scholarly journals The impact of the absence of aliphatic glucosinolates on water transport under salt stress in Arabidopsis thaliana

2015 ◽  
Vol 6 ◽  
Author(s):  
Mcarmen Martínez-Ballesta ◽  
Diego A. Moreno-Fernández ◽  
Diego Castejón ◽  
Cristina Ochando ◽  
Piero A. Morandini ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Water Transport ◽  
Aliphatic Glucosinolates ◽  
The Impact

scholarly journals Influence of Salt Stress on the flg22 Induced ROS Production in Arabidopsis Thaliana Leaves

2019 ◽  
Author(s):  
Amrahov Nurlan Rashid ◽  
Martin Janda ◽  
Mammadov Ziaddin Mahmud ◽  
Olga Valentová ◽  
Lenka Burketová ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Leaf Tissue ◽  
Tissue Response ◽  
Combined Effect ◽  
Stress Factors ◽  
Ros Production ◽  
High Concentration ◽  
Natural Habitats ◽  
The Impact

AbstractIn their natural habitats, plants have to cope with multiple stress factors triggering respective response pathways, leading to mutual interference. Our work aimed to study the effect of salt stress in combination with immune response triggered by microbe-associated molecular pattern (MAMP) in Arabidopsis thaliana Col-0 plants. We measured ROS production after treatment with flg22 and the influence of concomitant salt stress (NaCl and Na2CO3).The maximum combined effect of NaCl solution and flg22 on ROS production was achieved at 6 mM salt, which was almost 2 times higher than the single effect of MAMP. A similar maximum combined effect with Na2CO3 was observed at 10 mM concentration. High concentration of NaCl and Na2CO3 was accompanied with declining of ROS production, which was completely inhibited at 150 mM of NaCl and at 50 mM of Na2CO3.The immediate and long term (24 h) effect of NaCl on leaf tissue of Arabidopsis thaliana showed that the impact of salt stress on flg22induced ROS production probably did not affect the genetic aspects of the tissue response, but was associated with ionic and osmotic stresses. Experiments with mannitol, KCl and CaCl2 allowed to conclude that the observed effect was due to the ionic stress of the salt rather than the osmotic one.

scholarly journals Oxidative and Salt Stresses Alter the 26S Proteasome Holoenzyme and Associated Protein Profiles in Arabidopsis Thaliana

2021 ◽  
Author(s):  
Diana Bonea ◽  
Jenan Noureddine ◽  
Sonia Gazzarrini ◽  
Rongmin Zhao
Keyword(s):  
Oxidative Stress ◽  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Seed Coat ◽  
Abiotic Stresses ◽  
Stress Sensitivity ◽  
26S Proteasome ◽  
Free Form ◽  
The Impact

Abstract Background: The 26S proteasome, canonically composed of multi-subunit 19S regulatory (RP) and 20S core (CP) particles, is crucial for cellular proteostasis. Proteasomes may be re-modeled, activated, or re-localized and this regulation is critical for plants in response to environmental stresses. The proteasome holoenzyme assembly and dissociation are therefore highly dynamic in vivo. However, the stoichiometric changes of the plant proteasomes and how proteasome associated chaperones vary under common abiotic stresses have not been systematically studied.Results: Here, we studied the impact of abiotic stresses on proteasome structure, activity, and interacting partners in Arabidopsis thaliana. We analyzed available RNA expression data and observed that expressions of proteasome coding genes varied substantially under stresses; however, the protein levels of a few key subunits did not change significantly within 24 hours. Instead, a switch in the predominant proteasome complex, from 26S to 20S, occurs under oxidative or salt stress. Oxidative stress also reduced the cellular ATP content and the association of HSP70-family proteins to the 20S proteasome, but enhanced the activity of cellular free form CP. Salt stress, on the other hand, did not affect cellular ATP level, but caused subtle changes in proteasome subunit composition and impacted bindings of assembly chaperones. Analyses of an array of T-DNA insertional mutant lines highlighted important roles for several putative assembly chaperones in seedling establishment and stress sensitivity. We also observed that knockout of PBAC1, one of the a-ring assembly chaperones, resulted in hypersensitivity and tearing of the seed coat during sterilization. Conclusions: Our study revealed an evolutionarily conserved mechanism of proteasome regulation during oxidative stress, involving dynamic regulation of the holoenzyme formation and associated regulatory proteins, and we also identified a novel role of the PBAC1 proteasome assembly chaperone in seed coat development.

scholarly journals Oxidative and salt stresses alter the 26S proteasome holoenzyme and associated protein profiles in Arabidopsis thaliana

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Diana Bonea ◽  
Jenan Noureddine ◽  
Sonia Gazzarrini ◽  
Rongmin Zhao
Keyword(s):  
Oxidative Stress ◽  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Seed Coat ◽  
Abiotic Stresses ◽  
Stress Sensitivity ◽  
26S Proteasome ◽  
Free Form ◽  
The Impact

