scholarly journals Transcriptional responses of Arabidopsis thaliana to chewing and sucking insect herbivores

2014 ◽  
Vol 5 ◽  
Author(s):  
Heidi M. Appel ◽  
Howard Fescemyer ◽  
Juergen Ehlting ◽  
David Weston ◽  
Erin Rehrig ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Insect Herbivores ◽  
Transcriptional Responses

scholarly journals AtRsgA from Arabidopsis thaliana controls maturation of the small subunit of the chloroplast ribosome

2017 ◽  
Author(s):  
Marcin Janowski ◽  
Reimo Zoschke ◽  
Lars Scharff ◽  
Silvia Martinez Jaime ◽  
Camilla Ferrari ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Proteomic Analysis ◽  
Ribosome Biogenesis ◽  
Functional Characterization ◽  
Small Subunit ◽  
Signalling Pathways ◽  
Compensatory Mechanism ◽  
Transcriptional Responses ◽  
Retrograde Signalling ◽  
Assembly Factor

SummaryPlastid ribosomes are very similar in structure and function to ribosomes of their bacterial ancestors. Since ribosome biogenesis is not thermodynamically favourable at biological conditions, it requires activity of many assembly factors. Here, we have characterized a homolog of bacterial rsgA in Arabidopsis thaliana and show that it can complement the bacterial homolog. Functional characterization of a strong mutant in Arabidopsis revealed that the protein is essential for plant viability, while a weak mutant produced dwarf, chlorotic plants that incorporated immature pre-16S ribosomal RNA into translating ribosomes. Physiological analysis of the mutant plants revealed smaller, but more numerous chloroplasts in the mesophyll cells, reduction of chlorophyll a and b, depletion of proplastids from the rib meristem and decreased photosynthetic electron transport rate and efficiency. Comparative RNA-sequencing and proteomic analysis of the weak mutant and wild-type plants revealed that various biotic stress-related, transcriptional regulation and post-transcriptional modification pathways were repressed in the mutant. Intriguingly, while nuclear- and chloroplast-encoded photosynthesis-related proteins were less abundant in the mutant, the corresponding transcripts were upregulated, suggesting an elaborate compensatory mechanism, potentially via differentially active retrograde signalling pathways. To conclude, this study reveals a new chloroplast ribosome assembly factor and outlines the transcriptomic and proteomic responses of the compensatory mechanism activated during decreased chloroplast function.Significance statementAtRsgA is an assembly factor necessary for maturation of the small subunit of the chloroplast ribosome. Depletion of AtRsgA leads to dwarfed, chlorotic plants and smaller, but more numerous chloroplasts. Large-scale transcriptomic and proteomic analysis revealed that chloroplast-encoded and - targeted proteins were less abundant, while the corresponding transcripts were upregulated in the mutant. We analyse the transcriptional responses of several retrograde signalling pathways to suggest a mechanism underlying this compensatory response.

scholarly journals Transcriptional responses of Arabidopsis thaliana to oil contamination

2016 ◽  
Vol 127 ◽  
pp. 63-72 ◽  
Author(s):  
Sarah Muniz Nardeli ◽  
Carolina Farias Saad ◽  
Priscilla de Barros Rossetto ◽  
Vanessa Santana Caetano ◽  
Marcelo Ribeiro-Alves ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Oil Contamination ◽  
Transcriptional Responses

Fluorescence Reports Intact Quantum Dot Uptake into Roots and Translocation to Leaves of Arabidopsis thaliana and Subsequent Ingestion by Insect Herbivores

2014 ◽  
Vol 49 (1) ◽  
pp. 626-632 ◽  
Author(s):  
Yeonjong Koo ◽  
Jing Wang ◽  
Qingbo Zhang ◽  
Huiguang Zhu ◽  
E. Wassim Chehab ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Quantum Dot ◽  
Insect Herbivores

scholarly journals Transcriptional responses of Arabidopsis thaliana ecotypes with different glucosinolate profiles after attack by polyphagous Myzus persicae and oligophagous Brevicoryne brassicae

2007 ◽  
Vol 58 (10) ◽  
pp. 2537-2552 ◽  
Author(s):  
Anna Kuśnierczyk ◽  
Per Winge ◽  
Herman Midelfart ◽  
W. Scott Armbruster ◽  
John T. Rossiter ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Myzus Persicae ◽  
Brevicoryne Brassicae ◽  
Transcriptional Responses

scholarly journals Transcriptional Responses of Arabidopsis thaliana during Wilt Disease Caused by the Soil-Borne Phytopathogenic Bacterium, Ralstonia solanacearum

PLoS ONE ◽  
2008 ◽  
Vol 3 (7) ◽  
pp. e2589 ◽  
Author(s):  
Jian Hu ◽  
Xavier Barlet ◽  
Laurent Deslandes ◽  
Judith Hirsch ◽  
Dong Xin Feng ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Ralstonia Solanacearum ◽  
Wilt Disease ◽  
Transcriptional Responses ◽  
Phytopathogenic Bacterium

scholarly journals Comparative Analysis of Quantitative Trait Loci Controlling Glucosinolates, Myrosinase and Insect Resistance in Arabidopsis thaliana

Genetics ◽  
2002 ◽  
Vol 161 (1) ◽  
pp. 325-332 ◽  
Author(s):  
Daniel Kliebenstein ◽  
Deana Pedersen ◽  
Bridget Barker ◽  
Thomas Mitchell-Olds
Keyword(s):  
Arabidopsis Thaliana ◽  
Trichoplusia Ni ◽  
Insect Herbivory ◽  
Chemical Defenses ◽  
Insect Herbivores ◽  
Specific Chemical ◽  
Chemical Structures ◽  
Plant Chemical ◽  
Breakdown Rates ◽  
Plant Chemical Defenses

Abstract Evolutionary interactions among insect herbivores and plant chemical defenses have generated systems where plant compounds have opposing fitness consequences for host plants, depending on attack by various insect herbivores. This interplay complicates understanding of fitness costs and benefits of plant chemical defenses. We are studying the role of the glucosinolate-myrosinase chemical defense system in protecting Arabidopsis thaliana from specialist and generalist insect herbivory. We used two Arabidopsis recombinant inbred populations in which we had previously mapped QTL controlling variation in the glucosinolate-myrosinase system. In this study we mapped QTL controlling resistance to specialist (Plutella xylostella) and generalist (Trichoplusia ni) herbivores. We identified a number of QTL that are specific to one herbivore or the other, as well as a single QTL that controls resistance to both insects. Comparison of QTL for herbivory, glucosinolates, and myrosinase showed that T. ni herbivory is strongly deterred by higher glucosinolate levels, faster breakdown rates, and specific chemical structures. In contrast, P. xylostella herbivory is uncorrelated with variation in the glucosinolate-myrosinase system. This agrees with evolutionary theory stating that specialist insects may overcome host plant chemical defenses, whereas generalists will be sensitive to these same defenses.

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