scholarly journals Fitness of Outer Membrane Vesicles From Komagataeibacter intermedius Is Altered Under the Impact of Simulated Mars-like Stressors Outside the International Space Station

2020 ◽  
Vol 11 ◽  
Author(s):  
Olga Podolich ◽  
Olga Kukharenko ◽  
Iryna Zaets ◽  
Iryna Orlovska ◽  
Larysa Palchykovska ◽  
...  
Keyword(s):  
Outer Membrane ◽  
International Space Station ◽  
Space Station ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
International Space ◽  
The Impact

scholarly journals Lipidomic Analysis of the Outer Membrane Vesicles from Paired Polymyxin-Susceptible and -Resistant Klebsiella pneumoniae Clinical Isolates

2018 ◽  
Vol 19 (8) ◽  
pp. 2356 ◽  
Author(s):  
Raad Jasim ◽  
Mei-Ling Han ◽  
Yan Zhu ◽  
Xiaohan Hu ◽  
Maytham Hussein ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Outer Membrane ◽  
Membrane Vesicles ◽  
Average Diameter ◽  
Polymyxin B ◽  
Outer Membrane Vesicles ◽  
Resistant Isolate ◽  
Average Particle ◽  
Lipid Species ◽  
The Impact

Gram-negative bacteria produce outer membrane vesicles (OMVs) as delivery vehicles for nefarious bacterial cargo such as virulence factors, which are antibiotic resistance determinants. This study aimed to investigate the impact of polymyxin B treatment on the OMV lipidome from paired polymyxin-susceptible and -resistant Klebsiella pneumoniae isolates. K. pneumoniae ATCC 700721 was employed as a reference strain in addition to two clinical strains, K. pneumoniae FADDI-KP069 and K. pneumoniae BM3. Polymyxin B treatment of the polymyxin-susceptible strains resulted in a marked reduction in the glycerophospholipid, fatty acid, lysoglycerophosphate and sphingolipid content of their OMVs. Conversely, the polymyxin-resistant strains expressed OMVs richer in all of these lipid species, both intrinsically and increasingly under polymyxin treatment. The average diameter of the OMVs derived from the K. pneumoniae ATCC 700721 polymyxin-susceptible isolate, measured by dynamic light scattering measurements, was ~90.6 nm, whereas the average diameter of the OMVs isolated from the paired polymyxin-resistant isolate was ~141 nm. Polymyxin B treatment (2 mg/L) of the K. pneumoniae ATCC 700721 cells resulted in the production of OMVs with a larger average particle size in both the susceptible (average diameter ~124 nm) and resistant (average diameter ~154 nm) strains. In light of the above, we hypothesize that outer membrane remodelling associated with polymyxin resistance in K. pneumoniae may involve fortifying the membrane structure with increased glycerophospholipids, fatty acids, lysoglycerophosphates and sphingolipids. Putatively, these changes serve to make the outer membrane and OMVs more impervious to polymyxin attack.

Status - International Space Station (ISS) Crewmembers' Noise Exposures

2021 ◽  
Vol 263 (4) ◽  
pp. 2740-2754
Author(s):  
Jose Limardo ◽  
Christopher S. Allen ◽  
Richard W. Danielson ◽  
Andrew J. Boone
Keyword(s):  
International Space Station ◽  
Noise Exposure ◽  
Space Station ◽  
Monitoring Program ◽  
Conservation Strategies ◽  
Exposure Limits ◽  
International Space ◽  
Exposure Monitoring ◽  
The Impact ◽  
Dosimetry Data

Environmental noise in space vehicles, caused by onboard equipment and crew activities, has generated concerns for crew health and safety since early U.S. space missions. The International Space Station (ISS) provides a unique environment where acoustic conditions can be monitored while crewmembers from the U.S. and their international partners work and live for as long as 6 to 12 consecutive months. This review of acoustic dosimetry data collected to date reveals that the noise exposure limits of NASA's stringent noise constraint flight rule have been exceeded in 41% of these dosimetry measurements since ISS Increment 17 (2008), with undefined impacts to crew. These measurements do not take into account the effects of hearing protection devices worn by the crew. The purpose of this paper is to provide an update on ISS noise exposure monitoring approaches and hearing conservation strategies that include acoustic dosimetry data collected since the ISS Increment 55 mission (April 2018). Future directions and recommendations for the ISS noise exposure monitoring program will also be presented, including research initiatives aimed at better defining the impact of ISS noise on crew health and performance.

scholarly journals Probiotics on the Board of the International Space Station: from the Space Experiment to the Space Production.

