scholarly journals Carbohydrate-Mediated Modulation of NK Cell Receptor Function: Structural and Functional Influences of Heparan Sulfate Moieties Expressed on NK Cell Surface

2014 ◽  
Vol 4 ◽  
Author(s):  
Michael Brusilovsky ◽  
Olga Radinsky ◽  
Rami Yossef ◽  
Kerry S. Campbell ◽  
Angel Porgador
Keyword(s):  
Cell Surface ◽  
Heparan Sulfate ◽  
Nk Cell ◽  
Cell Receptor ◽  
Receptor Function ◽  
Nk Cell Receptor

scholarly journals Pseudorabies Virus Infection Causes Downregulation of Ligands for the Activating NK Cell Receptor NKG2D

Viruses ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 266
Author(s):  
Sofie Denaeghel ◽  
Steffi De Pelsmaeker ◽  
Cliff Van Waesberghe ◽  
Herman W. Favoreel
Keyword(s):  
Cell Surface ◽  
Nk Cells ◽  
Pseudorabies Virus ◽  
Nk Cell ◽  
Cell Receptor ◽  
Host Cells ◽  
Nkg2d Ligand ◽  
Antiviral Immune Response ◽  
Nkg2d Ligands ◽  
Nk Cell Receptor

Herpesviruses display a complex and carefully balanced interaction with important players in the antiviral immune response of immunocompetent natural hosts, including natural killer (NK) cells. With regard to NK cells, this delicate balance is illustrated on the one hand by severe herpesvirus disease reported in individuals with NK cell deficiencies and on the other hand by several NK cell evasion strategies described for herpesviruses. In the current study, we report that porcine cells infected with the porcine alphaherpesvirus pseudorabies virus (PRV) display a rapid and progressive downregulation of ligands for the major activating NK cell receptor NKG2D. This downregulation consists both of a downregulation of NKG2D ligands that are already expressed on the cell surface of an infected cell and an inhibition of cell surface expression of newly expressed NKG2D ligands. Flow cytometry and RT-qPCR assays showed that PRV infection results in downregulation of the porcine NKG2D ligand pULBP1 from the cell surface and a very substantial suppression of mRNA expression of pULBP1 and of another potential NKG2D ligand, pMIC2. Furthermore, PRV-induced NKG2D ligand downregulation was found to be independent of late viral gene expression. In conclusion, we report that PRV infection of host cells results in a very pronounced downregulation of ligands for the activating NK cell receptor NKG2D, representing an additional NK evasion strategy of PRV.

scholarly journals Genome-Wide siRNA Screen Reveals a New Cellular Partner of NK Cell Receptor KIR2DL4: Heparan Sulfate Directly Modulates KIR2DL4-Mediated Responses

2013 ◽  
Vol 191 (10) ◽  
pp. 5256-5267 ◽  
Author(s):  
Michael Brusilovsky ◽  
Moti Cordoba ◽  
Benyamin Rosental ◽  
Oren Hershkovitz ◽  
Mark D. Andrake ◽  
...  
Keyword(s):  
Heparan Sulfate ◽  
Nk Cell ◽  
Cell Receptor ◽  
Sirna Screen ◽  
Genome Wide ◽  
Cellular Partner ◽  
Nk Cell Receptor

scholarly journals Activating NK cell receptor ligands are differentially expressed during progression to cervical cancer

2008 ◽  
Vol 123 (10) ◽  
pp. 2343-2353 ◽  
Author(s):  
Sonja Textor ◽  
Matthias Dürst ◽  
Lars Jansen ◽  
Rosita Accardi ◽  
Massimo Tommasino ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Nk Cell ◽  
Cell Receptor ◽  
Differentially Expressed ◽  
Receptor Ligands ◽  
Nk Cell Receptor

scholarly journals Functionally and Structurally Distinct NK Cell Receptor Repertoires in the Peripheral Blood of Two Human Donors

Immunity ◽  
1997 ◽  
Vol 7 (6) ◽  
pp. 739-751 ◽  
Author(s):  
Nicholas M Valiante ◽  
Markus Uhrberg ◽  
Heather G Shilling ◽  
Kristin Lienert-Weidenbach ◽  
Kelly L Arnett ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Nk Cell ◽  
Cell Receptor ◽  
Nk Cell Receptor

KLRL1, a novel killer cell lectinlike receptor, inhibits natural killer cell cytotoxicity

Blood ◽  
2004 ◽  
Vol 104 (9) ◽  
pp. 2858-2866 ◽  
Author(s):  
Yanmei Han ◽  
Minghui Zhang ◽  
Nan Li ◽  
Taoyong Chen ◽  
Yi Zhang ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Natural Killer ◽  
Nk Cell ◽  
Killer Cell ◽  
Cell Receptor ◽  
Sh2 Domain ◽  
Target Cells ◽  
Lymphoid Tissues ◽  
Nk Cell Receptor ◽  
Human And Mouse

Abstract Natural killer (NK) cell inhibitory receptors play important roles in the regulation of target susceptibility to natural killing. Here, we report the molecular cloning and functional characterization of a novel NK cell receptor, KLRL1, from human and mouse dendritic cells. KLRL1 is a type II transmembrane protein with an immunoreceptor tyrosine-based inhibitory motif and a C-type lectinlike domain. The KLRL1 gene is located in the central region of the NK gene complex in both humans and mice, on human chromosome 12p13 and mouse chromosome 6F3, adjacent to the other KLR genes. KLRL1 is preferentially expressed in lymphoid tissues and immune cells, including NK cells, T cells, dendritic cells, and monocytes or macrophages. Western blot and fluorescence confocal microscopy analyses indicated that KLRL1 is a membrane-associated glycoprotein, which forms a heterodimer with an as yet unidentified partner. Human and mouse KLRL1 are both predicted to contain putative immunoreceptor tyrosine-based inhibitory motifs (ITIMs), and immunoprecipitation experiments demonstrated that KLRL1 associates with the tyrosine phosphatases SHP-1 (SH2-domain-containing protein tyrosine phosphatase 1) and SHP-2. Consistent with its potential inhibitory function, pretreatment of target cells with human KLRL1-Fc fusion protein enhances NK-mediated cytotoxicity. Taken together, our results demonstrate that KLRL1 belongs to the KLR family and is a novel inhibitory NK cell receptor.

scholarly journals Back signaling of HLA class I molecules and T/ NK cell receptor ligands in epithelial cells reflects the rejection‐specific microenvironment in renal allograft biopsies

2019 ◽  
Vol 19 (10) ◽  
pp. 2692-2704 ◽  
Author(s):  
Johanna Egelkamp ◽  
Evgeny Chichelnitskiy ◽  
Jenny F. Kühne ◽  
Franziska Wandrer ◽  
Kerstin Daemen ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Renal Allograft ◽  
Nk Cell ◽  
Hla Class I ◽  
Cell Receptor ◽  
Class I ◽  
Receptor Ligands ◽  
Hla Class I Molecules ◽  
Nk Cell Receptor

scholarly journals Deletion of the activating NK cell receptor NKG2D accelerates rejection of cardiac allografts

2017 ◽  
Vol 17 (12) ◽  
pp. 3199-3209 ◽  
Author(s):  
Cornelia Fabritius ◽  
Paul Viktor Ritschl ◽  
Thomas Resch ◽  
Mario Roth ◽  
Susanne Ebner ◽  
...  
Keyword(s):  
Nk Cell ◽  
Cell Receptor ◽  
Cardiac Allografts ◽  
Nk Cell Receptor
Sign in / Sign up

Export Citation Format

Share Document