scholarly journals Influence of Salt Stress on the flg22 Induced ROS Production in Arabidopsis Thaliana Leaves

2019 ◽  
Author(s):  
Amrahov Nurlan Rashid ◽  
Martin Janda ◽  
Mammadov Ziaddin Mahmud ◽  
Olga Valentová ◽  
Lenka Burketová ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Leaf Tissue ◽  
Tissue Response ◽  
Combined Effect ◽  
Stress Factors ◽  
Ros Production ◽  
High Concentration ◽  
Natural Habitats ◽  
The Impact

AbstractIn their natural habitats, plants have to cope with multiple stress factors triggering respective response pathways, leading to mutual interference. Our work aimed to study the effect of salt stress in combination with immune response triggered by microbe-associated molecular pattern (MAMP) in Arabidopsis thaliana Col-0 plants. We measured ROS production after treatment with flg22 and the influence of concomitant salt stress (NaCl and Na2CO3).The maximum combined effect of NaCl solution and flg22 on ROS production was achieved at 6 mM salt, which was almost 2 times higher than the single effect of MAMP. A similar maximum combined effect with Na2CO3 was observed at 10 mM concentration. High concentration of NaCl and Na2CO3 was accompanied with declining of ROS production, which was completely inhibited at 150 mM of NaCl and at 50 mM of Na2CO3.The immediate and long term (24 h) effect of NaCl on leaf tissue of Arabidopsis thaliana showed that the impact of salt stress on flg22induced ROS production probably did not affect the genetic aspects of the tissue response, but was associated with ionic and osmotic stresses. Experiments with mannitol, KCl and CaCl2 allowed to conclude that the observed effect was due to the ionic stress of the salt rather than the osmotic one.

Nitric Oxide Enhances Salt Tolerance through Regulating Antioxidant Enzyme Activity and Nutrient Uptake in Pea

2020 ◽  
Author(s):  
Esin Dadasoglu ◽  
Melek Ekinci ◽  
Raziye Kul ◽  
Mostafakamal Shams ◽  
Metin Turan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Enzyme Activity ◽  
Salt Stress ◽  
Nutrient Uptake ◽  
Antioxidant Enzyme ◽  
Crop Production ◽  
Electrical Conductance ◽  
Antioxidant Enzyme Activity ◽  
Stress Factors ◽  
The Impact

Background: Salinity is one of the environmental stress factors that restrict the crop production by endangering agricultural areas. Nitric oxide (NO) protects plants from damage caused by oxidative stress conditions in various biological ways. Methods: In this greenhouse investigation during 2018, pea plants were irrigated with three levels of NaCl (0, 50 and 100 mM) solutions. NO solutions were prepared with three different doses (0, 75 and 100 µM SNP). These solutions were applied to the seeds before sowing and then to the leaves of the pea cultivars. The study was conducted to analyze the impact of NO on growth, malondialdehyde (MDA), hydrogen peroxide (H2O2), antioxidant enzyme activity and nutrient uptake in two pea cultivars under salinity conditions. Result: Salinity reduced fresh-dry weight, relative water content (RWC), and chlorophyll a and b content of pea. However, NO enhanced these parameters under salt stress. Salinity increased tissue electrical conductance (TEC), H2O2 and MDA content, which were decreased by combined application of NaCl and NO. Salinity caused an increase in antioxidant enzyme activity in pea and NO made a significant improvement in their activities under salinity conditions. Salinity treatments decreased the ratio of K+/Na+ and Ca2+/Na+ in both cultivars, and application of NO elevated them as compared to the control under salt stress. In conclude, exogenous NO treatment could help pea to tolerate salinity stress by increasing the chlorophyll content and regulating antioxidant enzyme activity and nutrient uptake.

scholarly journals Influence of salt stress on the flg22 induced ROS production in Arabidopsis thaliana leaves

2021 ◽  
Vol 53 (5) ◽  
Author(s):  
Amrahov Nurlan Rashid ◽  
Martin Janda ◽  
Mammadov Ziaddin Mahmud ◽  
Olga Valentová ◽  
Burketová Lenka ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Ros Production

scholarly journals Altered Root Growth, Auxin Metabolism and Distribution in Arabidopsis thaliana Exposed to Salt and Osmotic Stress

