Functional characterization of the promoter region of the chicken elongation factor-2 gene

Gene ◽  
2007 ◽  
Vol 386 (1-2) ◽  
pp. 183-190 ◽  
Author(s):  
Eun Jin Lim ◽  
Choong Won Kim
Keyword(s):  
Promoter Region ◽  
Functional Characterization ◽  
Elongation Factor ◽  
Elongation Factor 2

scholarly journals Saccharomyces cerevisiae elongation factor 2. Genetic cloning, characterization of expression, and G-domain modeling.

1992 ◽  
Vol 267 (2) ◽  
pp. 1190-1197 ◽  
Author(s):  
J P Perentesis ◽  
L D Phan ◽  
W B Gleason ◽  
D C LaPorte ◽  
D M Livingston ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Elongation Factor ◽  
Domain Modeling ◽  
Elongation Factor 2

Functional analysis of a stable transcription arrest site in the first intron of the murine adenosine deaminase gene

1993 ◽  
Vol 13 (5) ◽  
pp. 2718-2729
Author(s):  
S F Kash ◽  
J W Innis ◽  
A U Jackson ◽  
R E Kellems
Keyword(s):  
Rna Polymerase Ii ◽  
Adenosine Deaminase ◽  
Functional Characterization ◽  
Gel Filtration ◽  
Point Mutations ◽  
Elongation Factor ◽  
Dependent Manner ◽  
First Intron ◽  
Intron 1

Transcription arrest plays a role in regulating the expression of a number of genes, including the murine adenosine deaminase (ADA) gene. We have previously identified two prominent arrest sites at the 5' end of the ADA gene: one in the first exon and one in the first intron (J. W. Innis and R. E. Kellems, Mol. Cell. Biol. 11:5398-5409, 1991). Here we report the functional characterization of the intron 1 arrest site, located 137 to 145 nucleotides downstream of the cap site. We have determined, using gel filtration, that the intron 1 arrest site is a stable RNA polymerase II pause site and that the transcription elongation factor SII promotes read-through at this site. Additionally, the sequence determinants for the pause are located within a 37-bp fragment encompassing this site (+123 to +158) and can direct transcription arrest in an orientation-dependent manner in the context of the ADA and adenovirus major late promoters. Specific point mutations in this region increase or decrease the relative pausing efficiency. We also show that the sequence determinants for transcription arrest can function when placed an additional 104 bp downstream of their natural position.

scholarly journals Functional characterization of Lilium lancifolium cold-responsive Zinc Finger Homeodomain (ZFHD) gene in abscisic acid and osmotic stress tolerance

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11508
Author(s):  
Yubing Yong ◽  
Yue Zhang ◽  
Yingmin Lyu
Keyword(s):  
Abscisic Acid ◽  
Transgenic Plants ◽  
Zinc Finger ◽  
Promoter Region ◽  
Salt Water ◽  
Functional Characterization ◽  
Regulatory Elements ◽  
Response Analysis ◽  
Lilium Lancifolium

Background. We have previously performed an analysis of the cold-responsive transcriptome in the mature leaves of tiger lily (Lilium lancifolium) by gene co-expression network identification. The results has revealed that a ZFHD gene, notated as encoding zinc finger homeodomain protein, may play an essential regulating role in tiger lily response to cold stress. Methods. A further investigation of the ZFHD gene (termed as LlZFHD4) responding to osmotic stresses, including cold, salt, water stresses, and abscisic acid (ABA) was performed in this study. Based on the transcriptome sequences, the coding region and 5′ promoter region of LlZFHD4 were cloned from mature tiger lily leaves. Stress response analysis was performed under continuous 4 °C, NaCl, PEG, and ABA treatments. Functional characterization of LlZFHD4 was conducted in transgenic Arabidopsis, tobacco, and yeast. Results. LlZFHD4 encodes a nuclear-localized protein consisting of 180 amino acids. The N-terminal region of LlZFHD4 has transcriptional activation activity in yeast. The 4 °C, NaCl, PEG, and ABA treatments induced the expression of LlZFHD4. Several stress- or hormone-responsive cis-acting regulatory elements (T-Box, BoxI. and ARF) and binding sites of transcription factors (MYC, DRE and W-box) were found in the core promoter region (789 bp) of LlZFHD4. Also, the GUS gene driven by LlZFHD4 promoter was up-regulated by cold, NaCl, water stresses, and ABA in Arabidopsis. Overexpression of LlZFHD4 improved cold and drought tolerance in transgenic Arabidopsis; higher survival rate and better osmotic adjustment capacity were observed in LlZFHD4 transgenic plants compared to wild type (WT) plants under 4 °C and PEG conditions. However, LlZFHD4 transgenic plants were less tolerant to salinity and more hypersensitive to ABA compared to WT plants. The transcript levels of stress- and ABA-responsive genes were much more up-regulated in LlZFHD4 transgenic Arabidopsis than WT. These results indicate LlZFHD4 is involved in ABA signaling pathway and plays a crucial role in regulating the response of tiger lily to cold, salt and water stresses.

