scholarly journals Silymarin Protects Mouse Liver and Kidney from Thioacetamide Induced Toxicity by Scavenging Reactive Oxygen Species and Activating PI3K-Akt Pathway

2016 ◽  
Vol 7 ◽  
Author(s):  
Shatadal Ghosh ◽  
Abhijit Sarkar ◽  
Sudip Bhattacharyya ◽  
Parames C. Sil
Keyword(s):  
Reactive Oxygen Species ◽  
Mouse Liver ◽  
Reactive Oxygen ◽  
Akt Pathway ◽  
Oxygen Species ◽  
Liver And Kidney

scholarly journals Involvement of Reactive Oxygen Species in the Hepatorenal Toxicity of Actinomycin V In Vitro and In Vivo

Marine Drugs ◽  
2020 ◽  
Vol 18 (8) ◽  
pp. 428
Author(s):  
Fu-juan Jia ◽  
Zhuo Han ◽  
Jia-hui Ma ◽  
Shi-qing Jiang ◽  
Xing-ming Zhao ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Therapeutic Agent ◽  
Reactive Oxygen ◽  
Normal Liver ◽  
Oxygen Species ◽  
First Line ◽  
Liver And Kidney ◽  
Further Development

The high toxicity of actinomycin D (Act D) severely limits its use as a first-line chemotherapeutic agent in the clinic. Actinomycin V (Act V), an analog of Act D, exhibited strong anticancer activity in our previous studies. Here, we provide evidence that Act V has less hepatorenal toxicity than Act D in vitro and in vivo, associated with the reactive oxygen species (ROS) pathway. Compared to Act D, Act V exhibited considerably stronger sensitivity for cancer cells and less toxicity to human normal liver LO-2 and human embryonic kidney 293T cells using the MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) assay. Notably, Act V caused less damage to both the liver and kidney than Act D in vivo, indicated by organ to body weight ratios, as well as alanine aminotransferase (ALT), aspartate aminotransferase (AST), and serum creatinine (Scr) levels. Further experiments showed that the ROS pathway is involved in Act V-induced hepatorenal toxicity. Act V generates ROS and accumulates malondialdehyde (MDA), reducing levels of superoxide dismutase (SOD) and glutathione (GSH) in LO-2 and 293T cells. These findings indicate that Act V induces less hepatorenal toxicity than Act D in vitro and in vivo and merits further development as a potential therapeutic agent for the treatment of cancer.

Copper Ions Strongly Activate the Phosphoinositide-3-Kinase/Akt Pathway Independent of the Generation of Reactive Oxygen Species

2002 ◽  
Vol 397 (2) ◽  
pp. 232-239 ◽  
Author(s):  
Elena A. Ostrakhovitch ◽  
Mohammad Reza Lordnejad ◽  
Freimut Schliess ◽  
Helmut Sies ◽  
Lars-Oliver Klotz
Keyword(s):  
Reactive Oxygen Species ◽  
Copper Ions ◽  
Reactive Oxygen ◽  
Akt Pathway ◽  
Oxygen Species ◽  
Phosphoinositide 3 Kinase
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