Abstract Background The 26S proteasome, canonically composed of multi-subunit 19S regulatory (RP) and 20S core (CP) particles, is crucial for cellular proteostasis. Proteasomes are re-modeled, activated, or re-localized and this regulation is critical for plants in response to environmental stresses. The proteasome holoenzyme assembly and dissociation are therefore highly dynamic in vivo. However, the stoichiometric changes of the plant proteasomes and how proteasome associated chaperones vary under common abiotic stresses have not been systematically studied. Results Here, we studied the impact of abiotic stresses on proteasome structure, activity, and interacting partners in Arabidopsis thaliana. We analyzed available RNA expression data and observed that expressions of proteasome coding genes varied substantially under stresses; however, the protein levels of a few key subunits did not change significantly within 24 h. Instead, a switch in the predominant proteasome complex, from 26S to 20S, occurs under oxidative or salt stress. Oxidative stress also reduced the cellular ATP content and the association of HSP70-family proteins to the 20S proteasome, but enhanced the activity of cellular free form CP. Salt stress, on the other hand, did not affect cellular ATP level, but caused subtle changes in proteasome subunit composition and impacted bindings of assembly chaperones. Analyses of an array of T-DNA insertional mutant lines highlighted important roles for several putative assembly chaperones in seedling establishment and stress sensitivity. We also observed that knockout of PBAC1, one of the α-ring assembly chaperones, resulted in reduced germination and tearing of the seed coat following sterilization. Conclusions Our study revealed an evolutionarily conserved mechanism of proteasome regulation during oxidative stress, involving dynamic regulation of the holoenzyme formation and associated regulatory proteins, and we also identified a novel role of the PBAC1 proteasome assembly chaperone in seed coat development.

scholarly journals Analysis of Arabidopsis thaliana HKT1 and Eutrema salsugineum/botschantzevii HKT1;2 Promoters in Response to Salt Stress in Athkt1:1 Mutant

2019 ◽  
Vol 61 (6) ◽  
pp. 442-450 ◽  
Author(s):  
Ismat Nawaz ◽  
Mazhar Iqbal ◽  
Henk W. J. Hakvoort ◽  
Albertus H. de Boer ◽  
Henk Schat
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Eutrema Salsugineum

ALKBH10B , an m 6 A mRNA demethylase, plays a role in salt stress and ABA responses in Arabidopsis thaliana

2021 ◽  
Author(s):  
Yasira Shoaib ◽  
Jianzhong Hu ◽  
Stefano Manduzio ◽  
Hunseung Kang
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress

scholarly journals Physiological Responses of Salinized Fenugreek (Trigonellafoenum-graecum L.) Plants to Foliar Application of Salicylic Acid

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 657
Author(s):  
Reda E. Abdelhameed ◽  
Arafat Abdel Hamed Abdel Latef ◽  
Rania S. Shehata
Keyword(s):  
Salicylic Acid ◽  
Salt Stress ◽  
Foliar Application ◽  
Shikimic Acid ◽  
Plant Tolerance ◽  
Physiological Mechanisms ◽  
Content Index ◽  
Malondialdehyde Content ◽  
Total Free Amino Acids ◽  
The Impact

Considering the detrimental effects of salt stress on the physiological mechanisms of plants in terms of growth, development and productivity, intensive efforts are underway to improve plant tolerance to salinity. Hence, an experiment was conducted to assess the impact of the foliar application of salicylic acid (SA; 0.5 mM) on the physiological traits of fenugreek (Trigonellafoenum-graecum L.) plants grown under three salt concentrations (0, 75, and 150 mM NaCl). An increase in salt concentration generated a decrease in the chlorophyll content index (CCI); however, the foliar application of SA boosted the CCI. The malondialdehyde content increased in salt-stressed fenugreek plants, while a reduction in content was observed with SA. Likewise, SA application induced an accumulation of proline, total phenolics, and flavonoids. Moreover, further increases in total free amino acids and shikimic acid were observed with the foliar application of SA, in either control or salt-treated plants. Similar results were obtained for ascorbate peroxidase, peroxidase, polyphenol oxidase, and catalase with SA application. Hence, we concluded that the foliar application of SA ameliorates salinity, and it is a growth regulator that improves the tolerance of fenugreek plants under salt stress.

MYB3R-mediated active repression of cell cycle and growth under salt stress in Arabidopsis thaliana

2021 ◽  
Author(s):  
Toru Okumura ◽  
Yuji Nomoto ◽  
Kosuke Kobayashi ◽  
Takamasa Suzuki ◽  
Hirotomo Takatsuka ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Arabidopsis Thaliana ◽  
Salt Stress

Comparative Quantitative Trait Loci Mapping of Aliphatic, Indolic and Benzylic Glucosinolate Production in Arabidopsis thaliana Leaves and Seeds

Genetics ◽  
2001 ◽  
Vol 159 (1) ◽  
pp. 359-370 ◽  
Author(s):  
Daniel J Kliebenstein ◽  
Jonathan Gershenzon ◽  
Thomas Mitchell-Olds
Keyword(s):  
Arabidopsis Thaliana ◽  
Secondary Metabolites ◽  
Plant Environment ◽  
Aliphatic Glucosinolates ◽  
Glucosinolate Concentration ◽  
Indolic Glucosinolates ◽  
The Individual ◽  
Concentration Mapping ◽  
Indolic Glucosinolate ◽  
Aliphatic Glucosinolate

Abstract Secondary metabolites are a diverse set of plant compounds believed to have numerous functions in plant-environment interactions. Despite this importance, little is known about the regulation of secondary metabolite accumulation. We are studying the regulation of glucosinolates, a large group of secondary metabolites, in Arabidopsis to investigate how secondary metabolism is controlled. We utilized Ler and Cvi, two ecotypes of Arabidopsis that have striking differences in both the types and amounts of glucosinolates that accumulate in the seeds and leaves. QTL analysis identified six loci determining total aliphatic glucosinolate accumulation, six loci controlling total indolic glucosinolate concentration, and three loci regulating benzylic glucosinolate levels. Our results show that two of the loci controlling total aliphatic glucosinolates map to biosynthetic loci that interact epistatically to regulate aliphatic glucosinolate accumulation. In addition to the six loci regulating total indolic glucosinolate concentration, mapping of QTL for the individual indolic glucosinolates identified five additional loci that were specific to subsets of the indolic glucosinolates. These data show that there are a large number of variable loci controlling glucosinolate accumulation in Arabidopsis thaliana.

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