scholarly journals Transcriptome Analysis of Brassica rapa Near-Isogenic Lines Carrying Clubroot-Resistant and –Susceptible Alleles in Response to Plasmodiophora brassicae during Early Infection

2016 ◽  
Vol 6 ◽  
Author(s):  
Jingjing Chen ◽  
Wenxing Pang ◽  
Bing Chen ◽  
Chunyu Zhang ◽  
Zhongyun Piao
Keyword(s):  
Brassica Rapa ◽  
Transcriptome Analysis ◽  
Plasmodiophora Brassicae ◽  
Early Infection ◽  
Near Isogenic Lines ◽  
Isogenic Lines

Transcriptome analysis of near-isogenic lines for glume hairiness of wheat

Gene ◽  
2020 ◽  
Vol 739 ◽  
pp. 144517
Author(s):  
Wei Luo ◽  
Jiajun Liu ◽  
Puyang Ding ◽  
Cong Li ◽  
Hang Liu ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Near Isogenic Lines ◽  
Isogenic Lines

Transcriptome analysis of leafy near‐isogenic lines provides molecular insights into floral transition in maize ( Zea mays )

2020 ◽  
Vol 139 (5) ◽  
pp. 883-891
Author(s):  
Xuemei Du ◽  
Xiaoli Wang ◽  
Jie Zhang ◽  
Sihan Zhen ◽  
Yunjun Liu ◽  
...  
Keyword(s):  
Zea Mays ◽  
Transcriptome Analysis ◽  
Floral Transition ◽  
Near Isogenic Lines ◽  
Isogenic Lines

scholarly journals Transcriptome analysis of near-isogenic lines provides molecular insights into starch biosynthesis in maize kernel

2016 ◽  
Vol 58 (8) ◽  
pp. 713-723 ◽  
Author(s):  
Yingni Xiao ◽  
Shawn Thatcher ◽  
Min Wang ◽  
Tingting Wang ◽  
Mary Beatty ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Starch Biosynthesis ◽  
Near Isogenic Lines ◽  
Maize Kernel ◽  
Isogenic Lines

scholarly journals Dissecting the Regulatory Network of Leaf Premature Senescence in Maize (Zea mays L.) Using Transcriptome Analysis of ZmELS5 Mutant

Genes ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 944
Author(s):  
Chai ◽  
Guo ◽  
Shi ◽  
Li ◽  
Tang ◽  
...  
Keyword(s):  
Zea Mays ◽  
Differentially Expressed Genes ◽  
Leaf Senescence ◽  
Transcriptome Analysis ◽  
Regulatory Network ◽  
Zea Mays L ◽  
Differentially Expressed ◽  
Premature Senescence ◽  
Near Isogenic Lines ◽  
Isogenic Lines

Leaf premature senescence largely determines maize (Zea mays L.) grain yield and quality. A natural recessive premature-senescence mutant was selected from the breeding population, and near-isogenic lines were constructed using Jing24 as the recurrent parent. In the near-isogenic lines, the dominant homozygous material was wild-type (WT), and the recessive material of early leaf senescence was the premature-senescence-type ZmELS5. To identify major genes and regulatory mechanisms involved in leaf senescence, a transcriptome analysis of the ZmELS5 and WT near-isogenic lines (NILs) was performed. A total of 8,796 differentially expressed transcripts were identified between ZmELS5 and WT, including 3,811 up-regulated and 4,985 down-regulated transcripts. By combining gene ontology, Kyoto Encyclopedia of Genes and Genomes, gene set, and transcription factor enrichment analyses, key differentially expressed genes were screened. The senescence regulatory network was predicted based on these key differentially expressed genes, which indicated that the senescence process is mainly regulated by bHLH, WRKY, and AP2/EREBP family transcription factors, leading to the accumulations of jasmonic acid and ethylene. This causes stress responses and reductions in the chlorophyll a/b-binding protein activity level. Then, decreased ATP synthase activity leads to increased photosystem II photodamage, ultimately leading to leaf senescence.

scholarly journals Primary metabolite contents are correlated with seed protein and oil traits in near-isogenic lines of soybean

2019 ◽  
Vol 7 (5) ◽  
pp. 651-659 ◽  
Author(s):  
Jie Wang ◽  
Pengfei Zhou ◽  
Xiaolei Shi ◽  
Na Yang ◽  
Long Yan ◽  
...  
Keyword(s):  
Seed Protein ◽  
Near Isogenic Lines ◽  
Primary Metabolite ◽  
Isogenic Lines

Comparative analysis of genetic background in eight near-isogenic wheat lines with different H genes conferring resistance to Hessian fly

Genome ◽  
2011 ◽  
Vol 54 (1) ◽  
pp. 81-89 ◽  
Author(s):  
S. S. Xu ◽  
C. G. Chu ◽  
M. O. Harris ◽  
C. E. Williams
Keyword(s):  
Genetic Background ◽  
Genetic Similarity ◽  
Triticum Aestivum L ◽  
Hessian Fly ◽  
Pedigree Analysis ◽  
Recurrent Parent ◽  
Linkage Drag ◽  
Near Isogenic Lines ◽  
Isogenic Lines ◽  
H Gene

Near-isogenic lines (NILs) are useful for plant genetic and genomic studies. However, the strength of conclusions from such studies depends on the similarity of the NILs’ genetic backgrounds. In this study, we investigated the genetic similarity for a set of NILs developed in the 1990s to study gene-for-gene interactions between wheat ( Triticum aestivum L.) and the Hessian fly ( Mayetiola destructor (Say)), an important pest of wheat. Each of the eight NILs carries a single H resistance gene and was created by successive backcrossing for two to six generations to susceptible T. aestivum ‘Newton’. We generated 256 target region amplification polymorphism (TRAP) markers and used them to calculate genetic similarity, expressed by the Nei and Li (NL) coefficient. Six of the NILs (H3, H5, H6, H9, H11, and H13) had the highly uniform genetic background of Newton, with NL coefficients from 0.97 to 0.99. However, genotypes with H10 or H12 were less similar to Newton, with NL coefficients of 0.86 and 0.93, respectively. Cluster analysis based on NL coefficients and pedigree analysis showed that the genetic similarity between each of the NILs and Newton was affected by both the number of backcrosses and the genetic similarity between Newton and the H gene donors. We thus generated an equation to predict the number of required backcrosses, given varying similarity of donor and recurrent parent. We also investigated whether the genetic residues of the donor parents that remained in the NILs were related to linkage drag. By using a complete set of ‘Chinese Spring’ nullisomic-tetrasomic lines, one third of the TRAP markers that showed polymorphism between the NILs and Newton were assigned to a specific chromosome. All of the assigned markers were located on chromosomes other than the chromosome carrying the H gene, suggesting that the genetic residues detected in this study were not due to linkage drag. Results will aid in the development and use of near-isogenic lines for studies of the functional genomics of wheat.

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