scholarly journals Functional enhancement of chitosan and nanoparticles in cell culture, tissue engineering, and pharmaceutical applications

2012 ◽  
Vol 3 ◽  
Author(s):  
Wenjuan Gao ◽  
James C. K. Lai ◽  
Solomon W. Leung
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Pharmaceutical Applications

scholarly journals Decellularized Porcine Brain Matrix for Cell Culture and Tissue Engineering Scaffolds

2011 ◽  
Vol 17 (21-22) ◽  
pp. 2583-2592 ◽  
Author(s):  
Jessica A. DeQuach ◽  
Shauna H. Yuan ◽  
Lawrence S.B. Goldstein ◽  
Karen L. Christman
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Tissue Engineering Scaffolds ◽  
Porcine Brain

A Honeycomb Collagen Carrier for Cell Culture as a Tissue Engineering Scaffold

2001 ◽  
Vol 25 (3) ◽  
pp. 213-217 ◽  
Author(s):  
Hiroshi Itoh ◽  
Yu Aso ◽  
Masayasu Furuse ◽  
Yasuharu Noishiki ◽  
Teruo Miyata
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Tissue Engineering Scaffold

scholarly journals Binary Biocompatible CNC–Gelatine Hydrogel as 3D Scaffolds Suitable for Cell Culture Adhesion and Growth

Applied Nano ◽  
2021 ◽  
Vol 2 (2) ◽  
pp. 118-127
Author(s):  
Luca Zoia ◽  
Anna Binda ◽  
Laura Cipolla ◽  
Ilaria Rivolta ◽  
Barbara La Ferla
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Cell Cultures ◽  
Cellulose Nanocrystals ◽  
Potential Application ◽  
Chemical Properties ◽  
Cellular Adhesion ◽  
Chemical Crosslinking ◽  
3D Scaffolds ◽  
Physical Chemical

Binary nano-biocomposite 3D scaffolds of cellulose nanocrystals (CNCs)—gelatine were fabricated without using chemical crosslinking additives. Controlled oxidative treatment allowed introducing carboxyl or carbonyl functionalities on the surface of CNCs responsible for the crosslinking of gelatine polymers. The obtained composites were characterized for their physical-chemical properties. Their biocompatibility towards different cell cultures was evaluated through MTT and LDH assays, cellular adhesion and proliferation experiments. Gelatine composites reinforced with carbonyl-modified CNCs showed the most performing swelling/degradation profile and the most promising adhesion and proliferation properties towards cell lines, suggesting their potential application in the field of tissue engineering.

scholarly journals Design and Biophysical Characterization of Poly (l-Lactic) Acid Microcarriers with and without Modification of Chitosan and Nanohydroxyapatite

Polymers ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 1061 ◽  
Author(s):  
Liying Li ◽  
Kedong Song ◽  
Yongzhi Chen ◽  
Yiwei Wang ◽  
Fangxin Shi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Lactic Acid ◽  
Average Diameter ◽  
Natural Polymers ◽  
Promoting Effect ◽  
Biophysical Characterization ◽  
Promising Tool ◽  
Cell Carrier

Nowadays, microcarriers are widely utilized in drug delivery, defect filling, and cell culture. Also, many researchers focus on the combination of synthetic and natural polymers and bioactive ceramics to prepare composite biomaterials for tissue engineering and regeneration. In this study, three kinds of microcarriers were prepared based on physical doping and surface modification, named Poly (l-lactic) acid (PLLA), PLLA/nanohydroxyapatite (PLLA/nHA), and PLLA/nHA/Chitosan (PLLA/nHA/Ch). The physicochemical properties of the microcarriers and their functional performances in MC3T3-E1 cell culture were compared. Statistical results showed that the average diameter of PLLA microcarriers was 291.9 ± 30.7 μm, and that of PLLA/nHA and PLLA/nHA/Ch microcarriers decreased to 275.7 ± 30.6 μm and 269.4 ± 26.3 μm, respectively. The surface roughness and protein adsorption of microcarriers were enhanced with the doping of nHA and coating of chitosan. The cell-carrier cultivation stated that the PLLA/nHA microcarriers had the greatest proliferation-promoting effect, while the PLLA/nHA/Ch microcarriers performed the strongest attachment with MC3T3-E1 cells. Besides, the cells on the PLLA/nHA/Ch microcarriers exhibited optimal osteogenic expression. Generally, chitosan was found to improve microcarriers with superior characteristics in cell adhesion and differentiation, and nanohydroxyapatite was beneficial for microcarriers regarding sphericity and cell proliferation. Overall, the modified microcarriers may be considered as a promising tool for bone tissue engineering.

