scholarly journals Remodeling of Leaf Cellular Glycerolipid Composition under Drought and Re-hydration Conditions in Grasses from the Lolium-Festuca Complex

2016 ◽  
Vol 7 ◽  
Author(s):  
Dawid Perlikowski ◽  
Sylwia Kierszniowska ◽  
Aneta Sawikowska ◽  
Paweł Krajewski ◽  
Marcin Rapacz ◽  
...  
Keyword(s):  
Glycerolipid Composition

scholarly journals High light induces species specific changes in the membrane lipid composition of Chlorella

2020 ◽  
Vol 477 (13) ◽  
pp. 2543-2559
Author(s):  
Janka Widzgowski ◽  
Alexander Vogel ◽  
Lena Altrogge ◽  
Julia Pfaff ◽  
Heiko Schoof ◽  
...  
Keyword(s):  
Cell Membranes ◽  
De Novo ◽  
Algal Cell ◽  
Isotopic Labeling ◽  
Biochemical Properties ◽  
Membrane Lipid ◽  
Light Conditions ◽  
Hydrophobic Barrier ◽  
Glycerolipid Composition ◽  
Species Specific

Algae have evolved several mechanisms to adjust to changing environmental conditions. To separate from their surroundings, algal cell membranes form a hydrophobic barrier that is critical for life. Thus, it is important to maintain or adjust the physical and biochemical properties of cell membranes which are exposed to environmental factors. Especially glycerolipids of thylakoid membranes, the site of photosynthesis and photoprotection within chloroplasts, are affected by different light conditions. Since little is known about membrane lipid remodeling upon different light treatments, we examined light induced alterations in the glycerolipid composition of the two Chlorella species, C. vulgaris and C. sorokiniana, which differ strongly in their ability to cope with different light intensities. Lipidomic analysis and isotopic labeling experiments revealed differences in the composition of their galactolipid species, although both species likely utilize galactolipid precursors originated from the endoplasmic reticulum. However, in silico research of de novo sequenced genomes and ortholog mapping of proteins putatively involved in lipid metabolism showed largely conserved lipid biosynthesis pathways suggesting species specific lipid remodeling mechanisms, which possibly have an impact on the response to different light conditions.

Effect of alleles governing 16:0 concentration on glycerolipid composition in developing soybeans

2001 ◽  
Vol 78 (4) ◽  
pp. 329-334 ◽  
Author(s):  
R. F. Wilson ◽  
T. C. Marquardt ◽  
W. P. Novitzky ◽  
J. W. Burton ◽  
J. R. Wilcox ◽  
...  
Keyword(s):  
Glycerolipid Composition

scholarly journals Multiple reaction monitoring mass spectrometry is a powerful tool to study glycerolipid composition in plants with different level of desaturase activity

2013 ◽  
Vol 8 (5) ◽  
pp. e24118 ◽  
Author(s):  
Nabila Djafi ◽  
Lydie Humbert ◽  
Dominique Rainteau ◽  
Catherine Cantrel ◽  
Alain Zachowski ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Desaturase Activity ◽  
Multiple Reaction Monitoring ◽  
Reaction Monitoring ◽  
Glycerolipid Composition

Characterization of the glycerolipid composition of a high-palmitoleic acid sunflower mutant

2007 ◽  
Vol 109 (6) ◽  
pp. 591-599 ◽  
Author(s):  
Joaquín J. Salas ◽  
Antonio J. Moreno-Pérez ◽  
Enrique Martínez-Force ◽  
Rafael Garcés
Keyword(s):  
Palmitoleic Acid ◽  
Sunflower Mutant ◽  
Glycerolipid Composition

Determination of glycerolipid composition of rice and maize tissues using solid-phase extraction

2000 ◽  
Vol 28 (6) ◽  
pp. 586-589 ◽  
Author(s):  
I. Rizov ◽  
A. Doulis
Keyword(s):  
Solid Phase Extraction ◽  
Membrane Lipids ◽  
Solid Phase ◽  
Phase Extraction ◽  
Rapid Separation ◽  
Rice Roots ◽  
Maize Leaves ◽  
Glycerolipid Composition