2021 ◽  
Vol 22 (15) ◽  
pp. 7993
Author(s):  
Ana Smolko ◽  
Nataša Bauer ◽  
Iva Pavlović ◽  
Aleš Pěnčík ◽  
Ondřej Novák ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Osmotic Stress ◽  
Root Growth ◽  
Plant Root ◽  
Stress Factors ◽  
Quiescent Center ◽  
Gene Expressions ◽  
Auxin Distribution ◽  
Salt And Osmotic Stress

Salt and osmotic stress are the main abiotic stress factors affecting plant root growth and architecture. We investigated the effect of salt (100 mM NaCl) and osmotic (200 mM mannitol) stress on the auxin metabolome by UHPLC-MS/MS, auxin distribution by confocal microscopy, and transcript levels of selected genes by qRT-PCR in Arabidopsis thaliana ecotype Columbia-0 (Col-0) and DR5rev::GFP (DR5) line. During long-term stress (13 days), a stability of the auxin metabolome and a tendency to increase indole-3-acetic acid (IAA) were observed, especially during salt stress. Short-term stress (3 h) caused significant changes in the auxin metabolome, especially NaCl treatment resulted in a significant reduction of IAA. The data derived from auxin profiling were consistent with gene expressions showing the most striking changes in the transcripts of YUC, GH3, and UGT transcripts, suggesting disruption of auxin biosynthesis, but especially in the processes of amide and ester conjugation. These data were consistent with the auxin distribution observed in the DR5 line. Moreover, NaCl treatment caused a redistribution of auxin signals from the quiescent center and the inner layers of the root cap to the epidermal and cortical cells of the root elongation zone. The distribution of PIN proteins was also disrupted by salt stress; in particular, PIN2 was suppressed, even after 5 min of treatment. Based on our results, the DR5 line was more sensitive to the applied stresses than Col-0, although both lines showed similar trends in root morphology, as well as transcriptome and metabolome parameters under stress conditions.

scholarly journals The impact of the absence of aliphatic glucosinolates on water transport under salt stress in Arabidopsis thaliana

2015 ◽  
Vol 6 ◽  
Author(s):  
Mcarmen Martínez-Ballesta ◽  
Diego A. Moreno-Fernández ◽  
Diego Castejón ◽  
Cristina Ochando ◽  
Piero A. Morandini ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Water Transport ◽  
Aliphatic Glucosinolates ◽  
The Impact

scholarly journals Oxidative and Salt Stresses Alter the 26S Proteasome Holoenzyme and Associated Protein Profiles in Arabidopsis Thaliana

2021 ◽  
Author(s):  
Diana Bonea ◽  
Jenan Noureddine ◽  
Sonia Gazzarrini ◽  
Rongmin Zhao
Keyword(s):  
Oxidative Stress ◽  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Seed Coat ◽  
Abiotic Stresses ◽  
Stress Sensitivity ◽  
26S Proteasome ◽  
Free Form ◽  
The Impact

Abstract Background: The 26S proteasome, canonically composed of multi-subunit 19S regulatory (RP) and 20S core (CP) particles, is crucial for cellular proteostasis. Proteasomes may be re-modeled, activated, or re-localized and this regulation is critical for plants in response to environmental stresses. The proteasome holoenzyme assembly and dissociation are therefore highly dynamic in vivo. However, the stoichiometric changes of the plant proteasomes and how proteasome associated chaperones vary under common abiotic stresses have not been systematically studied.Results: Here, we studied the impact of abiotic stresses on proteasome structure, activity, and interacting partners in Arabidopsis thaliana. We analyzed available RNA expression data and observed that expressions of proteasome coding genes varied substantially under stresses; however, the protein levels of a few key subunits did not change significantly within 24 hours. Instead, a switch in the predominant proteasome complex, from 26S to 20S, occurs under oxidative or salt stress. Oxidative stress also reduced the cellular ATP content and the association of HSP70-family proteins to the 20S proteasome, but enhanced the activity of cellular free form CP. Salt stress, on the other hand, did not affect cellular ATP level, but caused subtle changes in proteasome subunit composition and impacted bindings of assembly chaperones. Analyses of an array of T-DNA insertional mutant lines highlighted important roles for several putative assembly chaperones in seedling establishment and stress sensitivity. We also observed that knockout of PBAC1, one of the a-ring assembly chaperones, resulted in hypersensitivity and tearing of the seed coat during sterilization. Conclusions: Our study revealed an evolutionarily conserved mechanism of proteasome regulation during oxidative stress, involving dynamic regulation of the holoenzyme formation and associated regulatory proteins, and we also identified a novel role of the PBAC1 proteasome assembly chaperone in seed coat development.

