scholarly journals Transcriptome Analysis of Arabidopsis thaliana in Response to Plasmodiophora brassicae during Early Infection

2017 ◽  
Vol 8 ◽  
Author(s):  
Ying Zhao ◽  
Kai Bi ◽  
Zhixiao Gao ◽  
Tao Chen ◽  
Huiquan Liu ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcriptome Analysis ◽  
Plasmodiophora Brassicae ◽  
Early Infection

TRANSCRIPTOME ANALYSIS OF ARABIDOPSIS THALIANA CLUBROOTS

2006 ◽  
pp. 301-306 ◽  
Author(s):  
J. Siemens ◽  
I. Keller ◽  
J. Sarx ◽  
S. Kunz ◽  
A. Schuller ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcriptome Analysis

scholarly journals  Resistance of Arabidopsis thaliana to the obligate biotrophic parasite Plasmodiophora brassicae

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 519-522 ◽  
Author(s):  
A. Arbeiter ◽  
M. Fähling ◽  
H. Graf ◽  
M.D. Sacristán ◽  
J. Siemens
Keyword(s):  
Arabidopsis Thaliana ◽  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Plasmodiophora Brassicae ◽  
Chromosome 1 ◽  
Coding Sequences ◽  
Resistance Phenotype ◽  
Resistance Reaction

Two resistance phenotypes to P. brassicae have been found in A. thaliana. A first resistance phenotype has been detected to the isolate 'e<sub>2</sub>' and is polygenically inherited. The second resistance to isolate 'e<sub>3</sub>' is caused by the dominant resistance gene RPB1. By crossing no influence could be shown for salicylic acid, jasmonic acid and ethylene in the latter resistance reaction. The RPB1 locus was narrowed down to 71 kb on chromosome 1, where three pseudogenes and 13 coding sequences are located. Six of them showed cosegregation with RPB1. None of these sequences have similarities to identified resistance genes or other known genes. Ten coding sequences were expressed, but CDS9 was exclusively expressed in the resistant ecotype Tsu-0.

scholarly journals Genome-wide transcriptomic analysis reveals correlation between higher WRKY61 expression and reduced symptom severity in Turnip crinkle virus infected Arabidopsis thaliana

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Ruimin Gao ◽  
Peng Liu ◽  
Yuhan Yong ◽  
Sek-Man Wong
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcriptome Analysis ◽  
Plant Immunity ◽  
Plant Defence ◽  
Enrichment Analysis ◽  
Global Gene Expression ◽  
Chemical Stimulus ◽  
Turnip Crinkle Virus ◽  
Rna Seq ◽  
Genome Wide

Abstract Turnip crinkle virus (TCV) is a carmovirus that infects many Arabidopsis ecotypes. Most studies mainly focused on discovery of resistance genes against TCV infection and there is no Next Generation Sequencing based comparative genome wide transcriptome analysis reported. In this study, RNA-seq based transcriptome analysis revealed that 238 (155 up-regulated and 83 down-regulated) significant differentially expressed genes with at least 15-fold change were determined. Fifteen genes (including upregulated, unchanged and downregulated) were selected for RNA-seq data validation using quantitative real-time PCR, which showed consistencies between these two sets of data. GO enrichment analysis showed that numerous terms such as stress, immunity, defence and chemical stimulus were affected in TCV-infected plants. One putative plant defence related gene named WRKY61 was selected for further investigation. It showed that WRKY61 overexpression plants displayed reduced symptoms and less virus accumulation, as compared to wild type (WT) and WRKY61 deficient lines, suggesting that higher WRKY61 expression level reduced TCV viral accumulation. In conclusion, our transcriptome analysis showed that global gene expression was detected in TCV-infected Arabidopsis thaliana. WRKY61 gene was shown to be negatively correlated with TCV infection and viral symptoms, which may be connected to plant immunity pathways.

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