scholarly journals Recent Advances in Application of Biosensors in Tissue Engineering

2014 ◽  
Vol 2014 ◽  
pp. 1-18 ◽  
Author(s):  
Anwarul Hasan ◽  
Md Nurunnabi ◽  
Mahboob Morshed ◽  
Arghya Paul ◽  
Alessandro Polini ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Small Molecules ◽  
Real Time ◽  
Inflammatory Cytokines ◽  
Serum Proteins ◽  
Molecular Size ◽  
Medical Diagnostics ◽  
Alpha Fetoprotein ◽  
Pharmaceutical Industries

Biosensors research is a fast growing field in which tens of thousands of papers have been published over the years, and the industry is now worth billions of dollars. The biosensor products have found their applications in numerous industries including food and beverages, agricultural, environmental, medical diagnostics, and pharmaceutical industries and many more. Even though numerous biosensors have been developed for detection of proteins, peptides, enzymes, and numerous other biomolecules for diverse applications, their applications in tissue engineering have remained limited. In recent years, there has been a growing interest in application of novel biosensors in cell culture and tissue engineering, for example, real-time detection of small molecules such as glucose, lactose, and H2O2as well as serum proteins of large molecular size, such as albumin and alpha-fetoprotein, and inflammatory cytokines, such as IFN-g and TNF-α. In this review, we provide an overview of the recent advancements in biosensors for tissue engineering applications.

Control of cell growth on 3D-printed cell culture platforms for tissue engineering

2017 ◽  
Vol 105 (12) ◽  
pp. 3281-3292 ◽  
Author(s):  
Zhikai Tan ◽  
Tong Liu ◽  
Juchang Zhong ◽  
Yikun Yang ◽  
Weihong Tan
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Cell Growth ◽  
3D Printed

scholarly journals Methods of Modification of Mesenchymal Stem Cells and Conditions of Their Culturing for Hyaline Cartilage Tissue Engineering

Biomedicines ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 1666
Author(s):  
Maria V. Shestovskaya ◽  
Svetlana A. Bozhkova ◽  
Julia V. Sopova ◽  
Mikhail G. Khotin ◽  
Mikhail S. Bozhokin
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Clinical Practice ◽  
Regenerative Medicine ◽  
Hyaline Cartilage ◽  
Cartilage Tissue Engineering ◽  
Matrix Proteins ◽  
Cartilage Tissue ◽  
Biodegradable Scaffolds ◽  
Cartilage Extracellular Matrix

The use of mesenchymal stromal cells (MSCs) for tissue engineering of hyaline cartilage is a topical area of regenerative medicine that has already entered clinical practice. The key stage of this procedure is to create conditions for chondrogenic differentiation of MSCs, increase the synthesis of hyaline cartilage extracellular matrix proteins by these cells and activate their proliferation. The first such works consisted in the indirect modification of cells, namely, in changing the conditions in which they are located, including microfracturing of the subchondral bone and the use of 3D biodegradable scaffolds. The most effective methods for modifying the cell culture of MSCs are protein and physical, which have already been partially introduced into clinical practice. Genetic methods for modifying MSCs, despite their effectiveness, have significant limitations. Techniques have not yet been developed that allow studying the effectiveness of their application even in limited groups of patients. The use of MSC modification methods allows precise regulation of cell culture proliferation, and in combination with the use of a 3D biodegradable scaffold, it allows obtaining a hyaline-like regenerate in the damaged area. This review is devoted to the consideration and comparison of various methods used to modify the cell culture of MSCs for their use in regenerative medicine of cartilage tissue.

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