On the importance of the supporting material for activity of immobilized Candida antarctica lipase B in ionic liquid/hexane and ionic liquid/supercritical carbon dioxide biphasic media

2007 ◽  
Vol 40 (1) ◽  
pp. 93-100 ◽  
Author(s):  
Pedro Lozano ◽  
Teresa De Diego ◽  
Tanja Sauer ◽  
Michel Vaultier ◽  
Said Gmouh ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Ionic Liquid ◽  
Supercritical Carbon Dioxide ◽  
Candida Antarctica ◽  
Candida Antarctica Lipase B ◽  
Candida Antarctica Lipase ◽  
Lipase B ◽  
Supporting Material ◽  
Immobilized Candida Antarctica Lipase ◽  
Supercritical Carbon

A Continuous Reactor for the (Chemo)enzymatic Dynamic Kinetic Resolution of Rac-1-Phenylethanol in Ionic Liquid/Supercritical Carbon Dioxide Biphasic Systems

2007 ◽  
Vol 5 (1) ◽  
Author(s):  
Pedro Lozano ◽  
Teresa De Diego ◽  
Said Gmouh ◽  
Michel Vaultier ◽  
Jose L Iborra
Keyword(s):  
Carbon Dioxide ◽  
Ionic Liquid ◽  
Supercritical Carbon Dioxide ◽  
Kinetic Resolution ◽  
Candida Antarctica ◽  
Candida Antarctica Lipase B ◽  
Candida Antarctica Lipase ◽  
Dynamic Kinetic Resolution ◽  
Lipase B ◽  
Biphasic Systems

Enzymatic transformations in ionic liquids (ILs)/supercritical carbon dioxide (scCO2) biphasic systems, whereby enzyme molecules are ``immobilized" in the IL phase and substrates/products are transported by the scCO2 phase, are described as a way for carrying out clean synthetic chemical processes to produce pure products. As a model system to study, the ionic liquid butyltrimethyl ammonium (bis(trifluoromethane)sulfonyl imine) ([btma][NTf2]) was assayed for Candida antarctica lipase B (CALB)-catalyzed the kinetic resolution of rac-1-phenylethanol. The suitability of this IL was established from both the activity and stability of the enzyme. A continuous dynamic kinetic resolution (DKR) process in [btma][NTf2])/scCO2 biphasic system was carried out by using simultaneously both immobilized Candida antarctica lipase B in silica gel and silica modified with benzenosulfonic acid groups (SCX) catalysts at 50 ºC and 10 MPa, providing a good yield (78 %) for (R)-1-phenylethyl propionate product with excellent enantioselectivity (ee = 92 %) in continuous operation.

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