scholarly journals Cloning and Overexpression of the Toy Cluster for Titer Improvement of Toyocamycin in Streptomyces diastatochromogenes

2020 ◽  
Vol 11 ◽  
Author(s):  
Zheng Ma ◽  
Yefeng Hu ◽  
Zhijun Liao ◽  
Jie Xu ◽  
Xianhao Xu ◽  
...  
2010 ◽  
Vol 62 (3) ◽  
pp. 1062-1067 ◽  
Author(s):  
Guoliang Wang ◽  
Shiru Jia ◽  
Tian Wang ◽  
Liye Chen ◽  
Qingchao Song ◽  
...  

2013 ◽  
Vol 59 (2) ◽  
pp. 93-97 ◽  
Author(s):  
Zheng Ma ◽  
Jinxiu Liu ◽  
Xiaozhen Lin ◽  
Xuping Shentu ◽  
Yalin Bian ◽  
...  

2016 ◽  
Vol 29 (8) ◽  
pp. 640-650 ◽  
Author(s):  
Yucheng Zhang ◽  
Dawn R. D. Bignell ◽  
Ran Zuo ◽  
Qiurong Fan ◽  
Jose C. Huguet-Tapia ◽  
...  

Approximately 10 Streptomyces species cause disease on underground plant structures. The most economically important of these is potato scab, and the most studied of these pathogens is Streptomyces scabiei (syn. S. scabies). The main pathogenicity determinant of scab-causing Streptomyces species is a nitrated diketopiperazine, known as thaxtomin A (ThxA). In the pathogenic species Streptomyces turgidiscabies, ThxA biosynthetic genes reside on a mobile pathogenicity island (PAI). However, the mobilization of PAIs in other Streptomyces species remains uncharacterized. Here, we investigated the mobilization of the PAI of S. scabiei 87-22. Based on whole genome sequences, we inferred the evolutionary relationships of pathogenic Streptomyces species and discovered that Streptomyces sp. strain 96-12, a novel pathogenic species isolated from potatoes in Egypt, was phylogenetically grouped with nonpathogenic species rather than with known pathogenic species. We also found that Streptomyces sp. strain 96-12 contains a PAI that is almost identical to the PAI in S. scabiei 87-22, despite significant differences in their genome sequences. This suggested direct or indirect in vivo mobilization of the PAI between S. scabiei and nonpathogenic Streptomyces species. To test whether the S. scabiei 87-22 PAI could, indeed, be mobilized, S. scabiei 87-22 deletion mutants containing antibiotic resistance markers in the PAI were mated with Streptomyces diastatochromogenes, a nonpathogenic species. The PAI of S. scabiei was site-specifically inserted into the aviX1 gene of S. diastatochromogenes and conferred pathogenicity in radish seedling assays. Our results demonstrated that S. scabiei, the earliest described Streptomyces pathogen, could be the source of a PAI responsible for the emergence of novel pathogenic species.


2000 ◽  
Vol 53 (6) ◽  
pp. 575-578 ◽  
Author(s):  
YUYA YOSHIMOTO ◽  
TSUTOMU SAWA ◽  
HIROSHI NAGANAWA ◽  
TAKESHI SUGAI ◽  
TOMIO TAKEUCHI ◽  
...  

ChemInform ◽  
2010 ◽  
Vol 33 (3) ◽  
pp. no-no
Author(s):  
Ken-ichiro Hayashi ◽  
Kentaro Ogino ◽  
Yutaka Oono ◽  
Hirofumi Uchimiya ◽  
Hiroshi Nozaki

2015 ◽  
Vol 69 (5) ◽  
pp. 406-410 ◽  
Author(s):  
Xuping Shentu ◽  
Nannan Liu ◽  
Gu Tang ◽  
Yukinori Tanaka ◽  
Kozo Ochi ◽  
...  

1983 ◽  
Vol 47 (12) ◽  
pp. 2903-2905 ◽  
Author(s):  
Kazuaki HIGASHI ◽  
Isao KUSAKABE ◽  
Tsuneo YASUI ◽  
Tadayuki ISHIYAMA ◽  
Yoichiro OKIMOTO

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