Overexpression of ribosome recycling factor is responsible for improvement of nucleotide antibiotic-toyocamycin in Streptomyces diastatochromogenes 1628

2014 ◽  
Vol 98 (11) ◽  
pp. 5051-5058 ◽  
Author(s):  
Zheng Ma ◽  
Libin Tao ◽  
Andreas Bechthold ◽  
Xuping Shentu ◽  
Yalin Bian ◽  
...  
Keyword(s):  
Ribosome Recycling Factor ◽  
Streptomyces Diastatochromogenes

Thermal Stability of Thermoanaerobacter tengcongensis Ribosome Recycling Factor

2014 ◽  
Vol 21 (3) ◽  
pp. 285-291 ◽  
Author(s):  
Yi Shi ◽  
Dongyan Zheng ◽  
Jingyi Xie ◽  
Qijun Zhang ◽  
Hongjie Zhang
Keyword(s):  
Thermal Stability ◽  
Ribosome Recycling Factor ◽  
Thermoanaerobacter Tengcongensis ◽  
Thermal Stability Of

scholarly journals Specialized Metabolites from Ribosome Engineered Strains of Streptomyces clavuligerus

Metabolites ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 239
Author(s):  
Arshad Ali Shaikh ◽  
Louis-Felix Nothias ◽  
Santosh K. Srivastava ◽  
Pieter C. Dorrestein ◽  
Kapil Tahlan
Keyword(s):  
In Silico Analysis ◽  
Cost Effective ◽  
Gene Clusters ◽  
Streptomyces Clavuligerus ◽  
Strain Engineering ◽  
Ribosome Recycling Factor ◽  
Metabolic Potential ◽  
Putative Gene ◽  
Specialized Metabolites ◽  
Streptomyces Spp

Bacterial specialized metabolites are of immense importance because of their medicinal, industrial, and agricultural applications. Streptomyces clavuligerus is a known producer of such compounds; however, much of its metabolic potential remains unknown, as many associated biosynthetic gene clusters are silent or expressed at low levels. The overexpression of ribosome recycling factor (frr) and ribosome engineering (induced rpsL mutations) in other Streptomyces spp. has been reported to increase the production of known specialized metabolites. Therefore, we used an overexpression strategy in combination with untargeted metabolomics, molecular networking, and in silico analysis to annotate 28 metabolites in the current study, which have not been reported previously in S. clavuligerus. Many of the newly described metabolites are commonly found in plants, further alluding to the ability of S. clavuligerus to produce such compounds under specific conditions. In addition, the manipulation of frr and rpsL led to different metabolite production profiles in most cases. Known and putative gene clusters associated with the production of the observed compounds are also discussed. This work suggests that the combination of traditional strain engineering and recently developed metabolomics technologies together can provide rapid and cost-effective strategies to further speed up the discovery of novel natural products.

Effect of Ferrous Ion on ε-Poly-l-Lysine Biosynthesis by Streptomyces diastatochromogenes CGMCC3145

2010 ◽  
Vol 62 (3) ◽  
pp. 1062-1067 ◽  
Author(s):  
Guoliang Wang ◽  
Shiru Jia ◽  
Tian Wang ◽  
Liye Chen ◽  
Qingchao Song ◽  
...  
Keyword(s):  
Lysine Biosynthesis ◽  
Ferrous Ion ◽  
Streptomyces Diastatochromogenes

Formation, regeneration, and transformation of protoplasts of Streptomyces diastatochromogenes 1628

2013 ◽  
Vol 59 (2) ◽  
pp. 93-97 ◽  
Author(s):  
Zheng Ma ◽  
Jinxiu Liu ◽  
Xiaozhen Lin ◽  
Xuping Shentu ◽  
Yalin Bian ◽  
...  
Keyword(s):  
Streptomyces Diastatochromogenes

scholarly journals Cloning and Overexpression of the Toy Cluster for Titer Improvement of Toyocamycin in Streptomyces diastatochromogenes

2020 ◽  
Vol 11 ◽  
Author(s):  
Zheng Ma ◽  
Yefeng Hu ◽  
Zhijun Liao ◽  
Jie Xu ◽  
Xianhao Xu ◽  
...  
Keyword(s):  
Streptomyces Diastatochromogenes

scholarly journals Functional mapping of ribosome-contact sites in the ribosome recycling factor: A structural view from a tRNA mimic

RNA ◽  
2001 ◽  
Vol 7 (1) ◽  
pp. 64-70 ◽  
Author(s):  
TOSHINOBU FUJIWARA ◽  
KOICHI ITO ◽  
YOSHIKAZU NAKAMURA
Keyword(s):  
Functional Mapping ◽  
Ribosome Recycling Factor ◽  
Contact Sites

scholarly journals Complementary roles of initiation factor 1 and ribosome recycling factor in 70S ribosome splitting

2008 ◽  
Vol 27 (12) ◽  
pp. 1706-1717 ◽  
Author(s):  
Michael Y Pavlov ◽  
Ayman Antoun ◽  
Martin Lovmar ◽  
Måns Ehrenberg
Keyword(s):  
Initiation Factor ◽  
Ribosome Recycling Factor ◽  
70S Ribosome
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