Currently available techniques for the separation and characterization of different glycerolipids are complicated and/or time consuming. By modulating the stationary phase in a solid-phase extraction (SPE) manifold, efficient and rapid separation of plant membrane lipids was achieved. The glycerolipids from rice and maize tissues were separated into seven classes (monogalactosyldiacylglycerol, digalactosyldiacylglycerol, phosphatidylethanolamine, phosphatidylcholine, sulphoquinovosyldiacylglycerol, phosphatidylinositol and phosphatidylglycerol). The pigments present in the rice and maize leaves and rice stems were successfully removed from the total lipid extracts. Pigment-free plant tissue (rice roots) was also analysed. The fatty acid profile of each lipid class isolated by SPE agreed well with those obtained by other separation techniques. The recovery of glycerolipids was at least 87°.

Leaf lipids of Ribes nigrum: a plant containing 16:3, α-18:3, γ-18:3 and 18:4 fatty acids

2000 ◽  
Vol 28 (6) ◽  
pp. 583-586 ◽  
Author(s):  
G. Dobson
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Linolenic Acid ◽  
Stearidonic Acid ◽  
Ribes Nigrum ◽  
Total Fatty Acid Composition ◽  
Minor Contribution ◽  
Glycerolipid Composition ◽  
A Minor ◽  
Hexadecatrienoic Acid

The glycerolipid composition of Ribes nigrum (blackcurrant) leaves was determined. The total fatty acid composition was unusual in that α-linolenic acid (α-18:3) occurred together with cis-7,10,13-hexadecatrienoic acid (16:3) and lower amounts of stearidonic acid (18:4) and γ-linolenic acid (γ-18:3). Monogalactosyldiacylglycerol contained the highest proportion of 16:3 with less in digalactosyldiacylglycerol. γ-18:3 and 18:4 were present in all lipids and 18:4 was always greater than γ-18:3. The highest percentages of γ-18:3 and 18:4 were in phosphatidylcholine, but phosphatidylglycerol was particularly low in these acids. In summary, the lipid composition was largely typical of 16:3 plants but there was a minor contribution typical of 18:4 plants. The possibility of three pathways for glycolipid biosynthesis is discussed.

scholarly journals Characterization of the Glycerolipid Composition and Biosynthetic Capacity of Pea Root Plastids

1997 ◽  
Vol 113 (2) ◽  
pp. 549-557 ◽  
Author(s):  
L. Xue ◽  
L. M. McCune ◽  
K. F. Kleppinger-Sparace ◽  
M. J. Brown ◽  
M. K. Pomeroy ◽  
...  
Keyword(s):  
Pea Root ◽  
Glycerolipid Composition

scholarly journals Glycerolipid Composition of the Red Macroalga Agarophyton Chilensis and Comparison to the Closely Related Agarophyton Vermiculophyllum Producing Different Types of Eicosanoids

Marine Drugs ◽  
2019 ◽  
Vol 17 (2) ◽  
pp. 96 ◽  
Author(s):  
Masaki Honda ◽  
Takashi Ishimaru ◽  
Yutaka Itabashi ◽  
Mikhail Vyssotski
Keyword(s):  
Fatty Acids ◽  
Molecular Species ◽  
Reversed Phase ◽  
Total Lipid Extract ◽  
Chiral Phase ◽  
Substrate Composition ◽  
Red Macroalga ◽  
The Difference ◽  
Glycerolipid Composition ◽  
Hydroxyeicosatetraenoic Acids

