scholarly journals Melatonin Improved Anthocyanin Accumulation by Regulating Gene Expressions and Resulted in High Reactive Oxygen Species Scavenging Capacity in Cabbage

2016 ◽  
Vol 7 ◽  
Author(s):  
Na Zhang ◽  
Qianqian Sun ◽  
Hongfei Li ◽  
Xingsheng Li ◽  
Yunyun Cao ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Anthocyanin Accumulation ◽  
Gene Expressions ◽  
Scavenging Capacity ◽  
High Reactive Oxygen Species

scholarly journals NADPH Oxidase as a Therapeutic Target for Oxalate Induced Injury in Kidneys

2013 ◽  
Vol 2013 ◽  
pp. 1-18 ◽  
Author(s):  
Sunil Joshi ◽  
Ammon B. Peck ◽  
Saeed R. Khan
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Nadph Oxidase ◽  
Tissue Injury ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Reactive Oxygen ◽  
Renal Tissue ◽  
Oxygen Species ◽  
Gene Expressions

A major role of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase family of enzymes is to catalyze the production of superoxides and other reactive oxygen species (ROS). These ROS, in turn, play a key role as messengers in cell signal transduction and cell cycling, but when they are produced in excess they can lead to oxidative stress (OS). Oxidative stress in the kidneys is now considered a major cause of renal injury and inflammation, giving rise to a variety of pathological disorders. In this review, we discuss the putative role of oxalate in producing oxidative stress via the production of reactive oxygen species by isoforms of NADPH oxidases expressed in different cellular locations of the kidneys. Most renal cells produce ROS, and recent data indicate a direct correlation between upregulated gene expressions of NADPH oxidase, ROS, and inflammation. Renal tissue expression of multiple NADPH oxidase isoforms most likely will impact the future use of different antioxidants and NADPH oxidase inhibitors to minimize OS and renal tissue injury in hyperoxaluria-induced kidney stone disease.

Synergistic Antibacterial Effect of the Combination of ɛ-Polylysine and Nisin against Enterococcus faecalis

2015 ◽  
Vol 78 (12) ◽  
pp. 2200-2206 ◽  
Author(s):  
FANG LIU ◽  
MEI LIU ◽  
LIHUI DU ◽  
DAOYING WANG ◽  
ZHIMING GENG ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Enterococcus Faecalis ◽  
Antibacterial Effect ◽  
Combined Treatment ◽  
Reactive Oxygen ◽  
Species Level ◽  
Reactive Oxygen Species Level ◽  
Oxygen Species ◽  
High Reactive Oxygen Species ◽  
Permeability Assay

This study evaluated the antibacterial effect of the combination of ɛ-polylysine (ɛ-PL) and nisin against Enterococcus faecalis strains. The combination of ɛ-PL and nisin showed synergistic antibacterial activity against three Enterococcus strains. Scanning electron microscopy and a membrane permeability assay revealed that the combined treatment with ɛ-PL and nisin synergistically damaged the cell morphology of E. faecalis strain R612Z1 cells. Both ɛ-PL and nisin can dissipate the transmembrane electric potential of E. faecalis R612Z1 cells, but these peptides did not affect the transmembrane pH gradient. The combination of ɛ-PL and nisin can produce a high reactive oxygen species level in E. faecalis R612Z1 cells. The results indicated that the uptake of ɛ-PL into cells was promoted through nisin and that the combination of ɛ-PL and nisin could produce a high reactive oxygen species level in E. faecalis R612Z1 cells, leading to cell growth inhibition.

Stable Organic Photosensitizer Nanoparticles with Absorption Peak beyond 800 Nanometers and High Reactive Oxygen Species Yield for Multimodality Phototheranostics

ACS Nano ◽  
2020 ◽  
Vol 14 (8) ◽  
pp. 9917-9928 ◽  
Author(s):  
Yingpeng Wan ◽  
Guihong Lu ◽  
Wei-Chih Wei ◽  
Yi-Hsuan Huang ◽  
Shengliang Li ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Absorption Peak ◽  
Oxygen Species ◽  
High Reactive Oxygen Species

Syringic acid triggers reactive oxygen species–mediated cytotoxicity in HepG2 cells

2019 ◽  
Vol 38 (6) ◽  
pp. 694-702 ◽  
Author(s):  
S Gheena ◽  
D Ezhilarasan
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Hepg2 Cells ◽  
Cytotoxic Effect ◽  
Reactive Oxygen ◽  
Syringic Acid ◽  
Oxygen Species ◽  
Hepg2 Cell Line ◽  
Gene Expressions

Hepatocellular carcinoma is the second most common cause of cancer death in the world and its incidence has dramatically increased worldwide in the past two decades. Syringic acid (SA) has been studied for its hepatoprotective, anti-inflammatory, immunomodulatory, free radical scavenging, and antioxidant activities. We aimed to evaluate the cytotoxic effect of SA against human hepatoma HepG2 cell line. Cytotoxicity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. HepG2 cells were treated with SA at concentration ranges of 25, 50, and 100 µM for 24 h. Reactive oxygen species (ROS) expression was investigated by dichlorofluorescein staining assay. Morphological changes of SA-treated HepG2 cells were evaluated by acridine orange (AO) and ethidium bromide (EB) dual staining. Apoptotic marker gene expressions were evaluated by qPCR. SA treatment caused significant cytotoxicity and liberation of ROS in HepG2 cells. AO and EB staining showed membrane blebbing and distortion in SA-treated cells. Apoptotic markers such as caspases 3 and 9, cytochrome c, Apaf-1, Bax, and p53 gene expressions were significantly increased upon SA treatment indicating the possibility of apoptosis induction in HepG2 cells. This treatment also caused significant downregulation of Bcl-2 gene expression. SA has a cytotoxic effect on human HepG2 cell line, and this might be a promising agent in anticancer research.

Quantification of the overall REACTIVE OXYGEN SPECIES scavenging capacity of biological fluids and tissues

2000 ◽  
Vol 28 (6) ◽  
pp. 871-879 ◽  
Author(s):  
Ron Kohen ◽  
Elangovan Vellaichamy ◽  
Jan Hrbac ◽  
Irith Gati ◽  
Oren Tirosh
Keyword(s):  
Reactive Oxygen Species ◽  
Biological Fluids ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Scavenging Capacity
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