11-diene monooxygenase, cytochrome P450 reductase from A. annua and yeast 3-hydroxy-3-methylglutaryl-coenzyme A reductase cloned in the pArtemC vector; farnesyl diphosphate synthase and aldehyde dehydrogenase were used to transform the plants as parts of vector p2356. As a result of transformation with the pArtemC and p2356 vectors, in twos transgenic lines with all target genes were obtained. Five genes of artemisinin biosynthesis and two genes of biosynthesis of its precursors were successfully transferred into the genome of transgenic tobacco lines as a result of the co-transformation with abovementioned vectors. Thus, the entire artemisinin biosynthesis pathway was first reconstructed in heterologous plants: the transcription of the artemisinin biosynthesis genes in the tobacco plants was shown via RT-PCR. The obtained results will be used in further research on expression systems for the production of artemisinin and other non-protein substances in heterologous host plants. artemisinin, malaria, metabolic engineering, tobacco, transgenic plants This work was supported by a Grant from the Russian Science Foundation no. 19-14-00190.

Arbuscular mycorrhiza increase artemisinin accumulation in Artemisia annua by higher expression of key biosynthesis genes via enhanced jasmonic acid levels

Mycorrhiza ◽  
2014 ◽  
Vol 25 (5) ◽  
pp. 345-357 ◽  
Author(s):  
Shantanu Mandal ◽  
Shivangi Upadhyay ◽  
Saima Wajid ◽  
Mauji Ram ◽  
Dharam Chand Jain ◽  
...  
Keyword(s):  
Jasmonic Acid ◽  
Arbuscular Mycorrhiza ◽  
Artemisia Annua

Localization of enzymes of artemisinin biosynthesis to the apical cells of glandular secretory trichomes of Artemisia annua L.

2009 ◽  
Vol 70 (9) ◽  
pp. 1123-1128 ◽  
Author(s):  
Mikael E. Olsson ◽  
Linda M. Olofsson ◽  
Ann-Louise Lindahl ◽  
Anneli Lundgren ◽  
Maria Brodelius ◽  
...  
Keyword(s):  
Artemisia Annua ◽  
Artemisia Annua L ◽  
Apical Cells ◽  
Artemisinin Biosynthesis

Transplanting and harvesting effect on artemisinin biosynthesis and herb yield in Artemisia annua L.

2015 ◽  
Vol 89 (4) ◽  
pp. 751-757 ◽  
Author(s):  
Shilpi Paul ◽  
Suman Preet Singh Khanuja ◽  
Madan Mohan Gupta
Keyword(s):  
Artemisia Annua ◽  
Artemisia Annua L ◽  
Artemisinin Biosynthesis ◽  
Herb Yield
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