The red macroalga Agarophyton chilensis is a well-known producer of eicosanoids such as hydroxyeicosatetraenoic acids, but the alga produces almost no prostaglandins, unlike the closely related A. vermiculophyllum. This indicates that the related two algae would have different enzyme systems or substrate composition. To carry out more in-depth discussions on the metabolic pathway of eicosanoids between the two algae, we investigated the characteristics of glycerolipids, which are the substrates of eicosanoids production, of A. chilensis and compared them to the reported values of A. vermiculophyllum. In A. chilensis, monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), sulfoquinovosyldiacylglycerol (SQDG), and phosphatidylcholine (PC) were the major lipid classes and accounted for 44.4% of the total lipid extract. The predominant fatty acids were arachidonic acid (20:4n-6), an eicosanoids precursor, and palmitic acid (16:0). The 20:4n-6 content was extremely high in MGDG and PC (>70%), and the 16:0 content was extremely high in DGDG and SQDG (>40%). A chiral-phase HPLC analysis showed that fatty acids were esterified at the sn-1 and sn-2 positions of those lipids. The glycerolipid molecular species were determined by reversed-phase HPLC–ESI–MS analysis. The main glycerolipid molecular species were 20:4n-6/20:4n-6 (sn-1/sn-2) for MGDG (63.8%) and PC (48.2%), 20:4n-6/16:0 for DGDG (71.1%) and SQDG (29.4%). These lipid characteristics of A. chilensis were almost the same as those of A. vermiculophyllum. Hence, the differences of the eicosanoids producing ability between the two algae would not be due to the difference of substrate composition but the difference of enzyme system.

scholarly journals Some studies on the composition and surface properties of oil bodies from the seed cotyledons of safflower (Carthamus tinctorius) and linseed (Linum ustatissimum)

1980 ◽  
Vol 190 (3) ◽  
pp. 551-561 ◽  
Author(s):  
C R Slack ◽  
W S Bertaud ◽  
B D Shaw ◽  
R Holland ◽  
J Browse ◽  
...  
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Electron Microscopic Examination ◽  
Carthamus Tinctorius ◽  
Oil Body ◽  
Oil Bodies ◽  
Electron Microscopic ◽  
Intact Cells ◽  
Glycerolipid Composition ◽  
Induced Aggregation

1. The average oil-body diameter in intact cells of developing linseed (Linum usitatissimum) and safflower (Carthamus tinctorius) cotyledons was similar (about 1.4 micrometer), and there was little change in size after oil bodies were isolated and repeatedly washed. 2. The glycerolipid composition of washed oil bodies from both developing and mature cotyledons of the two species was similar; oil bodies from ten different batches of cotyledons contained 4.3 +/- 0.16 mumol of 3-sn-phosphatidylcholine and 25.2 +/- 1.7 mumol of diacylglycerol per 1000 mumol of triacylglycerol. During four successive washings of a once-washed oil-body preparation, the proportion of diacylglycerol to triacylglycerol remained constant and that of 3-sn-phosphatidylcholine to triacylglycerol decreased by only 20%. 3. The protein content of thrice-washed oil bodies from the two species was similar, about 2.4% of the weight of glycerolipids, and appeared to be independent of the stage of cotyledon maturity. Sodium dodecyl sulphate/polyacrylamide-gel electrophoresis indicated that the protein of purified oil bodies from the two species consisted mainly of only four polypeptides and that two of the polypeptides from each species had apparent mol.wts. of 17500 and 15500. Similar patterns of polypeptides were obtained after the hydrolysis of the 15500-mol.wt. polypeptides from linseed and safflower oil bodies by Staphylococcus aureus V8 proteinase, whereas the proteolysis of the 17500-mol.wt. polypeptides from the two species produced different patterns of polypeptides. 4. The 3-sn-phosphatidylcholine in oil-body preparations was hydrolysed about 85% by bee-venom phospholipase A2 without any apparent coalescence of the oil bodies. Incubation with lipase from Rhizopus arrhizus caused rapid coalescence of the oil bodies, and this lipase appeared to initially hydrolyse diacylglycerols in preference to triacylglycerol. 5. Oil bodies from both species were almost completely dispersed in suspensions of pH between 7.1 and 8.3, but formed large aggregates at pH values between 6.7 and 3.9; pH-induced aggregation caused no coalescence. Aggregates formed under acidic conditions were dispersed by re-adjusting the pH of suspensions to 8.3. 6. A freeze-etch electron-microscopic examination of isolated oil bodies indicated that these organelles were bounded by some form of membrane with a particle-free outer surface.

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