scholarly journals Functional Characterization of a Gene in Sedum alfredii Hance Resembling Rubber Elongation Factor Endowed with Functions Associated with Cadmium Tolerance

2016 ◽  
Vol 7 ◽  
Author(s):  
Mingying Liu ◽  
Wenming Qiu ◽  
Xuelian He ◽  
Liu Zheng ◽  
Xixi Song ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Elongation Factor ◽  
Cadmium Tolerance ◽  
Sedum Alfredii ◽  
Sedum Alfredii Hance

Functional analysis of a stable transcription arrest site in the first intron of the murine adenosine deaminase gene

1993 ◽  
Vol 13 (5) ◽  
pp. 2718-2729
Author(s):  
S F Kash ◽  
J W Innis ◽  
A U Jackson ◽  
R E Kellems
Keyword(s):  
Rna Polymerase Ii ◽  
Adenosine Deaminase ◽  
Functional Characterization ◽  
Gel Filtration ◽  
Point Mutations ◽  
Elongation Factor ◽  
Dependent Manner ◽  
First Intron ◽  
Intron 1

Transcription arrest plays a role in regulating the expression of a number of genes, including the murine adenosine deaminase (ADA) gene. We have previously identified two prominent arrest sites at the 5' end of the ADA gene: one in the first exon and one in the first intron (J. W. Innis and R. E. Kellems, Mol. Cell. Biol. 11:5398-5409, 1991). Here we report the functional characterization of the intron 1 arrest site, located 137 to 145 nucleotides downstream of the cap site. We have determined, using gel filtration, that the intron 1 arrest site is a stable RNA polymerase II pause site and that the transcription elongation factor SII promotes read-through at this site. Additionally, the sequence determinants for the pause are located within a 37-bp fragment encompassing this site (+123 to +158) and can direct transcription arrest in an orientation-dependent manner in the context of the ADA and adenovirus major late promoters. Specific point mutations in this region increase or decrease the relative pausing efficiency. We also show that the sequence determinants for transcription arrest can function when placed an additional 104 bp downstream of their natural position.

Identification and Characterization of Four Nicotianamine Synthase Genes in Sedum alfredii Hance

2018 ◽  
Vol 12 (6) ◽  
pp. 551-559 ◽  
Author(s):  
Shaoning Chen ◽  
Zulfiqar Ali Sahito ◽  
Min Zhang ◽  
Ying Feng ◽  
Qianying Yang ◽  
...  
Keyword(s):  
Sedum Alfredii ◽  
Sedum Alfredii Hance ◽  
Nicotianamine Synthase ◽  
Identification And Characterization

Cadmium tolerance and hyperaccumulation in a new Zn-hyperaccumulating plant species (Sedum alfredii Hance)

2004 ◽  
Vol 259 (1/2) ◽  
pp. 181-189 ◽  
Author(s):  
X.E. Yang ◽  
X.X. Long ◽  
H.B. Ye ◽  
Z.L. He ◽  
D.V. Calvert ◽  
...  
Keyword(s):  
Plant Species ◽  
Cadmium Tolerance ◽  
Sedum Alfredii ◽  
Sedum Alfredii Hance ◽  
Hyperaccumulating Plant

scholarly journals Identification, Expression Analysis of the Hsf Family, and Characterization of Class A4 in Sedum Alfredii Hance under Cadmium Stress

2018 ◽  
Vol 19 (4) ◽  
pp. 1216 ◽  
Author(s):  
Shuang-Shuang Chen ◽  
Jing Jiang ◽  
Xiao-Jiao Han ◽  
Yun-Xing Zhang ◽  
Ren-Ying Zhuo
Keyword(s):  
Expression Analysis ◽  
Cadmium Stress ◽  
Sedum Alfredii ◽  
Sedum Alfredii Hance

scholarly journals Functional analysis of a stable transcription arrest site in the first intron of the murine adenosine deaminase gene.

1993 ◽  
Vol 13 (5) ◽  
pp. 2718-2729 ◽  
Author(s):  
S F Kash ◽  
J W Innis ◽  
A U Jackson ◽  
R E Kellems
Keyword(s):  
Rna Polymerase Ii ◽  
Adenosine Deaminase ◽  
Functional Characterization ◽  
Gel Filtration ◽  
Point Mutations ◽  
Elongation Factor ◽  
Dependent Manner ◽  
First Intron ◽  
Intron 1

Transcription arrest plays a role in regulating the expression of a number of genes, including the murine adenosine deaminase (ADA) gene. We have previously identified two prominent arrest sites at the 5' end of the ADA gene: one in the first exon and one in the first intron (J. W. Innis and R. E. Kellems, Mol. Cell. Biol. 11:5398-5409, 1991). Here we report the functional characterization of the intron 1 arrest site, located 137 to 145 nucleotides downstream of the cap site. We have determined, using gel filtration, that the intron 1 arrest site is a stable RNA polymerase II pause site and that the transcription elongation factor SII promotes read-through at this site. Additionally, the sequence determinants for the pause are located within a 37-bp fragment encompassing this site (+123 to +158) and can direct transcription arrest in an orientation-dependent manner in the context of the ADA and adenovirus major late promoters. Specific point mutations in this region increase or decrease the relative pausing efficiency. We also show that the sequence determinants for transcription arrest can function when placed an additional 104 bp downstream of their natural position.

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