scholarly journals Rescue of a Vaccinia Virus Mutant Lacking IFN Resistance Genes K1L and C7L by the Parapoxvirus Orf Virus

2020 ◽  
Vol 11 ◽  
Author(s):  
Sherief Riad ◽  
Yan Xiang ◽  
Basheer AlDaif ◽  
Andrew A. Mercer ◽  
Stephen B. Fleming
Keyword(s):  
Vaccinia Virus ◽  
Resistance Genes ◽  
Orf Virus ◽  
Virus Mutant

scholarly journals Molecular attenuation of vaccinia virus: mutant generation and animal characterization.

1992 ◽  
Vol 66 (5) ◽  
pp. 2617-2630 ◽  
Author(s):  
M S Lee ◽  
J M Roos ◽  
L C McGuigan ◽  
K A Smith ◽  
N Cormier ◽  
...  
Keyword(s):  
Vaccinia Virus ◽  
Virus Mutant

scholarly journals Variants of vaccinia virus hemagglutinin altered in intracellular transport.

1986 ◽  
Vol 6 (11) ◽  
pp. 3734-3745 ◽  
Author(s):  
H Shida
Keyword(s):  
Golgi Apparatus ◽  
Cell Surface ◽  
Vaccinia Virus ◽  
Intracellular Transport ◽  
Cytoplasmic Domain ◽  
Slow Movement ◽  
Wild Type ◽  
Sequence Analyses ◽  
In The Wild ◽  
Virus Mutant

Two classes of revertants were isolated from a vaccinia virus mutant whose hemagglutinins (HAs) accumulate on nuclear envelopes and rough endoplasmic reticulums. The HAs of one of the revertants had the same phenotype as the wild type, i.e., rapid and efficient movement to the cell surface. The HAs of the second class had biphasic transport: rapid export to the cell surface as in the wild type and slow movement to the medial cisternae of the Golgi apparatus. Biochemical and nucleotide sequence analyses showed that the HAs of all the mutants examined that have defects in transport from the rough endoplasmic reticulum to the Golgi apparatus have altered cytoplasmic domains and that the HAs of the second class of revertants lack the whole cytoplasmic domain, while the HAs of the first class of revertants have a wild-type cytoplasmic domain.

scholarly journals Characterization of a Vaccinia Virus Mutant with a Deletion of the D10R Gene Encoding a Putative Negative Regulator of Gene Expression

2006 ◽  
Vol 80 (2) ◽  
pp. 553-561 ◽  
Author(s):  
Susan Parrish ◽  
Bernard Moss
Keyword(s):  
Vaccinia Virus ◽  
Deletion Mutant ◽  
Negative Regulator ◽  
Wild Type Virus ◽  
Wild Type ◽  
Gene Encoding ◽  
Early Proteins ◽  
Late Transcription ◽  
Infected Cells ◽  
Virus Mutant

ABSTRACT The D9 and D10 proteins of vaccinia virus are 25% identical to each other, contain a mutT motif characteristic of nudix hydrolases, and are conserved in all sequenced poxviruses. Previous studies indicated that overexpression of D10 and, to a lesser extent, D9 decreased the levels of capped mRNAs and their translation products. Here, we further characterized the D10 protein and showed that only trace amounts are associated with purified virions and that it is expressed exclusively at late times after vaccinia virus infection. A viable deletion mutant (vΔD10) produced smaller plaques and lower virus yields than either wild-type virus or a D9R deletion mutant (vΔD9). Purified vΔD10 virions appeared normal by microscopic examination and biochemical analysis but produced 6- to 10-fold-fewer plaques at the same concentration as wild-type or vΔD9 virions. When 4 PFU per cell of wild-type or vΔD9 virions or equal numbers of vΔD10 virions were used for inoculation, nearly all cells were infected in each case, but viral early and late transcription was initiated more slowly in vΔD10-infected cells than in the others. However, viral early transcripts accumulated to higher levels in vΔD10-infected cells than in cells infected with the wild type or vΔD9. In addition, viral early and late mRNAs and cellular actin mRNA persisted longer in vΔD10-infected cells than in others. Furthermore, analysis of pulse-labeled proteins indicated prolonged synthesis of cellular and viral early proteins. These results are consistent with a role for D10 in regulating RNA levels in poxvirus-infected cells.

In vitro recognition of an orf virus early promoter in a vaccinia virus extract

1992 ◽  
Vol 123 (1-2) ◽  
pp. 223-228 ◽  
Author(s):  
J. C. Vos ◽  
A. A. Mercer ◽  
S. B. Fleming ◽  
A. J. Robinson
Keyword(s):  
Vaccinia Virus ◽  
Orf Virus ◽  
Early Promoter

Conservation of Gene Structure and Arrangement between Vaccinia Virus and Orf Virus

Virology ◽  
1993 ◽  
Vol 195 (1) ◽  
pp. 175-184 ◽  
Author(s):  
Stephen B. Fleming ◽  
Jan Blok ◽  
Kate M. Fraser ◽  
Andrew A. Mercer ◽  
Anthony J. Robinson
Keyword(s):  
Vaccinia Virus ◽  
Gene Structure ◽  
Orf Virus

Identification and Characterization of an Orf Virus Homologue of the Vaccinia Virus Gene Encoding the Major Envelope Antigen p37K

Virology ◽  
1994 ◽  
Vol 202 (2) ◽  
pp. 968-973 ◽  
Author(s):  
John T. Sullivan ◽  
Andrew A. Mercer ◽  
Stephen B. Fleming ◽  
Anthony J. Robinson
Keyword(s):  
Vaccinia Virus ◽  
Orf Virus ◽  
Gene Encoding ◽  
Virus Gene ◽  
Identification And Characterization ◽  
Envelope Antigen
Sign in / Sign up

Export Citation Format

Share Document