scholarly journals Nanonets Derived from Turnip Mosaic Virus as Scaffolds for Increased Enzymatic Activity of Immobilized Candida antarctica Lipase B

2016 ◽  
Vol 7 ◽  
Author(s):  
Sol Cuenca ◽  
Carmen Mansilla ◽  
Marta Aguado ◽  
Carmen Yuste-Calvo ◽  
Flora Sánchez ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Mosaic Virus ◽  
Turnip Mosaic Virus ◽  
Candida Antarctica ◽  
Candida Antarctica Lipase B ◽  
Candida Antarctica Lipase ◽  
Lipase B ◽  
Immobilized Candida Antarctica Lipase

scholarly journals Kinetic Modeling and Optimization of Immobilized Candida antarctica Lipase B Catalysed Synthesis of Butyl-4-Methyl-3-Oxopentanoate using Response Surface Methodology

2012 ◽  
Vol 10 (1) ◽  
Author(s):  
Ganapati D. Yadav ◽  
Somnath Dattatray Shinde
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Polynomial Regression ◽  
Enzymatic Reaction ◽  
Candida Antarctica ◽  
Candida Antarctica Lipase B ◽  
Suitable Model ◽  
Candida Antarctica Lipase ◽  
Lipase B ◽  
Immobilized Candida Antarctica Lipase

Abstract Response surface methodology (RSM) was used to model and optimize the immobilized Candida antarctica lipase B catalysed synthesis of butyl-4-methyl-3-oxopentanoate. To determine optimum conditions of the transesterification, a four-factor and five-level central composite rotatable design (CCRD) was used. The factors studied were enzyme load (A), reaction temperature (B), methyl-4-methyl-3-oxopentanoate concentration (C) and n-butanol concentration (D). A quadratic polynomial regression model was used to analyze the experimental data at a 95% confidence level (p < 0.05). The results indicated that the RSM approach gave reasonable results for the optimization of the reaction parameters in the range of tested parameters. The optimal conditions for the enzymatic reaction were obtained at 0.01 mol of methyl-4-methyl-3-oxopentanoate and 0.03 mol of n-butanol using 104 mg of Novozym 435 at 55 °C and 300 rpm for 6 h. Under these conditions, the transesterification percentage was 87 %. Further, kinetic modelling of the enzymatic synthesis was illustrated. Initial rate data and progress curve data were used to arrive at a suitable model. The kinetics was found to obey the ternary complex ordered bi-bi model with inhibition by the substrate methyl-4-methyl-3-oxopentanoate. The values of kinetic parameters obtained from nonlinear regression analysis were found to be Vmax of 0.04 mol/L.min; Km(A) 0.11 mol/L; Km(B) 2 mol/L and Ki(A) 2.2 mol/L.

Production of Omega-3 Triacylglycerol Concentrates using a New Food Grade Immobilized Candida antarctica Lipase B

2010 ◽  
Vol 63 (6) ◽  
pp. 922 ◽  
Author(s):  
Jaroslav A. Kralovec ◽  
Weijie Wang ◽  
Colin J. Barrow
Keyword(s):  
Fatty Acids ◽  
Candida Antarctica ◽  
Packed Bed Reactor ◽  
Candida Antarctica Lipase B ◽  
Candida Antarctica Lipase ◽  
Omega 3 ◽  
Lipase B ◽  
Food Grade ◽  
Commercial System ◽  
Immobilized Candida Antarctica Lipase

Triacylglycerol concentrates of eicosapentaenoic and docosahexaenoic omega-3 fatty acids were synthesized either via transesterification or esterification of glycerol with the corresponding ethyl ester or free fatty acid concentrates, respectively. A newly developed food grade immobilized Candida antarctica lipase B system using an Amberlite FPX-66 hydrophobic matrix, was compared with a commercially available non-food grade commercial system, for their ability to catalyze these reactions. For either system, the transesterification required higher temperature (90°C) than esterification (70°C) to achieve maximum triacylglycerol yields. The newly developed immobilized system efficiently catalyzes the esterification of free fatty acids with glycerol and differs from the existing commercial system in that it is food grade and has a more uniform and larger particle distribution. The new system significantly improves flow in a packed bed reactor, enabling multiple reuse of the catalyst for up to 80 repeats.

Production of 6-O-l-Ascorbyl Palmitate by Immobilized Candida antarctica Lipase B

2017 ◽  
Vol 184 (4) ◽  
pp. 1168-1186 ◽  
Author(s):  
Manish G. Yadav ◽  
Monali R. Kavadia ◽  
Rajeshkumar N. Vadgama ◽  
Annamma A. Odaneth ◽  
Arvind M. Lali
Keyword(s):  
Ascorbyl Palmitate ◽  
Candida Antarctica ◽  
Candida Antarctica Lipase B ◽  
Candida Antarctica Lipase ◽  
Lipase B ◽  
Immobilized Candida Antarctica Lipase
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