2020 ◽  
pp. 104-119
Author(s):  
E.V. Popova ◽  
I.V. Kutnik ◽  
A.I. Kobatov ◽  
N.B. Verbitskaya ◽  
I.V. Churilova ◽  
...  
Keyword(s):  
International Space Station ◽  
Human Body ◽  
Space Station ◽  
Experimental Results ◽  
Space Experiment ◽  
International Space ◽  
Probiotic Potential ◽  
Series Of Experiments ◽  
Space Production ◽  
The Impact

Since 2007, a series of experiments on the production and use of product with high probiotic potential is being performed on the board of the ISS in order to weaken the impact of mutagenic factors on the human body. The first research objective was to develop the technology of the microorganism emulsive cultivation under weightless conditions on the board of the ISS. The second research objective was to obtain products of microbiological origin good for maintaining the required composition of human endo-microflora. Crews of the ISS-49 through ISS-63 were involved in the research in the framework of the “Probiovit” space experiment. The paper analyzes and summarizes obtained experimental results.

scholarly journals Spatial Characterization of Microbial Communities on Multi-Species Leafy Greens Grown Simultaneously in the Vegetable Production Systems on the International Space Station

Life ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1060
Author(s):  
Mary E. Hummerick ◽  
Christina L. M. Khodadad ◽  
Anirudha R. Dixit ◽  
Lashelle E. Spencer ◽  
Gretchen J. Maldonado-Vasquez ◽  
...  
Keyword(s):  
International Space Station ◽  
Lactuca Sativa ◽  
Crop Production ◽  
Production Systems ◽  
Space Station ◽  
Amplicon Sequencing ◽  
Vegetable Production ◽  
International Space ◽  
Leafy Greens ◽  
The Impact

The establishment of steady-state continuous crop production during long-term deep space missions is critical for providing consistent nutritional and psychological benefits for the crew, potentially improving their health and performance. Three technology demonstrations were completed achieving simultaneous multi-species plant growth and the concurrent use of two Veggie units on the International Space Station (ISS). Microbiological characterization using molecular and culture-based methods was performed on leaves and roots from two harvests of three leafy greens, red romaine lettuce (Lactuca sativa cv. ‘Outredgeous’); mizuna mustard, (Brassica rapa var japonica); and green leaf lettuce, (Lactuca sativa cv. Waldmann’s) and associated rooting pillow components and Veggie chamber surfaces. Culture based enumeration and pathogen screening indicated the leafy greens were safe for consumption. Surface samples of the Veggie facility and plant pillows revealed low counts of bacteria and fungi and are commonly isolated on ISS. Community analysis was completed with 16S rRNA amplicon sequencing. Comparisons between pillow components, and plant tissue types from VEG-03D, E, and F revealed higher diversity in roots and rooting substrate than the leaves and wick. This work provides valuable information for food production-related research on the ISS and the impact of the plant microbiome on this unique closed environment.

scholarly journals Study of the impact of long-duration space missions at the International Space Station on the astronaut microbiome

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Alexander A. Voorhies ◽  
C. Mark Ott ◽  
Satish Mehta ◽  
Duane L. Pierson ◽  
Brian E. Crucian ◽  
...  
Keyword(s):  
International Space Station ◽  
Space Station ◽  
Space Missions ◽  
International Space ◽  
Long Duration ◽  
The Impact

scholarly journals Colour remote sensing of the impact of artificial light at night (I): The potential of the International Space Station and other DSLR-based platforms