scholarly journals Functional Characterization of Variants inMC4RGene Promoter Region Found in Obese Children

2014 ◽  
Vol 99 (5) ◽  
pp. E931-E935 ◽  
Author(s):  
Karen Mei Ling Tan ◽  
Shu Qin Delicia Ooi ◽  
Siong Gim Ong ◽  
Charmaine Shuyi Kwan ◽  
Raymond Ming En Chan ◽  
...  
Keyword(s):  
Promoter Region ◽  
Functional Characterization ◽  
Obese Children

Structural and functional characterization of the promoter region of the mouse c-Ki-ras gene

1987 ◽  
Vol 7 (7) ◽  
pp. 2592-2596
Author(s):  
E K Hoffman ◽  
S P Trusko ◽  
N Freeman ◽  
D L George
Keyword(s):  
Promoter Region ◽  
Promoter Activity ◽  
Functional Characterization ◽  
Genetic Material ◽  
Bidirectional Promoter ◽  
Adrenocortical Tumor ◽  
Ras Gene ◽  
Untranslated Exon ◽  
Size Heterogeneity

We isolated and characterized the 5' region of the mouse c-Ki-ras gene, including a 5' untranslated exon (exon 0). These studies used genetic material from Y1 mouse adrenocortical tumor cells in which the c-Ki-ras gene is amplified and overexpressed. Our data demonstrate that transcription initiates at multiple sites, predicting size heterogeneity at the 5' ends of the c-Ki-ras mRNAs. Using transient expression assays, we identified a genomic fragment within the 5' region which exhibits bidirectional promoter activity.

scholarly journals Purification and characterization of native human elongation factor 2

2019 ◽  
Vol 158 ◽  
pp. 15-19
Author(s):  
Rasmus Kock Flygaard ◽  
Beatrice Malacrida ◽  
Patrick Kiely ◽  
Lasse Bohl Jenner
Keyword(s):  
Elongation Factor ◽  
Purification And Characterization ◽  
Elongation Factor 2 ◽  
Human Elongation Factor

Molecular cloning and characterization of a Cryptosporidium parvum elongation factor-2 gene

1995 ◽  
Vol 71 (1) ◽  
pp. 143-147 ◽  
Author(s):  
Douglas E. Jones ◽  
Tu D. Tu ◽  
Sameer Mathur ◽  
Raymond W. Sweeney ◽  
Douglas P. Clark
Keyword(s):  
Molecular Cloning ◽  
Cryptosporidium Parvum ◽  
Elongation Factor ◽  
Elongation Factor 2

scholarly journals Functional Characterization of 4 Polymorphisms in Promoter Region of Hepatic Lipase Gene

2000 ◽  
Vol 20 (5) ◽  
pp. 1335-1339 ◽  
Author(s):  
Ferdinand M. van ′t Hooft ◽  
Björn Lundahl ◽  
Francesca Ragogna ◽  
Fredrik Karpe ◽  
Gunilla Olivecrona ◽  
...  
Keyword(s):  
Promoter Region ◽  
Hepatic Lipase ◽  
Functional Characterization ◽  
Lipase Gene ◽  
Hepatic Lipase Gene

scholarly journals Identification and functional characterization of the promoter region of the human organic anion transporting polypeptide gene

Hepatology ◽  
1997 ◽  
Vol 26 (4) ◽  
pp. 991-997 ◽  
Author(s):  
G Kullak-Ublick ◽  
U Beuers ◽  
C Fahney ◽  
B Hagenbuch ◽  
P J Meier ◽  
...  
Keyword(s):  
Promoter Region ◽  
Organic Anion ◽  
Functional Characterization ◽  
Organic Anion Transporting Polypeptide
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