Characterization of the glycerolipid composition of a high-palmitoleic acid sunflower mutant

2007 ◽  
Vol 109 (6) ◽  
pp. 591-599 ◽  
Author(s):  
Joaquín J. Salas ◽  
Antonio J. Moreno-Pérez ◽  
Enrique Martínez-Force ◽  
Rafael Garcés
Keyword(s):  
Palmitoleic Acid ◽  
Sunflower Mutant ◽  
Glycerolipid Composition

Determination of glycerolipid composition of rice and maize tissues using solid-phase extraction

2000 ◽  
Vol 28 (6) ◽  
pp. 586-589 ◽  
Author(s):  
I. Rizov ◽  
A. Doulis
Keyword(s):  
Solid Phase Extraction ◽  
Membrane Lipids ◽  
Solid Phase ◽  
Phase Extraction ◽  
Rapid Separation ◽  
Rice Roots ◽  
Maize Leaves ◽  
Glycerolipid Composition

Currently available techniques for the separation and characterization of different glycerolipids are complicated and/or time consuming. By modulating the stationary phase in a solid-phase extraction (SPE) manifold, efficient and rapid separation of plant membrane lipids was achieved. The glycerolipids from rice and maize tissues were separated into seven classes (monogalactosyldiacylglycerol, digalactosyldiacylglycerol, phosphatidylethanolamine, phosphatidylcholine, sulphoquinovosyldiacylglycerol, phosphatidylinositol and phosphatidylglycerol). The pigments present in the rice and maize leaves and rice stems were successfully removed from the total lipid extracts. Pigment-free plant tissue (rice roots) was also analysed. The fatty acid profile of each lipid class isolated by SPE agreed well with those obtained by other separation techniques. The recovery of glycerolipids was at least 87°.

scholarly journals Characterization of the Glycerolipid Composition and Biosynthetic Capacity of Pea Root Plastids

1997 ◽  
Vol 113 (2) ◽  
pp. 549-557 ◽  
Author(s):  
L. Xue ◽  
L. M. McCune ◽  
K. F. Kleppinger-Sparace ◽  
M. J. Brown ◽  
M. K. Pomeroy ◽  
...  
Keyword(s):  
Pea Root ◽  
Glycerolipid Composition

Lipid characterization of a wrinkled sunflower mutant

2008 ◽  
Vol 69 (3) ◽  
pp. 684-691 ◽  
Author(s):  
Mónica Venegas-Calerón ◽  
Enrique Martínez-Force ◽  
Rafael Garcés
Keyword(s):  
Sunflower Mutant ◽  
Lipid Characterization

Lipid Characterization of a High-Stearic Sunflower Mutant Displaying a Seed Stearic Acid Gradient

2006 ◽  
Vol 54 (10) ◽  
pp. 3612-3616 ◽  
Author(s):  
Valle Fernandez-Moya ◽  
Enrique Martínez-Force ◽  
Rafael Garcés
Keyword(s):  
Stearic Acid ◽  
Sunflower Mutant ◽  
Lipid Characterization

scholarly journals On the Trail of Tetu1: Genome-Wide Discovery of CACTA Transposable Elements in Sunflower Genome

2020 ◽  
Vol 21 (6) ◽  
pp. 2021
Author(s):  
Maria Ventimiglia ◽  
Claudio Pugliesi ◽  
Alberto Vangelisti ◽  
Gabriele Usai ◽  
Tommaso Giordani ◽  
...  
Keyword(s):  
Transposable Elements ◽  
Coding Region ◽  
Gene Encoding ◽  
Bioinformatics Analyses ◽  
Repeat Sequences ◽  
Genome Wide ◽  
Sunflower Mutant ◽  
Sunflower Genome ◽  
Identification And Characterization

Much has been said about sunflower (Helianthus annuus L.) retrotransposons, representing the majority of the sunflower’s repetitive component. By contrast, class II transposons remained poorly described within this species, as they present low sequence conservation and are mostly lacking coding domains, making the identification and characterization of these transposable elements difficult. The transposable element Tetu1, is a non-autonomous CACTA-like element that has been detected in the coding region of a CYCLOIDEA (CYC) gene of a sunflower mutant, tubular ray flower (turf). Based on our knowledge of Tetu1, the publicly available genome of sunflower was fully scanned. A combination of bioinformatics analyses led to the discovery of 707 putative CACTA sequences: 84 elements with complete ends and 623 truncated elements. A detailed characterization of the identified elements allowed further classification into three subgroups of 347 elements on the base of their terminal repeat sequences. Only 39 encode a protein similar to known transposases (TPase), with 10 TPase sequences showing signals of activation. Finally, an analysis of the proximity of CACTA transposons to sunflower genes showed that the majority of CACTA elements are close to the nearest gene, whereas a relevant fraction resides within gene-encoding sequences, likely interfering with sunflower genome functionality and organization.