scholarly journals Oxidative and salt stresses alter the 26S proteasome holoenzyme and associated protein profiles in Arabidopsis thaliana

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Diana Bonea ◽  
Jenan Noureddine ◽  
Sonia Gazzarrini ◽  
Rongmin Zhao
Keyword(s):  
Oxidative Stress ◽  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Seed Coat ◽  
Abiotic Stresses ◽  
Stress Sensitivity ◽  
26S Proteasome ◽  
Free Form ◽  
The Impact

Abstract Background The 26S proteasome, canonically composed of multi-subunit 19S regulatory (RP) and 20S core (CP) particles, is crucial for cellular proteostasis. Proteasomes are re-modeled, activated, or re-localized and this regulation is critical for plants in response to environmental stresses. The proteasome holoenzyme assembly and dissociation are therefore highly dynamic in vivo. However, the stoichiometric changes of the plant proteasomes and how proteasome associated chaperones vary under common abiotic stresses have not been systematically studied. Results Here, we studied the impact of abiotic stresses on proteasome structure, activity, and interacting partners in Arabidopsis thaliana. We analyzed available RNA expression data and observed that expressions of proteasome coding genes varied substantially under stresses; however, the protein levels of a few key subunits did not change significantly within 24 h. Instead, a switch in the predominant proteasome complex, from 26S to 20S, occurs under oxidative or salt stress. Oxidative stress also reduced the cellular ATP content and the association of HSP70-family proteins to the 20S proteasome, but enhanced the activity of cellular free form CP. Salt stress, on the other hand, did not affect cellular ATP level, but caused subtle changes in proteasome subunit composition and impacted bindings of assembly chaperones. Analyses of an array of T-DNA insertional mutant lines highlighted important roles for several putative assembly chaperones in seedling establishment and stress sensitivity. We also observed that knockout of PBAC1, one of the α-ring assembly chaperones, resulted in reduced germination and tearing of the seed coat following sterilization. Conclusions Our study revealed an evolutionarily conserved mechanism of proteasome regulation during oxidative stress, involving dynamic regulation of the holoenzyme formation and associated regulatory proteins, and we also identified a novel role of the PBAC1 proteasome assembly chaperone in seed coat development.

Factors Influencing Academic Stress on Students of University of Economics and Business - Vietnam National University

2020 ◽  
Vol 36 (3) ◽  
Author(s):  
Nham Phong Tuan ◽  
Nguyen Ngoc Quy ◽  
Nguyen Thi Thanh Huyen ◽  
Hong Tra My ◽  
Tran Nhu Phu
Keyword(s):  
Academic Stress ◽  
Stress Factors ◽  
Negative Effects ◽  
Cross Sectional ◽  
Stress Scale ◽  
Perceptions And Attitudes ◽  
National University ◽  
Questionnaire Surveys ◽  
The University ◽  
The Impact

The objective of this study is to investigate the impact of seven factors causing academic stress on students of University of Economics and Business - Vietnam National University: Lack of leisure time, Academic performance, Fear of failure, Academic overload, Finances, Competition between students, Relationships with university faculty. Based on the results of a practical survey of 185 students who are attending any courses at the University of Economics and Business - Vietnam National University, the study assesses the impact of stress factors on students. The thesis focuses on clarifying the concept of "stress" and the stress level of students, while pointing out its negative effects on students. This study includes two cross-sectional questionnaire surveys. The first survey uses a set of 16 questions to assess students’ perceptions and attitudes based on an instrument to measure academic stress - Educational Stress Scale for Adolescents (ESSA). The second survey aims to test internal consistency, the robustness of the previously established 7-factor structure. Henceforth, the model was brought back and used qualitatively, combined with Cronbach’s Alpha measurement test and EFA discovery factor analysis. This study was conducted from October 2019 to December 2019. From these practical analyzes, several proposals were made for the society, the school and the students themselves.

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