2019 ◽  
Vol 224 ◽  
pp. 92-103 ◽  
Author(s):  
Alejandro Sánchez de Miguel ◽  
Christopher C.M. Kyba ◽  
Martin Aubé ◽  
Jaime Zamorano ◽  
Nicolas Cardiel ◽  
...  
Keyword(s):  
Remote Sensing ◽  
International Space Station ◽  
Space Station ◽  
Artificial Light ◽  
Light At Night ◽  
International Space ◽  
The Impact

scholarly journals OmpA Protein-Deficient Acinetobacter baumannii Outer Membrane Vesicles Trigger Reduced Inflammatory Response

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 407
Author(s):  
Jūratė Skerniškytė ◽  
Emilija Karazijaitė ◽  
Asta Lučiūnaitė ◽  
Edita Sužiedėlienė
Keyword(s):  
Inflammatory Response ◽  
Acinetobacter Baumannii ◽  
Outer Membrane ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Host Cells ◽  
Proinflammatory Response ◽  
Ompa Protein ◽  
The Impact

Multidrug resistant Acinetobacter baumannii shows a growing number of nosocomial infections worldwide during the last decade. The outer membrane vesicles (OMVs) produced by this bacterium draw increasing attention as a possible treatment target. OMVs have been implicated in the reduction of antibiotic level in the surrounding environment, transfer of virulence factors into the host cells, and induction of inflammatory response. Although the evidence on the involvement of OMVs in A. baumannii pathogenesis is currently growing, their role during inflammation is insufficiently explored. It is likely that bacteria, by secreting OMVs, can expand the area of their exposure and prepare surrounding matrix for infection. Here, we investigated the impact of A. baumannii OMVs on activation of macrophages in vitro. We show that OmpA protein present in A. baumannii OMVs substantially contributes to the proinflammatory response in J774 murine macrophages and to the cell death in both lung epithelium cells and macrophages. The loss of OmpA protein in OMVs, obtained from A. baumannii ∆ompA mutant, resulted in the altered expression of genes coding for IL-6, NLRP3 and IL-1β proinflammatory molecules in macrophages in vitro. These results imply that OmpA protein in bacterial OMVs could trigger a more intense proinflammatory response.

scholarly journals Validation of Methods to Assess the Immunoglobulin Gene Repertoire in Tissues Obtained from Mice on the International Space Station

2020 ◽  
Vol 5 (1) ◽  
pp. 2-23 ◽  
Author(s):  
Trisha A. Rettig ◽  
Claire Ward ◽  
Michael J. Pecaut ◽  
Stephen K. Chapes
Keyword(s):  
Sample Preparation ◽  
International Space Station ◽  
Gene Segment ◽  
Space Station ◽  
Gene Repertoire ◽  
Immunoglobulin Gene ◽  
Data Set ◽  
International Space ◽  
The Impact ◽  
And Storage

AbstractSpaceflight is known to affect immune cell populations. In particular, splenic B-cell numbers decrease during spaceflight and in ground-based physiological models. Although antibody isotype changes have been assessed during and after spaceflight, an extensive characterization of the impact of spaceflight on antibody composition has not been conducted in mice. Next Generation Sequencing and bioinformatic tools are now available to assess antibody repertoires. We can now identify immunoglobulin gene-segment usage, junctional regions, and modifications that contribute to specificity and diversity. Due to limitations on the International Space Station, alternate sample collection and storage methods must be employed. Our group compared Illumina MiSeq® sequencing data from multiple sample preparation methods in normal C57Bl/6J mice to validate that sample preparation and storage would not bias the outcome of antibody repertoire characterization. In this report, we also compared sequencing techniques and a bioinformatic workflow on the data output when we assessed the IgH and Igκ variable gene usage. Our bioinformatic workflow has been optimized for Illumina HiSeq® and MiSeq® datasets, and is designed specifically to reduce bias, capture the most information from Ig sequences, and produce a data set that provides other data mining options.

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