scholarly journals Fatty acid characterization of indigenous cyanobacterial strains isolated from five hot springs in indonesia

2018 ◽  
Vol 67 ◽  
pp. 02021 ◽  
Author(s):  
Nining Betawati Prihantini ◽  
Sri Handayani ◽  
Wellyzar Sjamsuridzal ◽  
Akira Yokota ◽  
Nasruddin
Keyword(s):  
Fatty Acids ◽  
Extraction Method ◽  
Palmitoleic Acid ◽  
Hot Springs ◽  
Unsaturated Fatty Acids ◽  
Raw Materials ◽  
Saturated Fatty Acids ◽  
Microbial Identification ◽  
Almost All

Cyanobacteria have been known to produce lipids that are potential for biodiesel. Cyanobacteria isolated from Indonesia are called Indonesia indigenous cyanobacteria. This study was conducted to determine the characterization of fatty acids contained in cyanobacteria originating from Indonesia which were isolated from 5 hot springs in Indonesia. For some 29 strains of cyanobacteria consisting of 8 genera have performed the analysis of fatty acids (FA) by extraction method via protocol in SHERLOCK Microbial Identification (Midi) System version 4.0, 2001 MIDI, Inc. The resulting data is as follows. All strains of 8 genera (Synechococcus, Merismopedia, Thermosynechococcus, Stanieria, Leptolyngbia, Westiellopsis, Mastigocladus, and Nostoc) have saturated fatty acids (SFA) and unsaturated fatty acids (MUFA and PUFA). The content of saturated fatty acids ranged from 27.77 to 50.56%, while the content of unsaturated fatty acids ranged from 7.58 to 63.31%. All strains have SFA Palmitic acid (16:00) which ranges from 23.23 to 42.64%. Meanwhile, unsaturated fatty acids Palmitoleic acid (16:1 wc7) are owned by almost all strains except Westiellopsis which range from 1.75 to 51.78%. Content of unsaturated fatty acids Oleic acid (18: w9c) ranges from 1.43 to 35.78% mainly in Leptolyngbia, Westiellopsis, and Mastigocladus. All strains have MUFA ranging from 7.58 to 63.31%, whereas PUFA is only owned by filamentous strains (Leptolyngbia, Westiellposis, Mastigocladus, and Nostoc). From the results of the research can be seen that 29 strains of cyanobacteria of 8 genera have potential fatty acids as raw materials of biodiesel under certain conditions.

Biochemical characterization of a high-palmitoleic acid Helianthus annuus mutant

2004 ◽  
Vol 42 (5) ◽  
pp. 373-381 ◽  
Author(s):  
Joaquín J. Salas ◽  
Enrique Martínez-Force ◽  
Rafael Garcés
Keyword(s):  
Helianthus Annuus ◽  
Palmitoleic Acid ◽  
Biochemical Characterization

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

AEM analysis of crystallization in Ti-based amorphous alloys

1983 ◽  
Vol 41 ◽  
pp. 270-271
Author(s):  
A.R. Pelton ◽  
A.F. Marshall ◽  
Y.S. Lee
Keyword(s):  
Magnetic Properties ◽  
Amorphous Alloys ◽  
Amorphous Materials ◽  
Isothermal Annealing ◽  
Amorphous Matrix ◽  
Nucleation And Growth ◽  
Current Interest ◽  
Amorphous Films ◽  
Electrical And Magnetic Properties

Amorphous materials are of current interest due to their desirable mechanical, electrical and magnetic properties. Furthermore, crystallizing amorphous alloys provides an avenue for discerning sequential and competitive phases thus allowing access to otherwise inaccessible crystalline structures. Previous studies have shown the benefits of using AEM to determine crystal structures and compositions of partially crystallized alloys. The present paper will discuss the AEM characterization of crystallized Cu-Ti and Ni-Ti amorphous films.Cu60Ti40: The amorphous alloy Cu60Ti40, when continuously heated, forms a simple intermediate, macrocrystalline phase which then transforms to the ordered, equilibrium Cu3Ti2 phase. However, contrary to what one would expect from kinetic considerations, isothermal annealing below the isochronal crystallization temperature results in direct nucleation and growth of Cu3Ti2 from the amorphous matrix.

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