scholarly journals Intraocular Injection of ES Cell-Derived Neural Progenitors Improve Visual Function in Retinal Ganglion Cell-Depleted Mouse Models

2017 ◽  
Vol 11 ◽  
Author(s):  
Mundackal S. Divya ◽  
Vazhanthodi A. Rasheed ◽  
Tiffany Schmidt ◽  
Soundararajan Lalitha ◽  
Samer Hattar ◽  
...  
Keyword(s):  
Ganglion Cell ◽  
Retinal Ganglion Cell ◽  
Mouse Models ◽  
Visual Function ◽  
Neural Progenitors ◽  
Retinal Ganglion ◽  
Es Cell ◽  
Intraocular Injection

scholarly journals Intraocular injection of ES cell-derived neural progenitors improve visual function in retinal ganglion cell-depleted mouse models

2017 ◽  
Author(s):  
Mundackal Sivaraman Divya ◽  
Vazhanthodi Abdul Rasheed ◽  
Tiffany Schmidt ◽  
Soundararajan Lalitha ◽  
Samer Hattar ◽  
...  
Keyword(s):  
Visual Acuity ◽  
Ganglion Cell ◽  
Retinal Ganglion Cell ◽  
Mouse Models ◽  
Visual Function ◽  
Functional Integration ◽  
Embryonic Stem ◽  
Neural Progenitors ◽  
Retinal Ganglion ◽  
Es Cell

AbstractRetinal ganglion cells (RGC) transplantation is a promising strategy to restore visual function resulting from irreversible RGC degeneration occurring in glaucoma or inherited optic neuropathies. We previously demonstrated FGF2 induced differentiation of mouse embryonic stem cells (ESC) to RGC lineage, capable of retinal Ganglion Cell Layer (GCL) integration upon transplantation in mice. Here, we evaluated possible improvement of visual function by transplantation of ES cell derived neural progenitors in RGC depleted glaucoma mice models. ESC derived neural progenitors (ES-NP) were transplanted into NMDA (N-Methyl-D-Aspartate) injected, RGC-ablated mouse models and a pre-clinical glaucoma mouse model (DBA/2J) having sustained higher intra ocular pressure (IOP). Visual acuity and functional integration was evaluated by behavioural experiments and immunohistochemistry, respectively. GFP-expressing ES-NPs transplanted in NMDA-injected RGC-depleted mice differentiated into RGC lineage and possibly integrating into GCL. An improvement in visual acuity was observed after two months of transplantation, when compared to the pre-transplantation values. Expression of c-Fos in the transplanted cells, upon light induction, further suggests functional integration into the host retinal circuitry. However, the transplanted cells did not send axonal projections into optic nerve. Transplantation experiments in DBA/2J mouse showed no significant improvement in visual functions, possibly due to both host and transplanted retinal cell death which could be due to an inherent high IOP. We showed that, transplantation of ES-NPs into the retina of RGC-ablated mouse models could survive, differentiate to RGC lineage, and possibly integrate into GCL to improve visual function. However, for the survival of transplanted cells in glaucoma, strategies to control the IOP are warranted.

Retinal ganglion cell layer and visual function in a patient with optic disc drusen

1998 ◽  
Vol 236 (12) ◽  
pp. 904-915 ◽  
Author(s):  
M.-M. Gellrich ◽  
Stephan Neumaier ◽  
Claudia Auw-Hädrich ◽  
Nils-Claudius Gellrich ◽  
Lutz Lothar Hansen
Keyword(s):  
Ganglion Cell ◽  
Optic Disc ◽  
Retinal Ganglion Cell ◽  
Visual Function ◽  
Ganglion Cell Layer ◽  
Cell Layer ◽  
Retinal Ganglion ◽  
Optic Disc Drusen ◽  
Retinal Ganglion Cell Layer

scholarly journals The Relationship Between Macula Retinal Ganglion Cell Density and Visual Function in the Nonhuman Primate

2021 ◽  
Vol 62 (1) ◽  
pp. 5
Author(s):  
Kwame Antwi-Boasiako ◽  
Louvenia Carter-Dawson ◽  
Ronald Harwerth ◽  
Margaret Gondo ◽  
Nimesh Patel
Keyword(s):  
Ganglion Cell ◽  
Cell Density ◽  
Retinal Ganglion Cell ◽  
Nonhuman Primate ◽  
Visual Function ◽  
Retinal Ganglion ◽  
The Relationship

scholarly journals Effect of Ubiquinol on Glaucomatous Neurodegeneration and Oxidative Stress: Studies for Retinal Ganglion Cell Survival and/or Visual Function

Antioxidants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 952
Author(s):  
Genea Edwards ◽  
Yonghoon Lee ◽  
Martha Kim ◽  
Soham Bhanvadia ◽  
Keun-Young Kim ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ganglion Cell ◽  
Retinal Ganglion Cell ◽  
Visual Function ◽  
Therapeutic Potential ◽  
Control Diet ◽  
Retinal Ganglion ◽  
Complex Ii ◽  
Oxphos Complex ◽  
And Oxidative Stress

Oxidative stress is one of major causal factors in glaucomatous neurodegeneration. Ubiquinol promotes retinal ganglion cell (RGC) survival against glaucomatous insults such as oxidative stress. Here we investigated the effect of ubiquinol on RGC survival and/or visual function in mouse models of glaucoma and oxidative stress. DBA/2J and age-matched DBA/2J-Gpnmb+ (D2-Gpnmb+), which do not develop intraocular pressure elevation, or C57BL/6J mice were fed with ubiquinol (1%) or control diet daily for 5 or 2 months. We assessed RGC survival by Brn3a immunohistochemistry and measured expression levels of active and total BAX, peroxisome proliferator-activated receptor-gamma coactivator 1α, transcription factor A (TFAM) and oxidative phosphorylation (OXPHOS) complex protein. Following induction of oxidative stress by paraquat injection, we also assessed visual function. In glaucomatous retina, ubiquinol supplementation significantly promoted RGC survival, blocked BAX activation and increased TFAM and OXPHOS complex II protein expression. Also, ubiquinol supplementation ameliorated oxidative stress-induced visual dysfunction. These findings indicate that ubiquinol promotes RGC survival by increasing TFAM expression and OXPHOS complex II activity in glaucomatous neurodegeneration, and that ubiquinol enhances RGC survival and preserves visual function against oxidative stress. We propose that ubiquinol has a therapeutic potential for treating oxidative stress-associated glaucomatous neurodegeneration.

scholarly journals Author Correction: Matrix-bound nanovesicles prevent ischemia-induced retinal ganglion cell axon degeneration and death and preserve visual function

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Yolandi van der Merwe ◽  
Anne E. Faust ◽  
Ecem T. Sakalli ◽  
Caroline C. Westrick ◽  
George Hussey ◽  
...  
Keyword(s):  
Ganglion Cell ◽  
Retinal Ganglion Cell ◽  
Visual Function ◽  
Axon Degeneration ◽  
Retinal Ganglion ◽  
Retinal Ganglion Cell Axon ◽  
Cell Axon ◽  
Matrix Bound ◽  
Ganglion Cell Axon

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

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