scholarly journals RNA-Seq Comparison of Larval and Adult Malpighian Tubules of the Yellow Fever Mosquito Aedes aegypti Reveals Life Stage-Specific Changes in Renal Function

2017 ◽  
Vol 8 ◽  
Author(s):  
Yiyi Li ◽  
Peter M. Piermarini ◽  
Carlos J. Esquivel ◽  
Hannah E. Drumm ◽  
Faye D. Schilkey ◽  
...  
Keyword(s):  
Renal Function ◽  
Aedes Aegypti ◽  
Yellow Fever ◽  
Life Stage ◽  
Malpighian Tubules ◽  
Rna Seq ◽  
Yellow Fever Mosquito

scholarly journals Localization and role of inward rectifier K+ channels in Malpighian tubules of the yellow fever mosquito Aedes aegypti

2015 ◽  
Vol 67 ◽  
pp. 59-73 ◽  
Author(s):  
Peter M. Piermarini ◽  
Sonja M. Dunemann ◽  
Matthew F. Rouhier ◽  
Travis L. Calkins ◽  
Rene Raphemot ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Yellow Fever ◽  
Inward Rectifier ◽  
Malpighian Tubules ◽  
Yellow Fever Mosquito ◽  
K Channels

scholarly journals NHE8 is an intracellular cation/H+ exchanger in renal tubules of the yellow fever mosquito Aedes aegypti

2009 ◽  
Vol 296 (4) ◽  
pp. F730-F750 ◽  
Author(s):  
Peter M. Piermarini ◽  
Dirk Weihrauch ◽  
Heiko Meyer ◽  
Markus Huss ◽  
Klaus W. Beyenbach
Keyword(s):  
Aedes Aegypti ◽  
Yellow Fever ◽  
Xenopus Oocytes ◽  
Intracellular Localization ◽  
Malpighian Tubules ◽  
Xenopus Laevis Oocytes ◽  
Renal Tubules ◽  
Yellow Fever Mosquito ◽  
Western Blots ◽  
Principal Cells

The goal of this study was to identify and characterize the hypothesized apical cation/H+ exchanger responsible for K+ and/or Na+ secretion in the renal (Malpighian) tubules of the yellow fever mosquito Aedes aegypti. From Aedes Malpighian tubules, we cloned “ AeNHE8,” a full-length cDNA encoding an ortholog of mammalian Na+/H+ exchanger 8 (NHE8). The expression of AeNHE8 transcripts is ubiquitous among mosquito tissues and is not enriched in Malpighian tubules. Western blots of Malpighian tubules suggest that AeNHE8 is expressed primarily as an intracellular protein, which was confirmed by immunohistochemical localizations in Malpighian tubules. AeNHE8 immunoreactivity is expressed in principal cells of the secretory, distal segments, where it localizes to a subapical compartment (e.g., vesicles or endosomes), but not in the apical brush border. Furthermore, feeding mosquitoes a blood meal or treating isolated tubules with dibutyryl-cAMP, both of which stimulate a natriuresis by Malpighian tubules, do not influence the intracellular localization of AeNHE8 in principal cells. When expressed heterologously in Xenopus laevis oocytes, AeNHE8 mediates EIPA-sensitive Na/H exchange, in which Li+ partially and K+ poorly replace Na+. The expression of AeNHE8 in Xenopus oocytes is associated with the development of a conductive pathway that closely resembles the known endogenous nonselective cation conductances of Xenopus oocytes. In conclusion, AeNHE8 does not mediate cation/H+ exchange in the apical membrane of Aedes Malpighian tubules; it is more likely involved with an intracellular function.

Effects of amiloride on isolated Malpighian tubules of the yellow fever mosquito (Aedes aegypti)

1992 ◽  
Vol 38 (5) ◽  
pp. 329-337 ◽  
Author(s):  
J.L. Hegarty ◽  
B. Zhang ◽  
M.C. Carroll ◽  
E.J. Cragoe ◽  
K.W. Beyenbach
Keyword(s):  
Aedes Aegypti ◽  
Yellow Fever ◽  
Malpighian Tubules ◽  
Yellow Fever Mosquito
Sign in / Sign up

Export Citation Format

Share Document