scholarly journals A Blood Meal Enhances Innexin mRNA Expression in the Midgut, Malpighian Tubules, and Ovaries of the Yellow Fever Mosquito Aedes aegypti

Insects ◽  
2017 ◽  
Vol 8 (4) ◽  
pp. 122 ◽  
Author(s):  
◽  
Keyword(s):  
Aedes Aegypti ◽  
Mrna Expression ◽  
Yellow Fever ◽  
Blood Meal ◽  
Malpighian Tubules ◽  
Yellow Fever Mosquito

scholarly journals The Fat Body Transcriptomes of the Yellow Fever Mosquito Aedes aegypti, Pre- and Post- Blood Meal

PLoS ONE ◽  
2011 ◽  
Vol 6 (7) ◽  
pp. e22573 ◽  
Author(s):  
David P. Price ◽  
Vijayaraj Nagarajan ◽  
Alexander Churbanov ◽  
Peter Houde ◽  
Brook Milligan ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Yellow Fever ◽  
Blood Meal ◽  
Fat Body ◽  
Yellow Fever Mosquito

scholarly journals Knockout of juvenile hormone receptor, Methoprene-tolerant, induces black larval phenotype in the yellow fever mosquito, Aedes aegypti

2019 ◽  
Vol 116 (43) ◽  
pp. 21501-21507 ◽  
Author(s):  
Guan-Heng Zhu ◽  
Yaoyu Jiao ◽  
Shankar C. R. R. Chereddy ◽  
Mi Young Noh ◽  
Subba Reddy Palli
Keyword(s):  
Aedes Aegypti ◽  
Larval Development ◽  
Yellow Fever ◽  
Blood Meal ◽  
Mode Of Action ◽  
Human Pathogens ◽  
Yellow Fever Mosquito ◽  
Guide Rna ◽  
Larval Stages ◽  
Blood Meal Digestion

The yellow fever mosquito, Aedes aegypti, vectors human pathogens. Juvenile hormones (JH) control almost every aspect of an insect’s life, and JH analogs are currently used to control mosquito larvae. Since RNA interference does not work efficiently during the larval stages of this insect, JH regulation of larval development and mode of action of JH analogs are not well studied. To overcome this limitation, we used a multiple single guide RNA-based CRISPR/Cas9 genome-editing method to knockout the methoprene-tolerant (Met) gene coding for a JH receptor. The Met knockout larvae exhibited a black larval phenotype during the L3 (third instar larvae) and L4 (fourth instar larvae) stages and died before pupation. However, Met knockout did not affect embryonic development or the L1 and L2 stages. Microscopy studies revealed the precocious synthesis of a dark pupal cuticle during the L3 and L4 stages. Gene expression analysis showed that Krüppel homolog 1, a key transcription factor in JH action, was down-regulated, but genes coding for proteins involved in melanization, pupal and adult cuticle synthesis, and blood meal digestion in adults were up-regulated in L4 Met mutants. These data suggest that, during the L3 and L4 stages, Met mediates JH suppression of pupal/adult genes involved in the synthesis and melanization of the cuticle and blood meal digestion. These results help to advance our knowledge of JH regulation of larval development and the mode of action of JH analogs in Ae. aegypti.

scholarly journals Localization and role of inward rectifier K+ channels in Malpighian tubules of the yellow fever mosquito Aedes aegypti

2015 ◽  
Vol 67 ◽  
pp. 59-73 ◽  
Author(s):  
Peter M. Piermarini ◽  
Sonja M. Dunemann ◽  
Matthew F. Rouhier ◽  
Travis L. Calkins ◽  
Rene Raphemot ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Yellow Fever ◽  
Inward Rectifier ◽  
Malpighian Tubules ◽  
Yellow Fever Mosquito ◽  
K Channels

The control of the diuresis following a blood meal in females of the yellow fever mosquito Aedes aegypti (L)

