scholarly journals Aliskiren Improves Ischemia- and Oxygen Glucose Deprivation-Induced Cardiac Injury through Activation of Autophagy and AMP-Activated Protein Kinase

2017 ◽  
Vol 8 ◽  
Author(s):  
Ming-Hsien Chiang ◽  
Chan-Jung Liang ◽  
Chen-Wei Liu ◽  
Bo-Jhih Pan ◽  
Wen-Ping Chen ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Cardiac Injury ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Amp Activated Protein Kinase

AMP-activated protein kinase is involved in induction of protective autophagy in astrocytes exposed to oxygen-glucose deprivation

2014 ◽  
Vol 38 (10) ◽  
pp. 1086-1097 ◽  
Author(s):  
Bożena Gabryel ◽  
Alicja Kost ◽  
Daniela Kasprowska ◽  
Sebastian Liber ◽  
Grzegorz Machnik ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Amp Activated Protein Kinase

scholarly journals AMP-activated protein kinase is involved in induction of protective autophagy in astrocytes exposed to oxygen-glucose deprivation

2017 ◽  
Vol 41 (8) ◽  
pp. 928-931 ◽  
Author(s):  
Bożena Gabryel ◽  
Alicja Kost ◽  
Daniela Kasprowska ◽  
Sebastian Liber ◽  
Grzegorz Machnik ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Amp Activated Protein Kinase

Identification and characterization of a novel sucrose-non-fermenting protein kinase/AMP-activated protein kinase-related protein kinase, SNARK

2001 ◽  
Vol 355 (2) ◽  
pp. 297-305 ◽  
Author(s):  
Diana L. LEFEBVRE ◽  
Yahong BAI ◽  
Nazanin SHAHMOLKY ◽  
Monika SHARMA ◽  
Raymond POON ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Cellular Response ◽  
Catalytic Domain ◽  
Metabolic Stress ◽  
Glucose Deprivation ◽  
Protein Band ◽  
Northern Blotting ◽  
Significant Similarity ◽  
Amp Activated Protein Kinase

Subtraction hybridization after the exposure of keratinocytes to ultraviolet radiation identified a differentially expressed cDNA that encodes a protein of 630 amino acid residues possessing significant similarity to the catalytic domain of the sucrose-non-fermenting protein kinase (SNF1)/AMP-activated protein kinase (AMPK) family of serine/threonine protein kinases. Northern blotting and reverse-transcriptase-mediated PCR demonstrated that mRNA transcripts for the SNF1/AMPK-related kinase (SNARK) were widely expressed in rodent tissues. The SNARK gene was localized to human chromosome 1q32 by fluorescent in situ hybridization. SNARK was translated in vitro to yield a single protein band of approx. 76kDa; Western analysis of transfected baby hamster kidney (BHK) cells detected two SNARK-immunoreactive bands of approx. 76-80kDa. SNARK was capable of autophosphorylation in vitro; immunoprecipitated SNARK exhibited phosphotransferase activity with the synthetic peptide substrate HMRSAMSGLHLVKRR (SAMS) as a kinase substrate. SNARK activity was significantly increased by AMP and 5-amino-4-imidazolecarboxamide riboside (AICAriboside) in rat keratinocyte cells, implying that SNARK might be activated by an AMPK kinase-dependent pathway. Furthermore, glucose deprivation increased SNARK activity 3-fold in BHK fibroblasts. These findings identify SNARK as a glucose- and AICAriboside-regulated member of the AMPK-related gene family that represents a new candidate mediator of the cellular response to metabolic stress.

Isoflurane Preconditions Hippocampal Neurons against Oxygen–Glucose Deprivation

2005 ◽  
Vol 103 (3) ◽  
pp. 532-539 ◽  
Author(s):  
Philip E. Bickler ◽  
Xinhua Zhan ◽  
Christian S. Fahlman
Keyword(s):  
Endoplasmic Reticulum ◽  
Protein Kinase ◽  
Hippocampal Slice ◽  
Binding Protein ◽  
Glucose Deprivation ◽  
Mitogen Activated Protein Kinase ◽  
Oxygen Glucose Deprivation ◽  
Hippocampal Slice Cultures ◽  
Mitogen Activated Protein ◽  
Associated Proteins

Background Isoflurane preconditions neurons to improve tolerance of subsequent ischemia in both intact animal models and in in vitro preparations. The mechanisms for this protection remain largely undefined. Because isoflurane increases intracellular Ca2+ concentrations and Ca2+ is involved in many processes related to preconditioning, the authors hypothesized that isoflurane preconditions neurons via Ca2+-dependent processes involving the Ca2+- binding protein calmodulin and the mitogen-activated protein kinase-ERK pathway. Methods The authors used a preconditioning model in which organotypic cultures of rat hippocampus were exposed to 0.5-1.5% isoflurane for a 2-h period 24 h before an ischemia-like injury of oxygen-glucose deprivation. Survival of CA1, CA3, and dentate neurons was assessed 48 later, along with interval measurements of intracellular Ca2+ concentration (fura-2 fluorescence microscopy in CA1 neurons), mitogen-activated protein kinase p42/44, and the survival associated proteins Akt and GSK-3beta (in situ immunostaining and Western blots). Results Preconditioning with 0.5-1.5% isoflurane decreased neuron death in CA1 and CA3 regions of hippocampal slice cultures after oxygen-glucose deprivation. The preconditioning period was associated with an increase in basal intracellular Ca2+ concentration of 7-15%, which involved Ca2+ release from inositol triphosphate-sensitive stores in the endoplasmic reticulum, and transient phosphorylation of mitogen-activated protein kinase p42/44 and the survival-associated proteins Akt and GSK-3beta. Preconditioning protection was eliminated by the mitogen-activated extracellular kinase inhibitor U0126, which prevented phosphorylation of p44 during preconditioning, and by calmidazolium, which antagonizes the effects of Ca2+-bound calmodulin. Conclusions Isoflurane, at clinical concentrations, preconditions neurons in hippocampal slice cultures by mechanisms that apparently involve release of Ca2+ from the endoplasmic reticulum, transient increases in intracellular Ca2+ concentration, the Ca2+ binding protein calmodulin, and phosphorylation of the mitogen-activated protein kinase p42/44.

scholarly journals A role of DNA-dependent protein kinase for the activation of AMP-activated protein kinase in response to glucose deprivation

2012 ◽  
Vol 1823 (12) ◽  
pp. 2099-2108 ◽  
Author(s):  
Parmeshwar Narayan Amatya ◽  
Hong-Beum Kim ◽  
Seon-Joo Park ◽  
Cha-Kyung Youn ◽  
Jin-Won Hyun ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Glucose Deprivation ◽  
Dependent Protein Kinase ◽  
Dependent Protein ◽  
Amp Activated Protein Kinase
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