1977 ◽  
Vol 69 (1) ◽  
pp. 53-85
Author(s):  
R. H. Stobbart
Keyword(s):  
Aedes Aegypti ◽  
Yellow Fever ◽  
Surgical Procedures ◽  
Blood Meal ◽  
Sensory Information ◽  
Feedback Mechanism ◽  
Malpighian Tubules ◽  
Diuretic Hormone ◽  
Post Feeding ◽  
Stomatogastric System

Control of post-feeding diuresis in females of the mosquito Aedes aegypti has been studied by means of a weighing technique and simple surgical procedures. The primary controlling factor is (as in the larva) a nervous feedback mechanism and not an increased production of diuretic hormone. As the mosquito ingests blood, sensory information from the distending abdomen reaches the mid gut via the nerve cord, brain and stomatogastric system. This information probably inhibits retroperistaltic movements of the mid gut allowing fluid from the Malpighian tubules (which seems to be produced continually) to be moved back to the rectum for subsequent excretion, instead of being re-cycled to the haemolymph via the mid gut. Such a control does not preclude a role for the diuretic hormone, continual secretion of which may cause the continual production of fluid from the Malpighian tubules.

scholarly journals NHE8 is an intracellular cation/H+ exchanger in renal tubules of the yellow fever mosquito Aedes aegypti

2009 ◽  
Vol 296 (4) ◽  
pp. F730-F750 ◽  
Author(s):  
Peter M. Piermarini ◽  
Dirk Weihrauch ◽  
Heiko Meyer ◽  
Markus Huss ◽  
Klaus W. Beyenbach
Keyword(s):  
Aedes Aegypti ◽  
Yellow Fever ◽  
Xenopus Oocytes ◽  
Intracellular Localization ◽  
Malpighian Tubules ◽  
Xenopus Laevis Oocytes ◽  
Renal Tubules ◽  
Yellow Fever Mosquito ◽  
Western Blots ◽  
Principal Cells

The goal of this study was to identify and characterize the hypothesized apical cation/H+ exchanger responsible for K+ and/or Na+ secretion in the renal (Malpighian) tubules of the yellow fever mosquito Aedes aegypti. From Aedes Malpighian tubules, we cloned “ AeNHE8,” a full-length cDNA encoding an ortholog of mammalian Na+/H+ exchanger 8 (NHE8). The expression of AeNHE8 transcripts is ubiquitous among mosquito tissues and is not enriched in Malpighian tubules. Western blots of Malpighian tubules suggest that AeNHE8 is expressed primarily as an intracellular protein, which was confirmed by immunohistochemical localizations in Malpighian tubules. AeNHE8 immunoreactivity is expressed in principal cells of the secretory, distal segments, where it localizes to a subapical compartment (e.g., vesicles or endosomes), but not in the apical brush border. Furthermore, feeding mosquitoes a blood meal or treating isolated tubules with dibutyryl-cAMP, both of which stimulate a natriuresis by Malpighian tubules, do not influence the intracellular localization of AeNHE8 in principal cells. When expressed heterologously in Xenopus laevis oocytes, AeNHE8 mediates EIPA-sensitive Na/H exchange, in which Li+ partially and K+ poorly replace Na+. The expression of AeNHE8 in Xenopus oocytes is associated with the development of a conductive pathway that closely resembles the known endogenous nonselective cation conductances of Xenopus oocytes. In conclusion, AeNHE8 does not mediate cation/H+ exchange in the apical membrane of Aedes Malpighian tubules; it is more likely involved with an intracellular function.

Effects of amiloride on isolated Malpighian tubules of the yellow fever mosquito (Aedes aegypti)

1992 ◽  
Vol 38 (5) ◽  
pp. 329-337 ◽  
Author(s):  
J.L. Hegarty ◽  
B. Zhang ◽  
M.C. Carroll ◽  
E.J. Cragoe ◽  
K.W. Beyenbach
Keyword(s):  
Aedes Aegypti ◽  
Yellow Fever ◽  
Malpighian Tubules ◽  
Yellow Fever Mosquito
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